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Clinical and oral microbiome pattern of halitosis patients with periodontitis and gingivitis Ningsih, Diana S.; Idroes, Rinaldi; Bachtiar, Boy M.; Khairan, Khairan; Tallei, Trina E.; Kemala, Pati; Maulydia, Nur B.; Idroes, Ghazi M.; Helwani, Zuchra
Narra J Vol. 3 No. 2 (2023): August 2023
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v3i2.163

Abstract

Halitosis is caused by a bacterial proteolytic process that induces the production of volatile sulfur compounds, odor-causing gases. The aim of this study was to determine the clinical oral hygiene state and oral microbiome pattern of halitosis patients with periodontitis and gingivitis. The oral hygiene state of halitosis patients with periodontitis and gingivitis was assessed using the oral hygiene index simplified (OHI-S), decay missing filled teeth (DMFT), and tongue biofilm. The dorsum of the tongue and subgingival swabs were cultured for bacteria, and bacterial morphology was evaluated using Gram staining. Evaluation of the bacterial genus using the Bergey's systematic bacteriology diagram as a guide. A total of ten patients with periodontitis and gingivitis were included. Our data indicated that the scores of OHI-S and DMFT were different significantly between halitosis patients with periodontitis and gingivitis (both had p<0.001) while tongue biofilm score was not different between groups. On the dorsum of the tongue, periodontitis patients had a significant higher oral microbiome population (85.65x106 CFU/mL) compared to those with gingivitis (0.047x106 CFU/mL) with p=0.002. In contrast, the number of microbiomes in the subgingival had no significant different between periodontitis and gingivitis. On the dorsum of the tongue, six bacterial genera were isolated from periodontitis cases and seven genera were detected from gingivitis patients. On subgingival, 10 and 15 genera were identified from periodontitis and gingivitis, respectively. Fusobacterium, Propionibacterium, Eubacterium and Lactobacillus were the most prevalent among periodontitis cases while Porphyromonas was the most prevalent in gingivitis patients. In conclusion, although OHI-S and DMFT are different between periodontitis and gingivitis, overlapping of bacterial genera was detected between periodontitis and gingivitis cases.
Oral microbiome: A paradigm shift in dental diagnosis Megantoro, Aryo; Alphianti, Likky T.; Gunawan, Paulina N.; Bachtiar, Endang W.; Bachtiar, Boy M.
Narra X Vol. 3 No. 1 (2025): April 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narrax.v3i1.197

Abstract

For decades, the diagnosis of oral diseases primarily relied on clinical symptoms presented by patients. This traditional approach, which included visual inspection, palpation, and assessment of discomfort, provided limited insights into the underlying microbiological factors contributing to oral diseases. Recent studies have identified specific microorganisms believed to play pivotal roles in oral disease development, particularly in conditions such as dental caries, periodontal disease, and pulpitis. The challenge lies in the dynamic nature of the oral microenvironment, where the microbial community can shift rapidly due to changes in diet, hygiene practices, and overall health, complicating attempts to establish direct causative links between specific pathogens and oral diseases. The aim of this study was to explore the role of the oral microbiome in advancing dental diagnostics and to assess how integrating microbial analysis can improve early detection and personalized treatment of oral diseases. Research into the oral microbiome has brought about a paradigm shift in understanding dental diagnostics. Advances in molecular biology techniques, such as next-generation sequencing (NGS) and polymerase chain reaction (PCR), have enabled more detailed examination of microbial communities within the oral cavity. This shift from a purely symptom-based diagnostic approach to one that incorporates microbial analysis represents a significant advancement in dental care. For instance, identifying specific bacterial profiles associated with caries could facilitate the implementation of personalized preventive measures, such as tailored oral hygiene regimens or dietary recommendations. Moreover, integrating microbiological data into clinical practices could lead to improved diagnostics for conditions that are traditionally difficult to assess. By adopting a more comprehensive view that includes microbial assessments, clinicians can better understand the interplay between oral microbiota and systemic health, as oral diseases are often linked to broader health issues. Another challenge is that this approach requires interdisciplinary collaboration among dental practitioners, microbiologists, and public health experts. This collaboration is essential to translate abstract microbiological findings into practical diagnostic indicators that can be utilized in clinical settings. Furthermore, with the advent of new technologies, maintaining accurate interpretations of microbiome data presents another layer of complexity, as variations in sample collection, processing, and analysis can lead to differing results. In summary, the elevated role of the oral microbiome in dental diagnostics marks a significant transition from traditional, symptom-focused approaches to more holistic methodologies that consider the underlying microbial communities. By leveraging advanced technologies, dental practitioners can enhance their diagnostic capabilities, leading to improved outcomes for patients suffering from various oral diseases.