Siti Nuramaliati Prijono, Siti Nuramaliati
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Journal : JURNAL BIOLOGI INDONESIA

Kajian Pemberian Pakan Alternatif terhadap Konsumsi, Kecernaan, dan Efisiensi Penggunaan Pakan pada Jelarang Paha Putih (Ratufa Affinis Raffles, 1821) Farida, Wartika Rosa; Prijono, Siti Nuramaliati
JURNAL BIOLOGI INDONESIA Vol 9, No 2 (2013): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v9i2.170

Abstract

Four cream-coloured giant squirrel (Ratufa affinis) consisted of one male and three females used in this study to determinethe effect of feeding alternatives on consumption, digestibility, and feed efficiency use. During the studyeach of the animals was placed in individual cages equipped with sleeping box. Feed given were consisting of Guava(Psidium guajava), sweet corn (Zea mays), coconut (Cocos nucifera), peanut (Arachis hypogea), cucumber (Cucumissativus), mung bean sprouts (Vigna radiata), and sunflower seed (Helianthus annuus). Feedstuffs are given based onthis animal preferences on the grain in its natural habitat. Feed given cafetaria and drinking water available ad libitum.Results showed that the average of dry matter consumed by male was 45.95 g / head / day and by female 39.14g / head / day; rough protein by male 6.99 g / head / day and by female 5.76 g / head / day ; gross energy by male2392 cal / head / day and by female 2116 cal / head / day. Feed efficiency use of male is 13,99% and female is12,63%. The average of body weight gain of the male animal is higher than that of the female, namely 6.43 g / head /day and 4.88 g / head / day respectively. The average value of digested organic matter or Total Digestible Nutrient(TDN) was higher in the female animal, namely 95.41%, than that of the male, namely 92.68%; as well as digestedenergy or Digestible Energy (DE) in the female animal was higher than that in the male, namely 93.60% and91.17%, respectively.
Divergensi DNA Mitokondria pada Burung Pijantung Kecil (Arachnothera longirostra) dari Indonesia Prijono, Siti Nuramaliati; Irham, Mohammad; Astuti, Dwi
JURNAL BIOLOGI INDONESIA Vol 13, No 2 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2130.881 KB) | DOI: 10.14203/jbi.v13i2.3394

Abstract

ABSTRACTA total of 781 base pairs of mitochondrial DNA ND2 gene was analyzed to investigate their divergence in the Arachnothera longirostra (Nectariniidae) birds in Jawa and Sumatera islands of Indonesia. Blood and tissue samples were collected from 27 birds consisted of 8 samples from T.N.G. Halimun, 6 samples from T.N.G. Gede Pangrango, 6 samples from T.N. Ujung Kulon, 1 sample from Dieng (Jawa), and 6 samples from Jambi (Sumatera). Total genomic DNA was extracted from each sample and used in PCR to amplify a single fragment of mDNA ND2 gene. Their DNA sequence data were compared with those from Johor (Malaya Peninsula) and Sabah-Sarawak Borneo (GenBank). There were 61 variable sites included 34 parsimony sites and 27 sequence haplotypes. Mean genetic distance or sequence divergences within bird populasions were ranged from the lowest (0.25 %) for Ujung Kulon to the highest (0.76 %) for G. Gede Pangrango; while mean genetic distance between populations were of 1.67% to 2.82 % for Java island vs Jambi (Sumatera), and 1.06 to 2.09 % for Java vs Borneo. Phylogenetic NJ tree showed that there were two main clades, i.e. 1) birds of Jambi (Sumatra), Johor (Malay Peninsula), Sabah-Sarawak (Borneo), and Ujung Kulon (Java), and 2) birds of G. Gede Pangrango, G. Halimun, and Dieng (Java). Past geologic event may influenced genetic divergence occurred within the populations. Moreover, geographic features such as mountain may played role for population isolation as showed on the Little Spiderhunter bird from G. Halimun, G. Gede Pangrango and Dieng.Key words: mitochondrial DNA, ND2 gene, Arachnothera longirostra, genetic divergence
KAJIAN PEMBERIAN PAKAN ALTERNATIF TERHADAP KONSUMSI, KECERNAAN, DAN EFISIENSI PENGGUNAAN PAKAN PADA JELARANG PAHA PUTIH (RATUFA AFFINIS RAFFLES, 1821) Farida, Wartika Rosa; Prijono, Siti Nuramaliati
JURNAL BIOLOGI INDONESIA Vol 9, No 2 (2013): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v9i2.170

Abstract

Four cream-coloured giant squirrel (Ratufa affinis) consisted of one male and three females used in this study to determinethe effect of feeding alternatives on consumption, digestibility, and feed efficiency use. During the studyeach of the animals was placed in individual cages equipped with sleeping box. Feed given were consisting of Guava(Psidium guajava), sweet corn (Zea mays), coconut (Cocos nucifera), peanut (Arachis hypogea), cucumber (Cucumissativus), mung bean sprouts (Vigna radiata), and sunflower seed (Helianthus annuus). Feedstuffs are given based onthis animal preferences on the grain in its natural habitat. Feed given cafetaria and drinking water available ad libitum.Results showed that the average of dry matter consumed by male was 45.95 g / head / day and by female 39.14g / head / day; rough protein by male 6.99 g / head / day and by female 5.76 g / head / day ; gross energy by male2392 cal / head / day and by female 2116 cal / head / day. Feed efficiency use of male is 13,99% and female is12,63%. The average of body weight gain of the male animal is higher than that of the female, namely 6.43 g / head /day and 4.88 g / head / day respectively. The average value of digested organic matter or Total Digestible Nutrient(TDN) was higher in the female animal, namely 95.41%, than that of the male, namely 92.68%; as well as digestedenergy or Digestible Energy (DE) in the female animal was higher than that in the male, namely 93.60% and91.17%, respectively.
DIVERGENSI DNA MITOKONDRIA PADA BURUNG PIJANTUNG KECIL (ARACHNOTHERA LONGIROSTRA) DARI INDONESIA Prijono, Siti Nuramaliati; Irham, Mohammad; Astuti, Dwi
JURNAL BIOLOGI INDONESIA Vol 13, No 2 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v13i2.3394

Abstract

ABSTRACTA total of 781 base pairs of mitochondrial DNA ND2 gene was analyzed to investigate their divergence in the Arachnothera longirostra (Nectariniidae) birds in Jawa and Sumatera islands of Indonesia. Blood and tissue samples were collected from 27 birds consisted of 8 samples from T.N.G. Halimun, 6 samples from T.N.G. Gede Pangrango, 6 samples from T.N. Ujung Kulon, 1 sample from Dieng (Jawa), and 6 samples from Jambi (Sumatera). Total genomic DNA was extracted from each sample and used in PCR to amplify a single fragment of mDNA ND2 gene. Their DNA sequence data were compared with those from Johor (Malaya Peninsula) and Sabah-Sarawak Borneo (GenBank). There were 61 variable sites included 34 parsimony sites and 27 sequence haplotypes. Mean genetic distance or sequence divergences within bird populasions were ranged from the lowest (0.25 %) for Ujung Kulon to the highest (0.76 %) for G. Gede Pangrango; while mean genetic distance between populations were of 1.67% to 2.82 % for Java island vs Jambi (Sumatera), and 1.06 to 2.09 % for Java vs Borneo. Phylogenetic NJ tree showed that there were two main clades, i.e. 1) birds of Jambi (Sumatra), Johor (Malay Peninsula), Sabah-Sarawak (Borneo), and Ujung Kulon (Java), and 2) birds of G. Gede Pangrango, G. Halimun, and Dieng (Java). Past geologic event may influenced genetic divergence occurred within the populations. Moreover, geographic features such as mountain may played role for population isolation as showed on the Little Spiderhunter bird from G. Halimun, G. Gede Pangrango and Dieng.Key words: mitochondrial DNA, ND2 gene, Arachnothera longirostra, genetic divergence