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Efek Histopotogenik dan Daya Tolak Ekstrak Biji Mengkudu (Morinda Citrifolia) Terhadap Vektor Demam Berdarah Adhi Kumoro Setya; Tri Harningsih - Sekolah Tinggi Ilmu Kesehatan Nasional
Indonesian Journal on Medical Science Vol 6 No 1 (2019): IJMS 2019
Publisher : Unit Penelitian dan Pengabdian Masyarakat Politeknik Kesehatan Bhakti Mulial

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (644.303 KB)

Abstract

Abstract: Aedes aegypti mosquitoes are categorized as dangerous insects since they often transmit deadly diseases to humans, such as dengue fever. In the current decades, the position of chemical insecticides has caused resistance of vector and reduced environmental quality due to the residue produced. This research aims to test the repellent and killing power on larvae (larvicide) of ethanol extract of noni seeds as botanical insecticide. Repellent power of the extract was analyzed using anova test. Larvicide power was tested using probit analysis and its pathological effect was examined histologically. This research used 7 (seven) variations of concentration with repetitive test for 3 (three) times. Based on the anova test, noni seeds were not effective as a repellent of mosquitoes. Meanwhile, based on probit analysis, it was shown that lethal concentration (LC50) happened in concentration of 0.03% for instar larvae 1; 0.04% for instar larvae 2; and 0.07% for instar larvae 3. Significant result of one way anova > 0,05 while histopathology description shows that larval intestinal epithelium undergoes vacuolization, and the cuticle tissue did not show any significant damage. Based on the research result, it can be reported that ethanol extract of noni seeds is not effective as a repellent, but it has a good larvicidal effect, being close to positive control which gives pathological effect on the intestinal tissue.Key words: Aedes aegypti, Mengkudu (Morinda citrifolia), Extracts, larvicide, Repellent Abstrak: Nyamuk Aedes aegypti masuk dalam kategori serangga berbahaya karena seringnya menularkan penyakit mematikan kepada manusia contohnya demam berdarah. Dalam beberapa dekade, posisi insektisida kimia telah menyebabkan resistensi dari vektor dan menurunnya kualitas lingkungan akibat residu yang dihasilkan. Penelitian ini bertujuan untuk menguji kemampuan daya tolak dan daya bunuh terhadap larva (larvasida) dari ekstrak etanol biji mengkudu sebagai insektisida botani. Daya tolak ekstrak dianalisa menggunakan  uji anova. Kemampuan larvasida dilakukan menggunakan analisis probit dan efek patologisnya dibaca secara histologi. Penelitian ini menggunakan 7 (tujuh) variasi konsentrasi dengan penggulangan uji sebanyak 3 (tiga) kali. Dari hasil uji anova menunjukan biji mengkudu tidak efektif sebagai repellent (penolak) nyamuk sedangkan dari analisis probit menunjukan lethal concentration (LC50) terjadi pada konsentrasi 0,03% untuk larva instar 1; 0,04% untuk larva instar 2 dan 0,07% untuk larva instar 3. Nilai signifikasi uji one way anova > 0,05 sedangkan gambaran histopatologi  menunjukan epitel usus larva mengalami vakuolisasi sedangkan jaringan kutikula tidak memperlihatkan kerusakan berarti. Dari hasil penelitian ini dapat  dilaporkan bahwa ekstrak etanol biji mengkudu tidak efektif sebagai repellent (penolak) tetapi mempunyai daya larvasida yang bagus mendekati kontrol positif yang berefek patologis pada jaringan ususnya.Kata kunci: Aedes aegypti, Morinda citrifolia, Ekstrak, Larvasida, Daya tolak
Prevalensi Trikomoniasis pada Wanita Risiko Tinggi di Kawasan Wisata Baturraden Kabupaten Banyumas Sri Widyastuti; Adhi Kumoro Setya
Indonesian Journal on Medical Science Vol 10 No 1 (2023): IJMS 2023
Publisher : Unit Penelitian dan Pengabdian Masyarakat Politeknik Kesehatan Bhakti Mulial

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55181/ijms.v10i1.407

Abstract

Trichomoniasis is a sexually transmitted disease with a high incidence. People with multiple sexual partners are believed to be the main transmission factors. In recent years, the number of sexually transmitted infections in Banyumas has been quite high. The existence of Gang Sadar which is the residence of women sex workers, with a strategic location and close to tourist attractions in Baturraden, makes many people visit. These conditions increase the risk of transmission of trichomoniasis in the region. The purpose of this study was to find out the number of cases of trichomoniasis on urine samples of high-risk women in the Baturraden tourist area. This research descriptive in nature by involving female research respondents with high risk job. In each research respondent an examination was carried out from the urine specimen and as a support for the collection of respondent's life background. The data is presented in tables and calculated percentage of infected respondentsTrichomonas vaginalis. From the inspection results laboratory found parasitesTrichomonas vaginalis as many as 2 people from30 research respondents. Based on sociodemographic data, case finding occurredon respondents with characteristics; over 21 years old, last education Middle and high school, karaoke guide profession and prostitutes and marital status have not married and divorced. From the data on the sexual activity of the respondents, came up with characteristics; all showed symptoms of abnormal vaginal discharge and odor as wellserving 1-3 customers per day. The results of this study obtained conclusions that trichomoniasis cases were 6.7% with heterogeneous characteristics of respondents.
INFEKSI SOIL TRANSMITTED HELMINTS PADA PETERNAK SAPI Dwi Haryatmi; Adhi Kumoro Setya; Fitria Diniah Jannah Sayekti; A.Dicha Putri Maharani
Klinikal Sains : Jurnal Analis Kesehatan Vol. 13 No. 2 (2025): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v13i2.7004

Abstract

Soil Transmitted Helminths (STH) infection is a worm whose life cycle requires suitable soil to reproduce into an infective form. One of the jobs related to land is cattle raising. The purpose of this study was to determine the presence of STH eggs in the nails and faeces of cattle breeders in Kedawung Village, Jumapolo District, Karanganyar Regency. This study used descriptive research using concentrated salt flotation method. Research data is presented in tabular form and expressed in percent. The conclusion showed that 2 (13.3%) out of 15 cattle breeder respondents in Pamotan Hamlet RT 12/06 Kedawung Village, Jumapolo District, Karanganyar Regency were infected with STH which was indicated by the finding of hookworm eggs in the stool samples and negative results in the samples nail.
Prevalence of Trichomoniasis in Women with HIV in Sragen District Hanny Endah Setiowati; Adhi Kumoro setya
Indonesian Journal of Global Health Research Vol 7 No 3 (2025): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v7i3.5954

Abstract

Trichomonas vaginalis infection remains one of the most common non-viral sexually transmitted infections (STIs) globally, despite being one of the most easily treatable STIs. This study aims to determine the prevalence and risk factors associated with trichomoniasis among HIV-positive women in Sragen Regency. A total of 30 HIV-diagnosed women from the Sehat Panghuripan Sukowati Foundation in Sragen, aged 15–49 years, participated in this study. Respondents completed a questionnaire and underwent urinalysis at Prodia Medical Laboratory, Sragen. The presence of Trichomonas vaginalis infection was evaluated using a wet mount microscopic method. Clinical features were collected through structured questionnaires administered to each participant. Result: The findings revealed the prevalence of Trichomoniasis among HIV-positive women, indicating that Trichomoniasis can increase HIV viral load in vaginal fluid, potentially heightening the risk of HIV transmission. The study demonstrated a higher infection rate in women with a history of sexually transmitted infections (HIV), particularly those over 30 years of age. Most participants were housewives with a single sexual partner, reported no vaginal discharge complaints, but had experienced abnormal vaginal secretions in the past. The recorded prevalence of Trichomoniasis in this study was 10% (3 out of 30 samples)
Optimisation of 3% Giemsa Staining Time in Malaria Microscopic Examination Resa Cristina Lilik Hadiana; Adhi Kumoro Setya
Indonesian Journal of Global Health Research Vol 7 No 3 (2025): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v7i3.6229

Abstract

Malaria infection is a significant health issue in Indonesia, with a high prevalence in endemic areas such as Puskesmas Hanura Teluk Pandan. Giemsa 3% staining is used as the standard method for microscopic diagnosis of malaria; however, the duration of staining can affect laboratory efficiency. This study aims to optimize the staining time of Giemsa 3% in microscopic malaria diagnosis to improve efficiency without compromising diagnostic quality. The study used an experimental design with staining time variations of 30 minutes, 40 minutes, and 50 minutes on thin blood smear samples from suspected malaria patients. Staining quality was assessed using a scoring system, with a score of 1 indicating optimal staining (clear background, blue cytoplasm, and red nucleus) and a score of 0 indicating suboptimal staining (unclear background, cytoplasm, and nucleus not stained or not clearly visible). The results showed that all staining time variations produced optimal staining quality (score 1), with no samples receiving a score of 0. The data were found to be non-homogeneous and non-normally distributed, thus the Kruskal-Wallis test was employed for subsequent analysis. The Kruskal-Wallis test showed no significant differences between the three staining time variations (p = 1.000). The conclusion of this study is that staining durations of 30 minutes, 40 minutes, and 50 minutes with Giemsa 3% can produce equivalent staining quality, allowing operational efficiency without compromising the accuracy of malaria diagnosis.