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Journal : Prosiding Seminar Nasional Pakar

UJI SENYAWA ANTIMIKROBA DARI ASAM LEMAK DAN FATTY ACID METHYL ESTER (FAME) MIKROALGA Nannochloropsis oculata Zulkarnain Chaidir; Sari Rahmi; Marniati Salim
Prosiding Seminar Nasional Pakar Prosiding Seminar Nasional Pakar 2019 Buku I
Publisher : Lembaga Penelitian Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/pakar.v0i0.4144

Abstract

Microalgae contains many bioactive molecules including lipid that can be useful for development of antimicrobial compounds. The aim of this research is to test antimicrobial compounds from fatty acid and fatty acid methyl ester (FAME)from microalgae Nannochloropsis oculata. Lipid from microalgae was extracted using hexane and transesterfied using methanol dan sulfuric acid p.a as catalist. Antimicrobial activities were tested using disc diffusion methode against Staphylococcus aureus, Escherichia coli, and yeast Candida albicans, at varying concentrations are 100, 200, and 300 mg/L. The result shown that fatty acids and FAME from microalgae Nannochloropsis oculata have antibacterial activity and do not have anti-candidal activity against Candida albicans. Antibacterial activity of fatty acids is higher than antibacterial activity of FAME on both bacteria. The result shown that fatty acids and FAME contained in lipid of microalgae Nannochloropsis oculata are palmitic acid, stearic acid, methyl lauric, and methyl stearic.
PENAMBAHAN PUPUK KOMERSIAL PADA MEDIUM BBM UNTUK PERTUMBUHAN SPIRULINA PLANTENSIS DAN UJI AKTIVITAS ANTIOKSIDAN Elida Mardiah; Nur Fadillah Pulungan; Marniati Salim
Prosiding Seminar Nasional Pakar Prosiding Seminar Nasional Pakar 2019 Buku I
Publisher : Lembaga Penelitian Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/pakar.v0i0.4153

Abstract

The productivity and quality of Spirulina platensis is determined by the medium growth. One nutrient that can be used for growth Spirulina platensis microalgae are commercial fertilizers including Urea, ZA and TSP. The nitrogen contained in Urea and ZA fertilizers and phosphate in TSP fertilizer will used as an additional nutrient into the growth medium. This research aims to optimize the Spirulina platensis growth medium and test antioxidant activity. Spirulina platensis extract is obtained by sonication and maceration with methanol solvent. The antioxidant activity test was carried out withcalculate IC.50 by spectrophotometry by method1, 1-diphenyl-2-picrylhydrazyl (DPPH). The results showed that fertilizervariations ontreatments P1, P2, P3, P4, P5 and P6 provide optimum growthattreatment P5 (40% urea, 10% ZA and 40% TSP). Spirulina platensis growthon BBM, P5 and BBM medium combined with P5highest cell density on different days of growth. highest is availableon medium C (50% BBM + P5 50%) on day 11. Antioxidant activity inmedium C69,103m g / l, antioxidant activities are classified strong.