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All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Riset Kimia Jurnal Litbang Industri CHEMPUBLISH JOURNAL Chimica et Natura Acta Fullerene Journal of Chemistry Hydrogen: Jurnal Kependidikan Kimia Jurnal Litbang Industri Indonesian Journal of Chemical Studies (Indones. J. Chem. Stud.) Journal of The Indonesian Society of Integrated Chemistry Jurnal Kimia Unand Jurnal Sains dan Kesehatan
Syafrizayanti, Syafrizayanti
Departemen Kimia, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Andalas Kampus Limau Manis, Padang, Sumatera Barat, 25163, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology

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Pengaruh Pemberian Variasi pH terhadap Produksi Trigliserida Total dan Komposisi Asam Lemak dari Chlorella Vulgaris Air Tawar Wulandari, Rahmadani; Dharma, Abdi; Syafrizayanti, Syafrizayanti
Jurnal Riset Kimia Vol 10, No 2 (2019): September
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v10i2.316

Abstract

Chlorella vulgaris is a microalgae that has high lipid content and potential as raw material for biofuel production. This study aims are to determine the effect of pH on growth, lipid production and fatty acid composition of C. vulgaris by using Growmore 32-10-10 fertilizer as a culture medium. Microalgae were cultured in medium Growmore 32-10-10 for 10 days. Afterward, pH of medium was varied into pH 5, 7, 8.2 and 9 and continued cultivate for 3 days. C. vulgaris cultured at pH 8.2 which is a control pH reached optimum growths. The GC-MS analysis for lipid productivity of C. vulgaris was 0.5020 g/L/day and 0.2902 g/L/day for microalgae grew at pH 8.2 and 9, respectively. Cultures at pH 8.2 and 9 produce methyl hexadecanoate, methyl 9-octadecanoate, methyl octadecanoate, methyl 9,12-octadecadienoate, methyl 9,11-octadecadienoate. Additional fatty acid methyl nonadecanoate was also found in C. vulgaris grew at pH 9. The low and high pH stress of C. vulgaris culture medium did not affect culture growth but altered lipid production and fatty acid composition.
Pemurnian Fikosianin dari Spirulina platensis dengan Metode Liquid Biphasic Flotation (LBF) dan Penentuan Aktivitas Antioksidannya Elise, Cori Yolanda; Syafrizayanti, Syafrizayanti; Salim, Marniati
Jurnal Riset Kimia Vol 12, No 2 (2021): September
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v12i2.412

Abstract

Phycocyanin from Spirulina platensis (S. Platensis) is a pigment-complex protein belong to the light-harvesting phycobiliprotein family. The pigments have high economic value as a natural blue dye as well as the source of antioxidants and anticancer. Production of pure natural phycocyanin remains in high demand. Therefore, this study aimed to obtain phycocyanin with high purity values using modified liquid biphasic flotation (LBF) system and tested for DPPH (1,1-diphenyl-2-pycrilhydrazil) radical scavenging activity. This study produced high purity phycocyanin with purification fold 3.041 ± 0.04 and recovery yields approximately about 70.881%. Purified phycocyanin showed scavenging activity with IC50 of   338.585 mg/mL. Thus, the LBF system yielded high purity phycocyanin pigments.
Isolasi dan Identifikasi Mikroalga Sebagai Sumber Antioksidan dari Perairan Tirtasari Sonsang, Agam, Sumatera Barat Iolantri Handra; Syafrizayanti Syafrizayanti; Zulkarnain Chaidir
Chimica et Natura Acta Vol 7, No 1 (2019)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (501.16 KB) | DOI: 10.24198/cna.v7.n1.20076

Abstract

Mikroalga memiliki potensi sebagai sumber antioksidan, yang dapat mencegah dan menghambat radikal bebas. Peneliti secara personal telah mengisolasi mikroalga dari perairan Tirtasari Sonsang, Sumatra Barat. Pada penelitian ini, isolat mikroalga diidentifikasi secara morfologi dan molekular. Mikroalga ditumbuhkan dalam tiga jenis medium dan diekstrak menggunakan pelarut metanol, etil asetat dan heksana. Potensi antioksidan ekstrak mikroalga tersebut diuji menggunakan metoda DPPH. Berdasarkan hasil identifikasi secara morfologi dan molekular, isolat mikroalga termasuk dalam jenis Chlorella sp.. Aktivitas antioksidan isolat mikroalga yang tumbuh dalam medium BBM, dan diesktrak menggunakan metanol pada konsentrasi 200 mg/L memiliki nilai persen inhibisi terhadap radikal DPPH sebesar 55,8%.
Pengaruh Pemberian Variasi pH terhadap Produksi Trigliserida Total dan Komposisi Asam Lemak dari Chlorella Vulgaris Air Tawar Rahmadani Wulandari; Abdi Dharma; Syafrizayanti Syafrizayanti
Jurnal Riset Kimia Vol. 10 No. 2 (2019): September
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v10i2.316

Abstract

Chlorella vulgaris is a microalgae that has high lipid content and potential as raw material for biofuel production. This study aims are to determine the effect of pH on growth, lipid production and fatty acid composition of C. vulgaris by using Growmore 32-10-10 fertilizer as a culture medium. Microalgae were cultured in medium Growmore 32-10-10 for 10 days. Afterward, pH of medium was varied into pH 5, 7, 8.2 and 9 and continued cultivate for 3 days. C. vulgaris cultured at pH 8.2 which is a control pH reached optimum growths. The GC-MS analysis for lipid productivity of C. vulgaris was 0.5020 g/L/day and 0.2902 g/L/day for microalgae grew at pH 8.2 and 9, respectively. Cultures at pH 8.2 and 9 produce methyl hexadecanoate, methyl 9-octadecanoate, methyl octadecanoate, methyl 9,12-octadecadienoate, methyl 9,11-octadecadienoate. Additional fatty acid methyl nonadecanoate was also found in C. vulgaris grew at pH 9. The low and high pH stress of C. vulgaris culture medium did not affect culture growth but altered lipid production and fatty acid composition.
Pemurnian Fikosianin dari Spirulina platensis dengan Metode Liquid Biphasic Flotation (LBF) dan Penentuan Aktivitas Antioksidannya Cori Yolanda Elise; Syafrizayanti Syafrizayanti; Marniati Salim
Jurnal Riset Kimia Vol. 12 No. 2 (2021): September
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v12i2.412

Abstract

Phycocyanin from Spirulina platensis (S. Platensis) is a pigment-complex protein belong to the light-harvesting phycobiliprotein family. The pigments have high economic value as a natural blue dye as well as the source of antioxidants and anticancer. Production of pure natural phycocyanin remains in high demand. Therefore, this study aimed to obtain phycocyanin with high purity values using modified liquid biphasic flotation (LBF) system and tested for DPPH (1,1-diphenyl-2-pycrilhydrazil) radical scavenging activity. This study produced high purity phycocyanin with purification fold 3.041 ± 0.04 and recovery yields approximately about 70.881%. Purified phycocyanin showed scavenging activity with IC50 of   338.585 mg/mL. Thus, the LBF system yielded high purity phycocyanin pigments.
Isolasi dan identifikasi spesies mikroalga air tawar sebagai antioksidan dan antihiperglikemik Anggela Marta Tasman; Abdi Dharma; Syafrizayanti Syafrizayanti
Jurnal Litbang Industri Vol 10, No 1 (2020)
Publisher : Institution for Industrial Research and Standardization of Industry - Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24960/jli.v10i1.5956.61-71

Abstract

Mikroalga memiliki kinerja yang hampir sama dengan tumbuhan bersel banyak, akan tetapi tidak memiliki akar, daun, dan batang untuk berfotosintesis. Mikroalga diibaratkan sebagai pabrik kecil dalam ukuran sel mikro yang mengubah karbondioksida menjadi material potensial. Penelitian ini bertujuan untuk mengisolasi mikroalga, mengidentifikasi spesiesnya secara morfologi dan molekuler, selanjutnya menentukan kandungan total fenolik, bioaktivitas antioksidan dan antihiperglikemik dari ekstrak mikroalga tersebut. Uji antioksidan dilakukan dengan metode DPPH dan uji antihiperglikemik dengan metode inhibitor enzim α-amilase. Mikroalga yang berhasil diisolasi termasuk dalam jenis Chlorella vulgaris. Ekstrak metanol dari biomassa kering mikroalga memberikan kandungan fenolik total paling tinggi yaitu sebesar 10,8 mg GAE/g jika dibandingkan ekstrak air (1,8 mg GAE/g) dan heksana (1,1 mg GAE/g). Nilai IC50 ekstrak metanol dalam menangkap radikal bebas DPPH adalah 75,9 µg/mL dan mampu menginhibisi 50% aktivitas enzim α-amilase pada konsentrasi 839,9 µg/mL. Berdasarkan hasil uji bioaktivitas ekstrak metanol mikroalga Chlorella vulgaris yang diisolasi dari perairan Sungai Kincir Kamba Tigo memiliki kemampuan yang rendah sebagai antioksidan dan antihiperglikemik.
Isolasi dan identifikasi spesies mikroalga air tawar sebagai antioksidan dan antihiperglikemik Anggela Marta Tasman; Abdi Dharma; Syafrizayanti Syafrizayanti
Jurnal Litbang Industri Vol 10, No 1 (2020)
Publisher : Institution for Industrial Research and Standardization of Industry - Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2279.277 KB) | DOI: 10.24960/jli.v10i1.5956.61-71

Abstract

Mikroalga memiliki kinerja yang hampir sama dengan tumbuhan bersel banyak, akan tetapi tidak memiliki akar, daun, dan batang untuk berfotosintesis. Mikroalga diibaratkan sebagai pabrik kecil dalam ukuran sel mikro yang mengubah karbondioksida menjadi material potensial. Penelitian ini bertujuan untuk mengisolasi mikroalga, mengidentifikasi spesiesnya secara morfologi dan molekuler, selanjutnya menentukan kandungan total fenolik, bioaktivitas antioksidan dan antihiperglikemik dari ekstrak mikroalga tersebut. Uji antioksidan dilakukan dengan metode DPPH dan uji antihiperglikemik dengan metode inhibitor enzim α-amilase. Mikroalga yang berhasil diisolasi termasuk dalam jenis Chlorella vulgaris. Ekstrak metanol dari biomassa kering mikroalga memberikan kandungan fenolik total paling tinggi yaitu sebesar 10,8 mg GAE/g jika dibandingkan ekstrak air (1,8 mg GAE/g) dan heksana (1,1 mg GAE/g). Nilai IC50 ekstrak metanol dalam menangkap radikal bebas DPPH adalah 75,9 µg/mL dan mampu menginhibisi 50% aktivitas enzim α-amilase pada konsentrasi 839,9 µg/mL. Berdasarkan hasil uji bioaktivitas ekstrak metanol mikroalga Chlorella vulgaris yang diisolasi dari perairan Sungai Kincir Kamba Tigo memiliki kemampuan yang rendah sebagai antioksidan dan antihiperglikemik.
Penentuan Kadar Astaxanthin, Uji Antiinflamasi Dan Sitotoksik Terhadap Sel Kanker Payudara Ekstrak Aseton Spirulina Platensis Armaini; Syafrizayanti; Zara Aulia
Jurnal Kimia Unand Vol. 11 No. 1 (2022): May 2022
Publisher : Departemen Kimia Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jku.11.1.25-32.2022

Abstract

Spirulina platensis is a microalgae widely used in the health sector due to its micronutrients, biopigment such as astaxanthin. Astaxanthin is carotenoid belonging to strong antioxidant, which has anti-inflammatory and cytotoxic effects on MCF-7 breast cancer cells. This study aims to determine astaxanthin contents, to investigate the anti-inflammatory activity and cytotoxic potential of breast cancer cells MCF-7 treated with acetone extract from Spirulina platensis. Extraction is done by maceration method. Astaxanthin contents were determined using UV-Vis spectrophotometer set to 479 nm. The anti-inflammatory assays were performed using inhibition of protein denaturation method and cytotoxic assays were performed using the MTT method. From results of study, the extract contained 6,3008 mg/L astaxanthin. The IC50 values for anti-inflammatory and cytotoxic tests ​​were 363,399 mg/L and 66,68 mg/L, respectively. According to the result of the tests, acetone extract from Spirulina platensis showed anti-inflammatory activity and moderate cytotoxic activity against MCF-7 breast cancer cells.
Detection and Absolute Quantification Porcine DNA in Sausages Using Quantitative Polymerase Chain Reaction (qPCR) Method Syafrizayanti; S. Safni; Nurhayati
Jurnal Kimia Unand Vol. 11 No. 2 (2022): November 2022
Publisher : Departemen Kimia Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jku.11.2.5-13.2022

Abstract

Identification of adulteration of processed meat products with unwanted ingredients is a crucial issue. These meat products are prone to forgery and mix with porcine. Meat source authentication is important for Muslim consumers to whom comsumption of products containing pork and its derivatives in an products is prohibited. This present study aims at development of detection and quantification method of porcine DNA (Deoxyribo Nucleic Acid) in processed meat products, sausages. Two novel primer pairs were designed specifically targeting fragment of Displacement Loop (D-Loop) and cytochrome b (cyt b) of porcine mitochondrial DNA and to generate 139 bp and 143 bp amplicons, respectively. Detection and quantification were accomplished by Quantitative Polymerase Chain Reaction (qPCR). Porcine DNA standard curves and cycle threshold were used for quantification. The detection limit of porcine DNA was as little as 0.05 pg. Of all sausages tested (n=13), four of them contained porcine DNA as much 3.1 pg; 0.160 pg; 0.294 pg; and 0.110 pg in 0.001 mg of sausages for J, G, I, and L samples respectively. The specific qPCR assay method can be used for the detection of porcine DNA in minute amounts, which can be used for the halal authentication of food and pharmaceutical products.
Challenges in Molecular and Morphological Identification of Sponge Species in Raja Ampat Aisyah, Siti Zanuba; Zamani, Neviaty Putri; Cahyani, Ni Kadek Dita; Elfahmi, Elfahmi; Syafrizayanti, Syafrizayanti; Andriani, Yosie; Arafat, Dondy; Bashari, Muhammad Hasan; Hanif, Novriyandi; Sani, Lalu M. Iqbal; Ayu, Inna Puspa; Akbar, Nebuchadnezzar; Subhan, Beginer
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 30, No 1 (2025): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.30.1.115-125

Abstract

Sponges (Phylum Porifera) are a group of highly diverse, sessile, and filter-feeding basal metazoans, except spongillids. The majority of sponges are marine species that play an important role in benthic habitat by maintaining the stability of marine ecosystems through nutrient cycling, habitat provision, and bioerosion. However, marine sponges are not easily identifiable because of their lack of species-level distinctive morphological features, which limits efforts to monitor actual species biodiversity. Indonesia is home to approximately 850 identified species of marine sponges, and Raja Ampat archipelago of West Papua province is known for its exceptional marine biodiversity. Despite the species abundance, the exact number of sponges in the region is not well-documented due to the lack of specific studies providing comprehensive data on their diversity. Therefore, this study aimed to conduct a DNA barcoding analysis using the mitochondrial cytochrome oxidase I (COI) gene as a marker, combined with morphological analyses of 22 individuals collected in the waters of Waigeo, Mansuar, and Gam Islands in Raja Ampat. The results showed that 3 samples were identified at the species level (Halichondria sp. and Stylissa carteri) with good query cover and percent identity. This showed the possible presence of undescribed or cryptic species, suggesting a severe lack of reference data for both morphology and molecular analyses of marine sponges in the region. Consequently, the analysis showed the presence of a significant gap in the understanding of sponge biodiversity in Raja Ampat's waters.
Co-Authors Abdi Dharma Abdi Wira Septama Agustian, Dede Rahman Aisyah, Siti Zanuba Aken Putri Nandi Nanti Alfath, Yuma Nur Andre Priandoko Anggela Marta Tasman Arifah, Zil Armaini Armaini Armaini, Armaini Ayu, Inna Puspa Bashari, Muhammad Hasan Beginer Subhan Cori Yolanda Elise Dondy Arafat Efahmi, Efahmi Elfahmi Elfahmi, Elfahmi Elise, Cori Yolanda Geliz Luh Titisari Hillman, Prima Fitria Iolantri Handra Jurnal Riset Kimia, Editor in Chief Kusnanda, Arif Juliari Mai Efdi Mai Efdi Marniati Salim Masyerli, Scify Bilqis Nawafi Mochamad Untung Kurnia Agung Nebuchadnezzar Akbar NEVIATY PUTRI ZAMANI Ni Kadek Dita Cahyani, Ni Kadek Dita Novriyandi Hanif Nurhayati Nurul Haviza Nurul Haviza Rahma, Diny Hazita Rahmadani Wulandari Riga, Riga S. Safni Sani, Lalu M. Iqbal Susanti, Febria Elvy Titisari, Geliz Luh Wulandari, Rahmadani Yosie Andriani Zara Aulia Zulkarnain Chaidir