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Retrieval of Separated NiTi Instrument in Severe Curved Canal using an Ultrasonic Device: A Case Report Putranto, Aditya W.; Maharti, Ike D.; Megantoro, Aryo; Ricardo, Shalina
Journal of Dentistry Indonesia Vol. 25, No. 3
Publisher : UI Scholars Hub

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Abstract

Objective: We describe how to assess the degree of canal curvature and manage a separated instrument using an ultrasonic device. Case Report: A 24-year-old female was referred by a general dentist for an accidentally separated Protaper F2 instrument. The mesiobuccal canal was enlarged and the separated instrument could be visualized via the dental operating microscope. Preoperative radiography revealed a separated endodontic instrument at the middle to apical third of the mesiobuccal canal. Mesiobuccal canal curvature measured 66°. After rubber dam isolation, a specific ultrasonic tip and the dental operating microscope were used to gain access around the separated instrument until it loosened. The separated instrument was agitated and accidentally sucked into the high-speed suction device. The canal was evaluated with higher magnification via the dental operating microscope and postoperative radiographs were taken to confirm removal. Conclusion: Retrieval of a separated instrument in the curved canal is effective using a specific ultrasonic device plus a dental operating microscope
Effect of varnish coating agent application on surface hardness of glass ionomer cement after immersion in carbonated drinks Arsanti, Natasya; Megantoro, Aryo; Meidyawati, Ratna
Padjadjaran Journal of Dentistry Vol 35, No 1 (2023): March 2023
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol35no1.33139

Abstract

ABSTRACTIntroduction: Glass ionomer cement is a material for treating dental caries. The disadvantage of glass ionomer cement is that if it comes into contact with acids before maturation, there will be a decrease in mechanical properties which is indicated by a decrease in surface hardness. Surface hardness has a relationship with aesthetics and resistance to scratches that can cause fractures. Coating agents like varnishes are required for glass ionomer cement to protect them from liquids with low pH level, such as carbonated drinks. Unfortunately many dentists neglected this procedure. The purpose of this research was to compare the surface hardness of glass ionomer cement which is protected and not protected by coating agent when its maturation is in contact with acids, to emphasize the importance of this procedure. Methods: This research was a laboratory experimental study. Samples consist of 24 glass ionomer cement specimens (GC Fuji IX GP EXTRA). Glass ionomer cement specimens were divided into 4 treatment groups. The first group was not applied with varnish and stored without immersion, the second group was applied with varnish and stored in artificial saliva, the third group was applied with varnish and soaked in carbonated drinks, and the last group was not applied with varnish and was immersed in carbonated drinks. The data that has been collected was processed and analyzed using the computer-based application IBM SPSS Statistics version 23.0. Results: There was a significant increase in the surface hardness of glass ionomer cement in all treatment groups. There was a significant difference in the mean surface hardness of glass ionomer cement after immersion in carbonated drinks between the groups that were applied with varnish coating agent and that without application of varnish coating agent. Conclusion: The surface hardness of glass ionomer cement restoration which was applied with varnish coating agent has a higher value compared to those not applied with varnish coating agent after immersion on carbonated drinks.Keywords: carbonated drinks, glass ionomer cement, microhardness test; saliva, varnish coating agent
Oral microbiome: A paradigm shift in dental diagnosis Megantoro, Aryo; Alphianti, Likky T.; Gunawan, Paulina N.; Bachtiar, Endang W.; Bachtiar, Boy M.
Narra X Vol. 3 No. 1 (2025): April 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narrax.v3i1.197

Abstract

For decades, the diagnosis of oral diseases primarily relied on clinical symptoms presented by patients. This traditional approach, which included visual inspection, palpation, and assessment of discomfort, provided limited insights into the underlying microbiological factors contributing to oral diseases. Recent studies have identified specific microorganisms believed to play pivotal roles in oral disease development, particularly in conditions such as dental caries, periodontal disease, and pulpitis. The challenge lies in the dynamic nature of the oral microenvironment, where the microbial community can shift rapidly due to changes in diet, hygiene practices, and overall health, complicating attempts to establish direct causative links between specific pathogens and oral diseases. The aim of this study was to explore the role of the oral microbiome in advancing dental diagnostics and to assess how integrating microbial analysis can improve early detection and personalized treatment of oral diseases. Research into the oral microbiome has brought about a paradigm shift in understanding dental diagnostics. Advances in molecular biology techniques, such as next-generation sequencing (NGS) and polymerase chain reaction (PCR), have enabled more detailed examination of microbial communities within the oral cavity. This shift from a purely symptom-based diagnostic approach to one that incorporates microbial analysis represents a significant advancement in dental care. For instance, identifying specific bacterial profiles associated with caries could facilitate the implementation of personalized preventive measures, such as tailored oral hygiene regimens or dietary recommendations. Moreover, integrating microbiological data into clinical practices could lead to improved diagnostics for conditions that are traditionally difficult to assess. By adopting a more comprehensive view that includes microbial assessments, clinicians can better understand the interplay between oral microbiota and systemic health, as oral diseases are often linked to broader health issues. Another challenge is that this approach requires interdisciplinary collaboration among dental practitioners, microbiologists, and public health experts. This collaboration is essential to translate abstract microbiological findings into practical diagnostic indicators that can be utilized in clinical settings. Furthermore, with the advent of new technologies, maintaining accurate interpretations of microbiome data presents another layer of complexity, as variations in sample collection, processing, and analysis can lead to differing results. In summary, the elevated role of the oral microbiome in dental diagnostics marks a significant transition from traditional, symptom-focused approaches to more holistic methodologies that consider the underlying microbial communities. By leveraging advanced technologies, dental practitioners can enhance their diagnostic capabilities, leading to improved outcomes for patients suffering from various oral diseases.
Matrix Metalloproteinase-9 (MMP-9) Levels in Dentinal Fluid as a Diagnostic Biomarker for Irreversible Pulpitis Megantoro, Aryo; Wairooy, Valeria Widita; Meidyawati, Ratna; Bachtiar, Endang Winiati
Journal of Dentistry Indonesia
Publisher : UI Scholars Hub

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Abstract

Objective: Pulpitis is an inflammatory condition of the dental pulp. The inflammatory status can be accurately assessed by histological examination. Biomarkers, such as matrix metalloproteinases (MMPs), are effective indicators of inflammation severity. Among these, matrix metalloproteinase-9 (MMP-9) has emerged as a key mediator of inflammatory processes associated with pulpitis. However, its clinical application as a biomarker remains limited owing to challenges in its extraction. This study aimed to evaluate the effectiveness of MMP-9 extracted from the dentinal fluid as a biomarker for pulpitis. Methods: Dentinal fluid was collected using the blunt tip of a sterile paper point, following clean excavation of the cavities. The paper point was placed in a sterile tube containing phosphate-buffered saline (PBS) and stored at −20 ºC. MMP-9 levels were analyzed using enzyme-linked immunosorbent assay (ELISA). Results: MMP-9 was detected in three of four patients diagnosed with irreversible pulpitis. Kruskal-Wallis test revealed a statistically significant difference in MMP-9 levels in the irreversible pulpitis group (p < 0.05). Conclusion: MMP-9 in dentinal fluid is a potential biomarker for irreversible pulpitis. However, further research is required to validate their diagnostic utility in clinical practice.