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Microclimate modification through shading and watering frequency treatments as efforts for ex situ conservation of pule pandak (Rauvolfia serpentina) SAMANHUDI, SAMANHUDI; PURWANTO, EDI; SULANDJARI, SULANDJARI; SETIYANINGSIH, ARY
Asian Journal of Agriculture Vol 1 No 01 (2017)
Publisher : Society for Indonesian Biodiversity & Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (216.983 KB) | DOI: 10.13057/asianjagric/g010107

Abstract

Samanhudi, Purwanto E, Sulandjari, Setiyaningsih A. 2017. Microclimate modification through shading and watering frequency treatments as efforts for ex situ conservation of pule pandak (Rauvolfia serpentina). Asian J Agric 1: 35-39. Pule pandak (Rauvolfia serpentina Benth) is one of the rare medicinal plants. Exploration of nature continues to be done to meet the needs of the drug. That is why proper cultivation technique for pule pandak is required, by giving appropriate shading level and watering frequency to the growth and yield of pule pandak. The research aimed to examine the effect of shading and watering frequency on the growth and yield of pule pandak. The study was conducted from February to July 2009 in BBP Mondromino, Pokoh Village, Wonogiri at 141 meters above sea level. The experimental Design used in this research was a Split Plot Design consisted two factors and three repetitions. The first factor (main plot) was shading level consisted of three levels: 55%, 65%, and 75%. The second factor (sub plot) was watering frequency consisted of three levels: once three days, once five days and once seven days. Data were subjected to analysis of variance, followed by post hoc test of Duncan Multiple Range Test at 5% level. The reserpine content was analyzed descriptively. The results showed that no interaction between shading level and watering frequency on all variables. The shading level caused no significant effect on growth and yield of pule pandak except the leaf area. Watering frequency caused no significant effect on growth and yield of pule pandak except the chlorophyll content. The results showed that plants treated with watering frequency of five-day interval produced the highest chlorophyll content. Plants treated with shading level of 65% and watering frequency once five days produced the highest reserpine content.
Pertumbuhan In Vitro Tribulus terrestris dengan Perlakuan Indole Butyric Acid (IBA) dan Benzyl Amino Purine (BAP). Samanhudi Samanhudi; Bambang Pujiasmanto; Ahmad Yunus; Nurkholis Majid
AGRIUM: Jurnal Ilmu Pertanian Vol 24, No 1 (2021)
Publisher : UNIVERSITAS MUHAMMADIYAH SUMATERA UTARA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30596/agrium.v23i2.6916

Abstract

Tribulus terrestris merupakan salah satu tanaman obat yang mempunyai beragam manfaat antara lain sebagai diuretik (peluruh kencing), meningkatkan kadar hormon testoteron, memulihkan vitalitas dan menambah kebugaran. Hambatan dari budidaya tanaman ini secara konvensional adalah relatif rendahnya persentase daya kecambah, bentuk bijinya yang kecil dan budidaya yang tergantung pada musim tertentu. Perbanyakan tanaman dengan menggunakan biji secara in vitro merupakan solusi yang dapat digunakan untuk memperbanyak tanaman ini. Penelitian bertujuan untuk mengetahui pertumbuhan in vitro T. terrestris dengan perlakuan IBA dan BAP. Penelitian menggunakan Rancangan Acak Lengkap (RAL) yang disusun secara faktorial. Faktor pertama adalah konsentrasi IBA dan faktor kedua adalah konsentrasi BAP. Analisis data dilakukan dengan uji DMRT dan deskriptif. Hasil penelitian menunjukkan adanya interaksi pemberian IBA 1 ppm + BAP 1 ppm menghasilkan tinggi tunas tertinggi dan jumlah akar terbanyak, interaksi pemberian IBA 1 ppm + BAP 1 ppm dan IBA 1,5 ppm + BAP 2 ppm menginduksi saat muncul akar tercepat. Pemberian IBA secara tunggal tidak mempengaruhi pertumbuhan eksplan T. terrestris, pemberian IBA 0,5 ppm dikombinasikan dengan BAP 2 ppm dapat menginduksi panjang akar terpanjang. Pemberian BAP 1 ppm dapat menginduksi saat muncul tunas tercepat, saat muncul daun tercepat dan jumlah daun terbanyak.
MULTIPLIKASI Aquilaria malaccensis DENGAN NAA DAN RAGI PADA KULTUR IN VITRO Samanhudi Samanhudi; Amalia Tetrani Sakya; Edi Purwanto; Indah Tri Retnosari
Jurnal Pemuliaan Tanaman Hutan Vol 15, No 1 (2021): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2021.15.1.51-59

Abstract

Multiplication of Aquilaria malaccensis with naa and yeast growth regulators on in vitro culture. This study aims to obtain the best concentration of NAA and yeast extract for multiplication of agarwood on in vitro culture. This research was conducted from January to October 2020 at the Laboratory of Plant Physiology and Biotechnology, Faculty of Agriculture, Universitas Sebelas Maret, Surakarta. The experimental design used was a factorial completely randomized design (CRD) with two factors, namely NAA (0 ppm; 0.1 ppm; 0.2 ppm; and 0.3 ppm) and yeast extract (0 mg/l, 700 mg/l, 800 mg/l, and 900 mg/l).(+) The results showed that the combination of 0 ppm NAA and 900 mg/l yeast increased the number of shoots of A. malaccensis explants with the highest average number of 3.67 shoots. A single NAA concentration of 0 ppm was able to increase the number of leaves of explants of A. malaccensis with the highest average leaf rate of 24.5 leaves. A single yeast concentration of 0 mg/l was able to increase the number of leaves of A. malaccensis explants with an average of 22 leaves.
Comparison between the electrical conductivity method and radicle emergence test as a rapid test of sorghum seed vigor Nugraheni, Nugraheni; Pujiasmanto, Bambang; Samanhudi, Samanhudi; Sakya, Amalia Tetrani
Kultivasi Vol 22, No 2 (2023): Jurnal Kultivasi
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kultivasi.v22i2.46547

Abstract

Sorghum (Sorghum bicolor L. Moench) belongs to multipurpose crops. All parts of sorghum can be used both as main products and derivative products; some derivative products produced from the sorghum plant consist of sugar, bioethanol, biomass, handicraft raw materials and starch. This study aims to determine the time required for testing the vigor and viability of sorghum seeds using the electrical conductivity and radicle emergence methods. The study was conducted in two experimental stages using a completely randomized design. The first experiment consisted of two factors: the number of sorghum seeds (40, 70 and 100 grains) and the volume of soaking water (75, 100, 125, and 150 ml). The second experiment consisted of two factors: research method (germination/control method, electrical conduction method, and radicle emergence method) and varieties (consisted of Numbu, Kawali, Suri 3, and Suri 4). The first experiment's results showed that the best electrical conductivity method on Suri 4 varieties were 40 seeds and 150 ml water volume. The electrical conductivity value is negatively correlated with Germination capacity, vigor index, growth of speed, maximum growth potential, and sprout growth rate. Time needed for Electrical Conductivity method in this study was three days. The germination period of 96 hours gives the best results on the radicle emergence of sorghum seed varieties. Vigor index, growth of speed, germination capacity, and maximum growth potential are positively correlated with the value of radicula emergence.Keywords: Electrical Conductivity, Radicle emergence, Vigor, Viability, Germination
Studies of Shading Levels and Nutrition Sources on Growth, Yield and Andrographolide Content of Sambiloto (Andrographis paniculata Ness) Purwanto, Edi; Samanhudi, Samanhudi; Sudarmi, Sudarmi
AGRIVITA Journal of Agricultural Science Vol 33, No 3 (2011)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v33i3.73

Abstract

Growth and biochemical content of medicinal crops are influenced by agroecosystems characteristics . The objective of this research was to determine the optimum shading level and type of fertilizer as sources of nutrition on the growth, yield, and andrographolide content of sambiloto. The experiment used Split Plot Design with basic design of Randomized Complete Block Design arranged with two treatment factors, with three replications. The first factor as the main plot was shading levels, namely without shading, 25% shading, 50% shading, and 75% shading. The second factor as the sub plot was sources of nutrition reprented by type of fertilizer, namely NPK fertilizer, cow stable fertilizer, and compost fertilizer. The result of research indicated that shading level and the kind of nutrition influenced some growth and yield variables such as number of leaves, number of branches, plant height, plant dry weight and simplisia weight, and andrographolide content. Interaction of shading level at 25% and straw compost fertilizer performed best in growth characteristics, while the highest andrographolide content resulted from the treatment combination of 50% shading level and straw compost fertilizer.Keywords: fertilizer,nutrition, shading, andrographolide, Andrographis paniculata
In Vitro Propagation of Temulawak (Curcuma xanthorrhiza Roxb.) Samanhudi, Samanhudi; Yunus, Ahmad; Pujiasmanto, Bambang; Saras, Anindya
Jurnal Jamu Indonesia Vol. 2 No. 2 (2017): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v2i2.30

Abstract

Problems encountered in the development of herbal medicine industry is that most of the raw materials (80%) came from the forest or natural habitats and the rest (20%) of the results of traditional cultivation. The research is focused to get the propagation techniques of temulawak by in vitro using various combinations of IBA and BAP concentration, each consisting of 0, 1, 2, 3, 4 ppm. The purpose of this research is to gain a plant propagation technique of temulawak through the use of IBA and BAP concentration so as to provide a quality seeds. The results showed that the highest shoots obtained at 1 ppm IBA and 3 ppm BAP treatment with a buds height of 15.9 cm. Treatment of 0 ppm IBA and 4 ppm BAP produces the highest buds number by 4 buds. While the highest number of leaves obtained in 2 ppm IBA and 3 ppm BAP treatment with total of 18 leafs. The highest length of leaf obtained in the treatment of 3 ppm IBA and 2 ppm BAP with a length of 14.6 cm. Variable highest number of roots was obtained at treatment of 4 ppm IBA and 1 ppm BAP with root number exceeds 30 pieces with very many hair roots and the highest root length was obtained at treatment of 2 ppm IBA and 0 ppm BAP with a length of 15.6 cm. Based on these results it can be concluded that the IBA and BAP treatment at various concentrations can affect the propagation and growth of temulawak explants.
Diversifikasi Tanaman Pekarangan Dengan Tanaman Alpukat Untuk Meningkatkan Gizi Keluarga Hartati, Sri; Yunus, Ahmad; Nandariyah, Nandariyah; Yuniastuti, Endang; Pujiasmanto, Bambang; Purwanto, Edi; Samanhudi, Samanhudi; Sulandjari, Sulandjari; Ratriyanto, Adi; Prastowo, Sigit; Manurung, Ida Rumia; Suryanti, Venty; Susilowati, Ari; Artanti, Anif Nur; Mulyani, Sri; Dirgahayu, Paramasari
SEMAR (Jurnal Ilmu Pengetahuan, Teknologi, dan Seni bagi Masyarakat) Vol 11, No 2 (2022): November
Publisher : LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/semar.v11i2.61199

Abstract

Alpukat (Persea americana) merupakan buah yang sudah sangat dikenal dan digemari oleh masyarakat. Alpukat diketahui memiliki khasiat sebagai antioksidan, antidiabetik dan efek hipolipidemik. Nutrisi yang terkandung dalam buah alpukat mampu membantu memenuhi kebutuhan nutrisi harian tubuh. Namun, cara budidaya tanaman Alpukat  sangat berpengaruh pada khasiat atau kualitas dan kuantitas dari produk yang dihasilkan. Sehingga diperlukan pemahaman tentang cara budidaya tanaman Alpukat kepada masyarakat. Tujuan yang ingin dicapai dalam kegiatan ini adalah agar masyarakat lebih memahami cara budidaya tanaman alpukat sebagai tanaman pekarangan dan mengetahui manfaat buah Alpukat dalam meningkatkan gizi keluarga. Kegiatan pengabdian sudah dilaksanakan dengan metode penyuluhan dengan penyampaian materi tentang budidaya tanaman Alpukat, manfaat Alpukat, serta berbagai olahan Alpukat sedangkan pada akhir sesi dilakukan pemberian bibit Alpukat serta pupuk pada masing-masing peserta. Kegiatan yang sudah terlaksana ini bermanfaat untuk menambah pengetahuan dan keterampilan masyarakat khususnya masyarakat Kelurahan Wonokarto tentang budidaya tanaman Alpukat dan keanekaragaman manfaat Alpukat untuk kesehatan.Kata Kunci: alpukat; budidaya; penyuluhan; pupuk
Effect of Dolomite and NPK on The Growth and Yield of off-Season Shallots Iswahyudi Iswahyudi; Eddy Tri Haryanto; Supriyono Supriyono; Fitrotunnisa Al-batul; Samanhudi Samanhudi; Erni Irawati; Muji Rahayu; Edi Purwanto
Tropical Plantation Journal Vol 5, No 1 (2026): TROPICAL PLANTATION JOURNAL
Publisher : Akademi Komunitas Perkebunan Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56125/tpj.v5i1.74

Abstract

Shallot is a high-value horticultural commodity. Its productivity often declines due to suboptimal soil conditions, such as low pH and imbalanced nutrient availability, especially when cultivated off-season. This study aimed to determine the optimal combination of dolomite and NPK (Nitrogen, Phosphorus, Potassium) fertilizer for the growth and yield of shallot cultivated in the off-season. The research was conducted in Ngringo Village, Jaten District, Karanganyar Regency from February-April 2025. The method used was a factorial Completely Randomized Design (CRD) with two factors and three replications. The first factor was dolomite doses (0; 2000; and 4000 kg.ha-1) and NPK fertilizer doses (250; 375; and 500 kg.ha-1). Observed parameters were plant height, number of leaves, fresh and dry biomass weight, fresh and dry bulb weight, number of bulbs per plant, and bulb diameter. Data were analyzed by analysis of variance (ANOVA) at a 95% significance level and continued with Duncan’s Multiple Range Test (DMRT) with 95% significance level when significant differences were found. The results showed no significant interaction between dolomite and NPK fertilizer on shallot growth and yield. Dolomite at 2000 kg.ha-1 significantly increased the number of leaves, while NPK fertilizer had no significant effect. However, 250 kg.ha-1 of NPK fertilizer produced similar results to higher doses, indicating potential fertilizer efficiency. Environmental conditions such as high humidity and low light intensity likely influenced treatment effectiveness.