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Effect of Gamma Irradiation on Regeneration Capacity of Mangosteen Nodular Callus WARID ALI QOSIM; ROEDHY PURWANTO; GULDOF ALBERT WATTIMENA; WITJAKSONO WITJAKSONO
HAYATI Journal of Biosciences Vol. 14 No. 4 (2007): December 2007
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (37.057 KB) | DOI: 10.4308/hjb.14.4.140

Abstract

The research was conducted to determine the effect of gamma irradiation on regeneration capacity of mangosteed nodular callus. Nodular calli derived from a leaf as explants and cultured on MS medium containing combination of 2.2 µM benzilaminopurin (BAP) and 2.27 µM tidiazuron (TDZ). Nodular calli were irradiated with 0 (control) 5, 10, 15, 20, 25, 30, 35, and 40 Gy doses of gamma irradiation. After the irradiation, the calli were generated on woody plant medium (WPM), supplemented with 1.39 µM polyvinilpirolidon (PVP), 8 g.l-1 agar, 30 g.l-1 sucrose and 2.2 µM BAP concentration. Results showed that the irradiation influence the plant regeneration. Response dose of 50% (RD) that could promote the nodular calli of shoot formation was the 25 Gy while that of the shoot number per nodular calli was the 21 Gy. The shoot number irradiated with total dose 5 Gy (9.1 shoot) was higher than that of 0 Gy (8.6 shoot). Key words: plant regeneration, irradiation gamma rays, mangosteen
Oviposition Deterrent of Bactrocera carambolae Resulted from Eggs Deposition on Mango Muryati Muryati; Y. Andi Trisyono; Witjaksono Witjaksono; Wahyono Wahyono
AGRIVITA, Journal of Agricultural Science Vol 39, No 2 (2017): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v39i2.1097

Abstract

Oviposition deterrent is chemical compounds which are used for avoiding eggs deposition. The oviposition deterrent resulted from eggs deposition is valuable information that can be manipulated for managing its population. The objective of this research was to determine the presence of oviposition deterrent resulted by female Bactrocera carambolae on mango. Extraction of oviposition deterrent was conducted by maceration method. The preference test was performed using two arms olfactometer, meanwhile the oviposition deterrent test was conducted by exposed gravid females to fruit that already smeared with extracts. The result revealed that gravid females of B. carambolae were attracted to methanol extract of 1 day after egg deposition, whereas the methanol extract of 3 and 5 days after egg deposition repelled gravid females. Oviposition deterrent test indicated that methanol extract at category 3 and 5 acted as a deterrent. The preference of gravid females of B. carambolae to different category of infested fruit extracts was probably influenced by the chemicals modification on mango after oviposition. This result suggested that the female B. carambolae do not deposit oviposition deterrent. The phenomenon of deterrence was probably as a result of chemical changes in fruit as a consequence of eggs infestation.
The Use of Non-Military Elements of Statecraft by China during Cambodian Crisis 1997 Witjaksono Witjaksono
Dinamika Global : Jurnal Ilmu Hubungan Internasional Vol 1 No 01 (2016): Dinamika Global : Jurnal Ilmu Hubungan Internasional
Publisher : Universitas Jenderal Ahmad Yani

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (715.142 KB) | DOI: 10.36859/jdg.v1i01.10

Abstract

Rekognisi atas kedaulatan suatu negara merupakan bagian dari kebijakan luar negeri yang dalam decision making process-nya pasti mempertimbangkan cost dan benefit. Proses mempertimbangkan kebijakan luar negeri akan semakin sulit dilakukan apabila rekognisi atas kedaulatan tersebut dilakukan terhadap negara yang sedang mengalami krisis. Saat Kamboja mengalami krisis pada tahun 1997, Tiongkok mengambil langkah strategis dengan memberikan rekognisi terhadap kedaulatan Kamboja. Walaupun Tiongkok memberikan rekognisi dalam bentuk yang tidak empiris, dalam hal ini elemen-elemen non-militer, namun terbukti bahwa bentuk rekognisi semacam itu sudah cukup untuk memberikan dampak berupa keuntungan strategis terhadap kedua negara khususnya Kamboja sebagai negara yang sedang mengalami krisis. Penelitian ini akan menggambarkan best practices dari upaya sebuah negara dalam memberikan rekognisi atas kedaulatan negara lain, melalui elemen-elemen yang bersifat non militer. Pada akhirnya, penggunaan elemen-elemen non-militer merupakan hal yang berpotensi dalam memberikan dampak strategis terhadap rekognisi yang dilakukan bagi negara yang sedang mengalami krisis.