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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmiah Aplikasi Isotop Radiasi Indonesian Journal of Agricultural Science Jurnal Hortikultura Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri Buletin Iptek Tanaman Pangan AGRIVITA, Journal of Agricultural Science BERITA BIOLOGI Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Penelitian Pertanian Tanaman Pangan Biosaintifika: Journal of Biology & Biology Education Jurnal Penelitian Tanaman Industri (Littri)

RAGAPADMI PURNAMANINGSIH

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Author-ID : 349167

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology

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REGENERATION OF Pimpinella pruatjan THROUGH SOMATIC EMBRYOGENESIS Roostika, I.; Purnamaningsih, R.; Darwati, I.; Mariska, I.
Indonesian Journal of Agricultural Science Vol 8, No 2 (2007): October 2007
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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Pruatjan (Pimpinella pruatjan Molk.) is an Indonesian endangeredplant which has various medicinal properties such asaphrodisiac, diuretic, and tonic. The plant is commonly harvestedfrom its natural habitat, therefore it becomes endangered. Regenerationof pruatjan through organogenesis has been studied,but its shoot multiplication was very low (5 shoots per explant).The study aimed to investigate the best regeneration techniqueof pruatjan through somatic embryogenesis. This research wasconducted at the tissue culture laboratory, Indonesian Centerfor Agricultural Biotechnology and Genetic Resources Researchand Development in 2004-2005. Callus formation of pruatjanwas induced from the petioles and leaves in Driver andKuniyakiâs (DKW) based medium containing 2,4-D combinedwith picloram at the level of 0.5, 1.0, 1.5, and 1.5 ppm. Embryogeniccalli were then transferred into embryo developmentmedium in two ways. First, they were directly transferred intomedia containing IBA/NAA at the level of 0.5, 1.0, and 1.5 ppm.Second, they were indirectly transferred into media containing2.0 ppm 2,4-D and 0.3% casein hydrolysate prior to the IBA/NAA media. Parameters evaluated were fresh weight, dryweight, time initiation of embryogenic callus formation, andtotal number of embryos. The result showed that calli ofpruatjan were successfully induced from the petioles and leaves.The best calli were induced from the leaves in the DKWmedium containing 2.0 ppm 2,4-D and 0.5 ppm picloram.Embryo development of the calli was best if they were firstgrown in the media containing 2.0 ppm 2,4-D and 0.3% caseinhydrolysate then transferred to the IBA/NAA media. The totalnumber of somatic embryos was counted up to 103 on themedium containing 1.5 ppm IBA. This study indicated thatpruatjan somatic embryogenesis regeneration required threedifferent media, i.e. for callus induction, development andmaturation, and for germination.

Pembentukan Benih Sintetik Tanaman Nenas Roostika, Ika; Purnamaningsih, R; Supriati, Y; Mariska, Ika; Khumaida, N; Wattimena, GA
Jurnal Hortikultura Vol 22, No 4 (2012): Desember
Publisher : Indonesian Center for Horticultural Research and Development

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Nenas merupakan tanaman buah tropis dan subtropis yang komersial. Kultivar Smooth Cayenne memiliki tipe dan jumlah propagul yang terbatas, sehingga diperlukan dukungan teknologi lainnya untuk produksi benih secara masal. Teknologi benih sintetik dapat diterapkan untuk produksi benih secara masal dan konservasi. Tujuan Â penelitian ialah untuk mengetahui pengaruh kombinasi auksin dan sitokinin terhadap morfogenesis eksplan nenas yang terenkapsulasi, mengetahui pengaruh interaksi antara suhu penyimpanan dengan konsentrasi paklobutrazol atau manitol terhadap pertumbuhan eksplan nenas yang terenkapsulasi dan masa simpan. Penelitian dilaksanakan dari Bulan April sampai dengan Desember 2011 di Laboratorium Kultur Jaringan, Kelompok Peneliti Biologi Sel dan Jaringan, Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Bogor. Percobaan disusun secara faktorial dalam rancangan acak lengkap terdiri atas enkapsulasi eksplan, pertumbuhan minimal menggunakan paklobutrazol, atau manitol yang dikombinasikan dengan suhu penyimpanan. Enkapsulasi dilakukan terhadap batang semu dan basal daun menggunakan Na-alginat 3% yang berisi media MS dengan penambahan BA (0, 1, 2, dan 3 mg/l) yang dikombinasikan dengan NAA (0, 1, 2, dan 3 mg/l). Untuk memacu proses diferensiasi, basal daun diberi praperlakuan menggunakan media MS yang mengandung BA 0,5 mg/l dan NAA 0,5 mg/l sebelum dienkapsulasi dengan perlakuan BA dan NAA pada konsentrasi 0; 0,5; dan 1 mg/l. Â Pertumbuhan minimal dilakukan menggunakan paklobutrazol (0, 1, 2, dan 3 mg/l) atau manitol (0, 1, 2, 3, 4, dan 5%) pada suhu penyimpanan 15 dan 25 0C. Hasil penelitian menunjukkan bahwa basal daun nenas yang terenkapsulasi mampu berdiferensiasi setelah praperlakuan. Tidak terdapat interaksi yang nyata antara konsentrasi paklobutrazol dengan suhu penyimpanan terhadap daya hidup dan daya tembus kapsul tunas nenas. Biakan tersebut hanya dapat disimpan selama 1 bulan. Interaksi yang nyata juga tidak dijumpai antara konsentrasi manitol dengan suhu penyimpanan terhadap daya hidup dan daya tembus kapsul embrio somatik nenas. Manitol 4% mampu memperpanjang masa simpan hingga 4 bulan. Manitol dapat menggantikan aplikasi suhu rendah dalam penyimpanan kultur nenas yang terenkapsulasi.Â Pineapple is a commercial tropical and subtropical fruit crop. Smooth Cayenne cultivar has limited type and number of propagules so that it should be supported by the other technology to produce plenty seedlings. Artificial seed can be applied for seed production and conservation. The objectives of the study were to know the effect of combination treatments between auxin and cytokinin to the morphogenesis of encapsulated pineapple cultures, to know the effect of paclobutrazol, mannitol, and temperature of storage to the growth of encapsulated pineapple cultures. The experiment was conducted from April to December 2011 at Tissue Culture Laboratory, Researchers Group of Cell and Tissue of Biology, Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development, Bogor. Factorial of a completey randomized design was used. The study consisted of encapsulation, minimal growth by using paclobutrazol or mannitol combined with storage temperature. Encapsulation was conducted by using 3% Na-alginat containing of MS medium with addition of BA (0, 1, 2, and 3 mg/l) combined with NAA (0, 1, 2, and 3 mg/l). To promote differentiation, leaf bases were pre-cultured on MS media containing BA and NAA at concentration of 0.5 mg/l respectively prior to encapsulated by BA and NAA (0; 0.5; and 1 mg/l). Minimal growth was conducted by using paclobutrazol (0, 1, 2, and 3 mg/l), or mannitol (0, 1, 2, 3, 4, and 5%), and combined with storage temperature (15 and 25 0C). The results showed that encapsulated leaf bases of pineapple could differentiate after pre-treatment. There was no interaction between paclobutrazol and temperature to the survival rate and emergence rate of the encapsulated cultures. The encapsulated shoots could be stored for 1 months. There was also no interaction between mannitol and temperature to the survival rate and emergence rate of the encapsulated cultures. By using somatic embryos and 4% mannitol, the storage period could be prolonged for 4 months. Mannitol could substitute the use of low temperature in the conservation of encapsulated pineapple cultures.

Regenerasi Tanaman Sedap Malam Melalui Organogenesis dan Embriogenesis Somatik Rostika, Ika; Mariska, Ika; Purnamaningsih, R
Jurnal Hortikultura Vol 15, No 4 (2005): Desember 2005
Publisher : Indonesian Center for Horticultural Research and Development

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Secara konvensional perbanyakan tanaman sedap malam dilakukan melalui umbi. Semakin kecil ukuran umbi semakin lama tanaman berbunga. Penerapan teknik kultur in vitro diharapkan dapat membantu perbanyakan tanaman secara masal. Hingga saat ini, teknik kultur in vitro tanaman sedap malam belum pernah dilaporkan di Indonesia. Penelitian ini bertujuan memperoleh formulasi media yang efektif menginduksi organogenesis dan embriogenesis kultur in vitro tanaman sedap malam serta memacu regenerasinya. Percobaan dibagi menjadi 4 tahap, yaitu (1) induksi tunas, (2) multiplikasi tunas, (3) induksi kalus embriogenik, dan (4) regenerasi kalus embriogenik. Media induksi tunas yang diuji adalah MS+BA 0 ppm, MS+BA 3 ppm, MS+BA 5 ppm, dan MS+BA 7 ppm. Pemacuan multiplikasi tunas lanjut dilakukan pada media subkultur MS+BA 7 ppm+glutamin 100 ppm, MS+BA 7 ppm, DKW+TDZ 7 ppm, dan DKW+TDZ 7 ppm+glutamin 100 ppm. Untuk induksi kalus embriogenik, media induksi kalus yang diujikan adalah MS+2,4-D 2,5 ppm, MS +2,4-D 5 ppm, dan MS+2,4-D 10 ppm. Untuk meregenerasikan kalus embriogenik, media yang diujikan MS+BA 2 ppm+TDZ 0,2 ppm, MS+BA 3 ppm+TDZ 0,4 ppm, MS+zeatin 1ppm+kinetin 1ppm, dan MS+zeatin 0,5 ppm+kinetin 2 ppm. Hasil percobaan menunjukkan bahwa pembentukan tunas terbanyak diperoleh dari media BA 3 ppm (80%) namun inisiasi tunas tercepat dihasilkan pada media BA 0 ppm. Formula media MS+BA 7 ppm+glutamin 100 ppm menghasilkan jumlah tunas dan akar terbanyak. Penggunaan MS+2,4-D 5 ppm dapat menginduksi kalus embriogenik dengan persentase pembentukan nodul sebesar 18,75% dan jumlah nodul yang terbentuk sebanyak 3,6 dengan visual kalus yang paling baik. Setelah disubkultur, calon tunas terbanyak (17) dihasilkan dari perlakuan MS+BA 2 ppm+TDZ 0,4 ppm. Kalus embriogenik pada media MS+zeatin 0,5 ppm+kinetin 2 ppm dapat berkembang membentuk benih somatik.Regeneration of tuberose through organogenesis and embryogenesis. Tuberose is normally propagated by the tuber. The smaller size of tuber the longer time plant to flower. The application of in vitro culture technique might be used for mass propagation. Up to know, the research of in vitro culture of tuberose in Indonesia has not been reported. The objective of the study was to find out media formulation for organogenesis and embryogenesis. The experiments consisted of 4 steps of (1) shoot induction, (2) shoot multiplication, (3) induction of embryogenic callus, and (4) regeneration of embryogenic callus. The treatments for shoot induction were MS+BA 0 ppm, MS+BA 3 ppm, MS+BA 5 ppm, and MS+BA 7 ppm. The shoots were multiplied on media MS+BA 7ppm+glutamine 100ppm, MS+BA 7 ppm, DKW+TDZ 7 ppm, and DKW+TDZ 7 ppm+glutamin 100 ppm. For induction of embryogenic callus, the treatments were MS+2.4-D 2.5 ppm, MS+2,4-D 5 ppm, and MS+2.4-D 10 ppm. For regeneration of embryogenic callus, the treatments were MS+BA 2 ppm+TDZ 0.2 ppm, MS+BA 3 ppm +TDZ 0.4 ppm, MS+zeatin 1ppm+kinetin 1ppm, and MS+zeatin 0.5 ppm+kinetin 2 ppm. The results showed that the highest shoot formation was obtained from media MS+BA 3 ppm but the earliest shoot initiation was obtained from media MS+BA 0 ppm. The media formulation of MS+BA 7 ppm+glutamine 100 ppm gave the highest number of shoot and root. The application of media MS+2.4-D 5 ppm could induce embryogenic callus with high percentage of nodul formation (18.75%) and high number of nodul (3.6) with the best visual calli. After subculturing, the highest number of nodul (17) was obtained from media MS+BA 2 ppm+TDZ 0.4 ppm. The embryogenic callus from media MS+zeatin 0.5 ppm+kinetin 2 ppm could develop to form somatic seed.

Adaptability of Mutant Genotypes of Artemisia (Artemisia annua L.) as Result Of Gamma Irradiation in Three Locations with Different Altitude Syukur, Muhamad; Lestari, Endang Gati; Purnamaningsih, Ragapadmi; Yunita, Rosa; Aisyah, Syarifah Iis; Firdaus, Rohim
AGRIVITA, Journal of Agricultural Science Vol 33, No 3 (2011)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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The objective of this study was to identify the adaptability of twelve artemisia mutant genotypes, which were planted in three locations with different altitude, as a result of gamma irradiation. Randomized Complete Block Design (RCBD) was applied in this research with three replications as blocks. The genotypes 1B, 1C, 1D, 2, 3, 4, 5A, 6B, 7A, 8, 14, 15 and two control genotypes as parent genotype from seed and from in vitro were used. The genotypes were planted in three different locations such as Mount Putri, Cianjur (1450 m above sea level), Pacet, Cianjur (950 m above sea level) and Cicurug, Sukabumi (540 m above sea level). Based on the method of postdictive and predictive success, the model used was AMMI2 which was able to explain up to 100% of interaction-influenced variation. The genotypes which were found stabile and adaptive in these three locations were 1B, 1C, 1D, 6B and 15. Genotypes 3 and 7A were adaptive specifically in Pacet area, 5A was adaptive for Gunung Putri while genotype 4 was for Cicurug only.Keywords: AMMI, Artemisia annua, mutant genotype, adaptability

INDUKSI MUTASI DAN SELEKSI IN VITRO TANAMAN GANDUM (Triticum aestivum L.) Sari, Laela; Purwito, Agus; Soepandi, Didy; Purnamaningsih, Ragapadmi; Sudarmonowati, Enny
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 3, No 2 (2016): December 2016
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

INDUCTION MUTATION AND SELECTION OF IN VITRO PLANT OF WHEAT (Triticum aestivumÂ L.)The goal of this research was to produce wheat crop which is tolerant to lowland condition.Six varieties were used, Dewata, Selayar, Alibey, Oasis, Rabe and HP1744. This research consisted of 4 stages: production of the best callus on MS medium containing 3 g/L 2.4-D, induced mutation of embryogenic callus using EMS, in vitro selection of callus at temperature of 27â35Â°C, and callus regeneration. The best result for callus production was 76% for Dewata and 70% for Selayar varieties. Higher concentration of EMS and longer soaking time decreased the percentage of callus growth. LC50 for Dewata was 0.3% EMS at 30 minutes and that for Selayar was 0.1% EMS at 60 minutes. The higher the temperature was, the lower was the adaptation tolerant of the plants, and callus growth was inhibited. At the highest temperature (35Â°C) the callus did not grow at all.Keywords: Induced mutation, Triticum aestivum, EMS, in vitro selection, callusABSTRAKTujuan penelitian ini adalah untuk merakit tanaman gandum yang toleran pada dataran rendah. Varietas yang digunakan ada 6 yaitu Dewata, Selayar, Alibey, Oasis, Rabe dan HP-1744. Penelitian terdiri atas empat tahap yaitu induksi pembentukan kalus terbaik menggunakan media MS + 3 g/L 2,4-D (dipilih dua varietas yang terbaik), induksi mutasi kalus embriogenik menggunakan EMS, seleksi kalus in vitro pada suhu 27â35Â°C, dan regenerasi. Hasil induksi kalus terbaik terdapat pada varietas Dewata sebesar 76% dan Selayar sebesar 70%. Semakin tinggi konsentrasi EMS dan semakin lama waktu perendaman yang digunakan maka semakin menurun persentase pertumbuhan kalus. LC50 varietas Dewata adalah EMS 0,3% waktu 30 menit sedangkan LC50 varietas Selayar adalah EMS 0,1% waktu 60 menit.Semakin tinggi suhunya maka semakin berkurang toleran adaptasi tanaman tersebut, dan pertumbuhan kalus semakin sedikit. Bahkan pada suhu tertinggi yaitu suhu 35Â°C tidak ada pertumbuhan kalus sama sekali.Kata Kunci: Induksi mutasi, Triticum aestivum, EMS, seleksi in vitro, kalus

INDUKSI KERAGAMAN SOMAKLONAL DENGAN IRADIASI SINAR GAMMA DAN SELEKSI IN VITRO KALUS PISANG RAJABULU MENGGUNAKAN ASAM FUSARAT, SERTA REGENERASI DAN AKLIMATISASI PLANTLET Lestari, Endang G; Purnamaningsih, R; Mariska, I; Hutami, Sri
BERITA BIOLOGI Vol 9, No 4 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Pisang raja bulu is one of the most important bananas in Indonesia. However, this plant has low toleranee to wilt disease, caused by Fusarium oxysporum f. eubense. Its mass cultivation is inhibited by the absence of variety tolerant to the disease.A wide range of genetic variability will be needed if selection for novel characters is to be conducted, especially when there is no source of resistance gene available for breeding materials. This research consisted of callus induction from primary explant, induction of somaclonal variation using gamma iradiation, and in vitro selection using fusaric acid, followed by regeneration and acclimatization of selected plantlets. The media applied for callus induction was MS (Murashige and Skoog. 1962) + 2,4-D I and 3 mg/l + NAA 0 and 0.1 mg/l and 2,4-D 5 mg/l + BA 0.5 mg/l + Casein hidrolysate (CH) 500 mg/l. The applied gamma irradiation dosage were 0, 5.0. 7.5. 10 and 15 Gy. The irradiated calli was subsequently subcultures on selection media i.e.. MS containing fusaric acid at 30 and 45 mg/l. The living calli was then regenerated on media containing BA, TDZ. with or without proline and arginine. In addition. MS+ kinetin 5 mg/l + 1AA 0,2 mg/l was applied for shoot development. The result showed that the most suitable callus induction media for pisang raja bulu was MS +2.4-D 5 mg/l +BA 0.5 mg/l +CH 500 mg/l. The gamma irradiation of 10 Gy produced somaclone lines which were able to proliferate bud nodules on selection media containing fusaric acid at 30 and 45 mg/l. The media used for shoot development was MS + kinetin 5 mg/l + IAA 0,2 mg/l. Planllet obtained from the in vitro were then successfully acclimatized in the green house.

PENGARUH BAP DAN NAA TERHADAP INDUKSI KALUS DAN KANDUNGAN ARTEMISININ DARI Artemisia annua L. Purnamaningsih, Ragapadmi; Ashrina, Misky
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Malaria is a global health problem that threatens 300-500 million people and kills more than one million people annually.Artemisinin, a sesquiterpen secondary plant metabolite extracted from Artemisia annua L., is a promising and potent antimalarial drug which has a remarkable activity against chloroquine resistant to Plasmodium falciparum. To counter the present low content(0.01-0.5%) of artemisinin in A. annua L.is a limitation to commercial production of the drug and uneconomical chemical synthesis. A research was conducted to induce callus production by using Murashige-Skoog (MS) medium added with NAA (0, 0.5 and 1 mg/1) and BAP (0, 0.5 dan 1 mg/1) and also to produce artemisinin from the calli. Complete Randomized Design was used in the research. Callus cultures were induced from leaf explants of A. annua. The research reports succesful approach for production of artemisinin by callus cultures of A. Annua. Medium formulation of MS basal media added with plant growth regulators BAP 0.5 mg/1 and NAA 0.5 mg/1 give the best result for callus induction than others, with callus fresh weight 844,4 mg, artemisinin content 0.73%, dry weight 216.6 mg and total weight of artemisinin 1.58 mg.

REGENERASI TANAMAN PEPAYA HASIL TRANSFORMASI DENGAN GEN ACC OKSIDASE ANTISENSE [Regeneration of Transforman Papaya Plant with ACC Oxidase Antisense Gene] Purnamaningsih, Ragapadmi; Mariska, Ika; Hutami, Sri
BERITA BIOLOGI Vol 7, No 5 (2005)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Papaya is climacteric fruit. As the other climacteric fruit, papaya has hight speed ripening, so papaya fruit can not stored in long period. Genetic enginering is one alternative technology to solve the problem by introducing antisense oxidase ACC gen to the papaya plant genome to get delay ripening characteristic. Success of genetic enginering technology depend on plant regeneration system.There were two ways of plant regeneration: organogenesis and somatic embryogenesis. The aim of this experiment was to induce root formation of papaya planlet which trasformated by ACC oxidase antisense gene.The former experiment showed that explant which transformated by ACC oxidase antisense gene can regenerated to be shoot/planlet with P6 medium.But when the shoot transferred to root induction medium the root was difficult to formed, callus was formed at the base of shoot, the leaves turn to yellow and fall down.Many media formulations were tried in this experiment with different basic medium for root induction and development.MS (1, Vi) DKW (1, A) and WPM (1, Vi) were used as basic media combined with sucrose (2 % and 3 %) and plant growth regulators (kinetin, IAA, and paclobutrazol) adding with some organic compound. Result of the experiment showed that MS Vi + paclobutrazol 0.5 mg/1 induced root formation 80 %, inhibited callus formation and decreased yellowing and falling of the leaves.

PENGGUNAAN PACLOBUTRAZOLDAN ABA DAL AM PERBANYAKAN X NENAS SIMADU MELALUI KULTUR IN VITRO Purnamaningsih, Ragapadmi; Mariska, Ika; Supriati, Yati
BERITA BIOLOGI Vol 9, No 6 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Pineapple (Ananas comosus L. Merr.), represents an important crop in Subang. Somaclonal variation is one of the problem to develop pineapple, especially Simadu variety. Probability to conduct Simadu progeny from the mother plant is very low (5%).Its caused by chimeric of the somatic cells that form meristem.In vitro culture is the alternative method to solve the problem by using the meristem cells from Simadu fruit as explant. Unfortunately, genetic diversity has been observed in many spesies during tissue culture.This phenomenon is usually termed somaclonal variation. Many studies on pineapple demonstrsted that some in vitro propagated materials differ from the source materials from which they are derived.To minimize genetic variability, the use of growth inhibitor such as paclobutazol and absisic acid hopefully would gave the important role in genetic stability. The aim of the research is to multiply Simadu pineapple by using tissue culture technic. In vitro shoot induce from crown of the Simadu fruit until get the sterile shoots. Combination of kinetin (0-5 ppm) with paclobutrazol ( 0-0.1 ppm) or ABA (0-1 ppm) was used in the multiplication stage. Result showed that there are no interaction between kinetin and paclobutrazol or ABA, but there is influence of the single factor. Kinetin increase leave number but decrease plant height and root number. Paclobutrazol increase shoot and leave number, but decrease plant height and root number. There is no influence of ABA to plant height, shoot and root number but decreased leaves number.

Agronomic Characterization of Wheat Mutants (Triticum aestivum) of M3 Generation Planted in Sukabumi Sari, Laela; Purwito, Agus; Sopandie, Didy; Purnamaningsih, Ragapadmi; Sudarmonowati, Enny
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6612

The purpose of this study was to identify the selection criteria to obtain a superior mutant derived from the wheat plants of such varieties as Dewata, Selayar and Alibey, adaptive in medium land. The analysis of agronomic growth characters showed a significantly effect on a growth percentage of the initial growth (8 mutants), flowering time (1 mutant), panicle stem length (15 mutants), number of panicles (7 mutants), the number of grains per panicle (8 mutants), grain weight observed (8 mutants), grain weight per genotype (6 mutants), leaf area (2 mutants) and leaf greenness (5 mutants). The effects on the characters of ripe time, harvest, panicle length and plant height were not significant. The mutants of Dewata, Selayar and Alibey could be selected based on the characters of panicle stem length, number of grains per panicle and grain weight per observation because these characters generated more mutants than the other characters. The correlation analysis between the characters of growth and yield components of wheat mutants showed that the number of grains per panicle was positively correlated with the grain weight observed, while the length of panicle stem was positively correlated with grain weight per genotype, number of panicles and leaf area. Hopefully some mutants produced could adapt to the tropical medium land, thus adding to the diversity of wheat germplasm in Indonesia, thereby reducing the import of wheat to Indonesia.How to CiteSari, L., Purwito, A., Sopandie, D., Purnamaningsih, R. & Sudarmonowati, E. (2016). Agronomic Characterization of Wheat Mutants (Triticum aestivum) of M3 Generation Planted in Sukabumi. Biosaintifika: Journal of Biology & Biology Education, 8(3), 353-361.

Co-Authors A G Wattimena Agus Purwito Ashrina, Misky Azrai, Muh. DANNY LAURENT Darliah Darliah Deden Sukmadjaja Diantina, Surya Didy Soepandi Didy Soepandi, Didy Didy Sopandie E.G. Lestari E.G. Lestari E.G. Lestari Endang G Lestari Endang G Lestari Endang Gati Lestari Endang Gati Lestari ENDANG GATI LESTARI ENDANG GATI LESTARI ENDANG GATI LESTARI Enny Sudarmonowati Enny Sudarmonowati GA Wattimena Hutami, Sri I Mariska I Roostika I. Darwati I. Darwati I. DARWATI I. Mariska I. Roostika I. Roostika I. ROOSTIKA Ika Mariska Ika Mariska Ika Mariska Ika Mariska Ika Mariska Ika Roostika Ika Roostika Ika Rostika Ika Rostika IRENG DARWATI Ireng Darwati Laela Sari laela Sari, laela Lestari, Endang Gati LESTARI, ENDANG GATI Lizawati . Mariska, I Mariska, Ika MARISKA, IKA Muhamad Syukur Muhammad Syukur N Khumaida N Khumaida NESTI FRONIKA SIANIPAR Nesti Fronika Sianipar NESTI FRONIKA SIANIPAR Noviati, Arief V. Nur, Amin Nurhayani, Siti Pardal, S.J. Pardal, Saptowo Jumali Rita Megia RITA MEGIA ROHIM FIRDAUS ROHIM FIRDAUS Rohim Firdaus Roostika, Ika Rosa Yunita Rosa Yunita Rosa Yunita Rosaria Rosiana Rosaria Rosiana, Rosaria ROSSA YUNITA Rossa Yunita S.J. Pardal Sari, Laela Siti Nurhayani Slamet . Slamet Slamet Slamet, nFN Soeranto, Soeranto Sri Hutami Sri Hutami Sri Hutami Subagio, Herman Sudarmonowati, Enny Sustiprajitno, Sustiprajitno Syarifah Iis Aisyah Tri P. Priyatno Trias Novita Trikoesoemaningtyas UTAMI, SRI Wahyu Handayati Wahyu Handayati Y Supriati Y Supriati Yati Supriati, Yati Yunita, Rossa

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