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All Journal Indonesian Journal of Chemistry and Environment
Choirul Chasanah
Universitas Negeri Yogyakarta
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Materials Science & Nanotechnology

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The Influence of Al3+ Metal Ion in AlCl3 Compound On α- Amylase Enzyme Activity Choirul Chasanah; Das Salirawati
Indonesian Journal of Chemistry and Environment Vol 3, No 2 (2020): DECEMBER 2020
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (493.934 KB) | DOI: 10.21831/ijce.v3i2.43512

Abstract

The influence of  Al3+ metal ion in  AlCl3 towards α-amylase enzyme activity have been studied. The activity of the α-amylase enzyme on potato starch substrate was determined using the DNS method (3,5-dinitrosalicylic acid) at optimum conditions. The data analysis was conducted in a descriptive qualitative manner. The results obtained that α-amylase enzyme activity with potato starch substrate was optimum at  20 mg/mL with pH 7.2, incubation temperature 37˚C, incubation time 15 minutes, and enzyme concentration of 40 mg/mL with an average enzyme activity value of 0.0079 mg/mL/minute at 37˚C. However, after the addition of Al3+ metal ion, the average value of enzyme activity decreased. These means that empirically Al3+ metal ion has a tendency to be inhibitory to the α-amylase enzyme activity on potato starch substrates.
Co-Authors Das Salirawati