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All Journal PROSIDING SEMINAR NASIONAL MEDIA MEDIKA INDONESIANA Jurnal Kedokteran Diponegoro Medica Hospitalia Prosiding Seminar Nasional Unimus
Edi Dharmana
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Journal : MEDIA MEDIKA INDONESIANA

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Pengaruh Pemberian Ekstrak Phyllanthus niruri Linn Terhadap Infiltrasi Limfosit dan Ekspresi Perforin pada Kanker Kolon Tikus Sprague-Dawley Endang Sawitri; Ign. Riwanto; Tjahjono Tjahjono; Edi Dharmana
MEDIA MEDIKA INDONESIANA 2013:MMI VOLUME 47 ISSUE 1 YEAR 2013
Publisher : MEDIA MEDIKA INDONESIANA

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ABSTRACTThe effect of Phyllanthus niruri Linn extract on lymphocytes infiltration and perforin expression in colon cancer of Sprague-Dawley RatBackground: Colon cancer treatment currently involves immunotherapy that aims to improve the quality of life and survival of patients. Phyllanthus niruri Linn (P. niruri L) may act as an immunomodulator, but its potency in antitumor immune responses has not been revealed. The study was conducted to evaluate the differences between the immunological status of rats suffering colon cancer which were not given to those given the extract of P. niruri L.Methods: The study was randomized posttest-only control group design. Samples were 30 Sprague-Dawley male rats, bodyweight 170-220 gr which induced by 1.2 DMH 30 mg/kgBW subcutaneously. On the weeks 9, 11 and 13, four induced rats each week were sacrified to detect the development of colon cancer. On the weeks of 13th all of 4 rats were developed colon cancer, so the induction were stopped. The rest of 18 induced rats were randomly into two groups: without P.  niruri L or positive control (K+)=9 rats and  given P. niruri L extract 13.5 mg/kg orally or  X  group=9  rats.  After 19th week all of rats were then terminated and tumor lesion of colon were processed hystophatologically. The tissues of colon cancer were stained by H&E for evaluate the lymphocytes infiltration and immunohistochemistry monoclonal antibody anti-perforin for perforin expression. Non pairs t-test was used with considered significant if p<0.05.Results: The mean of lymphocytes infiltration of the group X was 401.89±70.19, it was higher compared to K+ 191.89±50.68 (p=0.000). The mean percentage of perforin expression of group X was 39.00±1.80%, it was higher compared to K+ 23.00± 3.00% (p=0.000). Conclusion: The extract of P. niruri L increases immunologic status through mechanism of lymphocytes infiltration and perforin expression elevation of colon cancer in animal mode.Keywords : Phyllanthus niruri Linn, colon cancer, lymphocytes infiltration, perforinABSTRAKLatar belakang: Penanganan kanker kolon saat ini melibatkan imunoterapi yang bertujuan untuk meningkatkan kualitas hidup dan survival penderitanya. Phyllanthus niruri Linn bekerja sebagai imunomodulator, tetapi potensinya dalam respons imun antitumor belum banyak diungkap. Penelitian bertujuan untuk mengevaluasi perbedaan status imunologis antara tikus coba yang menderita kanker kolon yang tidak diberi dengan yang diberi ekstrak P. niruri L.Metode: Penelitian ini merupakan randomized posttest-only control group design. Sampel berupa 30 ekor tikus Sprague-Dawley jantan, yang diinduksi 1,2 DMH 30 mg/kgBB subkutan sekali setiap minggu. Pada minggu ke-9, 11 dan 13 masing-masing empat ekor tikus dimatikan untuk melihat perkembangan tumor. Minggu ke-13 pada kolon empat tikus telah tumbuh kanker kolon, sehingga induksi dihentikan dan sisa 18 tikus dirandom alokasi menjadi 2 kelompok. Kelompok kontrol positif (K+) tanpa pemberian P. niruri L (9 tikus) dan kelompok diberi P. niruri L. 13,5 mg/kg per hari melalui sonde (9 tikus). Minggu ke-19 semua tikus diterminasi, lesi tumor pada kolon diproses menjadi sediaan histopatologik, kemudian dipulas dengan H&E untuk memeriksa infiltrasi limfosit dan pulasan imunohistokimia menggunakan antibodi monoklonal anti-perforin untuk melihat ekspresi perforin. Analisis data menggunakan uji t tidak berpasangan dengan derajat signifikansi p<0,05.Hasil: Rerata jumlah infiltrasi limfosit kelompok X adalah 401,89±70,19 lebih tinggi dibanding kelompok K+ yaitu191,89±50,68 (p=0,000). Persentase rerata ekspresi perforin kelompok X sebesar 39,00±1,80%, lebih tinggi dibandingkan dengan K+ yakni 23,00±3,00% (p=0,000).Simpulan: Ekstrak P. niruri L meningkatkan status imunologi melalui mekanisme peningkatan infiltrasi limfosit dan ekspresi perforin untuk melawan kanker kolon pada tikus coba
Pengaruh Polifenol Mahkota Dewa Terhadap Proliferasi Sel dan Apoptosis pada Mencit Strain Balb/C yang Diinduksi Benzo(a) Pyrene (BaP) Theopilus W. Watuguly; Indranila KS; Pamela Mercy Papilaya; Edi Dharmana
MEDIA MEDIKA INDONESIANA 2013:MMI VOLUME 47 ISSUE 1 YEAR 2013
Publisher : MEDIA MEDIKA INDONESIANA

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Abstract

ABSTRACT The effect of mahkota dewa polyphenol in the cell proliferation and apoptosis in BaP induced Balb/c ratsBackground: The polyphenol of mahkota dewa (Phaleria macrocarpa [Scheff.] Boerl) has the potency as antioxidant and anticancer which can handle free radicals, but there has not been extensive research on this. This research is aimed to prove the role of mahkota dewa polyphenol in the cell proliferation inhibition and induct lung carcinogenesis apoptosis in strain Balb/c mice which inducted with benzo(a)pyrene (BaP).Methods: Posttest control group design was carried out among 40 strain Balb/c mice sample, aged 1-2 weeks, weighed 20-30 grams, healthy mice condition. All mice were inducted with BaP and then randomized into 2 groups, as control group and the treatment group. The development of the lung tumor was observed by tissue surgery in the 8th, 17th and 26th week. The data collected were AgNORs, and IHC-TUNEL-apoptosis index dying. The data analysis was conducted using Kruskal-Wallis, Mann-Whitney, One-way ANOVA, and Post hoc test LSD with significance degree of p<α (0.05).Results: The oral administration of mahkota dewa polyphenol showed significantly decreasing cell proliferation, increasing apoptosis index in treatment group in week 8, 17 and 26 (p=0.000). Carcinogenesis incidence for the treatment group week 8 and 26 were 2.32±0.26 and 3.93±0.46, while for the treatment group were 1.88±0.38 and 0.88±0.22 (p=0.000). The cell proliferations for control group week 8 and 26 were 1.57±0.12 and 2.29±0.15, while for the treatment group were 1.53±0.11 and 1.60±0.04 (p=0.000). Apoptosis index for the control group for week 8 was 0.00±0.00 and 0.92±0.22 in week 26, while the treatment group was 1.12±0.71 and 2.02±1.05 (p=0.000).Conclusion: The administration of mahkota dewa polyphenol effectively inhibited the cell proliferation activity and increased apoptosis measured by apoptosis index. Therefore polyphenol has anticancer and antioxidant activities which can inhibit lung carcinogenesis in Balb/c mice.Keywords: Polyphenol, mahkota dewa (Phaleria macrocarpa [Scheff.] Boerl), apoptosis index, strain Balb/c mice, benzo(a)pyrene (BaP).ABSTRAKLatar belakang: Polifenol mahkota dewa (Phaleria macrocarpa [Scheff.] Boerl) berpotensi sebagai antioksidan dan antikanker yang mampu menangkap radikal bebas, namun belum banyak diteliti. Penelitian ini bertujuan membuktikan peran polifenol mahkota dewa dalam menginhibisi proliferasi sel dan menginduksi apoptosis pada mencit strain Balb/c hasil induksi Benzo(a)pyrene (BaP).Metode: Posttest control group design dengan sampel 40 mencit strain Balb/c, umur 1-2 minggu, berat 20-30 g, kondisi mencit sehat. Semua mencit diinduksi BaP kemudian hewan dirandomisasi menjadi 2 kelompok yaitu kelompok kontrol dan kelompok perlakuan polifenol 50 mg. Perkembangan tumor paru diamati dengan pembedahan jaringan pada minggu ke-8, 17 dan 26. Data dikumpulkan meliputi AgNORs, pengecatan IHC-TUNEL-indeks apoptosis. Analisis data dengan Kruskal-Wallis, Mann-Whitney, One-way ANOVA, post hoc test LSD dengan derajat kemaknaan p<α (0,05).Hasil: Pemberian oral polifenol mahkota dewa sebesar 50 mg secara bermakna memperlihatkan penurunan insidens karsinogenesis paru, proliferasi sel, protein Bax dan peningkatan indeks apoptosis, protein p53, Bcl-2, ekspresi caspase 3, 8, 9 pada kelompok perlakuan pada minggu ke-8, 17 dan 26 (p=0,000). Insidens karsinogenesis untuk kelompok kontrol minggu ke-8, dan 26 sebesar 2,32±0,26 dan 3,93±0,46, sedangkan kelompok perlakuan sebesar 1,88±0,38 dan 0,88±0,22 (p=0,000). Proliferasi sel untuk kelompok kontrol minggu ke-8 dan 26 sebesar 1,57±0,12 dan 2,29±0,15, sedangkan kelompok perlakuan sebesar 1,53±0,11 dan 1,60±0,04 (p=0,000). Indeks apoptosis pada kelompok kontrol minggu ke-8 sebesar 0,00±0,00 dan 0,92±0,22 minggu ke-26, sedangkan kelompok perlakuan sebesar 1,12±0,71 dan 2,02±1,05 (p=0,000).Simpulan: Pemberian polifenol mahkota dewa efektif meng-inhibisi proliferasi sel dan menginduksi apoptosis melalui peningkatan indeks apoptosis. Jadi polifenol mahkota dewa memiliki aktivitas antikanker dan antioksidan mampu menghambat karsinogenesis paru mencit Balb/c.
Co-Authors Alkhonsa Adibah Darwito - Endang Sawitri Hardhono Susanto Hendra Widjaja Hertanto S.W Ign Riwanto Ign. Riwanto Indranila KS Pamella Mercy Papilaya R. Ngt. Samiasih Rudiyuwono Raharjo Theopilus W Watuguly Tjahjono Tjahjono Yanuarita Tursinawati