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All Journal WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences Jurnal Ternak Tropika
T Susilawati
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Veterinary

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Factors Affecting Spermatozoa Motility Analysis using CASA Dian Ratnawati; N Isnaini; T Susilawati
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 29, No 3 (2019): September 2019
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (147.715 KB) | DOI: 10.14334/wartazoa.v29i3.2012

Abstract

Motility is a very important parameter to determine the quality of semen. Spermatozoa motility assessment can be done manually (subjectively) or with CASA. The superiority of motility assessment using CASA compared to manually is more objective, accurate, fast, efficient and able to provide detailed motility of spermatozoa. However, in implementation, assessments with CASA produce varied data. So far, there has not been a spermatozoa motility standard with CASA, except by minimizing variations in the factors that influence the results of analysis with CASA. The purpose of this paper is to describe factors that affecting spermatozoa motility analysis using CASA to reach optimal motility analysis. Some influencing factors include: CASA settings, semen diluent, spermatozoa concentration, chamber, analyst, sample preparation and analysis time. Recommendation standard motility assessments using CASA include: experienced and consistent analysts, sample preparation properly (mixing, pipeting and sampling), spermatozoa concentration of 20 million / ml, consistent in chamber type (conditioned 37°C), semen diluents and CASA settings (frame rate of 60 Hz and frame per field 30) and fast implementation of analysis (
EFEKTIFITAS PENAMBAHAN HORMON GONADOTHROPIN PADA MEDIUM MATURASI mSOF TERHADAP TINGKAT MATURASI OOSIT G Ciptadi; T Susilawati; B Siswanto; Helli N. Karima
TERNAK TROPIKA Journal of Tropical Animal Production Vol 12, No 1 (2011): Ternak Tropika
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (79.095 KB)

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ABSTRAK Tujuan penelitian ini adalah untuk mengetahui efektifitas supplementasi hormon gonadotrophin pada medium kultur maturasi sel terhadap tingkat pematangan oosit kambing secara in vitro. Materi yang digunakan adalah oosit kambing immature, FBS (GIBCO), NaCL Fisiologis 0,9%, mSOF, FSH, LH, hCG,PMSG (Intervet) Streptomicyn, Penicillin (Meiji),. Oosit kambing diperoleh dengan aspirasi ovarium kambing dari RPH Sukun – Malang. Ovarium dibawa dalam termos suhu 38 0C, diaspirasi folikel berdiameter 2 – 6 mm. Evaluasi didasarkan expansi cumullus oophorus dan keberadaan first polar body. Percobaan menggunakan Rancangan Acak Lengkap dan dilanjutkan dengan uji BNT, pada masing-masing perlakuan penambahan hormon .Hasil menunjukkan bahwa tingkat maturasi oosit kambing berdasarkan pengamatan ekspansi cumullus oophorus adalah 54% (FSH + LH) dan 44% (PMSG + hCG). Tidak ada pengaruh penambahan hormon yang berbeda terhadap tingkat maturasi. Tingkat maturasi berdasarkan first polar body adalah 64,5% (FSH + LH) dan 68 % (PMSG + hCG). Hasil analisa statistika tidak menunjukkan pengaruh penambahan hormon yang berbeda. Kesimpulan penelitian ini adalah penambahan hormon FSH + LH dan  PMSG + hCG tidak berbeda nyata. Disarankan menambahkan hormon PMSG + hCG untuk maturasi in vitro pada medium mSOF karena harga hormon tersebut lebih murah dan perlu dilakukan mengenai ukuran penambahan dosis hormon yang sesuai. Kata Kunci: medium m SOF, IVM, Gonadotrophin, Oosit, Kambing   THE EFFICIENCY OF GONADOTROPHINB SUPPLEMENTATION OF MSOF MEDIUM ON THE MATURATION RATE OF IMMATURE OOCYTE IN VITRO.   Abstract The aims of the research is to stydy effectifity of gonadothrophin supplementation on maturation rate of immmature oocyte of goat in vitro. Immature oocyte was isolated from 2-6 mm diameter of folicles. Medium stock was suplemented with different treatment of Gonadotrophin : mSOF, FSH, LH, hCG (Intervet), PMSG (Intervet) Streptomicyn (Meiji), Penicillin (Meiji), Maturation rate was evaluated base on the cumulus oocyte  expantion and fisrt polar body extruction.  Result showed that there are no significant effect of hormomal suplementation on mSOF medium, wich  cumulus expantion of  54% (FSH + LH) dan 44% (PMSG + hCG). Meanwhilebase on extruction of first polar body was 64,5% (FSH + LH) dan 68 % (PMSG + hCG). It was conluded that hormonal treatment resulted in not diffetent effect to maturation rate . Suggested to, for practical purposes, using   PMSG + hCG  for IVM for the reason of in expensive and feasibility of hormone.   Key words: medium m SOF, IVM, Gonadotrophin, Oosit, Goat
Co-Authors B Siswanto Dian Ratnawati G Ciptadi Helli N. Karima N Isnaini