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All Journal The Journal of Experimental Life Sciences (JELS) Research Journal of Life Science AGRIVITA, Journal of Agricultural Science
Aminuddin Affandi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience

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ISOLATION AND PHENOTYPIC CHARACTERISATION OF MORPHOLOGY IN FUNGUS Beauveria bassiana (Balsamo) Vuillemin COLONY NATURALLY FROM LEAF SURFACE, SOIL, AND INSECT AS HOST IN TOMATO PLANTATION Affandi, Aminuddin; Chailani, Siti Rasminah; Mimbar, Saubari M.; Wiroatmodjo, Boedijono
AGRIVITA, Journal of Agricultural Science Vol 34, No 3 (2012)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Virulence of fungus Beauveria bassiana as pathogen on insect can be predicted according to phenotypic character in colony morphology. First of all, isolates of three different habitats were taken. Furthermore, their phenotypic characters was further characterised in B. bassiana colony and the colony growth rate was also monitored. The three natural habitats comprised insects, soil in tomato plantation and the surface of tomato leaves. The result showed that B bassiana isolate managed to be isolated from the three natural habitats involving larva Spodoptera litura and Helicoverpa armigera, the soil, and surface of tomato leaves. B bassiana isolate of insect habitat represented phenotype in colony morphology with curved character, while isolate of the leaves’ surface and soil indicated phenotype in colony morphology with raised and bowl-shaped character. In addition to the colony characters, varied growth rate was also indicated by isolate from each habitat. Isolate of insect with curved colony character represented higher growth rate than that of the leaves’ surface or the soil with raised and bowl-shaped colony character. The colony with curved character represented phenotypic character of isolate B bassiana with high growth rate. Thus, B. bassiana isolate with the curved colony of insect served as an isolate which was expected to be virulent as controlling agent to biological elements.       Keywords: B. bassiana isolate, phenotypic characters in colony morphology, colony growth rate, habitat of fungus
ISOLATION AND PHENOTYPIC CHARACTERISATION OF MORPHOLOGY IN FUNGUS Beauveria bassiana (Balsamo) Vuillemin COLONY NATURALLY FROM LEAF SURFACE, SOIL, AND INSECT AS HOST IN TOMATO PLANTATION Aminuddin Affandi; Siti Rasminah Chailani; Saubari M. Mimbar; Boedijono Wiroatmodjo
AGRIVITA, Journal of Agricultural Science Vol 34, No 3 (2012)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v34i3.229

Abstract

Virulence of fungus Beauveria bassiana as pathogen on insect can be predicted according to phenotypic character in colony morphology. First of all, isolates of three different habitats were taken. Furthermore, their phenotypic characters was further characterised in B. bassiana colony and the colony growth rate was also monitored. The three natural habitats comprised insects, soil in tomato plantation and the surface of tomato leaves. The result showed that B bassiana isolate managed to be isolated from the three natural habitats involving larva Spodoptera litura and Helicoverpa armigera, the soil, and surface of tomato leaves. B bassiana isolate of insect habitat represented phenotype in colony morphology with curved character, while isolate of the leaves’ surface and soil indicated phenotype in colony morphology with raised and bowl-shaped character. In addition to the colony characters, varied growth rate was also indicated by isolate from each habitat. Isolate of insect with curved colony character represented higher growth rate than that of the leaves’ surface or the soil with raised and bowl-shaped colony character. The colony with curved character represented phenotypic character of isolate B bassiana with high growth rate. Thus, B. bassiana isolate with the curved colony of insect served as an isolate which was expected to be virulent as controlling agent to biological elements.       Keywords: B. bassiana isolate, phenotypic characters in colony morphology, colony growth rate, habitat of fungus
Population Dynamic and Distribution of Bactrocera carambolae and Bactrocera dorsalis in Orchard Habitat in Different Geographical Areas Kolopaking, Budiarto; Rizali, Akhmad; Affandi, Aminuddin; Hudiwaku, Syarron; Himawan, Toto
Research Journal of Life Science Vol 10, No 1 (2023)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2023.010.01.1

Abstract

Fruitflies, particularly Bactrocera carambolae and Bactrocera dorsalis, are significant pests that have the potential to diminish the quality and quantity of agricultural products. They may live in diverse habitats, particularly in agroecosystem habitats. Orchard habitats offer a source of food and development for fruit flies like B. carambolae and B. dorsalis not only in Java, notably Majalengka Regency, but also in Lombok Island and West Nusa Tenggara. Consequently, the purpose of there search is to observe (1) The population dynamic of B. carambolae and B. dorsalis in the orchard habitat. (2) Distribution of fruit flies B. carambolae and B. dorsalis trapping results in the orchard habitat. (3) Analyzing the factors affecting the presence of the fruit fly species B. carambolae and B. dorsalis in the orchard habitat. Fruitflies were sampled using the trap method between December 2021 and March 2022. In the orchard habitat on the mangosteen fruit commodity, the results indicated that B. carambolae and B. dorsalis had the largest population densities. Additionally, orchard locations on Lombok Island had the highest population density. Furthermore, fruitflies are more prevalent in perennial crops than in annual ones due to higher population density. Perennial crops are the ideal conditions for fruit fly development as compared to seasonal crops.
Phylogenetic Patterns of Genus Megophrys (Anura: Megophryidae) from Java and Sumatra Kurniawan, Nia; Affandi, Aminuddin; Wardani, Erintha Eka; Chomsy, Indah Nur; Firdaus, Anggun Sausan; Kurnianto, Agung Sih
The Journal of Experimental Life Science Vol. 7 No. 2 (2017)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1128.003 KB) | DOI: 10.21776/ub.jels.2017.007.02.03

Abstract

The emergence of Java and Sumatra islands at the end of Miocene, has impact to the diversity of fauna that living in the islands. Amphibia, as one of a genetic information series, can be used as a good phylogenetic comparison. Genus Megophrys (Anura: Megophryidae), is a member of amphibians that live isolated at an altitude of 1000 ASL (Above Sea Level) or more. The purpose of this research is to analyze the phylogenetic patterns of genus Megophrys in Java and Sumatra based on the analysis of 16s rRNA gene. We also analyzed the zoogeography to understand the history of relationship and the establishment of these islands. We took samples on 12 localities: 10 areas of Sumatra and 2 areas of Java. We used Qiamp Blood and Tissue Kit to extract the DNA from tissues. Qualitative analysis of DNA was done by running electrophoresis on 1% agarose gel. Qualitative analysis of DNA was done by running electrophoresis on 1% agarose gel. Results show that Clade A has 2 subclasses: I and II. Subclade I consists of Sumatran specimens, whereas subclade II consists of Javanese specimens. Subclaude I and II have paraphyletic connections. Clade B classified as politomy clade consists of Megophrys sp. from West Pasaman, Megophrys sp. from West Sumatra and Megophrys sp. from Pasawaran, Lampung. The result shows that Megophrys sp. of subclade I (clade A) and clade B, belongs to the same group, both of them were originated from Sumatra. Megophrys sp. subclade I (clade A) originated from Tanggamus (Lampung) and West Lampung (Lampung) separated with 2 species of Megophrys sp. of clade B originated from Pasawaran (Lampung).Keywords: 16s rRNA, Java, Megohprys, Sumatra, zoogeography
Co-Authors Agung Sih Kurnianto Akhmad Rizali Boedijono Wiroatmodjo Chomsy, Indah Nur Firdaus, Anggun Sausan Hudiwaku, Syarron Kolopaking, Budiarto Nia Kurniawan Saubari M. Mimbar Siti Rasminah Chailani Toto Himawan Wardani, Erintha Eka