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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Kebun Raya
Elizabeth Handini
Pusat Konservasi Tumbuhan Kebun Raya, Lembaga Ilmu Pengetahuan Indonesia
Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance

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PENGARUH PENAMBAHAN BERBAGAI KADAR PISANG DAN UBI JALAR PADA PERTUMBUHAN KULTUR TIGA JENIS PHALAENOPSIS Garvita, Raden Vitri; Handini, Elizabeth
Buletin Kebun Raya Vol 14, No 2 (2011): Buletin Kebun Raya Vol. 14 (2) Juli 2011
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Abstract

The aim of the experiment was to determine the effect of organic materials on the growth of Phalaenopsis zebrina, P.  javanica and P.  fuscata explants without roots  in Knudson’s C medium with additional of coconut water.  Observation were conducted for 10 months and the data were analyzed by ANOVA. The result showed that the survival ability of P. zebrina and P. javanica were only 3 months in KC medium with additional of coconut water (150 g/l), banana (50 g/l) and sweet potatoes (20 g/l), while P. fuscata grew further up to 10 months in all media. Additional of organic material: 150 g/l coconut water, 25 g/l banana and 15 g/l sweet potatoes in KC medium gave the best result on increasing the leaf and the shoot numbers, while additional of 150 g/l coconut water, 50 g/l banana and 20 g/l sweet potatoes to KC medium only increasing the  root number of P. fuscata.
MIKROPROPAGASI ANGGREK ALMA Grammatophyllum scriptum Blume Handini, Elizabeth
Buletin Kebun Raya Vol 11, No 1 (2008): Buletin Kebun Raya Vol. 11 (1) Januari 2008
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Abstract

Grammatophyllum scriptum is a beautiful wild orchid that has an interesting economic value. However, the existence of this ornamental plant species is at risk because of irresponsible exploitation of the natural population and insufficient cultivation efforts. Currently, the Bogor Botanic Garden is developing a program of orchid propagation for enhancing the conservation of all potential orchid collections. The propagation of G. scriptum was basically carried out by culturing the seeds in a modified Hyponex or Vacin & Went medium, transplanting the plantlets into a more suitable medium and finally acclimatizing the resulting seedlings. A study was done to observe the effect of fertilizer application on the growth of acclimatized G. scriptum seedlings, as a means to improve the propagation technique of the species. The result showed that the application of 1 and 2 m1/I organic fertilizer (Sugih) as well as 1 and 2 g/I inorganic fertilizer (Hyponex) increased the seedling mortality, in which the application of 2 g/ml Hyponex fertilizer was being the most damaging treatment, leaving 12,5 % seedlings to survive. However, in terms of seedling growth, the application of 1 m1/I Sugih fertilizer was slightly beneficial to the formation of root and the elongation of stem of the acclimatized seedlings. It may be suggested, therefore, that G. scriptum seedlings require no (or very little amount of) supplementary fertilizer to boost their vegetative growth.
PERKECAMBAHAN BIJI KANTONG SEMAR (Nepenthes gracilis Korth.) SECARA IN VITRO Isnaini, Yupi; Handini, Elizabeth
Buletin Kebun Raya Vol 10, No 2 (2007): Buletin Kebun Raya Vol. 10 (2) Juli 2007
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Abstract

Mature seeds of a pitcher plant (Nepenthes grocilis Korth.) were cultured in seven different media, namely half strength MS medium, half strength MS medium supplemented with BAP and NAA, 0.8 KC medium supplemented with coconut water + bean sprout extract, 0.8 KC + H3B03 + CuSO3 + ZnSO4 + coconut water + bean sprout extract, 0.25 KC + Vitamin (MS), HYPONeX + coconut water + sprout bean extract, and HYPONeX + potato extract. Each culture was incubated under light conditions. Young seedlings were subsequently transferred onto fresh media of 0.5 MS and 0.25 KC supplemented with 0, 5, 10, and 15 mg/I GA3 after 15 weeks of sowing. Seed germination and seedling growth were observed weekly. It was noted that the seeds started to germinate in ten weeks after incubation and the highest seed germination was obtained in HYPONeX medium + potato extract. However, the appearance of the seedlings was better on MS media and KC media without organic compound than on the other media. Seedlings in all level of GA3 in half strength MS media and 0.25 KC media grew faster than those in media without GA3.
PENGARUH PENAMBAHAN BERBAGAI KADAR PISANG DAN UBI JALAR PADA PERTUMBUHAN KULTUR TIGA JENIS PHALAENOPSIS Garvita, Raden Vitri; Handini, Elizabeth
Buletin Kebun Raya Vol 14, No 2 (2011): Buletin Kebun Raya Vol. 14 (2) July 2011
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The aim of the experiment was to determine the effect of organic materials on the growth of Phalaenopsis zebrina, P.  javanica and P.  fuscata explants without roots  in Knudson’s C medium with additional of coconut water.  Observation were conducted for 10 months and the data were analyzed by ANOVA. The result showed that the survival ability of P. zebrina and P. javanica were only 3 months in KC medium with additional of coconut water (150 g/l), banana (50 g/l) and sweet potatoes (20 g/l), while P. fuscata grew further up to 10 months in all media. Additional of organic material: 150 g/l coconut water, 25 g/l banana and 15 g/l sweet potatoes in KC medium gave the best result on increasing the leaf and the shoot numbers, while additional of 150 g/l coconut water, 50 g/l banana and 20 g/l sweet potatoes to KC medium only increasing the  root number of P. fuscata.
MIKROPROPAGASI ANGGREK ALMA Grammatophyllum scriptum Blume Handini, Elizabeth
Buletin Kebun Raya Vol 11, No 1 (2008): Buletin Kebun Raya Indonesia Vol. 11 (1) January 2008
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Grammatophyllum scriptum is a beautiful wild orchid that has an interesting economic value. However, the existence of this ornamental plant species is at risk because of irresponsible exploitation of the natural population and insufficient cultivation efforts. Currently, the Bogor Botanic Garden is developing a program of orchid propagation for enhancing the conservation of all potential orchid collections. The propagation of G. scriptum was basically carried out by culturing the seeds in a modified Hyponex or Vacin & Went medium, transplanting the plantlets into a more suitable medium and finally acclimatizing the resulting seedlings. A study was done to observe the effect of fertilizer application on the growth of acclimatized G. scriptum seedlings, as a means to improve the propagation technique of the species. The result showed that the application of 1 and 2 m1/I organic fertilizer (Sugih) as well as 1 and 2 g/I inorganic fertilizer (Hyponex) increased the seedling mortality, in which the application of 2 g/ml Hyponex fertilizer was being the most damaging treatment, leaving 12,5 % seedlings to survive. However, in terms of seedling growth, the application of 1 m1/I Sugih fertilizer was slightly beneficial to the formation of root and the elongation of stem of the acclimatized seedlings. It may be suggested, therefore, that G. scriptum seedlings require no (or very little amount of) supplementary fertilizer to boost their vegetative growth.
PERKECAMBAHAN BIJI KANTONG SEMAR (Nepenthes gracilis Korth.) SECARA IN VITRO Isnaini, Yupi; Handini, Elizabeth
Buletin Kebun Raya Vol 10, No 2 (2007): Buletin Kebun Raya Indonesia Vol. 10 (2) July 2007
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Mature seeds of a pitcher plant (Nepenthes grocilis Korth.) were cultured in seven different media, namely half strength MS medium, half strength MS medium supplemented with BAP and NAA, 0.8 KC medium supplemented with coconut water + bean sprout extract, 0.8 KC + H3B03 + CuSO3 + ZnSO4 + coconut water + bean sprout extract, 0.25 KC + Vitamin (MS), HYPONeX + coconut water + sprout bean extract, and HYPONeX + potato extract. Each culture was incubated under light conditions. Young seedlings were subsequently transferred onto fresh media of 0.5 MS and 0.25 KC supplemented with 0, 5, 10, and 15 mg/I GA3 after 15 weeks of sowing. Seed germination and seedling growth were observed weekly. It was noted that the seeds started to germinate in ten weeks after incubation and the highest seed germination was obtained in HYPONeX medium + potato extract. However, the appearance of the seedlings was better on MS media and KC media without organic compound than on the other media. Seedlings in all level of GA3 in half strength MS media and 0.25 KC media grew faster than those in media without GA3.
PENYIMPANAN BIJI ANGGREK Coelogyne spp. UNTUK KONSERVASI EX SITU Puspitaningtyas, Dwi Murti; Handini, Elizabeth
Buletin Kebun Raya Vol 17, No 2 (2014): Buletin Kebun Raya Vol. 17 (2) July 2014
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Abstract

Coelogyne merupakan anggrek asli Indonesia yang mempunyai ukuran bunga relatif besar dan warna menarik. Penyimpanan biji Coelogyne perlu dilakukan untuk menjaga keragaman spesies tersebut. Percobaan penyimpanan biji pada tujuh spesies anggrek Coelogyne pada suhu -20oC telah dilakukan selama empat tahun di PKT KR–LIPI. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap, dengan faktor media yang diperlakukan pada masing-masing spesies Coelogyne. Empat macam media kultur yang digunakan untuk menguji perkecambahan biji adalah KCA (Knudson C), KC (modifikasi Knudson C), VW (modifikasi Vacin and Went), dan HS (modifikasi Hyponex).  Uji viabilitas biji diamati setelah biji disimpan selama 0, 1, 2, 3, 6, 9, 12, 18, 24, 36, 48 bulan. Hasil penelitian menunjukkan bahwa biji Coelogyne spp. mampu berkecambah di  empat media percobaan. Viabilitas  biji Coelogyne spp. menurun dalam waktu tiga bulan, enam bulan, sembilan bulan, dan seterusnya tergantung pada spesies Coelogyne tersebut.  Biji Coelogyne yang dapat disimpan selama 1–2 tahun adalah C. pandurata, C. asperata dan C. rumphii, sedangkan biji yang dapat disimpan selama tiga tahun adalah biji C. foerstermannii dan C. pulverula.  Biji C.rochussenii dan C. celebensis dengan masa hidup yang singkat harus ditanam segera setelah panen, dan selanjutnya disimpan dalam bentuk kultur bibit in vitro atau biji sintetis melalui enkapsulasi protocorm.   
Radiosensitivitas dan Pertumbuhan In Vitro Protocorm Anggrek ‘Tien Soeharto’ (Cymbidium hartinahianum J.B. Comber & R.E. Nasution) Sudarsono; Handini, Elizabeth; Sukma, Dewi; Roostika, Ika
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 48 No. 3 (2020): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (563.81 KB) | DOI: 10.24831/jai.v48i3.29170

Abstract

C. hartinahianum J.B. Comber & R.E. Nasution merupakan salah satu jenis anggrek endemik di Sumatera Utara. Habitatnya terbatas di dataran tinggi dengan suhu rendah. Habitat alami tersebut sudah banyak dikonversi untuk penggunaan lainnya. Induksi radiasi sinar gamma merupakan metode alternatif untuk mendapatkan tanaman mutan toleransi terhadap kondisi suhu yang tinggi. Penelitian ini bertujuan untuk mengetahui radiosensitivitas protocorm C. hartinahianum J.B. Comber & R.E. Nasution terhadap iradiasi sinar gamma dan pertumbuhan PLBs di suhu ruang kultur yang berbeda. Protocorm diiradiasi dalam kisaran dosis iradiasi 0, 5, 10, 15, dan 20 Gy, kemudian separoh populasi protocorm pasca iradiasi diinkubasi dalam ruang kultur dengan suhu 16-18 oC dan separoh yang lain di suhu 22-27 oC, untuk mengevaluasi radiosentitivitas dan pertumbuhannya. Hasil penelitian menunjukkan bahwa LD20 dan LD50 protocorm di ruang kultur bersuhu 16-18 oC adalah 26.98 Gy dan 38.24, sementara LD20 dan LD50 protocorm pada ruang kultur bersuhu 22-27 oC adalah 17.08 Gy dan 27.29 Gy. Proliferasi PLBs dan morfogenesis tunas C. hartinahianum lebih baik pada ruang kultur dengan suhu 22-27 oC. Hasil penelitian menunjukkan bahwa re-iradiasi sinar gamma sebaiknya dilakukan pada LD20 atau LD50 dari ruang kultur dengan suhu 22-27 oC yaitu sebesar 17.08 Gy dan 27.29 Gy, untuk mendapatkan mutan putatif C. hartinahianum. Kata kunci: dosis letal, induksi mutasi in vitro, iradiasi sinar gamma, multiplikasi, protocorm like bodies (PLBs)
Co-Authors Dewi Sukma Dwi Murti Puspitaningtyas, Dwi Murti Ika Roostika Raden Vitri Garvita Sudarsono Yupi Isnaini, Yupi