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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Engineering Environmental Science Public Health

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Biodegradable plastics from linier low-density polyethylene and polysaccharide: The influence of polysaccharide and acetic acid Dwi Wahini Nurhajati; Bidhari Pidhatika; Syaiful Harjanto
Majalah Kulit, Karet, dan Plastik Vol 35, No 1 (2019): Majalah Kulit, Karet, dan Plastik
Publisher : Center for Leather, Rubber, and Plastic Ministry of Industry, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1755.602 KB) | DOI: 10.20543/mkkp.v35i1.4874

Abstract

Global problems associated with conventional, non-biodegradable plastics have urged the society to use more eco-friendly biodegradable plastics. In this study, linear low-density polyethylene (LLDPE) was co-compounded with cassava-based thermoplastic starch (TPS) to prepare biodegradable plastics (i.e. plastics that can be degraded by microbes), in which three different LLDPE/TPS ratios were studied. Acetic acid was used to hydrolyze the polysaccharides by breaking the branched amylopectin that causes the TPS-containing composites brittle and stiff. The biodegradation properties of the LLDPE/TPS composites were determined by observing the level of microbial growth on the sample surface after incubation with potato dextrose agar medium that was inoculated with Penicillium sp. and Aspergillus niger. Burial test in a humid composting medium was also performed to validate the biodegradation properties. The mass change (%) was calculated in relative to the initial mass before burial test. The physical properties (tensile strength and elongation at break) of the bioplastics were determined using universal testing machine before and after burial treatment. The morphology of the sample surface was evaluated using scanning electron microscopy. The results showed that the microbial growth increases with increasing TPS content. Negative mass changes were observed on all samples that contain TPS, with increase in the magnitude with increasing TPS content.  The tensile strength tends to increase in the first 28 days of burial period in a composting medium then decreases and plateaus, while the elongation at break decreases with increasing burial period. Moreover, samples that contain acetic acid showed less microbial attachment and less biodegradation compared to samples that does not contain acetic acid.
AKTIVITAS ANTIBAKTERI BERBAGAI POLIMER ANTIMIKROBA TERHADAP BAKTERI YANG DIISOLASI DARI PERMUKAAN LENSA KONTAK SECARA IN VITRO Nur Fathurahman Ridwan; Anna Rakhmawati; Bidhari Pidhatika
Kingdom (The Journal of Biological Studies) Vol 5, No 7 (2016): Jurnal Biologi Vol 5 No 7 tahun 2016
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/kingdom.v5i7.6031

Abstract

Penelitian ini bertujuan untuk mengetahui nilai daya hambat, potensi relatif dan nilai kadar hambat minimum dari polimer Poli L-Lisin (PLL), Peptida Antimikroba (PAM), Poli L-Lisin-graft- Poli Etilen Glikol (PLL-g-PEG), dan Poli L-Lisin-graft-Poli 2-Metil-2-Oxazolin (PLL-g-PMOXA) terhadap bakteri yang telah diisolasi dari lensa kontak. Penelitian ini merupakan penelitian eksperimen rancangan acak lengkap (RAL) 2 faktor dengan 5 ulangan. bakteri yang diujikan adalah Staphylococcus sp., Neisseria sp., Aeromonas sp, dan Enterobacter sp. Data dianalisis secara deskriptif. Hasil penelitian menunjukkan PLL dan AMP kadar lebih rendah (50 ppm-250 ppm) dibandingkan dengan PLL-g-PEG dan PLL-g-PMOXA (250 ppm-500 ppm) pada berbagai bakteri yang diujikan. PLL dan AMP memiliki potensi relatif yang lebih tinggi dibandingkan dengan PLL-g-PEG dan PLL-g-PMOXA jika dibandingkan dengan kloramfenikol. Terdapat kebocoran sel pada membran plasma dari sel bakteri yang diujikan dari perlakuan polimer PLL, AMP dan PLL-g-PEG, PLL-g-PMOXA sedangkan kerusakan dinding sel dari nilai N-Ag memiliki perbedaan hasil perlakuan PLL dan AMP cenderung menyebabkan kerusakan dinding sel namun polimer PLL-g-PEG dan PLL-g-PMOXA cenderung tidak menyebabkan kerusakan peptidoglikan.Kata kunci: Antibakteri, AMP, daya hambat, Lensa Kontak.
Co-Authors Anna Rakhmawati Dwi Wahini Nurhajati Nur Fathurahman Ridwan Syaiful Harjanto