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Anik Nuryati
Department of Medical Laboratory Technology, Poltekkes Kemenkes Yogyakarta, Indonesia
Dentistry Health Professions Medicine & Pharmacology Nursing Public Health

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Uji diagnostik uji antigen sars-cov-2 pada pemeriksaan laboratorium covid-19: Studi kasus di RS Beriman Kota Balikpapan, Indonesia Dwi Hartantoro; Anik Nuryati; Siti Nuryani
PUINOVAKESMAS Vol. 2 No. 2 (2021): November
Publisher : Pusat Unggulan Iptek (PUI) Poltekkes Kemenkes Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (299.204 KB) | DOI: 10.29238/puinova.v2i2.1171

Abstract

The performance characteristics of the rapid SARS-CoV-2 antigen detection test should be evaluated and compared with the reverse transcription-polymerase chain reaction (RT-PCR) assay as the standard for diagnosis of COVID-19 cases. This research is a descriptive observational study with a cross-sectional research design. Secondary data from suspected COVID-19 patients who were examined for SARS-CoV-2 antigen and the results of RT-PCR in January - April 2021 at the Beriman Hospital of Balikpapan City, which was 336 samples. Based on the results of the study, the number of samples with an antigen-positive test for SARS-CoV-2 and a positive RT-PCR result was 114 (33.93%), the number of samples with an antigen-positive test for SARS-CoV-2 and a negative RT-PCR result was 2 (0.6%), the number of samples with positive SARS-CoV-2 antigen-negative test results and RT-PCR was 23 (6.85%), and the number of samples with SARS-negative antigen-negative test and RT-PCR results CoV -2 and negative PCR were 197 (58.63%). The result of the SARS-CoV-2 antigen sensitivity was 83.21%, while the specificity was 98.99%. The accuracy value of the SARS-CoV-2 antigen is 92.56%. The rapid antigen test used in this study has sensitivity and specificity as recommended by WHO, so it is suitable for use for COVID-19 laboratory tests.
Differences in blood creatinine levels using mono reagent which was checked immediately and which was delayed for 5 hours at room temperature Nurhidayati Nurhidayati; Ratih Hardisari; Anik Nuryati
PUINOVAKESMAS Vol. 2 No. 2 (2021): November
Publisher : Pusat Unggulan Iptek (PUI) Poltekkes Kemenkes Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (259.265 KB) | DOI: 10.29238/puinova.v2i2.1173

Abstract

Reagents used for examination must be considered for stability. Reagents made beforehand must be used immediately because the reagent that has been opened has a shorter stability period than the reagent that has not been opened. This study aims to determine the difference in creatinine levels using a mono reagent that is immediately used and which is left in for 5 hours at room temperature. This research is a pre-experimental study with a one-group pretest-posttest research design. The research sample was the remaining serum of the patients who were examined in the laboratory of Medika Sangatta Hospital as many as 40 samples. The test results were analyzed descriptively and statistically using paired sample t-test. The results of the descriptive test showed that the difference between the mean creatinine levels using immediate reagent and those experiencing indentation for 5 hours was 0.1 with the maximum value of creatinine levels using immediate reagent and those experiencing indwelling were 1.7 mg/dl and 1.6 mg/dl. While the minimum values ??are 0.9 mg / dl and 0.8 mg / dl (p=0.003). There was a difference in creatinine levels using a mono reagent which is immediately used and which has been left standing for 5 hours at room temperature so the mono reagent that has been made should be used immediately.
Co-Authors Dwi Hartantoro Nurhidayati Nurhidayati Ratih Hardisari Siti Nuryani