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Aryan Morita
Faculty Of Dentistry, Universitas Gadjah Mada, Yogyakarta
Biochemistry, Genetics & Molecular Biology Dentistry Medicine & Pharmacology

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Journal : Biomedical Engineering

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Carbonated hydroxyapatite inflammation’s responses on local rabbits: study of neutrophils’s cell count, macrophages, and edema volumes on mandible Aryan Morita
Biomedical Engineering Vol 1, No 1 (2015): BIOMEDICAL ENGINEERING
Publisher : Biomedical Engineering

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Abstract

Objective: The aims of this study was to find out the response of tissue inflammation implanted CHA thanstandard HA through the amount of neutrophil, macrophage, and edema volumes.Methods. Sixty local rabbits divided into 3 groups that contain 20 rabbits. Each group divided into 5 sub groupsthat observed in day-1, 3, 5, 7, and 14. On first day, all rabbits were anesthesized and incised to create cavity inthe mandible. CHA or HA powder as much as 0,05 g were put into it. Then it was sutured using silk thread. Onthe designated day, the mandibles of 4 rabbits from each sub group were decapitated. The mandibles were fixedwith 10% formalin in PBS pH 7,4. Von Ebner method for decalcification were used followed by hematoxylin eosinstaining to examine neutrophil and macrophage.Results: The number of neutrophil, macrophage, and edema volumes in CHA implantation were lower thanHA. There were not any significant difference (p>0,05) between CHA, HA, and without CHA or HA.Conclusion: There are no differences inflammation respons in CHA implantation and HA.
Carbonated hydroxyapatite inflammation’s responses on local rabbits Aryan Morita; Ngatidjan Ngatidjan; Setyo Purwono
Biomedical Engineering Vol 1, No 1 (2014)
Publisher : Biomedical Engineering

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Abstract

Objective: This study aimed to find out the response of mandible inflammation implanted with carbonated hydroxyapatite.  Methods: Sixty local rabbits were divided into three groups, including carbonatedhydroxyapatite (CHA) group; hydroxyapatite (HA) group; and control. Each group divided into 5 sub groups for observation in day-1, 3, 5, 7, and 14. On first day, all rabbits were anesthesized and incised to create cavity in the mandible. CHA or HA powder as much as 0,05 g were put into it. Then it was sutured using silk thread. Von Ebner method for decalcification were used followed by hematoxylin eosin staining to examine the neutrophil and macrophage. Results: The number of neutrophil, macrophage, and edema volumes in CHA group were lower than HA group. There were not any significant difference (p>0,05) between CHA, HA, and control.  Conclusion: There are no mandible inflammation response in CHA implantation and HA.Key Words: CHA; edema volumes; inflammation; macrophages; neutrophils
Co-Authors H. Dedy Kusuma Yulianto Ngatidjan Ngatidjan Nunuk Purwanti Setyo Purwono Susmira Delta Kusdina