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ISOLASI DAN IDENTIFIKASI BAKTERI Vibrio sp DARI IKAN KERAPU SUNU (Plectropomus leopardus) Hidayat, Ar. Syarif
Teknosains Vol 8, No 2 (2014): JULI
Publisher : Fakultas Sains dan Teknologi UIN ALauddin

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Abstract

This research about the isolation and identification Vibrio sp from sunu grouper fish (Plectropomus leopardus). This research aimed to observe the amount of vibrio bacteria species based on colony morphology and identificate morphological colony of vibrio isolated from grouper sunu fish (Plectropomus leopardus). Bacteria isolation was conducted by isolate some external organs (lesion on its body) and internal organ (jejenum) from grouper sunu fish (Plectropomus leopardus). Bacteria was grown on selective medium for vibrio, TCBS (Thiosulfate Citrate Bile Sucrose) agar. The incubation time was 24 hours at 30 C then the morphological colony was observed and biochemestry test was done. Morphology characteristic and biochemistry test result was matched with bacterial characteristic on Bargey’s Manual of Determinative Bacteriology. The result showed there are 3 isolate vibrio bacteria that was coded by V1, V2, V3. V1 morphological characteristis was rounded, edge is entire, convex elevation, green colony colour with blue in the center of colony and smooth tekstur. Based on biochemistry test V1 was indetificated as Vibrio parahaemolyticus. V2 morphological characteristic was rounded, edge is entire, tortous elevation, colony colour yellow and smooth tekstur. based on the biochemistry tes V2 was indentificated as Vibrio harveyi. Morphological characteristic of V3 was Irregular; egde is Undulate, elevation hill, colony colour yellow and own the smooth tekstur. Based on biochemistry test V3 was identificated as Vibrio alginolyticus.Key words: Isolation, Identification, Vibrio, Kerapu Sunu (Plectropomus leopardus).
PENGARUH PEMBERIAN TUAK TERHADAP GAMBARAN HISTOPATOLOGI HATI MENCIT (Mus musculus) ICR JANTAN Sijid, St Aisyah; Muthiadin, Cut; Zulkarnain, Zulkarnain; Hidayat, Ar. Syarif
Jurnal Pendidikan Matematika dan IPA Vol 11, No 2 (2020): Juli 2020
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1146.746 KB) | DOI: 10.26418/jpmipa.v11i2.36623

Abstract

Liver damage or liver disease is caused by many factors, one of which is by consuming drinks that contain alcohol. Tuak is one of the drinks that contain alcohol. This study aims to determine the effect of palm wine administration on the histopathological picture of male ICR mice (Mus musculus) liver. This study used 20 mice consisting of 4 treatments namely P0 = 0 mL / day / head; P1 = 0.1 mL / day / head; P2 = 0.2 mL / day / head and P3 = 0.3 mL / day / head. The parameters observed were body weight of mice, liver weight and liver histopathology of mice. The results showed that administration of palm wine to male ICR mice at different doses gave an influence on the histopathological picture of male ICR mice (Mus musculus).AbstrakKerusakan hati atau penyakit liver disebabkan oleh banyak faktor, salah satunya adalah dengan mengkonsumsi minuman yang mengandung alkohol. Tuak merupakan salah satu minuman yang mengandung alkohol. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian tuak terhadap gambaran histopatologi hati mencit (Mus musculus) ICR jantan. Penelitian ini menggunakan 20 ekor mencit yang terdiri dari 4 perlakuan yaitu P0 = 0 mL/hari/ekor; P1 = 0,1 mL/hari/ekor; P2 = 0,2 mL/hari/ekor dan P3 = 0,3 mL/hari/ekor. Parameter yang diamati adalah berat badan mencit, berat hati dan histopatologi hati mencit. Hasil penelitian menunjukkan bahwa pemberian tuak pada mencit ICR jantan dengan dosis yang berbeda memberikan pengaruh terhadap gambaran histopatologi hati mencit (Mus musculus) ICR jantan.Kata Kunci: Hati, Histopatologi, Mencit, Tuak
ISOLASI DAN IDENTIFIKASI BAKTERI Vibrio sp DARI IKAN KERAPU SUNU (Plectropomus leopardus) Ar. Syarif Hidayat
Teknosains Vol 8 No 2 (2014): JULI
Publisher : Fakultas Sains dan Teknologi Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/teknosains.v8i2.1909

Abstract

This research about the isolation and identification Vibrio sp from sunu grouper fish (Plectropomus leopardus). This research aimed to observe the amount of vibrio bacteria species based on colony morphology and identificate morphological colony of vibrio isolated from grouper sunu fish (Plectropomus leopardus). Bacteria isolation was conducted by isolate some external organs (lesion on its body) and internal organ (jejenum) from grouper sunu fish (Plectropomus leopardus). Bacteria was grown on selective medium for vibrio, TCBS (Thiosulfate Citrate Bile Sucrose) agar. The incubation time was 24 hours at 30 C then the morphological colony was observed and biochemestry test was done. Morphology characteristic and biochemistry test result was matched with bacterial characteristic on Bargey’s Manual of Determinative Bacteriology. The result showed there are 3 isolate vibrio bacteria that was coded by V1, V2, V3. V1 morphological characteristis was rounded, edge is entire, convex elevation, green colony colour with blue in the center of colony and smooth tekstur. Based on biochemistry test V1 was indetificated as Vibrio parahaemolyticus. V2 morphological characteristic was rounded, edge is entire, tortous elevation, colony colour yellow and smooth tekstur. based on the biochemistry tes V2 was indentificated as Vibrio harveyi. Morphological characteristic of V3 was Irregular; egde is Undulate, elevation hill, colony colour yellow and own the smooth tekstur. Based on biochemistry test V3 was identificated as Vibrio alginolyticus.
Struktur komunitas filum Echinodermata di Pulau Barrang Lompo Makassar Ariyanto, Tiara Puspitasari; Saenab, Sitti; Hidayat, Ar. Syarif
Filogeni: Jurnal Mahasiswa Biologi Vol 1 No 1 (2021): Januari-April
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (971.487 KB) | DOI: 10.24252/filogeni.v1i1.20574

Abstract

Peranan Echinodermata di perairan laut adalah sebagai pembersih limbah dan sampah, mempunyai nilai ekonomis tinggi. Echinodermata mempunyai cara dan kemampuan berbeda dalam menentukan lokasi yang cocok untuk tempat hidupnya, sehingga perbandingan jenis dan kelimpahan Echinodermata di suatu lokasi pada waktu yang berbeda perlu untuk dipelajari. Penelitian ini bertujuan untuk mengetahui struktur komunitas Echinodermata di Pulau Barrang Lompo Kecamatan Ujung Tanah Kota Makassar. Penelitian ini bersifat deskriptif. Pengambilan sampel dilakukan di tiga titik stasiun yaitu stasiun I (Zona lamun), stasiun II (Zona berpasir), dan stasiun III (Zona karang). Penelitian ini dilakukan pada bulan Februari 2016, dengan menggunakan metode transek kuadran. Parameter yang diukur meliputi parameter fisika (temperatur), parameter kimia (pH, salinitas, DO), dan parameter biologi (keanekaragaman dan kelimpahan Echinodermata). Dari hasil penelitian pada tiga tiga titik stasiun ditemukan 11 jenis Echinodermata dalam empat kelas yaitu Asteroidea, Holothuroidea, Echinoidea, dan Ophiuroidea dengan indeks keanekaragaman (H’) tergolong rendah (nilai 0,9), indeks keseragaman populasi (e) rendah (0,3), dan indeks dominansi (C) menunjukkan ada jenis/kelas yang mendominasi (0,5).