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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmu Pertanian JURNAL ILMU PERTANIAN JURNAL PEMULIAAN TANAMAN HUTAN AGRIVITA, Journal of Agricultural Science Kultivasi AGRISAINTIFIKA Jurnal Ilmu-ilmu Pertanian Agrosains: Jurnal Penelitian Agronomi jurnal Ilmu Pertanian (Agricultural Science) Jurnal Jamu Indonesia Jurnal Agrotek Indonesia Asian Journal of Agriculture Agrotechnology Research Journal SEMAR Tropical Plantation Journal Journal of Biodiversity and Biotechnology Gunung Djati Conference Series Indonesian Journal of Jamu Biodiversitas Journal of Biological Diversity
Samanhudi
Departement Of Agrotechnology, Faculty Of Agriculture, Universitas Sebelas Maret
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Social Sciences Other

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Microclimate modification through shading and watering frequency treatments as efforts for ex situ conservation of pule pandak (Rauvolfia serpentina) SAMANHUDI, SAMANHUDI; PURWANTO, EDI; SULANDJARI, SULANDJARI; SETIYANINGSIH, ARY
Asian Journal of Agriculture Vol 1 No 01 (2017)
Publisher : Society for Indonesian Biodiversity & Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (216.983 KB) | DOI: 10.13057/asianjagric/g010107

Abstract

Samanhudi, Purwanto E, Sulandjari, Setiyaningsih A. 2017. Microclimate modification through shading and watering frequency treatments as efforts for ex situ conservation of pule pandak (Rauvolfia serpentina). Asian J Agric 1: 35-39. Pule pandak (Rauvolfia serpentina Benth) is one of the rare medicinal plants. Exploration of nature continues to be done to meet the needs of the drug. That is why proper cultivation technique for pule pandak is required, by giving appropriate shading level and watering frequency to the growth and yield of pule pandak. The research aimed to examine the effect of shading and watering frequency on the growth and yield of pule pandak. The study was conducted from February to July 2009 in BBP Mondromino, Pokoh Village, Wonogiri at 141 meters above sea level. The experimental Design used in this research was a Split Plot Design consisted two factors and three repetitions. The first factor (main plot) was shading level consisted of three levels: 55%, 65%, and 75%. The second factor (sub plot) was watering frequency consisted of three levels: once three days, once five days and once seven days. Data were subjected to analysis of variance, followed by post hoc test of Duncan Multiple Range Test at 5% level. The reserpine content was analyzed descriptively. The results showed that no interaction between shading level and watering frequency on all variables. The shading level caused no significant effect on growth and yield of pule pandak except the leaf area. Watering frequency caused no significant effect on growth and yield of pule pandak except the chlorophyll content. The results showed that plants treated with watering frequency of five-day interval produced the highest chlorophyll content. Plants treated with shading level of 65% and watering frequency once five days produced the highest reserpine content.
Pertumbuhan In Vitro Tribulus terrestris dengan Perlakuan Indole Butyric Acid (IBA) dan Benzyl Amino Purine (BAP). Samanhudi Samanhudi; Bambang Pujiasmanto; Ahmad Yunus; Nurkholis Majid
AGRIUM: Jurnal Ilmu Pertanian Vol 24, No 1 (2021)
Publisher : UNIVERSITAS MUHAMMADIYAH SUMATERA UTARA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30596/agrium.v23i2.6916

Abstract

Tribulus terrestris merupakan salah satu tanaman obat yang mempunyai beragam manfaat antara lain sebagai diuretik (peluruh kencing), meningkatkan kadar hormon testoteron, memulihkan vitalitas dan menambah kebugaran. Hambatan dari budidaya tanaman ini secara konvensional adalah relatif rendahnya persentase daya kecambah, bentuk bijinya yang kecil dan budidaya yang tergantung pada musim tertentu. Perbanyakan tanaman dengan menggunakan biji secara in vitro merupakan solusi yang dapat digunakan untuk memperbanyak tanaman ini. Penelitian bertujuan untuk mengetahui pertumbuhan in vitro T. terrestris dengan perlakuan IBA dan BAP. Penelitian menggunakan Rancangan Acak Lengkap (RAL) yang disusun secara faktorial. Faktor pertama adalah konsentrasi IBA dan faktor kedua adalah konsentrasi BAP. Analisis data dilakukan dengan uji DMRT dan deskriptif. Hasil penelitian menunjukkan adanya interaksi pemberian IBA 1 ppm + BAP 1 ppm menghasilkan tinggi tunas tertinggi dan jumlah akar terbanyak, interaksi pemberian IBA 1 ppm + BAP 1 ppm dan IBA 1,5 ppm + BAP 2 ppm menginduksi saat muncul akar tercepat. Pemberian IBA secara tunggal tidak mempengaruhi pertumbuhan eksplan T. terrestris, pemberian IBA 0,5 ppm dikombinasikan dengan BAP 2 ppm dapat menginduksi panjang akar terpanjang. Pemberian BAP 1 ppm dapat menginduksi saat muncul tunas tercepat, saat muncul daun tercepat dan jumlah daun terbanyak.
MULTIPLIKASI Aquilaria malaccensis DENGAN NAA DAN RAGI PADA KULTUR IN VITRO Samanhudi Samanhudi; Amalia Tetrani Sakya; Edi Purwanto; Indah Tri Retnosari
Jurnal Pemuliaan Tanaman Hutan Vol 15, No 1 (2021): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2021.15.1.51-59

Abstract

Multiplication of Aquilaria malaccensis with naa and yeast growth regulators on in vitro culture. This study aims to obtain the best concentration of NAA and yeast extract for multiplication of agarwood on in vitro culture. This research was conducted from January to October 2020 at the Laboratory of Plant Physiology and Biotechnology, Faculty of Agriculture, Universitas Sebelas Maret, Surakarta. The experimental design used was a factorial completely randomized design (CRD) with two factors, namely NAA (0 ppm; 0.1 ppm; 0.2 ppm; and 0.3 ppm) and yeast extract (0 mg/l, 700 mg/l, 800 mg/l, and 900 mg/l).(+) The results showed that the combination of 0 ppm NAA and 900 mg/l yeast increased the number of shoots of A. malaccensis explants with the highest average number of 3.67 shoots. A single NAA concentration of 0 ppm was able to increase the number of leaves of explants of A. malaccensis with the highest average leaf rate of 24.5 leaves. A single yeast concentration of 0 mg/l was able to increase the number of leaves of A. malaccensis explants with an average of 22 leaves.
In Vitro Propagation of Temulawak (Curcuma xanthorrhiza Roxb.) Samanhudi Samanhudi; Ahmad Yunus; Bambang Pujiasmanto; Anindya Saras
Jurnal Jamu Indonesia Vol. 2 No. 2 (2017): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (857.622 KB) | DOI: 10.29244/jji.v2i2.30

Abstract

Problems encountered in the development of herbal medicine industry is that most of the raw materials (80%) came from the forest or natural habitats and the rest (20%) of the results of traditional cultivation. The research is focused to get the propagation techniques of temulawak by in vitro using various combinations of IBA and BAP concentration, each consisting of 0, 1, 2, 3, 4 ppm. The purpose of this research is to gain a plant propagation technique of temulawak through the use of IBA and BAP concentration so as to provide a quality seeds. The results showed that the highest shoots obtained at 1 ppm IBA and 3 ppm BAP treatment with a buds height of 15.9 cm. Treatment of 0 ppm IBA and 4 ppm BAP produces the highest buds number by 4 buds. While the highest number of leaves obtained in 2 ppm IBA and 3 ppm BAP treatment with total of 18 leafs. The highest length of leaf obtained in the treatment of 3 ppm IBA and 2 ppm BAP with a length of 14.6 cm. Variable highest number of roots was obtained at treatment of 4 ppm IBA and 1 ppm BAP with root number exceeds 30 pieces with very many hair roots and the highest root length was obtained at treatment of 2 ppm IBA and 0 ppm BAP with a length of 15.6 cm. Based on these results it can be concluded that the IBA and BAP treatment at various concentrations can affect the propagation and growth of temulawak explants.
Pengaruh Kascing dan Mikoriza terhadap Pertumbuhan dan Hasil Tribulus terrestris Samanhudi Samanhudi
Jurnal Agrotek Indonesia (Indonesian Journal of Agrotech) Vol 2 No 1 (2017): Jurnal Agrotek Indonesia (Indonesian Journal of Agrotech)
Publisher : Faculty of Agriculture University of Singaperbangsa Karawang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (54.752 KB) | DOI: 10.33661/jai.v2i1.711

Abstract

Tribulus terrestris is one of the medicinal plants consumed in Indonesia that contain a variety of components.Saponins in Tribulus is an active component that can eliminate sexual dysfunction, improve erection function, protectthe myocardium against ischemia/reperfusion injury and hypertension, and coronary heart disease. Various benefitscontained in the Tribulus resulted in the tremendous opportunity to develop Tribulus in Indonesia. The reality inIndonesia is still very few farmers who develop Tribulus as the mainstay in planting, so that the yield and productionof these plant are needed to meet market demand in Indonesia. It has prompted the need for proper cultivation and inaccordance with the use of vermicompost fertilizer and mycorrhiza. This research aims to determine the effect of adose of vermicompost and mycorrhiza on growth and yield of Tribulus. This research was conducted at theexperimental farm of Faculty of Agriculture, Universitas Sebelas Maret in the village of Sukosari, Jumantono,Karanganyar. The research was conducted for three months, in June until August 2016. The design used in thisresearch is a Completely Randomized Block Design (CRBD) arranged as factorial, consists of two factors oftreatment with three experimental blocks. The first treatment factors that dose of vermicompost fertilizer consists offour levels: 0, 10, 20, 30 tonha-1. The second treatment factor is the dose of mycorrhiza consists of three levels: 0, 50,100 sporesplant-1. The data were analyzed by F test or analysis of variants (Anova) continued with Duncan MultipleRange Test (DMRT) level of 5%. Mycorrhiza treatment dose of 100 sporesplant-1 gave the highest yield on the weightof the wet stover (844.63 g), time of the flowers appear (7 DAP), the number of branches (8 branchesplant-1), sum offruit (627 fruits), and seed number (3,135 seeds). Mycorrhiza treatment dose of 50 sporesplant-1 gives the highest yieldon plant height (167.8 cm). Meanwhile, vermicompost fertilizer treatment dose of 20 tonha-1 gives the best results onroot volume (38.31 cm3).Keywords: medicinal plant, mycorrhiza, Tribulus terrestris, vermicompost.
Application of NAA and BA to Calotropis gigantea (L.) W.T. Aiton in vitro Muji Rahayu; Tyas Alivia Wulandari; Amalia Tetrani Sakya; Andriyana Setyawati; Sri Hartati; Samanhudi Samanhudi
Jurnal Ilmu Pertanian Vol 8, No 2 (2023): August
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.83664

Abstract

Crown flower (Calotropis gigantea (L.)) is a weed plant potentially to be used as raw material for textile fibers yet the utilization is not optimal. Efforts to optimize the utilization of C. gigantea are needed. This research aimed to determine the appropriate concentration of plant growth regulators, including NAA (Naphthalene Acetic Acid) and BA (Benzyl Adenine) to stimulate optimal growth of C. gigantea to be used as the basis for in vitro propagation of C. gigantea. The research was arranged in a completely randomized design (CRD). Explants were planted on Murashige and Skoog (MS) medium with various concentrations of NAA (0; 0.5; 1; 1.5; and 2 ppm) and BA (0; 1; 2; 3; and 4 ppm). Based on the research result, the addition of 3 ppm BA followed by increasing the concentration of NAA to 1.5 ppm could increase the number of C. gigantea shoots, and giving 1 ppm BA followed by adding 1.5 to 2 ppm NAA increased shoot height. The best plantlet response was found in media with 0 ppm NAA and 4 ppm BA, which could produce an optimal shoot height of 5.9 cm and a large number of shoots of 5.67 shoots. Increasing NAA concentration retarded root formation and reduced the root length and number of leaves, while the medium without NAA gave the earliest root emergence of 11 days after planting, the number of leaves of 20 strands, and the longest root of 10.9 cm. Applying a single substance BA did not accelerate all the variables observed.
Application of NAA and BA to Calotropis gigantea (L.) W.T. Aiton in vitro Muji Rahayu; Tyas Alivia Wulandari; Amalia Tetrani Sakya; Andriyana Setyawati; Sri Hartati; Samanhudi Samanhudi
Jurnal Ilmu Pertanian Vol 8, No 2 (2023): August
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.83664

Abstract

Crown flower (Calotropis gigantea (L.)) is a weed plant potentially to be used as raw material for textile fibers yet the utilization is not optimal. Efforts to optimize the utilization of C. gigantea are needed. This research aimed to determine the appropriate concentration of plant growth regulators, including NAA (Naphthalene Acetic Acid) and BA (Benzyl Adenine) to stimulate optimal growth of C. gigantea to be used as the basis for in vitro propagation of C. gigantea. The research was arranged in a completely randomized design (CRD). Explants were planted on Murashige and Skoog (MS) medium with various concentrations of NAA (0; 0.5; 1; 1.5; and 2 ppm) and BA (0; 1; 2; 3; and 4 ppm). Based on the research result, the addition of 3 ppm BA followed by increasing the concentration of NAA to 1.5 ppm could increase the number of C. gigantea shoots, and giving 1 ppm BA followed by adding 1.5 to 2 ppm NAA increased shoot height. The best plantlet response was found in media with 0 ppm NAA and 4 ppm BA, which could produce an optimal shoot height of 5.9 cm and a large number of shoots of 5.67 shoots. Increasing NAA concentration retarded root formation and reduced the root length and number of leaves, while the medium without NAA gave the earliest root emergence of 11 days after planting, the number of leaves of 20 strands, and the longest root of 10.9 cm. Applying a single substance BA did not accelerate all the variables observed.
Comparison between the electrical conductivity method and radicle emergence test as a rapid test of sorghum seed vigor Nugraheni, Nugraheni; Pujiasmanto, Bambang; Samanhudi, Samanhudi; Sakya, Amalia Tetrani
Kultivasi Vol 22, No 2 (2023): Jurnal Kultivasi
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kultivasi.v22i2.46547

Abstract

Sorghum (Sorghum bicolor L. Moench) belongs to multipurpose crops. All parts of sorghum can be used both as main products and derivative products; some derivative products produced from the sorghum plant consist of sugar, bioethanol, biomass, handicraft raw materials and starch. This study aims to determine the time required for testing the vigor and viability of sorghum seeds using the electrical conductivity and radicle emergence methods. The study was conducted in two experimental stages using a completely randomized design. The first experiment consisted of two factors: the number of sorghum seeds (40, 70 and 100 grains) and the volume of soaking water (75, 100, 125, and 150 ml). The second experiment consisted of two factors: research method (germination/control method, electrical conduction method, and radicle emergence method) and varieties (consisted of Numbu, Kawali, Suri 3, and Suri 4). The first experiment's results showed that the best electrical conductivity method on Suri 4 varieties were 40 seeds and 150 ml water volume. The electrical conductivity value is negatively correlated with Germination capacity, vigor index, growth of speed, maximum growth potential, and sprout growth rate. Time needed for Electrical Conductivity method in this study was three days. The germination period of 96 hours gives the best results on the radicle emergence of sorghum seed varieties. Vigor index, growth of speed, germination capacity, and maximum growth potential are positively correlated with the value of radicula emergence.Keywords: Electrical Conductivity, Radicle emergence, Vigor, Viability, Germination
Studies of Shading Levels and Nutrition Sources on Growth, Yield and Andrographolide Content of Sambiloto (Andrographis paniculata Ness) Purwanto, Edi; Samanhudi, Samanhudi; Sudarmi, Sudarmi
AGRIVITA Journal of Agricultural Science Vol 33, No 3 (2011)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v33i3.73

Abstract

Growth and biochemical content of medicinal crops are influenced by agroecosystems characteristics . The objective of this research was to determine the optimum shading level and type of fertilizer as sources of nutrition on the growth, yield, and andrographolide content of sambiloto. The experiment used Split Plot Design with basic design of Randomized Complete Block Design arranged with two treatment factors, with three replications. The first factor as the main plot was shading levels, namely without shading, 25% shading, 50% shading, and 75% shading. The second factor as the sub plot was sources of nutrition reprented by type of fertilizer, namely NPK fertilizer, cow stable fertilizer, and compost fertilizer. The result of research indicated that shading level and the kind of nutrition influenced some growth and yield variables such as number of leaves, number of branches, plant height, plant dry weight and simplisia weight, and andrographolide content. Interaction of shading level at 25% and straw compost fertilizer performed best in growth characteristics, while the highest andrographolide content resulted from the treatment combination of 50% shading level and straw compost fertilizer.Keywords: fertilizer,nutrition, shading, andrographolide, Andrographis paniculata
In Vitro Propagation of Temulawak (Curcuma xanthorrhiza Roxb.) Samanhudi, Samanhudi; Yunus, Ahmad; Pujiasmanto, Bambang; Saras, Anindya
Jurnal Jamu Indonesia Vol. 2 No. 2 (2017): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v2i2.30

Abstract

Problems encountered in the development of herbal medicine industry is that most of the raw materials (80%) came from the forest or natural habitats and the rest (20%) of the results of traditional cultivation. The research is focused to get the propagation techniques of temulawak by in vitro using various combinations of IBA and BAP concentration, each consisting of 0, 1, 2, 3, 4 ppm. The purpose of this research is to gain a plant propagation technique of temulawak through the use of IBA and BAP concentration so as to provide a quality seeds. The results showed that the highest shoots obtained at 1 ppm IBA and 3 ppm BAP treatment with a buds height of 15.9 cm. Treatment of 0 ppm IBA and 4 ppm BAP produces the highest buds number by 4 buds. While the highest number of leaves obtained in 2 ppm IBA and 3 ppm BAP treatment with total of 18 leafs. The highest length of leaf obtained in the treatment of 3 ppm IBA and 2 ppm BAP with a length of 14.6 cm. Variable highest number of roots was obtained at treatment of 4 ppm IBA and 1 ppm BAP with root number exceeds 30 pieces with very many hair roots and the highest root length was obtained at treatment of 2 ppm IBA and 0 ppm BAP with a length of 15.6 cm. Based on these results it can be concluded that the IBA and BAP treatment at various concentrations can affect the propagation and growth of temulawak explants.
Co-Authors Adi Ratriyanto Agastya Putra Pradipta Agnofi Merdeka Efendi AHMAD YUNUS Ahmad Yunus Ahmad Yunus Alfida Muthi’ah AMALIA TETRANI SAKYA AMALIA TETRANI SAKYA Amalia Tetrani Sakya Amalia Tetrani Sakya Amalia Tetrani Sakya Amalia Tetrani Sakya Amalia Tetrani Sakya Amalia Tetrani Sakya Amalia Tetrani Sakya Amalia Tetrani Sakya, Amalia Tetrani ANDRIYANA SETYAWATI ANDRIYANA SETYAWATI Andriyana Setiyawati Andriyana Setyawati Anif Nur Artanti, Anif Nur Anindya Saras ANIVERSARI APRIANA Anne Sukmawati ARI SUSILOWATI ATMITRI SISHARMINI Ayu Lestari BAMBANG PUJIASMANTO Bambang Pujiasmanto Bambang Pujiasmanto Bambang Pujiasmanto Bambang Pujiasmanto Bambang Pujiasmanto Dewi Selvia Maulida Saputri Dewi, Reni Surya Dhimas Taufika Putra DIDIK SUDIHARDJO Djoko Purnomo Djoko Purnomo Djoko Purnomo Dwi Hardjoko Dwi Harjoko Eddy Tri Haryanto EDI PURWANTO EDI PURWANTO EDI PURWANTO Edi Purwanto Edi Purwanto EDI PURWANTO EDI PURWANTO Edi Purwanto Edi Purwanto Edi Purwanto Edi Purwanto Edi Purwanto Edi Purwanto EKA HANDAYANTA Elvy Carolina Pane ENDANG YUNIASTUTI Endang Setyowati ENDANG YUNIASTUTI Erni Irawati Eviani Eviani FAROUQ HEIDAR BARIDO Fitri Aprillia Fitria Roviqowati Fitrotunnisa Al-batul Gani Cahyo Gani Cahyo Handoyo Gani Cahyo Handoyo Hadiandra Harfiansyah Hardjoko, Dwi HASAN BASRI JUMIN Hassan Bashir Hassan Bashir Himawan Joko Riswanda Ida Rumia Manurung Ida Rumia Manurung Ida Rumia Manurung Indah Tri Retnosari Indrawan Fahmi Iswahyudi Iswahyudi Iswahyudi Iswahyudi Iswahyudi Iswahyudi Iswahyudi Iwan Dewangga Joko Prihanto Kevin Putra Setyawan Lixin Zhang MARINA RUZYATI Mochammad Danny Sukardan Muhammad Aji Cahyadi Muhammad Imam Wicaksono Muhammad Imam Wicaksono Muhammad Naufal Adani MUJI RAHAYU Muji Rahayu Muji Rahayu Muji Rahayu MUMTAZ MUAWANAH Nadia Alifia Rahma Nandariyah, Nandariyah Naufal Ghazy Wicaksono Nugraheni Nugraheni NUR AZIZAH USWATUN HASANAH NUR AZIZAH USWATUN HASANAH Nurkholis Majid Okky Talitha ONGKO CAHYONO ONGKO CAHYONO Paramasari Dirgahayu PUJI HARSONO PUJI HARSONO Puji Harsono Purwanto Purwanto Qonita Rahma Dwiyanti Rahayu, Ria Muji Rahmanto Rahmanto Retna Bandriyati Arniputri Retna Bandriyati Arniputri Retna Bandriyati Arniputri Retna Bandriyati Arniputri Retna Bandriyati Arniputri Riandy Adhitya Rina Puji Lestari RIZKA NOVI SESANTI S.Pd. M Kes I Ketut Sudiana . Saat Egra Saras, Anindya Satya Primasasta Zulhivan SELVIA SUTRIANA SETIYANINGSIH, ARY SHOLAHUDDIN SHOLAHUDDIN Sigit PRASTOWO Siti Mardhika Sari Sri Hartati SRI HARTATI Sri Hartati Sri Hartati Sri Hartati Sri Hartati Sri Hartati Sri Hartati Sri Hartati Sri Mulyani SUDADI SUDADI Sudarmi Sudarmi Sulandjari Sulandjari, Sulandjari Supriyono Supriyono SUSANTI INDRIYA WATI TRI JOKO SANTOSO Tyas Alivia Wulandari Venty Suryanti Wartoyo Wartoyo Zuraida Anniswa Bachtiar