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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) AGRIVITA, Journal of Agricultural Science
Rosa Yunita
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Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance

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Adaptability of Mutant Genotypes of Artemisia (Artemisia annua L.) as Result Of Gamma Irradiation in Three Locations with Different Altitude Syukur, Muhamad; Lestari, Endang Gati; Purnamaningsih, Ragapadmi; Yunita, Rosa; Aisyah, Syarifah Iis; Firdaus, Rohim
AGRIVITA, Journal of Agricultural Science Vol 33, No 3 (2011)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

The objective of this study was to identify the adaptability of twelve artemisia mutant genotypes, which were planted in three locations with different altitude, as a result of gamma irradiation. Randomized Complete Block Design (RCBD) was applied in this research with three replications as blocks. The genotypes 1B, 1C, 1D, 2, 3, 4, 5A, 6B, 7A, 8, 14, 15 and two control genotypes as parent genotype from seed and from in vitro were used. The genotypes were planted in three different locations such as Mount Putri, Cianjur (1450 m above sea level), Pacet, Cianjur (950 m above sea level) and Cicurug, Sukabumi (540 m above sea level). Based on the method of postdictive and predictive success, the model used was AMMI2 which was able to explain up to 100% of interaction-influenced variation. The genotypes which were found stabile and adaptive in these three locations were 1B, 1C, 1D, 6B and 15. Genotypes 3 and 7A were adaptive specifically in Pacet area, 5A was adaptive for Gunung Putri while genotype 4 was for Cicurug only.Keywords: AMMI, Artemisia annua, mutant genotype, adaptability
Induksi Kalus dan Regenerasi Tunas Padi Varietas Fatmawati Endang G Lestari; Rosa Yunita
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 36 No. 2 (2008): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (39.121 KB) | DOI: 10.24831/jai.v36i2.20479

Abstract

Efficiency of shoot regeneration from rice callus explant is an important factor, particularly for the purpose of plant genetic improvement such as somaclonal variation, transformation and in vitro selection. In the attempt of gaining shoot regeneration, callus induction and shoot regeneration experiments were conducted in the Biological Cell and Tissue Culture Laboratory, Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development. Mature seed was used as an explant. The media formulation for calli induction was MS + 2.4-D (1, 3, 5 and 7 mg/l ) + prolin (0 and 100 mg/l ) and hydrolyzed casein 3000 mg/l. The media for shoot regeneration was (1) MS + BA (1.2 and 3 mg/l ) + IAA 0.8 mg/l + prolin 100 mg/l, and (2) MS + BA 2 mg/l + IAA 0.8 mg/l + zeatin (0.1; 0.2 and 0.3 mg/l). The result showed that the embryogenic calli could be produced from MS + 2.4-D 3 mg/l + hydrolyzed casein 3000 mg/l treatment and the best media for shoot induction was MS + BA 2 mg/l + IAA 0.8 mg/l + zeatin 0.2 mg/l. The obtained plantlets were successfully acclimatized in the greenhouse.
Co-Authors Endang G Lestari Endang Gati Lestari Muhamad Syukur RAGAPADMI PURNAMANINGSIH Rohim Firdaus Syarifah Iis Aisyah