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Journal : Jurnal Riset Kimia

MEMPELAJARI KONDISI OPTIMUM PEMBUATAN BIOETANOL MELALUI HIDROLISIS ENZIMATIS MENGGUNAKAN Sacharomycess cereviceae DARI PATI SAGU DAN UMBI TALAS Salim, Marniati; Mardiah, Elida; Opthymal, Yosi; Febrizal, Febby
Jurnal Riset Kimia Vol 5, No 1 (2011): September
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v5i1.174

Abstract

Vol 5 No 1ABSTRACT Sago (Metroxylon sp) and umbi talas (Colocasia gigantea Hook F) are natural resources that are found in plantyfull Indonesian. However, these potencies are not yet processed to give an optimum value added product. Sago and taro are rich of starch, that could be fermented to form bioethanol for reneweable energy. The ethanol were produced by fermenting the hydrolized starch of sago and taro with yeast. The starch of sago and taro were hydrolized enzymaticly by α-amylase and glucoamylase. Sago and taro (15 g each) were grinded and hydrolized by α-amylase and then by glucoamylase at the variation of volume of 4, 5, 6, 7, and 8 mL for 1, 2, 3, 4, and 5 hours. The glucose produced was measured by Somogy-Nelson methods. The product of sago hydrolysis were optimum with 6 mL of α-amylase and 6 mL glucoamylase for 2 hours to give 59.11 g/L of reducing sugar. The product of taro with 6 mL α-amylase and 7 mL glucoamylase for 4 hours which gave 64.22 g/L of reducing sugar. The product of ethanol were analyzed by Gas Cromatography (GC). The maximum bioethanol production obtained optimum after 4 days fermentation of hydrolized sago starch and after 5 days fermentation of hydrolized taro starch which were 3.742% and 4.0123%. Key words: Bioethanol, sago starch, taro, enzymatic hydrolysed, α-amylase and glucoamylase
EMISI DAN ABSORPSI GAS METANA PADA SISTEM PENANAMAN PADI DI AREA TANAH SAWAH Salim, Marniati; Faizah, Hiyal; Sudiana, I Made
Jurnal Riset Kimia Vol 4, No 1 (2010): September
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v4i1.74

Abstract

 To know there are emission and methane absorption, research was conduted by measuring directly gas methane at the rice field and absorption with metanotroph bactery using chromatography gas. From the research, we know that there is methane gas emission at the rice field. Watery land condition, emit methane gas (2,309 mg/L) larger than not watery land (0,059 mg/L). At the same time and paddy age is 2 month, it also emit larger methane gas (1,809 mg/L) than 1 month age paddy (1,758 mg/L) and without paddy (0,697 mg/L), whereas for methane absorption at land sample  T0, T1, T2, T3, shows that with fertilizer given can increase the reduction of injected methane gas. Keywords: Absorption, Metanotroph bacteria, Methane gas emission   
PENGARUH HIDROLISIS ENZIMATIS TERHADAP PRODUKSI BIOETANOL DARI UMBI TALAS (Colocasia gigantea Hook F) Salim, Marniati; Mardiah, Elida; Febrizal, Febby
Jurnal Riset Kimia Vol 5, No 2 (2012): March
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v5i2.214

Abstract

Umbi talas (Colocasia gigantea Hook F) are rich of starch (77.9%), that could be fermentated to form bioethanol for reneweable energy. The ethanol were produced by fermenting the hydrolised enzymaticly by α-amylase and glucoamylase at the variation 4, 5, 6, 7, and 8 mL for 1, 2, 3, 4, and 5 hours. The glucose produced was measured by Somogy-Nelson methode. The product of hydrolysed were optimum with 6 mL α-amylase and 7 mL glucoamylase for 4 hours wich gave 64,222 g/L of reducing sugar. The product of ethanol were analyzed by Gas Chromatography (GC). The maximum bioethanol production obtained after 5 days fermentation was 4.0123%.
PRODUKSI BIOETANOL DARI SAMPAH DEDAUNAN SEKITAR KAMPUS UNAND DENGAN METODE SSF (SIMULTANEOUS SACHARIFICATION FERMENTATION) Salim, Marniati; Mardiah, Elida; Atmelwidia, Yollanda
Jurnal Riset Kimia Vol 7, No 2 (2014): March
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v7i2.173

Abstract

Vol 7 No 2ABSTRACTIn this research, cellulose from waste leaves are conversed into ethanol by SSF technologyusing cellulase from Trichoderma viride strain T1 sk. In saccharification process, cellulasebreaks cellulase polymer into glucose. Simultaneously, the formed glucose is conversed intoethanol by invertase produced by Saccharomyces cerevisiaewhich is grew on YPD medium.Waste leaves are pretreatmented using basic solutions : basic NaOH 1 %, NH4OH 8 %, NaOH 1% + NH4OH 4 % and NaOH 1 % + NH4OH 8 % with ratio of solid mather (sample) : liquid(basic solution) 1:10 (w/v) with volume of basic solution 100 mL. Immersion time is variatedfor 24, 48 and 72 hours on 500C. The result of research shows that the use of NaOH 1% +NH4OH 4% gives the highest glucose concentration 933,75 μg/mL with immersion time for 72hours on 500C. After being pretreatmented, sample of 0,4 g waste leaves produce the highestglucose concentration. Measurement by GC/MS shows ethanol concentration 62,41% onfermentation time 96 hours with volume of ethanol 2,45 mL for 0,4 g sample.Keywords : Waste leaves, Pretreatment, Trichoderma viride strain T1 sk, SSF, Bioethanol
Pemurnian Fikosianin dari Spirulina platensis dengan Metode Liquid Biphasic Flotation (LBF) dan Penentuan Aktivitas Antioksidannya Elise, Cori Yolanda; Syafrizayanti, Syafrizayanti; Salim, Marniati
Jurnal Riset Kimia Vol 12, No 2 (2021): September
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v12i2.412

Abstract

Phycocyanin from Spirulina platensis (S. Platensis) is a pigment-complex protein belong to the light-harvesting phycobiliprotein family. The pigments have high economic value as a natural blue dye as well as the source of antioxidants and anticancer. Production of pure natural phycocyanin remains in high demand. Therefore, this study aimed to obtain phycocyanin with high purity values using modified liquid biphasic flotation (LBF) system and tested for DPPH (1,1-diphenyl-2-pycrilhydrazil) radical scavenging activity. This study produced high purity phycocyanin with purification fold 3.041 ± 0.04 and recovery yields approximately about 70.881%. Purified phycocyanin showed scavenging activity with IC50 of   338.585 mg/mL. Thus, the LBF system yielded high purity phycocyanin pigments.
EMISI DAN ABSORPSI GAS METANA PADA SISTEM PENANAMAN PADI DI AREA TANAH SAWAH Marniati Salim; Hiyal Faizah; I Made Sudiana
Jurnal Riset Kimia Vol. 4 No. 1 (2010): September
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v4i1.74

Abstract

 To know there are emission and methane absorption, research was conduted by measuring directly gas methane at the rice field and absorption with metanotroph bactery using chromatography gas. From the research, we know that there is methane gas emission at the rice field. Watery land condition, emit methane gas (2,309 mg/L) larger than not watery land (0,059 mg/L). At the same time and paddy age is 2 month, it also emit larger methane gas (1,809 mg/L) than 1 month age paddy (1,758 mg/L) and without paddy (0,697 mg/L), whereas for methane absorption at land sample  T0, T1, T2, T3, shows that with fertilizer given can increase the reduction of injected methane gas. Keywords: Absorption, Metanotroph bacteria, Methane gas emission   
PRODUKSI BIOETANOL DARI SAMPAH DEDAUNAN SEKITAR KAMPUS UNAND DENGAN METODE SSF (SIMULTANEOUS SACHARIFICATION FERMENTATION) Marniati Salim; Elida Mardiah; Yollanda Atmelwidia
Jurnal Riset Kimia Vol. 7 No. 2 (2014): March
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v7i2.173

Abstract

Vol 7 No 2ABSTRACTIn this research, cellulose from waste leaves are conversed into ethanol by SSF technologyusing cellulase from Trichoderma viride strain T1 sk. In saccharification process, cellulasebreaks cellulase polymer into glucose. Simultaneously, the formed glucose is conversed intoethanol by invertase produced by Saccharomyces cerevisiaewhich is grew on YPD medium.Waste leaves are pretreatmented using basic solutions : basic NaOH 1 %, NH4OH 8 %, NaOH 1% + NH4OH 4 % and NaOH 1 % + NH4OH 8 % with ratio of solid mather (sample) : liquid(basic solution) 1:10 (w/v) with volume of basic solution 100 mL. Immersion time is variatedfor 24, 48 and 72 hours on 500C. The result of research shows that the use of NaOH 1% +NH4OH 4% gives the highest glucose concentration 933,75 μg/mL with immersion time for 72hours on 500C. After being pretreatmented, sample of 0,4 g waste leaves produce the highestglucose concentration. Measurement by GC/MS shows ethanol concentration 62,41% onfermentation time 96 hours with volume of ethanol 2,45 mL for 0,4 g sample.Keywords : Waste leaves, Pretreatment, Trichoderma viride strain T1 sk, SSF, Bioethanol
MEMPELAJARI KONDISI OPTIMUM PEMBUATAN BIOETANOL MELALUI HIDROLISIS ENZIMATIS MENGGUNAKAN Sacharomycess cereviceae DARI PATI SAGU DAN UMBI TALAS Marniati Salim; Elida Mardiah; Yosi Opthymal; Febby Febrizal
Jurnal Riset Kimia Vol. 5 No. 1 (2011): September
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v5i1.174

Abstract

Vol 5 No 1ABSTRACT Sago (Metroxylon sp) and umbi talas (Colocasia gigantea Hook F) are natural resources that are found in plantyfull Indonesian. However, these potencies are not yet processed to give an optimum value added product. Sago and taro are rich of starch, that could be fermented to form bioethanol for reneweable energy. The ethanol were produced by fermenting the hydrolized starch of sago and taro with yeast. The starch of sago and taro were hydrolized enzymaticly by α-amylase and glucoamylase. Sago and taro (15 g each) were grinded and hydrolized by α-amylase and then by glucoamylase at the variation of volume of 4, 5, 6, 7, and 8 mL for 1, 2, 3, 4, and 5 hours. The glucose produced was measured by Somogy-Nelson methods. The product of sago hydrolysis were optimum with 6 mL of α-amylase and 6 mL glucoamylase for 2 hours to give 59.11 g/L of reducing sugar. The product of taro with 6 mL α-amylase and 7 mL glucoamylase for 4 hours which gave 64.22 g/L of reducing sugar. The product of ethanol were analyzed by Gas Cromatography (GC). The maximum bioethanol production obtained optimum after 4 days fermentation of hydrolized sago starch and after 5 days fermentation of hydrolized taro starch which were 3.742% and 4.0123%. Key words: Bioethanol, sago starch, taro, enzymatic hydrolysed, α-amylase and glucoamylase
PENGARUH HIDROLISIS ENZIMATIS TERHADAP PRODUKSI BIOETANOL DARI UMBI TALAS (Colocasia gigantea Hook F) Marniati Salim; Elida Mardiah; Febby Febrizal
Jurnal Riset Kimia Vol. 5 No. 2 (2012): March
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v5i2.214

Abstract

Umbi talas (Colocasia gigantea Hook F) are rich of starch (77.9%), that could be fermentated to form bioethanol for reneweable energy. The ethanol were produced by fermenting the hydrolised enzymaticly by α-amylase and glucoamylase at the variation 4, 5, 6, 7, and 8 mL for 1, 2, 3, 4, and 5 hours. The glucose produced was measured by Somogy-Nelson methode. The product of hydrolysed were optimum with 6 mL α-amylase and 7 mL glucoamylase for 4 hours wich gave 64,222 g/L of reducing sugar. The product of ethanol were analyzed by Gas Chromatography (GC). The maximum bioethanol production obtained after 5 days fermentation was 4.0123%.
Pemurnian Fikosianin dari Spirulina platensis dengan Metode Liquid Biphasic Flotation (LBF) dan Penentuan Aktivitas Antioksidannya Cori Yolanda Elise; Syafrizayanti Syafrizayanti; Marniati Salim
Jurnal Riset Kimia Vol. 12 No. 2 (2021): September
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v12i2.412

Abstract

Phycocyanin from Spirulina platensis (S. Platensis) is a pigment-complex protein belong to the light-harvesting phycobiliprotein family. The pigments have high economic value as a natural blue dye as well as the source of antioxidants and anticancer. Production of pure natural phycocyanin remains in high demand. Therefore, this study aimed to obtain phycocyanin with high purity values using modified liquid biphasic flotation (LBF) system and tested for DPPH (1,1-diphenyl-2-pycrilhydrazil) radical scavenging activity. This study produced high purity phycocyanin with purification fold 3.041 ± 0.04 and recovery yields approximately about 70.881%. Purified phycocyanin showed scavenging activity with IC50 of   338.585 mg/mL. Thus, the LBF system yielded high purity phycocyanin pigments.