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All Journal JURNAL ILMU PERTANIAN JURNAL PEMULIAAN TANAMAN HUTAN AGRIVITA, Journal of Agricultural Science Kultivasi Agrosains: Jurnal Penelitian Agronomi Asian Journal of Agriculture Agrotechnology Research Journal SEMAR Tropical Plantation Journal Journal of Biodiversity and Biotechnology Indonesian Journal of Jamu

Samanhudi Samanhudi

Department Of Agrotechnology, Faculty Of Agriculture, Universitas Sebelas Maret

Author-ID : 369550

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Education Environmental Science Health Professions Languange, Linguistic, Communication & Media Medicine & Pharmacology Social Sciences

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Microclimate modification through shading and watering frequency treatments as efforts for ex situ conservation of pule pandak (Rauvolfia serpentina) SAMANHUDI, SAMANHUDI; PURWANTO, EDI; SULANDJARI, SULANDJARI; SETIYANINGSIH, ARY
Asian Journal of Agriculture Vol 1 No 01 (2017)
Publisher : Society for Indonesian Biodiversity & Universitas Mulawarman, Samarinda, Indonesia

Samanhudi, Purwanto E, Sulandjari, Setiyaningsih A. 2017. Microclimate modification through shading and watering frequency treatments as efforts for ex situ conservation of pule pandak (Rauvolfia serpentina). Asian J Agric 1: 35-39. Pule pandak (Rauvolfia serpentina Benth) is one of the rare medicinal plants. Exploration of nature continues to be done to meet the needs of the drug. That is why proper cultivation technique for pule pandak is required, by giving appropriate shading level and watering frequency to the growth and yield of pule pandak. The research aimed to examine the effect of shading and watering frequency on the growth and yield of pule pandak. The study was conducted from February to July 2009 in BBP Mondromino, Pokoh Village, Wonogiri at 141 meters above sea level. The experimental Design used in this research was a Split Plot Design consisted two factors and three repetitions. The first factor (main plot) was shading level consisted of three levels: 55%, 65%, and 75%. The second factor (sub plot) was watering frequency consisted of three levels: once three days, once five days and once seven days. Data were subjected to analysis of variance, followed by post hoc test of Duncan Multiple Range Test at 5% level. The reserpine content was analyzed descriptively. The results showed that no interaction between shading level and watering frequency on all variables. The shading level caused no significant effect on growth and yield of pule pandak except the leaf area. Watering frequency caused no significant effect on growth and yield of pule pandak except the chlorophyll content. The results showed that plants treated with watering frequency of five-day interval produced the highest chlorophyll content. Plants treated with shading level of 65% and watering frequency once five days produced the highest reserpine content.

Pertumbuhan In Vitro Tribulus terrestris dengan Perlakuan Indole Butyric Acid (IBA) dan Benzyl Amino Purine (BAP). Samanhudi Samanhudi; Bambang Pujiasmanto; Ahmad Yunus; Nurkholis Majid
AGRIUM: Jurnal Ilmu Pertanian Vol 24, No 1 (2021)
Publisher : UNIVERSITAS MUHAMMADIYAH SUMATERA UTARA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30596/agrium.v23i2.6916

Tribulus terrestris merupakan salah satu tanaman obat yang mempunyai beragam manfaat antara lain sebagai diuretik (peluruh kencing), meningkatkan kadar hormon testoteron, memulihkan vitalitas dan menambah kebugaran. Hambatan dari budidaya tanaman ini secara konvensional adalah relatif rendahnya persentase daya kecambah, bentuk bijinya yang kecil dan budidaya yang tergantung pada musim tertentu. Perbanyakan tanaman dengan menggunakan biji secara in vitro merupakan solusi yang dapat digunakan untuk memperbanyak tanaman ini. Penelitian bertujuan untuk mengetahui pertumbuhan in vitro T. terrestris dengan perlakuan IBA dan BAP. Penelitian menggunakan Rancangan Acak Lengkap (RAL) yang disusun secara faktorial. Faktor pertama adalah konsentrasi IBA dan faktor kedua adalah konsentrasi BAP. Analisis data dilakukan dengan uji DMRT dan deskriptif. Hasil penelitian menunjukkan adanya interaksi pemberian IBA 1 ppm + BAP 1 ppm menghasilkan tinggi tunas tertinggi dan jumlah akar terbanyak, interaksi pemberian IBA 1 ppm + BAP 1 ppm dan IBA 1,5 ppm + BAP 2 ppm menginduksi saat muncul akar tercepat. Pemberian IBA secara tunggal tidak mempengaruhi pertumbuhan eksplan T. terrestris, pemberian IBA 0,5 ppm dikombinasikan dengan BAP 2 ppm dapat menginduksi panjang akar terpanjang. Pemberian BAP 1 ppm dapat menginduksi saat muncul tunas tercepat, saat muncul daun tercepat dan jumlah daun terbanyak.

MULTIPLIKASI Aquilaria malaccensis DENGAN NAA DAN RAGI PADA KULTUR IN VITRO Samanhudi Samanhudi; Amalia Tetrani Sakya; Edi Purwanto; Indah Tri Retnosari
Jurnal Pemuliaan Tanaman Hutan Vol 15, No 1 (2021): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2021.15.1.51-59

Multiplication of Aquilaria malaccensis with naa and yeast growth regulators on in vitro culture. This study aims to obtain the best concentration of NAA and yeast extract for multiplication of agarwood on in vitro culture. This research was conducted from January to October 2020 at the Laboratory of Plant Physiology and Biotechnology, Faculty of Agriculture, Universitas Sebelas Maret, Surakarta. The experimental design used was a factorial completely randomized design (CRD) with two factors, namely NAA (0 ppm; 0.1 ppm; 0.2 ppm; and 0.3 ppm) and yeast extract (0 mg/l, 700 mg/l, 800 mg/l, and 900 mg/l).(+) The results showed that the combination of 0 ppm NAA and 900 mg/l yeast increased the number of shoots of A. malaccensis explants with the highest average number of 3.67 shoots. A single NAA concentration of 0 ppm was able to increase the number of leaves of explants of A. malaccensis with the highest average leaf rate of 24.5 leaves. A single yeast concentration of 0 mg/l was able to increase the number of leaves of A. malaccensis explants with an average of 22 leaves.

Comparison between the electrical conductivity method and radicle emergence test as a rapid test of sorghum seed vigor Nugraheni, Nugraheni; Pujiasmanto, Bambang; Samanhudi, Samanhudi; Sakya, Amalia Tetrani
Kultivasi Vol 22, No 2 (2023): Jurnal Kultivasi
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kultivasi.v22i2.46547

Sorghum (Sorghum bicolor L. Moench) belongs to multipurpose crops. All parts of sorghum can be used both as main products and derivative products; some derivative products produced from the sorghum plant consist of sugar, bioethanol, biomass, handicraft raw materials and starch. This study aims to determine the time required for testing the vigor and viability of sorghum seeds using the electrical conductivity and radicle emergence methods. The study was conducted in two experimental stages using a completely randomized design. The first experiment consisted of two factors: the number of sorghum seeds (40, 70 and 100 grains) and the volume of soaking water (75, 100, 125, and 150 ml). The second experiment consisted of two factors: research method (germination/control method, electrical conduction method, and radicle emergence method) and varieties (consisted of Numbu, Kawali, Suri 3, and Suri 4). The first experiment's results showed that the best electrical conductivity method on Suri 4 varieties were 40 seeds and 150 ml water volume. The electrical conductivity value is negatively correlated with Germination capacity, vigor index, growth of speed, maximum growth potential, and sprout growth rate. Time needed for Electrical Conductivity method in this study was three days. The germination period of 96 hours gives the best results on the radicle emergence of sorghum seed varieties. Vigor index, growth of speed, germination capacity, and maximum growth potential are positively correlated with the value of radicula emergence.Keywords: Electrical Conductivity, Radicle emergence, Vigor, Viability, Germination

Studies of Shading Levels and Nutrition Sources on Growth, Yield and Andrographolide Content of Sambiloto (Andrographis paniculata Ness) Purwanto, Edi; Samanhudi, Samanhudi; Sudarmi, Sudarmi
AGRIVITA Journal of Agricultural Science Vol 33, No 3 (2011)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v33i3.73

Growth and biochemical content of medicinal crops are influenced by agroecosystems characteristics . The objective of this research was to determine the optimum shading level and type of fertilizer as sources of nutrition on the growth, yield, and andrographolide content of sambiloto. The experiment used Split Plot Design with basic design of Randomized Complete Block Design arranged with two treatment factors, with three replications. The first factor as the main plot was shading levels, namely without shading, 25% shading, 50% shading, and 75% shading. The second factor as the sub plot was sources of nutrition reprented by type of fertilizer, namely NPK fertilizer, cow stable fertilizer, and compost fertilizer. The result of research indicated that shading level and the kind of nutrition influenced some growth and yield variables such as number of leaves, number of branches, plant height, plant dry weight and simplisia weight, and andrographolide content. Interaction of shading level at 25% and straw compost fertilizer performed best in growth characteristics, while the highest andrographolide content resulted from the treatment combination of 50% shading level and straw compost fertilizer.Keywords: fertilizer,nutrition, shading, andrographolide, Andrographis paniculata

In Vitro Propagation of Temulawak (Curcuma xanthorrhiza Roxb.) Samanhudi, Samanhudi; Yunus, Ahmad; Pujiasmanto, Bambang; Saras, Anindya
Jurnal Jamu Indonesia Vol. 2 No. 2 (2017): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v2i2.30

Problems encountered in the development of herbal medicine industry is that most of the raw materials (80%) came from the forest or natural habitats and the rest (20%) of the results of traditional cultivation. The research is focused to get the propagation techniques of temulawak by in vitro using various combinations of IBA and BAP concentration, each consisting of 0, 1, 2, 3, 4 ppm. The purpose of this research is to gain a plant propagation technique of temulawak through the use of IBA and BAP concentration so as to provide a quality seeds. The results showed that the highest shoots obtained at 1 ppm IBA and 3 ppm BAP treatment with a buds height of 15.9 cm. Treatment of 0 ppm IBA and 4 ppm BAP produces the highest buds number by 4 buds. While the highest number of leaves obtained in 2 ppm IBA and 3 ppm BAP treatment with total of 18 leafs. The highest length of leaf obtained in the treatment of 3 ppm IBA and 2 ppm BAP with a length of 14.6 cm. Variable highest number of roots was obtained at treatment of 4 ppm IBA and 1 ppm BAP with root number exceeds 30 pieces with very many hair roots and the highest root length was obtained at treatment of 2 ppm IBA and 0 ppm BAP with a length of 15.6 cm. Based on these results it can be concluded that the IBA and BAP treatment at various concentrations can affect the propagation and growth of temulawak explants.

Rice response to cadmium bioremediation using sorghum and mycorrhiza HARSONO, PUJI; HASANAH, NUR AZIZAH USWATUN; PURWANTO, EDI; SAMANHUDI, SAMANHUDI
Asian Journal of Agriculture Vol. 9 No. 2 (2025)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/asianjagric/g090219

Abstract. Harsono P, Hasanah NAU, Purwanto E, Samanhudi. 2025. Rice response to cadmium bioremediation using sorghum and mycorrhiza. Asian J Agric 9: 522-532. Cadmium (Cd) contamination at 2.9 mg kg-¹ in paddy soils reduces rice productivity and threatens food safety. This study aimed to evaluate the effectiveness of sorghum (Sorghum bicolor) phytoremediation combined with Arbuscular Mycorrhizal Fungi (AMF) in improving rice growth under mild Cd contamination. A factorial randomized complete block design with two cropping systems (seed vs. ratoon, where the same crop is re-grown from the stubble or roots of the first crop) and seven remediation treatments (control; three sorghum varieties (Super 1, Samurai 1, Kawali) combine with/without 10 g AMF plot-¹) was applied in 42 plots (3×4 m). Rice cv. IR 64 was transplanted 30 days after remediation. Significant interactions (p<0.05) between cropping system and remediation treatment occurred for plant height and leaf area. The Kawali + AMF treatment produced the most significant gains relative to the control: plant height +7.7%, leaf area +207%, chlorophyll +236% and total dry biomass +152%, while extending the vegetative phase by 23%. Improvements were consistent across sorghum seed and sorghum ratoon systems, indicating that remediation benefits persist beyond the first harvest. Ratoon sorghum-maintained remediation benefits, with 7-10% taller plants and 8% greater leaf area than the control, despite slightly reduced canopy size compared with seed sorghum; all sorghum treatments delayed flowering by ~11 days, indicating reduced Cd stress. High-biomass sorghum, especially Kawali, combined with AMF, enhances rice growth and physiology while stabilizing Cd, providing a scalable remediation strategy for sustainable rice production in Cd-contaminated tropical agroecosystems.

Effects of nutrient solution and substrate on Limnocharis flava performance in hydroponic systems SESANTI, RIZKA NOVI; PURWANTO, EDI; SAMANHUDI, SAMANHUDI; SUDADI, SUDADI
Asian Journal of Agriculture Vol. 9 No. 2 (2025)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/asianjagric/g090225

Abstract. Sesanti RN, Purwanto E, Samanhudi, Sudadi. 2025. Effects of nutrient solution and substrate on Limnocharis flava performance in hydroponic systems. Asian J Agric 9: 590-597. Limnocharis flava is a promising aquatic vegetable for substrate-based hydroponic cultivation. However, the optimal combination of nutrient concentrations and substrate types to maximize growth under hydroponic conditions remains unclear. This study evaluated five nutrient solution concentrations (0, 0.8, 1.6, 2.4, and 3.2 dS m-¹) and three substrate types (volcanic sand, rice husk charcoal, and a 1:1 mixture) in a Randomized Complete Block Design (RCBD) with three replications. Nutrient concentrations of 0.8 and 1.6 dS m-¹ produced the highest plant height (28.48 cm and 28.20 cm), number of leaves (11.31 and 11.03), shoot dry weight (4.61 g and 4.74 g), root fresh weight (13.20 g and 12.43 g), and root dry weight (0.76 g and 0.74 g). Both lower and higher concentrations inhibited growth, indicating optimal thresholds for nutrient uptake. Chlorophyll a, b, and total chlorophyll contents were similar across the 0.8-3.2 dS m-¹ range, but consistently higher than the control. The leaf greenness index showed a significant interaction between nutrient concentration and substrate type, with the highest value at 2.4 dS m-¹ in the mixed substrate. A nutrient concentration of 1.6 dS m-¹ produced the best overall performance. Based on these results, a combination of 1.6 dS m-¹ nutrient solution and a 1:1 mixture of volcanic sand and rice husk charcoal is recommended for the cultivation of L. flava. These findings establish baseline guidelines for hydroponic cultivation of L. flava and highlight its potential to advance precision hydroponics for indigenous vegetables, thereby contributing to food security and sustainable agriculture.

Effect of Dolomite and NPK on The Growth and Yield of off-Season Shallots Iswahyudi, Iswahyudi; Haryanto, Eddy Tri; Supriyono, Supriyono; Al-batul, Fitrotunnisa; Samanhudi, Samanhudi; Irawati, Erni; Rahayu, Muji; Purwanto, Edi
Tropical Plantation Journal Vol 5, No 1 (2026): TROPICAL PLANTATION JOURNAL
Publisher : Akademi Komunitas Perkebunan Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56125/tpj.v5i1.74

Shallot is a high-value horticultural commodity. Its productivity often declines due to suboptimal soil conditions, such as low pH and imbalanced nutrient availability, especially when cultivated off-season. This study aimed to determine the optimal combination of dolomite and NPK (Nitrogen, Phosphorus, Potassium) fertilizer for the growth and yield of shallot cultivated in the off-season. The research was conducted in Ngringo Village, Jaten District, Karanganyar Regency from February-April 2025. The method used was a factorial Completely Randomized Design (CRD) with two factors and three replications. The first factor was dolomite doses (0; 2000; and 4000 kg.ha-1) and NPK fertilizer doses (250; 375; and 500 kg.ha-1). Observed parameters were plant height, number of leaves, fresh and dry biomass weight, fresh and dry bulb weight, number of bulbs per plant, and bulb diameter. Data were analyzed by analysis of variance (ANOVA) at a 95% significance level and continued with Duncan’s Multiple Range Test (DMRT) with 95% significance level when significant differences were found. The results showed no significant interaction between dolomite and NPK fertilizer on shallot growth and yield. Dolomite at 2000 kg.ha-1 significantly increased the number of leaves, while NPK fertilizer had no significant effect. However, 250 kg.ha-1 of NPK fertilizer produced similar results to higher doses, indicating potential fertilizer efficiency. Environmental conditions such as high humidity and low light intensity likely influenced treatment effectiveness.

Effect of Water Availability on the Growth and Secondary Metabolites of Zodia (Evodia suaveolens) Setyawati, Andriyana; Purwanto, Edi; Yunus, Ahmad; Samanhudi, Samanhudi; Sakya, Amalia Tetrani; Rahayu, Muji; Purnomo, Djoko; Arniputri, Retna Bandriyati; Cahyo, Gani; Dwiyanti, Qonita Rahma; Egra, Saat; Talitha, Okky; Muthi’ah, Alfida
Agrosains : Jurnal Penelitian Agronomi Vol 25, No 2 (2023): Agrosains: Jurnal Penelitian Agronomi
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agsjpa.v25i2.81528

Zodia are included in medicinal plants because it contains secondary metabolites such as flavonoids, tannins, alkaloids, steroids/triterpenoids that have many benefit and also as mosquito repellents. The growth and content of secondary metabolites in plants can be affected by environmental factors such as water availability. Research on the cultivation of this crop is still limited. Environmental modification in cultivation is needed to increase its secondary metabolites, and the study of the potential of this plant as medicine are necessary. This study aimed to determine the effect of water availability on the growth and secondary metabolites of zodia. The research was carried out in July-November 2021 in Greenhouse, Faculty of Agriculture, Universitas Sebelas Maret, Surakarta. The method used was a completely randomized design, one treatment factor with 4 levels namely, 100, 80, 60 and 40% of field capacity. Each treatment was repeated 6 times so that there were 24 experimental polybags. The data analysis used is Analysis of Variance, and the Duncan’s Multiple Range Test at the 5% level. The results showed that the availability of water at a level of 60% field capacity gave the best average growth parameters (plant height and number of leaves) among other treatments. The flavonoid content in each treatment showed the same average. The results of the GCMS (Gas Cromatography and Mass Spectroscopy) test of zodia leaves showed that there were dominant bioactive compounds, namely ethylbenzene, octadecamethyl cyclononasiloxane, pentadecanoid acid and heptadecanoid acid. It is suspected that the content of these compounds has the potential as antifungal, antibacterial, antioxidant and anticancer.

Co-Authors Adani, Muhammad Naufal Adi Ratriyanto Afanin, Hasna Agastya Putra Pradipta Agnofi Merdeka Efendi Ahmad Yunus Ahmad Yunus Ahmad Yunus Ahmad Yunus Al-batul, Fitrotunnisa Amalia Tetrani Sakya Amalia Tetrani Sakya Amalia Tetrani Sakya, Amalia Tetrani Anif Nur Artanti, Anif Nur ARI SUSILOWATI Arniputri, Retna Bandriyati Bachtiar, Zuraida Anniswa Bambang Pujiasmanto Bambang Pujiasmanto Bambang Pujiasmanto Bambang Pujiasmanto Cahyadi, Muhammad Aji Cahyo, Gani Dewi, Reni Surya Dhimas Taufika Putra Djoko Purnomo Dwi Hardjoko Dwiyanti, Qonita Rahma Eddy Tri Haryanto, Eddy Tri Edi Purwanto Edi Purwanto Edi Purwanto Edi Purwanto Egra, Saat Endang Setyowati Endang Suprapti Endang Yuniastuti Erni Irawati Eviani, Eviani Fitri Aprillia Fitria Roviqowati Hadiandra Harfiansyah Handoyo, Gani Cahyo Hardjoko, Dwi HASANAH, NUR AZIZAH USWATUN Himawan Joko Riswanda Ida Rumia Manurung Indah Tri Retnosari Indrawan Fahmi ISWAHYUDI ISWAHYUDI Iwan Dewangga Mochammad Danny Sukardan, Mochammad Danny Muhammad Imam Wicaksono Muhammad Imam Wicaksono Muji Rahayu Muji Rahayu Muthi’ah, Alfida Nandariyah, Nandariyah Nugraheni Nugraheni Nurkholis Majid Paramasari Dirgahayu Prihanto, Joko Puji Harsono, Puji Purwanto Purwanto Rahma, Nadia Alifia Rahmanto Rahmanto Riandy Adhitya Rizka Novi Sesanti S.Pd. M Kes I Ketut Sudiana . Saputri, Dewi Selvia Maulida Saras, Anindya SETIYANINGSIH, ARY Setyawan, Kevin Putra Setyawati, Andriyana Sigit PRASTOWO Siti Mardhika Sari Sri Hartati Sri Mulyani Sudadi Sudadi Sudarmi Sudarmi Sukmawati, Anne Sulandjari Sulandjari, Sulandjari Supriyono Supriyono Talitha, Okky Venty Suryanti Wartoyo Wartoyo Wicaksono, Naufal Ghazy Yunus, Ahmad

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