E. YUNIARTI
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Increasing of N-uptake by Inoculation of Diazotroph Endophytic Bacteria in Vermiculite Media DWI N. SUSILOWATI; RATIH SARASWATI; R.D HASTUTI; E. YUNIARTI
Jurnal Tanah dan Iklim (Indonesian Soil and Climate Journal) No 26 (2007): Desember 2007
Publisher : Balai Besar Penelitian dan Pengembangan Sumberdaya Lahan Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jti.v0n26.2007.%p

Abstract

Intensive selection of selected 15 isolates on N2-fixing activities and auxin production to diazotroph endophytic bacteria showed that five isolates were superior, that is KACP12 (0.2569 μmol hour-1 culture-1), KACP13 (0.3026 μmol hour-1 culture-1), KACP21 (0.4592 μmol hour-1 culture-1), KACP32 (0.3131 μmol hour-1 culture-1), and KAMG2 (0.4843 μmol hour-1 culture-1). Inoculated five superior isolates into soybean seeds in vermiculite media showed that soybean plant inoculated by KAMG2 has the highest nitrogenase specific activity compared to others and control, that is 2,54 ± 1,2 μmol hour-1 plant-1. However inoculation with KACM and KACP32 showed higher N-uptake of soybean plant. Although this research has conducted within theearly stage of soybean plant growth, it is obvious that inoculated diazotroph endophytic bacteria in vermiculite media seem to be a good method to introduce selected strain envisaging growth promoting and nitrogen fixation.