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All Journal INOTEKS : Jurnal Inovasi Ilmu Pengetahuan, Teknologi, dan Seni Journal of the Indonesian Tropical Animal Agriculture
E. Yulianti
Faculty of Animal Science, Mataram University, Jl. Majapahit No. 62 Mataram NTB 83125
Agriculture, Biological Sciences & Forestry Arts Humanities Computer Science & IT Earth & Planetary Sciences

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UTILIZATION OF POLYCLONAL ANTIBODIES PRODUCED IN LOCAL HORSES (EQUUS CABALLUS) AS A RESOURCE FOR DEVELOPMENT OF ELISA CONJUGATE TO DETECT HEPATITIS B VIRUS (HBV) SURFACE ANTIGENS Depamede, S.N.; Sriasih, M.; Yulianti, E.
Journal of the Indonesian Tropical Animal Agriculture Vol 37, No 2 (2012): (June)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.37.2.103-107

Abstract

The aim of this study was to utilize the antibody produced using Indonesia local horses (Equuscaballus) to make the conjugate of ELISA kit for detection of hepatitis B virus (HBV) surface antigen(HBsAg). The polyclonal antibodies were isolated and purified from local horses immunized repeatedlyusing isolated and purified HBsAg from Indonesia. The antibodies were conjugated with horseradishperoxidase by a modified method of Nakane and Kawaoi. The conjugate activities were performed usingthe principle of ELISA test conducted by the researchers as well as by independent laboratory.Commercial conjugate for HBsAg ELISA was used as a comparison study. The results of this studyindicated that the antibody produced from local horses can be used to make conjugates that werecomparable to commercial HBsAg ELISA kit.
PENGARUH VARIASI KONSENTRASI BAHAN ADITIF LARUTAN NANOPARTIKEL PERAK TERHADAP SIFAT ANTI-JAMUR CAT DINDING SEBAGAI APLIKASI TEKNOLOGI NANO DALAM INDUSTRI CAT DINDING Dwandaru, W.S. Brams; Putri, Z.M. Chrishar; Yulianti, E.
INOTEKS: Jurnal Inovasi Ilmu Pengetahuan,Teknologi, dan Seni Vol 20, No 1 (2016)
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (606.4 KB) | DOI: 10.21831/ino.v20i1.11268

Abstract

This research aims to (1) identify the characteristics of silver nanoparticle by using UV-Vis spectrophotometer; (2) determine the anti-fungus property of wallpaint after being added by silver nanoparticle solution; and (3) determine the effect of silver nanoparticle solution concentration towards the anti-fungus property of the wallpaint. This study begins by synthesizing the silver nanoparticle solution with concentrations of (in mM) 1, 2, 3, 4, and 5. Then as much as 80% and 20% of wall paint and silver nanoparticle solution, respectively, are mixed. The solution obtained is tested using UV-Vis pectrophotometer and followed by conducting an experiment of the anti-fungus properties of the wallpaint with silver nanoparticle solution as an additive material. The measurements are conducted three times. This research shows that concentration of the silver nanoparticle solution affects the anti-fungus properties of the wallpaint. The diameter of the anti-fungus for the concentration of (in mM) 1, 2, 3, 4, and 5 are (in cm) 1.36; 1.42; 1.45; 1.53; and 1.79, respectively. The greater the concentration, the greater the diameter of the anti-fungus. Therefore, the silver nanoparticle solution can be used to obstruct the fungus growth on the wall 
Co-Authors Dwandaru, W.S. Brams M. SRIASIH Putri, Z.M. Chrishar Sulaiman Ngongu Depamede