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Efek Histopotogenik dan Daya Tolak Ekstrak Biji Mengkudu (Morinda Citrifolia) Terhadap Vektor Demam Berdarah Adhi Kumoro Setya; Tri Harningsih - Sekolah Tinggi Ilmu Kesehatan Nasional
Indonesian Journal on Medical Science Vol 6 No 1 (2019): IJMS 2019
Publisher : Unit Penelitian dan Pengabdian Masyarakat Politeknik Kesehatan Bhakti Mulial

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (644.303 KB)

Abstract

Abstract: Aedes aegypti mosquitoes are categorized as dangerous insects since they often transmit deadly diseases to humans, such as dengue fever. In the current decades, the position of chemical insecticides has caused resistance of vector and reduced environmental quality due to the residue produced. This research aims to test the repellent and killing power on larvae (larvicide) of ethanol extract of noni seeds as botanical insecticide. Repellent power of the extract was analyzed using anova test. Larvicide power was tested using probit analysis and its pathological effect was examined histologically. This research used 7 (seven) variations of concentration with repetitive test for 3 (three) times. Based on the anova test, noni seeds were not effective as a repellent of mosquitoes. Meanwhile, based on probit analysis, it was shown that lethal concentration (LC50) happened in concentration of 0.03% for instar larvae 1; 0.04% for instar larvae 2; and 0.07% for instar larvae 3. Significant result of one way anova > 0,05 while histopathology description shows that larval intestinal epithelium undergoes vacuolization, and the cuticle tissue did not show any significant damage. Based on the research result, it can be reported that ethanol extract of noni seeds is not effective as a repellent, but it has a good larvicidal effect, being close to positive control which gives pathological effect on the intestinal tissue.Key words: Aedes aegypti, Mengkudu (Morinda citrifolia), Extracts, larvicide, Repellent Abstrak: Nyamuk Aedes aegypti masuk dalam kategori serangga berbahaya karena seringnya menularkan penyakit mematikan kepada manusia contohnya demam berdarah. Dalam beberapa dekade, posisi insektisida kimia telah menyebabkan resistensi dari vektor dan menurunnya kualitas lingkungan akibat residu yang dihasilkan. Penelitian ini bertujuan untuk menguji kemampuan daya tolak dan daya bunuh terhadap larva (larvasida) dari ekstrak etanol biji mengkudu sebagai insektisida botani. Daya tolak ekstrak dianalisa menggunakan  uji anova. Kemampuan larvasida dilakukan menggunakan analisis probit dan efek patologisnya dibaca secara histologi. Penelitian ini menggunakan 7 (tujuh) variasi konsentrasi dengan penggulangan uji sebanyak 3 (tiga) kali. Dari hasil uji anova menunjukan biji mengkudu tidak efektif sebagai repellent (penolak) nyamuk sedangkan dari analisis probit menunjukan lethal concentration (LC50) terjadi pada konsentrasi 0,03% untuk larva instar 1; 0,04% untuk larva instar 2 dan 0,07% untuk larva instar 3. Nilai signifikasi uji one way anova > 0,05 sedangkan gambaran histopatologi  menunjukan epitel usus larva mengalami vakuolisasi sedangkan jaringan kutikula tidak memperlihatkan kerusakan berarti. Dari hasil penelitian ini dapat  dilaporkan bahwa ekstrak etanol biji mengkudu tidak efektif sebagai repellent (penolak) tetapi mempunyai daya larvasida yang bagus mendekati kontrol positif yang berefek patologis pada jaringan ususnya.Kata kunci: Aedes aegypti, Morinda citrifolia, Ekstrak, Larvasida, Daya tolak
Prevalensi Trikomoniasis pada Wanita Risiko Tinggi di Kawasan Wisata Baturraden Kabupaten Banyumas Sri Widyastuti; Adhi Kumoro Setya
Indonesian Journal on Medical Science Vol 10 No 1 (2023): IJMS 2023
Publisher : Unit Penelitian dan Pengabdian Masyarakat Politeknik Kesehatan Bhakti Mulial

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55181/ijms.v10i1.407

Abstract

Trichomoniasis is a sexually transmitted disease with a high incidence. People with multiple sexual partners are believed to be the main transmission factors. In recent years, the number of sexually transmitted infections in Banyumas has been quite high. The existence of Gang Sadar which is the residence of women sex workers, with a strategic location and close to tourist attractions in Baturraden, makes many people visit. These conditions increase the risk of transmission of trichomoniasis in the region. The purpose of this study was to find out the number of cases of trichomoniasis on urine samples of high-risk women in the Baturraden tourist area. This research descriptive in nature by involving female research respondents with high risk job. In each research respondent an examination was carried out from the urine specimen and as a support for the collection of respondent's life background. The data is presented in tables and calculated percentage of infected respondentsTrichomonas vaginalis. From the inspection results laboratory found parasitesTrichomonas vaginalis as many as 2 people from30 research respondents. Based on sociodemographic data, case finding occurredon respondents with characteristics; over 21 years old, last education Middle and high school, karaoke guide profession and prostitutes and marital status have not married and divorced. From the data on the sexual activity of the respondents, came up with characteristics; all showed symptoms of abnormal vaginal discharge and odor as wellserving 1-3 customers per day. The results of this study obtained conclusions that trichomoniasis cases were 6.7% with heterogeneous characteristics of respondents.