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Z. Udin

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Author-ID : 473101

Agriculture, Biological Sciences & Forestry

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Pengaruh Waktu Fertilisasi dan Sistem Inkubasi yang Berbeda terhadap Tingkat Fertilisasi Sapi Lokal Secara In Vitro F. L. Syaiful; R. Saladin; Jaswandi Jaswandi; Z. Udin
Jurnal Peternakan Indonesia (Indonesian Journal of Animal Science) Vol 13, No 1 (2011): Jurnal Peternakan Indonesia
Publisher : Universitas Andalas

The purpose of this study was to determine the effect of fertilization time and different incubation systems on fertilization level by in-vitro. The mature of ovaries from indigenous cow and fresh cement from Holstein Frisian cows (FH), 0.9 % NaCl physiological PBS, Nissui Japan, l,TCM-199, HEPES 30 μM, 20 mL Heparin, 10% goat serum, 250 g/ml FSH BO medium, mediumgentamisain 50 mB-O, mineral oil, alcohol, aquabidest, and 1% aceto orcein were materials and reagents. The Completely Randomized Design (CRD) in factorial pattern was used. Results shown no significant effect on the percentage of fertilized oocytes and level of development of pronuclei (2PN and> 2PN) by different timing of fertilization and incubation system. The development of pronucleus (1PN) showed significant (P <0.05) on 12 hours (37.60 %), but no significant effect on different incubation system. It concluded, the system of incubation and time of fertilization has no effect on oocyte fertilization rate. Oocytes fertilization time can be performed at 6 hours, 12 hours, and 18 hours, while the extension of the period of fertilization until 18 hours did not increase the level of fertilization.

Pengaruh Waktu Pelapisan Spermatozoa Sapi Pada Media TALP yang Disuplementasi bovine serum albumin (BSA) Terhadap Jenis Kelamin Embrio In vitro T. Afriani; Z. Udin; Jaswandi Jaswandi; S. Asmairicen
Jurnal Peternakan Indonesia (Indonesian Journal of Animal Science) Vol 13, No 2 (2011): Jurnal Peternakan Indonesia
Publisher : Universitas Andalas

This research aim to know the influence of depth veneering time of spermatozoa at media of TALP which was supplemented by 4% BSA to embryo gender in in- vitro and to get the time dissociation of spermatozoa which have the best chromosome Y. This research represent attempt of exsperiment in RBT by 4 time veneering of spermatozoa as treatment, times intake of semen of epididymis to groups. Variables measure are: 1). mortality of spermatozoa, 2). number of in vitro fertilzation, 3. masculine gender embryo ratio. Result shown, the veneering of cow spermatozoa during 90 minute in media of TALP which was supplemented by 4% bovine serum albumin (BSA) can dissociate spermatozoa which have chromosome Y with percentage of highest male sex (90.00±24.49), and highest percentage of mortality and fertility were obtained at veneering during 60 minute with scoring 73.33 ± 5.16 and 53.92±18.79 respectively.

Pengaruh Penambahan Follicle Stimulatins Hormone (FSH) dan Pregnant Mare's Serum Gonadrotropin (PMSG) dalam Sel Granulosa Terhadap Konsentrasi Progesteron pada Tingkat Maturasi Oosit D. Dianti; Z. Udin; Jaswandi Jaswandi
Jurnal Peternakan Indonesia (Indonesian Journal of Animal Science) Vol 13, No 1 (2011): Jurnal Peternakan Indonesia
Publisher : Universitas Andalas

This research was conducted to determine the effect of adding hormone FSH and PMSG in vitro oocyte maturation medium on maturation rate and hormone progeiterone. In this experiment, oocyte derived from ovaries of slaughtered cattle at the slaughterhouse. Maturation medium using TCM-199 supplemented serum 10%, gentamicin 50ug/ml, FSH I0g/mlor PMSG 10 g/ml and the culture of granulosa cells lxl05 cells/ml. In each treatment unit is used 20 oocyt quality A and B in 100 mL of maturation medium. Randomized Blok Design in factorial (2x2x4) was used in this experiment. The Factor A culture (without cells and granulosa cell), B factor hormones (PMSG andFSH). Variables measured were the number maturation and progesterone hormone level in maturation medium by using RIA techniques. The results shown, there is no interaction of hormone addition in granulosa cell numbers on maturation level and progesterone concentration in maturation medium. Granulosa cell in maturation medium significantly (P<0.05) increased the number of oocyte maturation in vitro. The highest maturation was obtained from media with granulosa cells and FSH of 71.25%, while the highest progesterone obtained from media with granulosa cells and PMSG 1.40 ng / ml.

Potensi dan Tingkat Kematangan In vitro Oosit Sapi Peranakan Simmental Jaswandi Jaswandi; D. Mardona; F. Arlina; Z. Udin
Jurnal Peternakan Indonesia (Indonesian Journal of Animal Science) Vol 12, No 3 (2007): Jurnal Peternakan Indonesia
Publisher : Universitas Andalas

The present research was conducted to investigate the potency and viabitity of oocytes of Simmental cattle for production of embryo in in-vitro method. Oacytes were collected from the ovaries of slaughtered Simmental cattle. At first, the ovaries were cleaned and measured for weigh, length, diameter and thickness. The ovaries were then sliced to collect oocyte follicles. The collected oocytes which were devided into 3 groups based on length size (<2, 2-6 and >6 mm) were kept in ringer solution (0,9 % Nacl) by temperature of 35oC. By using microscope the physical performances of the oocytes were observed and then classified into 3 different qualities of A, B and C/D. The oocytes of A ond B quality were kept in TCM-199 medium for maturity process. Their viability was described as percentage af oocytes which reach metaphase II (M-II). stage during the proces of maturity. The data was statistically analyzed by t-test. Results of the experiment showed that the physical measurement af ovaries were found significant diferent (P>0,05) between the left and right sides, but the potency of follicles from the both sides were not significant different (P>0,05). The percentage of oocytes reach the metaphase II stage was about 51,28 s/d 13,65 %. It was concluded that Simmental has a high the potency and viability oocytes for embryos production in vitro.

Co-Authors D. Dianti D. Mardona F. Arlina F. L. Syaiful Jaswandi . R. Saladin S. Asmairicen Tinda Afriani

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