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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Buletin Kebun Raya BACA: Jurnal Dokumentasi dan Informasi
Dwi Murti Puspitaningtyas, Dwi Murti
Pusat Konservasi Tumbuhan Kebun Raya, Lembaga Ilmu Pengetahuan Indonesia
Agriculture, Biological Sciences & Forestry Environmental Science Library & Information Science

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PENGARUH IRADIASI SINAR GAMMA TERHADAP VARIASI PERTUMBUHAN ANGGREK Brachypeza indusiata (Reichb.f) Garay SECARA IN VITRO Wardhani, Maria Utami Dewi; Puspitaningtyas, Dwi Murti; Dinarti, Diny
Buletin Kebun Raya Vol 10, No 2 (2007): Buletin Kebun Raya Vol. 10 (2) Juli 2007
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Abstract

A research was carried out in Orchid Laboratory of Bogor Botanical Gardens to observe the effect of gamma ray irradiation on the growth variation of Brachypezo indusiata (Reichb.f) Garray and suitable culture medium for propagating the plant. The research was arranged in a factorial experiment with a Completely Randomized Design. The first factor was gamma ray irradiation on protocorm-like­bodies (plb) of germinated seeds of B. indusiata in which four levels of irradiation was applied, i.e. 0, 10, 20, and 30 Gy. The second factor was the use of two different culture media, i.e. Vacin & Went medium supplemented with either banana pulp or a mixture of tomato and sprout bean as the essential organic compound of the medium. The result showed that the irradiation treatment improved the survival of B. indusiata explants, most significantly at 20 Gy level of irradiation, and could modify the growth of the resulted plantlets by reducing the height of the plant and the length and width of the leaves. The higher the level of irradiation the smaller the plantlets produced. Meanwhile, the addition of organic compound derived from banana fruit into the basal medium of Vacin & Went enhanced the growth of the plantlets better than that derived from a mixture of tomato fruit and bean sprout. However, there was no significant interaction effect detected between the irradiation treatments and the use of different culture media.
PENGARUH IRADIASI SINAR GAMMA TERHADAP VARIASI PERTUMBUHAN ANGGREK Brachypeza indusiata (Reichb.f) Garay SECARA IN VITRO Wardhani, Maria Utami Dewi; Puspitaningtyas, Dwi Murti; Dinarti, Diny
Buletin Kebun Raya Vol 10, No 2 (2007): Buletin Kebun Raya Indonesia Vol. 10 (2) July 2007
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Abstract

A research was carried out in Orchid Laboratory of Bogor Botanical Gardens to observe the effect of gamma ray irradiation on the growth variation of Brachypezo indusiata (Reichb.f) Garray and suitable culture medium for propagating the plant. The research was arranged in a factorial experiment with a Completely Randomized Design. The first factor was gamma ray irradiation on protocorm-like­bodies (plb) of germinated seeds of B. indusiata in which four levels of irradiation was applied, i.e. 0, 10, 20, and 30 Gy. The second factor was the use of two different culture media, i.e. Vacin & Went medium supplemented with either banana pulp or a mixture of tomato and sprout bean as the essential organic compound of the medium. The result showed that the irradiation treatment improved the survival of B. indusiata explants, most significantly at 20 Gy level of irradiation, and could modify the growth of the resulted plantlets by reducing the height of the plant and the length and width of the leaves. The higher the level of irradiation the smaller the plantlets produced. Meanwhile, the addition of organic compound derived from banana fruit into the basal medium of Vacin & Went enhanced the growth of the plantlets better than that derived from a mixture of tomato fruit and bean sprout. However, there was no significant interaction effect detected between the irradiation treatments and the use of different culture media.
PENYIMPANAN BIJI ANGGREK Coelogyne spp. UNTUK KONSERVASI EX SITU Puspitaningtyas, Dwi Murti; Handini, Elizabeth
Buletin Kebun Raya Vol 17, No 2 (2014): Buletin Kebun Raya Vol. 17 (2) July 2014
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Abstract

Coelogyne merupakan anggrek asli Indonesia yang mempunyai ukuran bunga relatif besar dan warna menarik. Penyimpanan biji Coelogyne perlu dilakukan untuk menjaga keragaman spesies tersebut. Percobaan penyimpanan biji pada tujuh spesies anggrek Coelogyne pada suhu -20oC telah dilakukan selama empat tahun di PKT KR–LIPI. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap, dengan faktor media yang diperlakukan pada masing-masing spesies Coelogyne. Empat macam media kultur yang digunakan untuk menguji perkecambahan biji adalah KCA (Knudson C), KC (modifikasi Knudson C), VW (modifikasi Vacin and Went), dan HS (modifikasi Hyponex).  Uji viabilitas biji diamati setelah biji disimpan selama 0, 1, 2, 3, 6, 9, 12, 18, 24, 36, 48 bulan. Hasil penelitian menunjukkan bahwa biji Coelogyne spp. mampu berkecambah di  empat media percobaan. Viabilitas  biji Coelogyne spp. menurun dalam waktu tiga bulan, enam bulan, sembilan bulan, dan seterusnya tergantung pada spesies Coelogyne tersebut.  Biji Coelogyne yang dapat disimpan selama 1–2 tahun adalah C. pandurata, C. asperata dan C. rumphii, sedangkan biji yang dapat disimpan selama tiga tahun adalah biji C. foerstermannii dan C. pulverula.  Biji C.rochussenii dan C. celebensis dengan masa hidup yang singkat harus ditanam segera setelah panen, dan selanjutnya disimpan dalam bentuk kultur bibit in vitro atau biji sintetis melalui enkapsulasi protocorm.   
Population Study of the Golden Chicken Fern (Cibotium barometz (L.) J. Sm. in Riau Province, Sumatra Praptosuwiryo, Titien Ngatinem; Puspitaningtyas, Dwi Murti; Pribadi, Didit Okta; Rugayah, Rugayah
Journal of Tropical Life Science Vol 7, No 2 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.02.13

Abstract

Cibotium barometz (L.) J.Sm. (Cibotiaceae) is an important export commodity for both traditional and mod-ern medicine. Populations of this species in several countries have decreased rapidly due to the uncontrolled collection of the rhizome parts for medicinal purposes. Since 1976, this species has been included in Appendix II of the Convention on International Trade in Endangered Species (CITES). This means that no export is allowed without a prior permit issued by the CITES committee.  In order to utilize an endangered species sustainably, the global NDF (Non-Detriments Finding) system is applied for determining annual quotas. Therefore, monitoring and updating the inventory of C. barometz in its natural habitat should be carried out annually. A population study of C. barometz carried out in 2011 in Riau Province, Sumatra, is reported here. The aims of the study were: 1) to inventory C. barometz and determine its variation in Riau Province, Suma-tra, 2) to study the distribution and ecology of C. barometz, and 3) to assess the population size of this spe-cies by using random search methodology incorporating belt line transects. Two variants of C. barometz are recognized; they are the golden yellow and golden brown variants. C. barometz is distributed in eight loca-tions of Kampar District of Riau Province, in the secondary forest and rubber agroforest between 80 m and 600 m above sea level (asl). This species grows well in open to partially opened areas of secondary forest and rubber plantation in hills with a range of slope between 30° and 90°, with a relatively high humidity, 60 – 90%, in acid to nearly neutral soil, with a range of soil fertility from very poor to very humus rich soil.  The average population density determined in our study was 20 plants per 100 square meter. The highest popula-tion size was in the secondary forest of Bukit Kuda Beban at 590 – 600 m asl., viz. 9405 plants with a popula-tion density  of 47 plants per 100 square meter.
Dataset koleksi anggrek: Eksplorasi flora Samosir, Sumatera Utara Suminarsih, Eka Meifrina; Puspitaningtyas, Dwi Murti
BACA: Jurnal Dokumentasi dan Informasi 2024: SPECIAL ISSUE - DATA IN BRIEF FOR REPOSITORI ILMIAH NASIONAL
Publisher : Direktorat Repositori, Multimedia, dan Penerbitan Ilmiah - Badan Riset dan Inovasi Nasional (BRIN Publishing)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/baca.2024.7776

Abstract

Sumatra Island, with its tropical climate, provides an ideal habitat for orchid diversity. However, the rich diversity and potential of these orchids are increasingly threatened by high deforestation rates across Indonesia, leading to risks of reduction and even extinction. To address this, conservation activities are underway at the Samosir Botanic Garden. This study aimed to inventory the orchid diversity on Samosir Island. Collected orchids are being conserved as a living collection both ex situ at the Bogor Botanic Garden and in situ at the Samosir Botanic Garden. An exploratory method was used, resulting in a collection of 80 orchid specimens, representing 35 genera, 80 species, and 313 individual samples. These orchids were found at altitudes ranging from 989 to 1,799 meters above sea level and included 60 epiphytic species, 1 lithophytic species, and 19 terrestrial species. A total of 23 orchid species were identified during the exploration, and data on these species are stored in the National Scientific Repository (RIN). This repository provides a valuable resource for further research on the orchid species richness in Samosir Regency.
Co-Authors Didit Okta Pribadi Diny Dinarti Elizabeth Handini Maria Utami Dewi Wardhani, Maria Utami Dewi Rugayah Rugayah Suminarsih, Eka Meifrina Titien Ngatinem Praptosuwiryo