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All Journal Agrosains: Jurnal Penelitian Agronomi Asian Journal of Agriculture Agrotechnology Research Journal PRIMA: Journal of Community Empowering and Services Journal of Biodiversity and Biotechnology
Puji Harsono, Puji
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Agriculture, Biological Sciences & Forestry Environmental Science

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Rice response to cadmium bioremediation using sorghum and mycorrhiza HARSONO, PUJI; HASANAH, NUR AZIZAH USWATUN; PURWANTO, EDI; SAMANHUDI, SAMANHUDI
Asian Journal of Agriculture Vol. 9 No. 2 (2025)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/asianjagric/g090219

Abstract

Abstract. Harsono P, Hasanah NAU, Purwanto E, Samanhudi. 2025. Rice response to cadmium bioremediation using sorghum and mycorrhiza. Asian J Agric 9: 522-532. Cadmium (Cd) contamination at 2.9 mg kg-¹ in paddy soils reduces rice productivity and threatens food safety. This study aimed to evaluate the effectiveness of sorghum (Sorghum bicolor) phytoremediation combined with Arbuscular Mycorrhizal Fungi (AMF) in improving rice growth under mild Cd contamination. A factorial randomized complete block design with two cropping systems (seed vs. ratoon, where the same crop is re-grown from the stubble or roots of the first crop) and seven remediation treatments (control; three sorghum varieties (Super 1, Samurai 1, Kawali) combine with/without 10 g AMF plot-¹) was applied in 42 plots (3×4 m). Rice cv. IR 64 was transplanted 30 days after remediation. Significant interactions (p<0.05) between cropping system and remediation treatment occurred for plant height and leaf area. The Kawali + AMF treatment produced the most significant gains relative to the control: plant height +7.7%, leaf area +207%, chlorophyll +236% and total dry biomass +152%, while extending the vegetative phase by 23%. Improvements were consistent across sorghum seed and sorghum ratoon systems, indicating that remediation benefits persist beyond the first harvest. Ratoon sorghum-maintained remediation benefits, with 7-10% taller plants and 8% greater leaf area than the control, despite slightly reduced canopy size compared with seed sorghum; all sorghum treatments delayed flowering by ~11 days, indicating reduced Cd stress. High-biomass sorghum, especially Kawali, combined with AMF, enhances rice growth and physiology while stabilizing Cd, providing a scalable remediation strategy for sustainable rice production in Cd-contaminated tropical agroecosystems.
The Effect of Growth Regulator Formulation and Boron on The Growth and Yield of Shallot Varieties Harsono, Puji; Putri, Ade Widya; Haryanto, Eddy Tri; Handoyo, Gani Cahyo
Agrosains : Jurnal Penelitian Agronomi Vol 27, No 1 (2025): Agrosains: Jurnal Penelitian Agronomi
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agsjpa.v27i1.100603

Abstract

Regulators (PGRs) are used to stimulate the development and growth of bulbs in order to produce a large number of bulbs. This study aims to identify the effect of PGRs and boron formulations that can increase the growth and yield of several variety of shallots. This research took place in the cultivation field of Ngringo Village, Jaten District, Karanganyar Regency from April-August 2023. The method used was a Complete Randomized Blok Design (CRBD) consisting of two factors with four replications. The first factor was PGRs and Boron formulation (without and with GA3 200 ppm+BAP 50 ppm+NAA 50 ppm+ IAA 300 ppm + 1500 ppm boron formulation). The second factor was variety (Bima Brebes, Maja Cipanas, Batu Ijo and Tajuk). Data were analyzed by F test with 95% confidence level followed by Duncan’s Multiple Range Test (DMRT) with 95% confidence level. The results showed that the application of PGRs formulations GA3 200 ppm + BAP 50 ppm + NAA 50 ppm +IAA 300 ppm + 1500 ppm boron on shallots of Tajuk varieties was able to increase number of leaves by 5 leaves and able to increase number of bulbs per clump of 3 bulbs compared to without the aplication of PGRs and boron formulations. The Tajuk variety provides the best growth in fresh weight of 16 g higher stalks and in the yield of fresh weight of bulbs per clump 23 g compared to the Batu Ijo variety. Shallots treated with 200 ppm GA3 + BAP 50 ppm + NAA 50 ppm +IAA 300 ppm + 1500 ppm boron have not shown an increase in growth and yield accros various shallot varieties.
Effect of NPK Fertilizer and Foliar Fertilizer on Chili Growth and Yield Anatalia, Rahma; Harsono, Puji; Yunindanova, Mercy Bientri; Purnomo, Djoko
Agrotechnology Research Journal Vol 6, No 2 (2022): Agrotechnology Research Journal
Publisher : Perkumpulan Agroteknologi/Agroekoteknologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (313.858 KB) | DOI: 10.20961/agrotechresj.v6i2.54540

Abstract

Chili consumption in Indonesia has increased. Efforts to increase chili production need fertilization through roots and leaf fertilization. The research aimed to obtain optimum intervals of NPK (nitrogen, phosphor, potassium) fertilization and kinds of foliar fertilizer for the growth and yield of chili. The research was conducted from April to August 2020 in Gayam Village, Sukoharjo Regency, Central Java, Indonesia. This study used an experiential method with a Randomized Complete Block Design (RCBD) of 2 factors. The first factor was an interval of NPK fertilization (16-16-16) with a concentration of 25 g.L-1 with a dose of 240 mL.plant-1. The interval of NPK fertilization consists of 4 levels, NPK fertilizer which was applied every 5, 10, 15, and 21 days. The second factor was foliar fertilizer which consists of A foliar fertilizer, B foliar fertilizer, and C foliar fertilizer. The results showed that the interval of NPK fertilizer affected the number of dichotomous branches, dry stove weight, cumulative fruit, cumulative fruit weight, and fruit length at 1st harvest. NPK fertilization every 15 days increased the dichotomous branches by 23.16%, dry stove weight by 37.98%, cumulative fruits by 37.41%, and cumulative fruit weight by 41.46%. B foliar fertilizer and A foliar fertilizer gave the same performance in dichotomous branches. There was no interaction effect between the interval of NPK fertilization and the kind of foliar fertilizer on all growth and yield variables. NPK fertilization optimum intervals as supplementary fertilizers should be given every 15 days. This research can be used as a reference for farmers in fertilizing chilies.
Role of Growth Regulators and Plant Media on Growth and Yields of Mint Sulandjari, Sulandjari; Pujiasmanto, Bambang; Supriyono, Supriyono; Harsono, Puji; Fajrin, Nurin Adyanisa
Agrotechnology Research Journal Vol 8, No 1 (2024): Agrotechnology Research Jurnal
Publisher : Perkumpulan Agroteknologi/Agroekoteknologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agrotechresj.v8i1.83639

Abstract

There is a significant industrial demand for products produced by the mint plant (Mentha piperita L.), yet Indonesia cannot meet all of that need. One of the efforts to increase mint plant production is using manure and atonic growth regulators in the right concentration. The method used was a Factorial Randomized Complete Block Design (RCBD) with two factors. The first factor was the composition of the planting media (soil: husk charcoal, soil: husk charcoal; cow manure, soil: husk charcoal: goat manure, soil: husk charcoal: chicken manure, with a ratio of 1: each: 1:1). The second factor was the atonic concentration (0, 1, 2, 3 mL.L-1). The results showed that using chicken manure gave the best results for growth and yield. Atonic administration with a concentration of 2.05 mL.L-1 gave optimum results on the fresh weight of roots, and a concentration of 2,06 mL.L-1 gave optimum results on the dry weight of roots. The combination of the two treatments did not significantly affect the observed variables.
Komposisi Media Tanam Organik terhadap Pertumbuhan dan Produksi Jahe Merah (Zingiber officinale var. rubrum) di Polybag Pujiasmanto, Bambang; Triharyanto, Eddy; Sulandjari, Sulandjari; Harsono, Puji; Pardono, Pardono; Widijanto, Herry; Nadhifatul Ardhina, Safira Nadhifatul; Setyaningrum, Desy
Agrotechnology Research Journal Vol 6, No 2 (2022): Agrotechnology Research Journal
Publisher : Perkumpulan Agroteknologi/Agroekoteknologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (205.653 KB) | DOI: 10.20961/agrotechresj.v6i2.51840

Abstract

The productivity of red ginger in Indonesia has decreased by 9,174 tons compared to 2017. The decrease is due to the very limited area of red ginger production, the method of red ginger cultivation, which is still very conventional, and the low amount of organic matter in the soil. This study examines the effect of planting media composition on the growth and production of red ginger. The study used a Completely Randomized Block Design (RCBD) with six treatments, namely control (soil without manure), cow manure: green manure: soil (1:1:1), P2 = goat manure: green manure: soil (1:1:1), green manure: soil (1:2), cow manure: soil (1:2), and goat manure: soil (1:2) and repeated four times. Composition media of goat manure: green fertilizer: soil with a ratio of 1:1:1 could increase the growth of red ginger in variable plant height, number of leaves in the clump, leaf area, and the weight of fresh stover with a yield of 52.7 cm; 10,75; 148,5; 2952.59 cm2; and 307.33 g. Composition of green fertilizer media: soil with a ratio of 1:2 could increase the weight of dry stover with a value of 39.32 g, and red ginger production includes fresh weight of rhizomes 111.32 g, storage weight of rhizomes 107.87 g, and rhizome volume 124.12 cm3.
Growth and Yield Responses of Red Chili to Various Foliar Fertilizers Harsono, Puji; Khakim, Moch. Irfan; Handoyo, Gani Cahyo; Supriyono, Supriyono
Agrotechnology Research Journal Vol 9, No 1 (2025): Agrotechnology Research Journal
Publisher : Perkumpulan Agroteknologi/Agroekoteknologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agrotechresj.v9i1.96737

Abstract

Red chili production in certain areas of Central Java, including Sendangijo Village, Wonogiri Regency, remains relatively low. One alternative to improve its growth and yield is foliar fertilization. This study aimed to determine the most effective type of foliar fertilizer for enhancing the growth and yield of red chili. The study was conducted from May to September 2023 in Sendangijo Village using a Randomized Complete Block Design with a single factor consisting of four treatments: no foliar fertilizer, foliar fertilizer A. NPK 11-8-6, B. NPK 20-15-15, and C. NPK 10-2-7, each with six replications. Observed variables included plant height, number of dichotomous branches, number of leaves, leaf area, fresh and dry weight of biomass, number of fruits, fresh weight of fruits per plant, and productivity per hectare. The result showed that the leaf area of red chili pepper given foliar fertilizer NPK 20-15-15  was larger than the foliar fertilizer NPK 11-8-6, NPK 10-2-7, and without foliar fertilizer. Foliar fertilizer NPK 20-15-15 also gave a heavier dry weight of biomass compared to foliar fertilizer NPK 11-8-6, NPK 10-2-7, and without foliar fertilizer. However, foliar fertilizer application did not significantly affect the number of fruits, the fresh weight of fruits per plant, or productivity per hectare.
Pengembangan Model Kemitraan Closed Loop Beras Organik Melalui Fasilitasi Petani Sanggar Rojolele di Desa Delanggu Harsono, Puji; Padmaningrum, Dwiningtyas; Supriyono, Supriyono
PRIMA: Journal of Community Empowering and Services Vol 9, No 1 (2025): June
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/prima.v9i1.80384

Abstract

Developing a Closed Loop Partnership Model for Organic Rice through Farmer Facilitation at Rojolele Studio in Delanggu Village. Rojolele rice development often faces problems in quality and operations. The closed-loop partnership model is one solution through the integration of the supply and value chain ecosystem from upstream to downstream, which mutually benefits all parties, including farmer groups. The Community Partnership Program by the Sebelas Maret University Service Team was carried out to facilitate increasing the competency and partnership of Sanggar Rojolele Delanggu farmers by implementing good agricultural practices (GAP) for organic rice products standardized by the Organic Certification Institute in Indonesia. Purposes of service: (1) increasing the hard skills & soft skills of farmers in developing organic Rojolele rice, (2) increasing productivity and farmer income, as long as (3) guaranteeing market certainty with competitive prices. The implementation method is through: (1) extension and training of farmers in the form of experiential learning through demonstration plots of organic Rojolele rice, (2) mentoring of low input Rojolele rice cultivation based on organic farming, and (3) facilitation of marketing networks for organic Rojolele rice harvest products with off taker PT. WSS Indonesia refers to quality standards according to SNI. The results of extension, training, and facilitation for farmers show an increase in farmers' knowledge and skills in implementing GAP for organic rice products and an increase in market absorption of products at higher prices.
Pemanfaatan Minyak Serai Sebagai Bahan Aktif Nanovirusida untuk Pengendalian Penyakit Kuning pada Cabai Septariani, Dwiwiyati Nurul; Hadiwiyono, Hadiwiyono; Harsono, Puji; Mawar, Munti'ah
PRIMA: Journal of Community Empowering and Services Vol 4, No 2 (2020): December
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/prima.v4i2.39797

Abstract

Cabai merupakan komoditas yang penting secara ekonomi bagi Kelompok Tani Sidoluhur dan Kelompok Wanita Tani Puteri Petani Mandiri di Desa Cabeyan, Kecamatan Bendosari, Kabupaten Sukoharjo. Minimnya pengetahuan petani tentang pengendalian OPT menyebabkan pertanaman cabai terkendala berbagai penyakit terutama virus kuning yang dapat menurunkan hasil hingga 100%. Akhir-akhir ini berkembang formulasi nanoteknologi yang dapat meningkatkan efektivitas pestisida nabati. Nanoteknologi pestisida adalah formulasi partikel nanopestisida yang meningkatkan kelarutan, tingkat disolusi, dan dispersi saat aplikasi sehingga meningkatkan efikasi pestisida. Salah satu jenisnya yaitu nanovirusida yang berfungsi menekan infeksi virus pada tanaman. Kegiatan pengabdian ini bertujuan memperkenalkan nanovirusida nabati untuk mengatasi masalah penyakit pada pertanaman cabai. Metode kegiatan berupa penyuluhan mengenai cara mengendalikan berbagai organisme pengganggu tanaman (OPT) pada cabai, pembuatan demplot pembibitan, serta pelatihan teknis budidaya cabai dengan nanovirusida nabati. Aplikasi nanovirusida di lahan cabai lokasi mitra terbukti efektif menurunkan intensitas penyakit kuning lebih dari 50% dibandingkan tanpa pengendalian. Hasil kegiatan yang diperoleh berupa peningkatan keterampilan petani pada pengendalian virus kuning dan serangga penularnya dengan nanovirusida.
Growth Response and Yields of Variety of Sorghum on Multiple NPK Fertilization in Dry Land Samanhudi, Samanhudi; Harsono, Puji; Hartati, Sri; Iswahyudi, Iswahyudi; Wicaksono, Naufal Ghazy
Journal of Biodiversity and Biotechnology Vol 4, No 1 (2024)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v4i1.108195

Abstract

Land degradation is a major constraint to agricultural productivity, with drylands particularly vulnerable to critical conditions. Sorghum (Sorghum bicolor L. Moench) is a promising crop for such environments due to its adaptability and dual-purpose value for both biomass and sugar-rich stalks. This study evaluated the growth and yield response of four sorghum varieties—Super, Suri, Numbu, and Keller—to different rates of compound NPK fertilizer under dryland conditions. The experiment was arranged in a Randomized Complete Block Design (RCBD) with two factors: sorghum varieties and five fertilizer doses (0, 10, 12, 14, and 16 g plant⁻¹), replicated three times. Data were subjected to ANOVA, and treatment means were compared using DMRT at the 5% significance level. Results showed that the Keller variety consistently produced the best agronomic performance, including plant height, number of leaves, stem segments, stem diameter, and biomass dry weight. Moreover, Keller combined with 12 g plant⁻¹ of NPK fertilizer produced the highest sap content (14.0% Brix), highlighting its potential as a dual-purpose sorghum. Leaf traits were most responsive at 14 g plant⁻¹, indicating that moderate-to-high fertilization enhances vegetative growth without excessive input. These findings demonstrate that varietal selection coupled with appropriate nutrient management is crucial for optimizing sorghum production in dryland systems. The study provides practical guidance for farmers and can inform future breeding and fertilizer recommendation programs for sustainable sorghum cultivation.
Co-Authors . Hadiwiyono Anatalia, Rahma Bambang Pujiasmanto Djoko Purnomo Dwiningtyas Padmaningrum, Dwiningtyas Dwiwiyati Nurul Septariani, Dwiwiyati Nurul Eddy Tri Haryanto, Eddy Tri Edi Purwanto Fajrin, Nurin Adyanisa Handoyo, Gani Cahyo HASANAH, NUR AZIZAH USWATUN ISWAHYUDI ISWAHYUDI Khakim, Moch. Irfan Mawar, Munti'ah Mercy Bientri Yunindanova Nadhifatul Ardhina, Safira Nadhifatul Pardono Pardono Putri, Ade Widya Samanhudi Samanhudi Setyaningrum, Desy Sri Hartati Sulandjari Sulandjari, Sulandjari Supriyono Supriyono Triharyanto, Eddy Wicaksono, Naufal Ghazy Widijanto, Herry