Tri Rahayuningsih
Merdeka University Ponorogo

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ISOLASI DAN SELEKSI BAKTERI SEBAGAI AGEN BIOREMEDIASI LIMBAH CAIR PABRIK KAYU PUTIH Tri Rahayuningsih; Umi Isnatin; Parwi Parwi
JURNAL AGRI-TEK : Jurnal Penelitian Ilmu-Ilmu Eksakta Vol. 19 No. 2 (2018): AGRI-TEK : Jurnal Penelitian Ilmu Ilmu Eksakta
Publisher : Universitas Merdeka Madiun

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (282.86 KB) | DOI: 10.33319/agtek.v19i2.15

Abstract

This study aims to isolate and select bacteria as a bioremediation agent for wastewater of cajeput factory. The waterwaste of cajuput factory has not been utilized for liquid organic fertilizer because it still contains cajeput oil (phenol compound) which can disrupt plant growth. Therefore it is necessary to look for bacteria that have the ability to integrate of management waterwaste. Waterwaste samples is taken by random sampling in tri locations, namely the initial shelter, the second shelter and the waste disposal area. Bacteria are developed in liquid media, then isolated and identified on solid media. Identification of bacteria based on colony morphology, cell morphology and biochemical testing. Phenol degradation test was carried out using a completely randomized design with tri replications. Treatment of types of bacterial isolates namely B0 = Control, B1 = Bacillus, B2 = Psedomonas, B3 = Flavobacterium. The results showed that there were tri bacterial isolates found in wastewater of cajuput factory namely Bacillus sp, Pseudomonas sp and Flavobacterium sp. The highest amount of bacterial colonies was found in waste shelters of 2.41 x 106 colonies. The most effective bacteria in degrading phenol is Pseudomonas sp
Pemanfaatan Limbah Kolam Lele dan Bacillus sp untuk meningkatkan pertumbuhan dan hasil Padi (Oryza sativa) Tri Rahayuningsih
JURNAL AGRI-TEK : Jurnal Penelitian Ilmu-Ilmu Eksakta Vol. 21 No. 2 (2020): JURNAL AGRITEK
Publisher : Universitas Merdeka Madiun

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (459.883 KB) | DOI: 10.33319/agtek.v21i2.62

Abstract

Abstract— Catfish pond waste has a high content of organic matter and nutrients (nitrogen) which can be used as a source of nutrition for rice plants, but on the other hand it also contains ammonia which can interfere with rice growth. Therefore, research is needed to reduce the negative effects of using catfish pond waste. in rice cultivation, by utilizing Bacillus sp. The purpose of this research is to obtain information about how to use catfish pond waste for rice cultivation, by utilizing the Bacillus sp. Bacteria. Bacillus sp is thought to increase nutrient availability for rice. The method to be carried out in this study used a randomized block design with treatment A0 (control), A1 (Bacillus sp), A2 (catfish waste), A3 (catfish waste + Bacillus sp), A4 (Urea fertilizer). The results of this study found that pond waste and Bacillus sp. Affected the growth and yield of rice. The highest results were obtained in the combination treatment of pond waste with Bacillus sp.  Keywords—: Bacillus sp; catfish pond waste; organic rice.