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Aryan Morita
Faculty Of Dentistry, Universitas Gadjah Mada, Yogyakarta
Biochemistry, Genetics & Molecular Biology Dentistry Medicine & Pharmacology

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Carbonated hydroxyapatite inflammation’s responses on local rabbits: study of neutrophils’s cell count, macrophages, and edema volumes on mandible Aryan Morita
Biomedical Engineering Vol 1, No 1 (2015): BIOMEDICAL ENGINEERING
Publisher : Biomedical Engineering

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Abstract

Objective: The aims of this study was to find out the response of tissue inflammation implanted CHA thanstandard HA through the amount of neutrophil, macrophage, and edema volumes.Methods. Sixty local rabbits divided into 3 groups that contain 20 rabbits. Each group divided into 5 sub groupsthat observed in day-1, 3, 5, 7, and 14. On first day, all rabbits were anesthesized and incised to create cavity inthe mandible. CHA or HA powder as much as 0,05 g were put into it. Then it was sutured using silk thread. Onthe designated day, the mandibles of 4 rabbits from each sub group were decapitated. The mandibles were fixedwith 10% formalin in PBS pH 7,4. Von Ebner method for decalcification were used followed by hematoxylin eosinstaining to examine neutrophil and macrophage.Results: The number of neutrophil, macrophage, and edema volumes in CHA implantation were lower thanHA. There were not any significant difference (p>0,05) between CHA, HA, and without CHA or HA.Conclusion: There are no differences inflammation respons in CHA implantation and HA.
Carbonated hydroxyapatite inflammation’s responses on local rabbits Aryan Morita; Ngatidjan Ngatidjan; Setyo Purwono
Biomedical Engineering Vol 1, No 1 (2014)
Publisher : Biomedical Engineering

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Objective: This study aimed to find out the response of mandible inflammation implanted with carbonated hydroxyapatite.  Methods: Sixty local rabbits were divided into three groups, including carbonatedhydroxyapatite (CHA) group; hydroxyapatite (HA) group; and control. Each group divided into 5 sub groups for observation in day-1, 3, 5, 7, and 14. On first day, all rabbits were anesthesized and incised to create cavity in the mandible. CHA or HA powder as much as 0,05 g were put into it. Then it was sutured using silk thread. Von Ebner method for decalcification were used followed by hematoxylin eosin staining to examine the neutrophil and macrophage. Results: The number of neutrophil, macrophage, and edema volumes in CHA group were lower than HA group. There were not any significant difference (p>0,05) between CHA, HA, and control.  Conclusion: There are no mandible inflammation response in CHA implantation and HA.Key Words: CHA; edema volumes; inflammation; macrophages; neutrophils
Differences of Streptococcus mutans adhesion between artificial mouth systems: a dinamic and static methods Aryan Morita; H. Dedy Kusuma Yulianto; Susmira Delta Kusdina; Nunuk Purwanti
Dental Journal (Majalah Kedokteran Gigi) Vol. 49 No. 2 (2016): June 2016
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (368.936 KB) | DOI: 10.20473/j.djmkg.v49.i2.p67-70

Abstract

Background: Various materials have been used for treating dental caries. Dental caries is a disease that attacks hard tissues of the teeth. The initial phase of caries is a formation of bacterial biofilm, called as dental plaque. Dental restorative materials are expected for preventing secondary caries formation initiated by dental plaque. Initial bacterial adhesion is assumed to be an important stage of dental plaque formation. Bacteria that recognize the receptor for binding to the pellicle on tooth surface are known as initial bacterial colonies. One of the bacteria that plays a role in the early stage of dental plaque formation is Streptococcus mutans (S. mutans). Artificial mouth system (AMS) used in bacterial biofilm research on the oral cavity provides the real condition of oral cavity and continous and intermittent supply of nutrients for bacteria. Purpose: This study aimed to compare the profile of S. mutans bacterial adhesion as the primary etiologic agent for dental caries between using static method and using artificial mouth system, a dinamic. method (AMS). Method: The study was conducted at Faculty of Dentistry and Integrated Research and testing laboratory (LPPT) in Universitas Gadjah Mada from April to August 2015. Composite resin was used as the subject of this research. Twelve composite resins with a diameter of 5 mm and a width of 2 mm were divided into two groups, namely group using static method and group using dynamic method. Static method was performed by submerging the samples into a 100µl suspension of 1.5 x 108 CFU/ml S. mutans and 200µl BHI broth. Meanwhile AMS method was carried out by placing the samples at the AMS tube drained with 20 drops/minute of bacterial suspension and sterile aquadest. After 72 hours, five samples from each group were calculated for their biofilm mass using 1% crystal violet and read by a spectrofotometer with a wavelength of 570 nm. Meanwhile, one sample from each group was taken for its surface image using Scanning Electron Microscope (SEM). Result: The results showed that S. mutans biofilm mass in the group using static method was 0.34, while in the group using AMS method was 0.09. The results of the statistical analysis then showed that there was a significant difference (p=0.02) in the formation of bacterial biofilm mass between those groups. SEM image in the group using static method also showed that the attachment of S. mutans was more numerous and had a longer chain than in the group using AMS method. Conclusion: There is a difference in the profile of S. mutans bacterial adhesion between using AMS method and static method.
Co-Authors H. Dedy Kusuma Yulianto Ngatidjan Ngatidjan Nunuk Purwanti Setyo Purwono Susmira Delta Kusdina