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All Journal Journal of the Indonesian Tropical Animal Agriculture Agrotech Journal
S. Suhardi
Information Technology Research Group, School of Electrical Engineering and Informatics, Institut Teknologi Bandung
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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mtDNA D-loop sequence analysis of Kalang, Krayan, and Thale Noi buffaloes (Bubalus bubalis) in Indonesia and Thailand reveal genetic diversity Suhardi, S.; Summpunn, P.; Wuthisuthimethavee, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 46, No 2 (2021): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.46.2.93-105

Abstract

Kalang (KBuf), Krayan (KrBuf), and Thale Noi buffaloes (TBuf) are swamp buffalo genetic resources in Indonesia and Thailand. The maternally inherited mitochondrial DNA (mtDNA), particularly D-loop region is an important material for phylogenetic inference and analyzing genetic diversity. Therefore, the objectives of the present study were to evaluate genetic diversity and to reconstruct the phylogenetic tree within buffalo breeds in Kalimantan, Indonesia, and Phatthalung, Thailand using mtDNA D-loop sequences. A total of one hundred forty buffaloes (70 males and 70 females) were observed including 40 buffaloes from North (NK), 40 from East (EK), and 40 from South Kalimantan (SK) provinces Indonesia and 20 from Phatthalung (PT) province, Thailand. DNA samples were isolated from buffalo tail hairs. DNA sequences were manually assembled using BioEdit program with consideration of gaps and ambiguous sequences. The phylogenetic tree of buffalo was generated by PHYLIP software. The observed variables included haplotype diversity, genetic distance, and genetic tree. The 956 bp of amplified mtDNA D-loop fragment presented a total of 24 haplotypes with several mutations that included transitions (293), transversions (60), deletions (15), and insertions (20). The neighbor-joining tree using the Kimura 2 parameter model demonstrated two local buffalo clusters among buffalo from Kalimantan and Thailand with four buffalo relationship patterns observed from buffaloes in Kalimantan Island (KBuf and KrBuf), Indonesia. The Results of the present study demonstrated that the buffaloes sequence analysis revealed relatively high diversity and is a good basis to perform selection and modern buffalo breeding development.
The Critical Period of Hybrid Maize Varieties Against Weeds Sepe, Muslimin; Bulawan, Juniaty Arruan; Suhardi, S.
Agrotech Journal Vol 8, No 2 (2023): Agrotech Journal
Publisher : Universitas Sembilanbelas November Kolaka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31327/atj.v8i2.2112

Abstract

The corn crop cannot be separated from the presence of weeds as crop disturbers. The presence of weeds often causes a decrease in yield and seed quality in corn crops. The yield reduction depends on the type of weed, density, duration of competition, and allelopathic compounds released by weeds. This study aimed to determine the age of the critical period of competition of hybrid varieties of corn plants against weeds, as well as corn grain production due to competition with weeds. The research was conducted in a group randomized design consisting of seven weed control treatments based on corn planting age and repeated three times. Observation parameters consisted of plant height, number of leaves, cob length, cob weight, and cob circumference. The observation results were analyzed followed by Least Significant Different (LSD) at 0.05 percent level. The test results showed that the critical period of competition of corn plants against weeds occurred at the age of 20-45 days after planting. The highest average corn cob weight occurred in the weed control treatment in the third week after planting which was 126.67 gr. There was a compatibility between corn plants and weeds in the form of competition for nutrients and growing space, which affects production
Co-Authors Bulawan, Juniaty Arruan Sepe, Muslimin Summpunn, P. Wuthisuthimethavee, S.