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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology BIO-SITE |BIOLOGI Sains Terapan Biosaintifika: Journal of Biology & Biology Education BioEksakta : Jurnal Ilmiah Biologi Unsoed Molekul: Jurnal Ilmiah Kimia
Oedjijono Oedjijono, Oedjijono
Universitas Jendral Soedirman
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Immunology & microbiology Materials Science & Nanotechnology Veterinary

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AKTIVITAS PELARUTAN FOSFAT ISOLAT Azospirillum spp. ASAL LAHAN PASIR BESI Wuryanto, Sodik; Oedjijono, Oedjijono
BIO-SITE |BIOLOGI Sains Terapan Vol 1, No 01 (2015): Bio-Site
Publisher : BIO-SITE |BIOLOGI Sains Terapan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Iron sand lands have a low organic matter content as it contains of Iron (Fe) found nearly all over the coastal or beach in Indonesia. Phosphorous is one of macronutrient with considered role for plant promotion. One group of bacteria that are in an environment of rooting plants on land sand iron was Azospirillum spp. The purpose of this study was is to know the phosphate solubilizing activities of Azospirillum spp. isolates from land sand iron. The results showed that some isolates of Azospirillum spp. origin of iron sand land capable of solubilizing phosphate in qualitative and quantitative.   Key words: Azospirillum spp., phosphate, solubilization, iron sand
Isolation and Characterization of Plant Growth Promoting Rhizobacteria from Ipomoea sp. Rhizospheres Growing in Iron Sand Soil nurainy, niharoh; Oedjijono, Oedjijono; Maharning, Ardhini Rin
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 1 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.1.1845

Abstract

Iron sand field, is mostly found along the Indonesia coast. It has low organic matter, contains 38-59% iron (Fe) and sand particles. These characteristics can be called as extreme environments, however there are bacteria capable of growing and surviving in such habitats. Several genera are known as PGPR agents such as Rhizobium, Azospirillum, Azotobacter and Pseudomonas. The research objectives were to measure total population of bacteria from rhizosphere of Ipomoea sp. in iron sand soils, to investigate the ability of bacterial isolates capable of fixing nitrogen, solubilizing phosphate, and producing plant growth hormone such as IAA, and to identify plant growth promoting rhizobacteria isolated from plant rhizospheres candidates growing in iron sand soils. Isolation on NA medium showed that the population were ranged from 1.59 x 105 to 5.2 x 105 CFU.g-1. There were 22 bacterial isolates originated from the media of Ashby, Caceres, and Pikovskaya. Six isolates (A4, A10, C10, P2, P3, and P4) showed high ability to fix nitrogen, solubilize phosphate, and produce IAA. Isolate P4 grew in nitrogen fixing and phosphate solubilizing assay as well as IAA producing. It showed high value of phosphate index (275 mm). Bacterial identification indicated that four isolates (C10, P2, P3, P4) were species members of genus Bacillus and two isolates (A4, A10) were identified as species members of Actinomycetes.
INOKULASI RIZOBAKTERIA PADA TANAMAN CABAI (Capsicum annum L.) DI TANAH SALIN anti, Monica Widi; Oedjijono, Oedjijono; Proklamasiningsih, Elly
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.2.2140

Abstract

A red chili plant (Capsicum spp.) is one of the horticultural commodities in Indonesia which has good economic value, so it has priority to be developed. Agriculture practices in Java island have partly switched to coastal areas which have characteristics of saline soils. Saline soils are alkaline and nutrient-poor, especially essential nutrients such as P and N. One way to restore fertility in saline soils used beneficial and fertilizing bacteria such as PGPR. Plant growth-promoting rhizobacteria (PGPR) are free-living, soil-borne bacteria, which are capable of enhancing the growth of the plant either directly or indirectly. The objectives of this research were to determain the effect of PGPR isolates to the growth of red chili plants in saline sandy soil and to determine the best PGPR isolate capable to improve the growth of red chili plant in saline sandy soil. The result showed that the inoculation of rhizobacteria significantly affected to plant height and root length of the red chili plant (Capsicum annum). The combination of 3 PGPR isolates (Azospirillum PSA 10, Azotobacter PSA 8, and Marinococcus PSA 1) was the best result in increasing the height and root length of the chili plant (Capsicum annum).
Isolasi dan Uji Resistensi Bakteri Endofit Eceng Gondok Terhadap Krom Nurmalasari, Ade; Oedjijono, Oedjijono; Lestari, Sri
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.2.2142

Abstract

Endophytic bacteria are bacteria that live in plant tissues and are not pathogenic to the host. One of endophytic bacterial host is water hyacinth. Water hyacinth could accumulate heavy metals, one of which is chrome. The purpose of this study was to obtain endophytic bacteria of water hyacinth (Eichhornia crassipes Mart.), to identify the endophytic bacteria of water hyacinth, and to determine the resistance of endophytic bacteria of water hyacinth to Chrome (Cr). The research method used was a survey with a purposive random sampling technique. Stages of research include root sampling, sterilization of root samples using 2% NaOCl3, isolation of water hyacinth endophytic bacteria on agar nutrient medium (NA), characterization of bacterial endophytes and resistance test of water hyacinth bacteria to chromium. Based on the results of the study, 8 isolates of endophytic bacteria were isolated from the roots of water hyacinth. Based on Bergey's Manual Determinative of Bacteriology, 6 bacterial isolates (E1, E2, E3, E5, E6, E8) were identified as Bacillus and 2 isolates (E4, E7) were identified as Pseudomonas. All water hyacinth endophytic bacteria obtained were resistant to chromium to a concentration of 750 mg/L.
Pengaruh Penambahan Prebiotik Inulin dan Fruktooligosakarida (FOS) terhadap Pertumbuhan Probiotik Bifidobacterium sp. Bb2E Isnasari, Azma Nurizqi; Kusharyati, Dyah Fitri; Oedjijono, Oedjijono
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 3 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3385

Abstract

Human health is very closely related to the condition of the human digestive tract since the beginning of life. The diversity of microorganisms found in the human digestive tract is very diverse, consisting of 300-500 different species of bacteria to increase the working power of nutrition. Prebiotics such as inulin and FOS and probiotics such as Bifidobacteria are aspects that can be added for the increase of nutrition. Optimal bacterial growth can be seen in the bacterial growth curve. The research problems were how the effect of prebiotic inulin and FOS on the growth of Bifidobacterium sp. Bb2E, the amount of incubation time needed to support the growth of Bifidobacterium sp. Bb2E, and how the interaction between prebiotic types and incubation times on the growth of Bifidobacterium sp. BB2E. The purposes of this study were to study the effect of prebiotic inulin and FOS on the growth of Bifidobacterium sp. Bb2E, to know the optimal incubation time of Bifidobacterium sp. Bb2E, and to know the interaction between prebiotic types and incubation times on the growth of Bifidobacterium sp. BB2E. This research was conducted using a completely randomized design (CRD) with factorial patterns. The main parameter measured was the population of Bifidobacterium sp. Bb2E, and the additional parameters measured were the pH level and the value of lactic acid titrated. The independent variable discussed in this study is prebiotic estimation on the medium, while the dependent variable considered is the population of Bifidobacterium sp. BB2E. The results of this study showed that the addition of inulin and fructooligosaccharide prebiotic at different incubation times had a significant effect on the growth of Bifidobacterium sp. Bb2E. The best treatment was a combination of inulin + FOS at incubation time of 18 hours with an optical density value was 1,794 and a total population density was 2,44x1010 CFU/mL.
Eksplorasi Bakteri Diazotrof dari Rizosfer Tanaman Bawah Merah (Allium ascalonicum L.) di Brebes, Jawa Tengah Amalia, Dwi Ayu Lutfiani; Oedjijono, Oedjijono; Purwanto, Purwanto
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 3 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3480

Abstract

Penelitian ini bertujuan untuk mengeksplorasi bakteri diazotrof yang mampu menambat nitrogen dan menghasilkan hormon IAA dengan cara menyeleksinya dari rizosfer tanaman bawang merah di Brebes, Jawa Tengah. Penelitian dilakukan selama 6 bulan (Januari-Juni 2020). Penelitian ini terdiri dari pengambilan sampel tanah perakaran bawang merah di Brebes, Jawa Tengah, isolasi bakteri tanah pada medium Yeast Mannitol Agar+Congo red, Ashby, dan Caceres, uji produksi IAA metode Salkowski, uji kemampuan penambatan nitrogen metode Kjeldahl, dan identifikasi bakteri. Sebanyak sembilan isolat bakteri diazotrof yang mampu menghasilkan IAA telah berhasil diisolasi. Kesembilan isolat tersebut mampu menghasilkan IAA dengan konstentrasi antara 3,05-3,51 ppm, dengan isolat LAR3 sebagai penghasil IAA tertinggi. Hasil perhitungan kemampuan menambat nitrogen bebas dengan metode Kjeldahl dari 6 isolat terbaik penghasil IAA, mampu menghasilkan konsentrasi berkisar antara 3,15-88,55 ppm, dengan isolat LAR5 sebagai penghasil nitrogen tertinggi. Hasil identifikasi menunjukkan kesembilan isolat bakteri yang didapatkan termasuk dalam 3 kelompok bakteri yang berbeda, yaitu empat isolat termasuk spesies anggota genus Rhizobium (isolat LAR3,LAR5,LBR1,dan LCR3), tiga isolat adalah spesies anggota genus Azospirillum (isolat LAA4,LAA5, dan LCA1), dan dua isolat termasuk spesies anggota genus Azotobacter (isolat LBZ2 dan LBZ3).
Karakterisasi dan Pengaruh Senyawa Antibakteri Streptomyces spp. dalam Menghambat Pertumbuhan Vibrio cholerae Andani, Ajeng Putri Retno; Oedjijono, Oedjijono; Ryandini, Dini
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 5 No 4 (2023): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2023.5.4.4726

Abstract

Penelitian ini bertujuan untuk mengetahui kemampuan Streptomyces spp. yang diisolasi dari sedimen mangrove dalam menghambat pertumbuhan bakteri Vibrio cholerae, mengetahui kekuatan senyawa antibakteri yang dihasilkan oleh Streptomyces spp. terhadap V. cholerae, dan mengetahui karakteristik dari senyawa antibakteri yang dihasilkan oleh Streptomyces spp. Tahap penelitian meliputi penapisan isolat bakteri Streptomyces spp. (SA34, SA37, SA40, SAE4034) yang menghambat V. cholerae, produksi senyawa antibakteri, uji aktivitas antibakteri dengan metode Kirby Bauer, penentuan nilai MIC (%), pemisahan senyawa antibakteri dengan metode TLC, uji fitokimia, uji bioautografi, uji aktivitas senyawa antibakteri pada variasi suhu (40oC, 60oC. 80oC, 100oC) dan pH (2, 4, 6, 9), serta uji konfirmasi karakter bakteri. Data dianalisis secara deskriptif. Hasil penapisan menunjukkan bahwa penghambatan terhadap pertumbuhan V. cholerae tertinggi ditunjukkan oleh isolat SAE4034. Ekstrak kasar mampu menghambat V. cholerae dengan diameter zona hambat 11,5-17,5 mm; nilai MIC 30%, dan menghasilkan senyawa bioaktif dengan nilai Rf berkisar antara 0,31-0,70. Senyawa yang mampu menghambat memiliki nilai Rf 0,43 dan senyawa tersebut termasuk golongan alkaloid. Perlakuan suhu 40oC tetap tinggi dengan zona hambat 14 mm dan aktivitas penghambatannya menurun sejalan dengan perlakuan suhu yang lebih tinggi sampai 100oC. Perlakuan pH 6 menunjukkan aktivitas penghambatan tertinggi dengan zona hambat 16 mm, sebaliknya pada pH 2, 4, dan 9 aktivitas antibakteri ekstrak kasar menurun.
Bioremediation of Cadmium (Cd) in Batik Wastewater Using Different Carrier Media Containing Rhizobacteria Lestari, Sri; Oedjijono, Oedjijono; Santoso, Slamet; Kusharyati, Dyah Fitri; Fathurrohim, Ramadi Habib
Biosaintifika: Journal of Biology & Biology Education Vol 15, No 1 (2023): April 2023
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i1.37683

Abstract

Batik wastewater is very dangerous for the environment and health because it contains heavy metals, such as Cadmium (Cd), derived from naphthol and indigo sol dyes. Rhizobacteria have great potential to detoxify Cd on a laboratory scale. Therefore, they should be packaged in carrier media to ensure their long-term viability in the field. The carrier media used were peat, sawdust, and tofu solid waste. This research aimed to determine the most potential of three rhizobacteria as tolerant to Cd from 11 isolates, the best carrier media that can maintain rhizobacteria viability after freeze-dried, and the effectiveness of carrier media containing potential rhizobacteria in reducing Cd in batik wastewater. Furthermore, an experimental method with completely randomized and split-plot designs was used. Rb1, Rb3, and Rb6 were the most Cd-tolerant of the three rhizobacteria isolates tested. For the viability of each isolate, tofu solid waste and peat were the best carrier media at Rb, while sawdust and tofu solid waste were the best at Rb3 and peat at Rb6. Isolate Rb3C3 had the highest percentage value of degrading Cd at 85.1%, while others had less than 80%. Isolate rhizobacteria with a carrier media makes it easy to apply on a field scale because it already contains a source of nutrients for bacterial growth and the packaging has a longer shelf life.
Isolasi dan Karakterisasi Rhizobakteri Bawang Merah dari Lahan salin di Kabupaten Brebes sebagai Agen Pemacu Pertumbuhan Tanaman Amalia, Dwi Ayu Lutfiani; Oedjijono, Oedjijono; Aziz, Saefudin
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 7 No 2 (2025): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2025.7.2.15864

Abstract

Salinitas tanah merupakan salah satu kendala utama dalam budidaya bawang merah (Allium ascalonicum L.), terutama di wilayah pesisir. Salah satu pendekatan yang dapat digunakan untuk mendukung pertumbuhan tanaman di bawah tekanan salin adalah dengan memanfaatkan Plant Growth-Promoting Rhizobacteria (PGPR). Penelitian ini bertujuan untuk mendapatkan isolat bakteri dari rizosfer bawang merah yang mampu tumbuh di tanah salin dan memiliki karakteristik sebagai PGPR. Sebanyak lima isolat bakteri, yaitu K4K3, K3AM1, K4IS1, K3RZ1, dan K3IS4 berhasil diisolasi dari tanah rizosfer bawang merah dan mampu tumbuh pada salinitas 3–8% NaCl. Semua isolat menunjukkan kemampuannya sebagai kandidat PGPR yaitu mampu menambat nitrogen, melarutkan fosfat dan kalium, menghasilkan hormon Indole -3-acetic Acid (IAA), serta memproduksi siderofor. Uji antagonis antar isolat menunjukkan bahwa empat isolat bersifat sinergis, kecuali interaksi antara K3RZ1 dan K3AM1 yang saling menghambat. Isolat yang bersifat sinergis diuji kemampuannya dalam fiksasi nitrogen secara kuantitatif menggunakan metode Acetylene Reduction Assay (ARA), dengan hasil aktivitas nitrogenase sebesar 0,044 μmol C₂H₄/mL/jam untuk isolat K4IS1 dan 0,066 μmol C₂H₄/mL/jam untuk isolat K3IS4. Hasil uji kuantitatif untuk sifat lainnya menunjukkan kemampuan pelarutan fosfat hingga 16,04 µg/mL dan produksi IAA hingga 2,01 µg/mL. Berdasarkan Bergey’s Manual of Determinative Bacteriology kelima isolat teridentifikasi sebagai Kocuria rosea (K4K3), Bacillus subtilis (K3AM1), Kocuria rhizophila (K4IS1), Rhizobium sp. (K3RZ1), dan Pseudomonas fluorescens (K3IS4). Berdasarkan karakter fungsional dan sinergisme antar isolat, beberapa isolat potensial untuk dikembangkan sebagai bioinokulan dalam mendukung pertumbuhan bawang merah di tanah salin. Kata kunci : Bawang merah (Allium ascalonicum L.), IAA, nitrogenase, PGPR, tanah salin
Regional Variations in rpoB Gene Mutations and Their Association with Rifampicin Resistance in Mycobacterium tuberculosis Widodo, Widodo; Wahyono, Daniel Joko; Oedjijono, Oedjijono; Pramono, Hendro
Molekul Vol 20 No 1 (2025)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2025.20.1.13215

Abstract

ABSTRACT. The increasing incidence of tuberculosis (TB) remains a global challenge, driven by the emergence of Mycobacterium tuberculosis resistance to antituberculosis drugs. This study investigated the relationship between rpoB gene mutations, rifampicin resistance levels, and the geographic origin of multidrug-resistant M. tuberculosis (MDR-TB) isolates. A total of 30 MDR-TB isolates were collected from the Central Java Provincial Health and Calibration Testing Laboratory between January and December 2023. Rifampicin resistance levels ranged from 5 to 80 µg/mL and were assessed using the microculture method, while DNA sequencing identified rpoB mutations. The results showed that rifampicin exposure significantly influenced rpoB mutation frequencies, with Ser531Leu (50%), His526 (16.7%), Leu511 (6%), Leu533 (6%), and Gln513 (6%) being the most common. Geographic variations were observed, with isolates from the ex-residencies Pekalongan, Pati, and Banyumas Residencies harboring seven, six, and four distinct mutations, respectively. Mutations such as Leu511Arg+His526Ser and Asp516Val were detected at low rifampicin concentrations (5–10 µg/mL), whereas Ser531Leu mutations dominated at moderate levels (20–80 µg/mL). These findings confirmed that rpoB mutations were influenced by both the region of origin and rifampicin resistance levels, providing critical insights for improving TB diagnosis and optimizing MDR-TB treatment strategies. Keywords: Mycobacterium tuberculosis, rifampicin resistance, rpoB gene mutation
Co-Authors Amalia, Dwi Ayu Lutfiani Andani, Ajeng Putri Retno anti, Monica Widi Ardhini R Maharning Aziz, Saefudin Daniel Joko Wahyono Dini Ryandini Dyah Fitri Kusharyati ELLY PROKLAMASININGSIH Endang Semiarti Fathurrohim, Ramadi Habib Hendro Pramono Isnasari, Azma Nurizqi JAKA WIDADA nurainy, niharoh Nurmalasari, Ade Pratiwi, Meyta Ramadani, Adib Rony, Zahara Tussoleha Slamet Santoso Sodik Wuryanto, Sodik Sri Lestari Stalis Norma Ethica Tri Joko Raharjo Widodo Widodo