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All Journal Prosiding Scripta Biologica BioEksakta : Jurnal Ilmiah Biologi Unsoed
Ardhini R Maharning
Universitas Jenderal Soedirman
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Veterinary

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EFFECTS OF PLANT LITTER DIVERSITY AND QUALITY ON SOIL MICRO- AND MESO-FAUNA Maharning, Ardhini R; Irianto, Agus
Prosiding Vol 3, No 1 (2012)
Publisher : Prosiding

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Abstract

Tujuan dari penelitian ini adalah untuk mengetahui karakter padi gogo toleran kekeringan dengan daya hasil tinggi pada kondisi kadar air tanah rendah pada sistem tanam intercrops dengan rumput. Penelitian dilakukan di lahan tadah hujan Desa Banjaranyar dengan menggunakan Rancangan Petak Terbagi diulang tiga kali. Petak utama terdiri atas tanpa rumput, rumput gajah dan sereh serta anak petak terdiri atas vartietas Situ Patenggang, Kalimutu, Danau Gaung, Jatiluhur dan Cisokan. Pada kondisi kadar air tanah rendah (
Isolation and Characterization of Plant Growth Promoting Rhizobacteria from Ipomoea sp. Rhizospheres Growing in Iron Sand Soil niharoh nurainy; Oedjijono Oedjijono; Ardhini Rin Maharning
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 1 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (587.803 KB) | DOI: 10.20884/1.bioe.2020.2.1.1845

Abstract

Iron sand field, is mostly found along the Indonesia coast. It has low organic matter, contains 38-59% iron (Fe) and sand particles. These characteristics can be called as extreme environments, however there are bacteria capable of growing and surviving in such habitats. Several genera are known as PGPR agents such as Rhizobium, Azospirillum, Azotobacter and Pseudomonas. The research objectives were to measure total population of bacteria from rhizosphere of Ipomoea sp. in iron sand soils, to investigate the ability of bacterial isolates capable of fixing nitrogen, solubilizing phosphate, and producing plant growth hormone such as IAA, and to identify plant growth promoting rhizobacteria isolated from plant rhizospheres candidates growing in iron sand soils. Isolation on NA medium showed that the population were ranged from 1.59 x 105 to 5.2 x 105 CFU.g-1. There were 22 bacterial isolates originated from the media of Ashby, Caceres, and Pikovskaya. Six isolates (A4, A10, C10, P2, P3, and P4) showed high ability to fix nitrogen, solubilize phosphate, and produce IAA. Isolate P4 grew in nitrogen fixing and phosphate solubilizing assay as well as IAA producing. It showed high value of phosphate index (275 mm). Bacterial identification indicated that four isolates (C10, P2, P3, P4) were species members of genus Bacillus and two isolates (A4, A10) were identified as species members of Actinomycetes.
NEMATODE COMMUNITY RESPONSE TO VARIED PROPORTION OF DECOMPOSING PLANT LITTER Anita Rachman Sholeha; Ardhini Rin Maharning; Erie Kolya Nasution
Scripta Biologica Vol 4, No 3 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (560.219 KB) | DOI: 10.20884/1.sb.2017.4.3.588

Abstract

The research objectives were to investigate nematode community response to decomposing plant litter of varying proportions, and to measure the litter decomposition rate. This greenhouse experiments included three treatments of decomposing plant litter with proportions of 30:70, 50:50, and 70:30 (leaf litter to stem-paddy litter, weight to weight), and two controls (commercial organic fertilizers and no-organic matter). We applied the prepared plant litter and organic fertilizers on the surface of the agricultural sandy soil. Soil samples were taken after two, seven, 14, 28, 49, and 77 days to follow nematode temporal changes. Nematodes were assigned to functional groups and family level of a taxon. We used Anova to test the significance of nematode abundance among the treatments, exponential decay model to measure decomposition rates of the plant litter, and canonical correspondence analysis to investigate the correlation of varying plant proportion and nematode community composition. The results showed that nematode functional groups and family did not differ among soils of various plant litter proportions. Bacterivorous Rhabditidae and Cephalobidae, fungivorous Aphelenchidae, and fungivorous-root feeder Tylenchidae inhabited plant litter amended soils. Their abundance, however, differed significantly (p<0.001) and revealed two temporal patterns, i.e., linear (50:50) and unimodal (30:70 and 70:30). No nematodes were observed in organic fertilizer amended soil and no-organic matter soil. Nematode community composition changed over time according to the litter proportion, in which by the end of experiments, community of 50:50 and 30:70 were the least similar (r: 0.57 of axis-1 and 0.54 of axis-2, CPV: 89.06). We concluded that all litter proportions retained similar quality and supported the growth of similar nematode groups. The litter proportion, however, appeared to influence nematode abundance, their temporal patterns, and community compositions.
Isolation and Characterization of Plant Growth Promoting Rhizobacteria from Ipomoea sp. Rhizospheres Growing in Iron Sand Soil nurainy, niharoh; Oedjijono, Oedjijono; Maharning, Ardhini Rin
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 1 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.1.1845

Abstract

Iron sand field, is mostly found along the Indonesia coast. It has low organic matter, contains 38-59% iron (Fe) and sand particles. These characteristics can be called as extreme environments, however there are bacteria capable of growing and surviving in such habitats. Several genera are known as PGPR agents such as Rhizobium, Azospirillum, Azotobacter and Pseudomonas. The research objectives were to measure total population of bacteria from rhizosphere of Ipomoea sp. in iron sand soils, to investigate the ability of bacterial isolates capable of fixing nitrogen, solubilizing phosphate, and producing plant growth hormone such as IAA, and to identify plant growth promoting rhizobacteria isolated from plant rhizospheres candidates growing in iron sand soils. Isolation on NA medium showed that the population were ranged from 1.59 x 105 to 5.2 x 105 CFU.g-1. There were 22 bacterial isolates originated from the media of Ashby, Caceres, and Pikovskaya. Six isolates (A4, A10, C10, P2, P3, and P4) showed high ability to fix nitrogen, solubilize phosphate, and produce IAA. Isolate P4 grew in nitrogen fixing and phosphate solubilizing assay as well as IAA producing. It showed high value of phosphate index (275 mm). Bacterial identification indicated that four isolates (C10, P2, P3, P4) were species members of genus Bacillus and two isolates (A4, A10) were identified as species members of Actinomycetes.
Co-Authors Agus Irianto Anita Rachman Sholeha Erie Kolya Nasution niharoh nurainy nurainy, niharoh Oedjijono Oedjijono Oedjijono Oedjijono, Oedjijono