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Lipid Peroxidation Level on Moderate-Intensity Interval Exercise on Non-Athlete Adolescent Sugiharto; Desiana Merawati; Olivia Andiana; Adi Pranoto
Kinestetik : Jurnal Ilmiah Pendidikan Jasmani Vol 6 No 4 (2022): DESEMBER (ACCREDITED SINTA 3)
Publisher : UNIB Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/jk.v6i4.22867

Abstract

Exercise is commonly perceived as a pleasurable and healthy activity. However, it is classified as a complex activity that potentially enhances the formation of free radicals and oxidative stress. This study aims to analyze lipid peroxidation, as the biomarker of free radicals, in interval exercise. A total of 16 trained non-athlete male adolescents, aged 20-21 years old, with normal blood pressure, normal resting heart rate, and gave good level of physical fitness voluntarily participated in this study. The participants were randomly separated into two groups, namely the K1 (n=8, interval exercise with active resting medium intensity pedaling ergometer bike) and  K2 (n=8, interval exercise with resting without pedaling the ergometer bike). The interval exercise was carried out at 60-70% HRmax intensity for 35 minutes. The blood sample for Malondialdehyde (MDA) level analysis, as the lipid peroxidation biomarker, was taken using Thiobarbituric acid reactive substances (TBARS). The blood samples were collected before and after the interval exercise.  The obtained data were analyzed using the one-way ANOVA test with a 5% significance level. Our results suggest no significant different level of MDA, a lipid peroxidation biomarker, between the interval exercise with resting through medium intensity pedaling ergometer bike and the interval exercise with resting with no pedaling ergometer bike. However, the interval exercise resting through pedaling an ergometer bike presented relatively higher lipid peroxidation than the interval exercise resting without pedaling an ergometer bike. Therefore, following the free radical concept, interval exercise with resting through pedaling an ergometer bike is more beneficial than the exercise without pedaling an ergometer bike resting.
Moderate Intensity Training By Listening To Music Decreases Interleukin-6 Levels In Rats Septyaningrum Putri Purwoto; Adi Pranoto; Hartono Hartono; Sugiharto Sugiharto
STRADA : Jurnal Ilmiah Kesehatan Vol. 9 No. 1 (2020): May
Publisher : Universitas STRADA Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30994/sjik.v9i1.272

Abstract

The design of this study is the randomized control group posttest-only design, 21 of Rattus Norvegicus Strain Wistar male, 20-24 weeks, 250 ± 350 grams and randomly divided into 3 groups namely CONT (n = 7, control without treatment), MIT (n = 7, moderate intensity training, treadmill with speed 14-16 m/min for 15-30 minutes listening to music tempo 160 beats/min) and HIT (n = 7, high intensity training, treadmill with speed 22-25 m/min for 15-30 minutes listening to music tempo 160 beats/min). The intervention was started from 17.00-20.00 WIB with a frequency of 3 times / week for 10 weeks. Blood is drawn for 12 hours after the last training. Measurement of IL-6 serum levels using the ELISA method. Data analysis techniques using ANOVA test and LSD post hoc test with the Statistical Package for Social Science (SPSS).