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All Journal Indonesian Journal of Tropical and Infectious Disease 2-TRIK: TUNAS-TUNAS RISET KESEHATAN An Idea Health Journal
Eddy Wasito, Eddy
Microbiology Department, Medical Faculty, Universitas Airlangga
Dentistry Earth & Planetary Sciences Health Professions Medicine & Pharmacology Nursing Public Health

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ANALYSIS OF HIV SUBTYPES AND CLINICAL STAGING OF HIV DISEASE/AIDS IN EAST JAVA Ismail, Yulia; Soetjipto, Soetjipto; Wasito, Eddy; Nasronudin, Nasronudin
Indonesian Journal of Tropical and Infectious Disease Vol 3, No 2 (2012)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (140.202 KB)

Abstract

Human Immunodeficiency Virus type 1 (HIV-1) known to cause Acquired Immune Deficiency Syndrome (AIDS) disease are divided into several subtypes (A, B, C, D, F, G, H, J, K) and Circulating Recombinant Form (CRF). Different characteristics of subtype of the virus and its interaction with the host can affect the severity of the disease. This study was to analyze HIV-1 subtypes circulatingin HIV/AIDS patients from the East Java region descriptively and to analyze its relationship with clinical stadiums of HIV/AIDS. Information from this research was expected to complement the data of mocular epidemiology of HIV in Indonesia. This study utilited blood plasma from patients who had been tested to be HIV positive who sected treatment to or were reffered to the Intermediate Care Unit of Infectious Disease (UPIPI) Dr. Soetomo Hospital Surabaya from various area representing the East Java regions. Plasma was separated from blood samples by centrifugation for use in the the molecular biology examination including RNA extraction, nested PCR using specific primer for HIV gp120 env gene region, DNA purifying, DNA sequencing, and homology and phylogenetic analysis. Based on the nucleotide sequence of the HIV gp120 env gene, it was found that the most dominant subtypes in East Java were in one group of Circulating Recombinant Form (CRF) that is CRF01_AE, CRF33_01B and CRF34_01B which was also found in Southeast Asia. In the phylogenetic tree, most of HIV samples (30 samples) are in the same branch with CRF01_AE, CRF33_01B and CRF34_01B, except for one sample (HIV40) which is in the same branch with subtype B. HIV subtypes are associated with clinical stadiums (disease severity) since samples from different stages of HIV disease have the same subtype.
BALON JOURNAL PENLIT, JIWA Anak TKW 2021 Wasito, Eddy; Budisaputro, Crismantoro
An Idea Health Journal Vol 2 No 01 (2022): FEBRUARY
Publisher : PT.Mantaya Idea Batara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53690/ihj.v3i01.77

Abstract

This research is a field study with the title Study on the Development of the Social Soul of Women's Child Labor using qualitative research approaches and inductive methods. The expected purpose of the research is to find out how the Development of Social Soul of Women child Labor Children and parenting patterns in Joho Village, Merchandise Subdistrict, Madiun Regency. The problem in this study is how is the development of Social Soul and childcare patterns of Women Workers in Joho Village, Merchandise Subdistrict, Madiun Regency?. Research data will be extracted from primary data sources and pseudo-data sources. While the methods used to collect data are methods of observation, interview and dukumentation. Based on the results of data collection, data analysis and discussion showed that the development of the Social Soul of Women's Child Labor is different from children who are always accompanied by their mothers. Childcare is better done by parents because the child's Social Soul Development will be able to develop more optimally in accordance with the rhythm of mental development and will lead the child to better growth, the child can be more independent and can socialize with the environment and be more responsible. While the development of the Social Soul of Women's Child Labor in general experiences psychological social barriers that will not interfere with their developmental tasks.
ANALYSIS OF HIV SUBTYPES AND CLINICAL STAGING OF HIV DISEASE/AIDS IN EAST JAVA Ismail, Yulia; Soetjipto, Soetjipto; Wasito, Eddy; Nasronudin, Nasronudin
Indonesian Journal of Tropical and Infectious Disease Vol. 3 No. 2 (2012)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (140.202 KB) | DOI: 10.20473/ijtid.v3i2.204

Abstract

Human Immunodeficiency Virus type 1 (HIV-1) known to cause Acquired Immune Deficiency Syndrome (AIDS) disease are divided into several subtypes (A, B, C, D, F, G, H, J, K) and Circulating Recombinant Form (CRF). Different characteristics of subtype of the virus and its interaction with the host can affect the severity of the disease. This study was to analyze HIV-1 subtypes circulatingin HIV/AIDS patients from the East Java region descriptively and to analyze its relationship with clinical stadiums of HIV/AIDS. Information from this research was expected to complement the data of mocular epidemiology of HIV in Indonesia. This study utilited blood plasma from patients who had been tested to be HIV positive who sected treatment to or were reffered to the Intermediate Care Unit of Infectious Disease (UPIPI) Dr. Soetomo Hospital Surabaya from various area representing the East Java regions. Plasma was separated from blood samples by centrifugation for use in the the molecular biology examination including RNA extraction, nested PCR using specific primer for HIV gp120 env gene region, DNA purifying, DNA sequencing, and homology and phylogenetic analysis. Based on the nucleotide sequence of the HIV gp120 env gene, it was found that the most dominant subtypes in East Java were in one group of Circulating Recombinant Form (CRF) that is CRF01_AE, CRF33_01B and CRF34_01B which was also found in Southeast Asia. In the phylogenetic tree, most of HIV samples (30 samples) are in the same branch with CRF01_AE, CRF33_01B and CRF34_01B, except for one sample (HIV40) which is in the same branch with subtype B. HIV subtypes are associated with clinical stadiums (disease severity) since samples from different stages of HIV disease have the same subtype.
Pelatihan dengan Teknik Inter-Professional Collaboration untuk Meningkatkan Pengetahuan Petugas Puskesmas tentang Gizi Buruk Kusparlina, Eny Pemilu; Wasito, Eddy
2-TRIK: TUNAS-TUNAS RISET KESEHATAN Vol 12, No 4 (2022): November 2022
Publisher : FORUM ILMIAH KESEHATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/2trik12402

Abstract

Very complex health problems require treatment involving all health professionals. The nutritional status of children is one of the important things to prepare a quality generation. Health education in the form of training is expected to change the competence of collaboration across healthcare professionals. The type of research used is descriptive using a mix method (qualitative and quantitative). This study aims to determine the effect of training on knowledge about collaboration across health professions in handling cases of malnutrition. This research was conducted at the Banjarejo Health Center, Madiun City. The population in this study were all health workers who served at the Banjarejo Health Center. Respondents were 24 people who were obtained by stratified random sampling technique and that was also used as informants. Data was collected in two ways, namely primary data through filling out questionnaires, in-depth interviews with informants and observation. Secondary data in the form of profiles of Health Center, number of health workers and other data needed. The data that has been collected is then tested for normality of the data and presented in the form of a frequency distribution table. Furthermore, because the data on knowledge of malnutrition were not normally distributed, the Wilcoxon test was carried out and knowledge about cross-professional collaboration was normally distributed, a paired t test was performed. For qualitative data, it was analyzed using content analysis and a matrix of informants' statements was compared with the processed quantitative data. Keywords: training; knowledge; poor nutrition; inter-professional collaborationABSTRAK Permasalahan status gizi yang sangat kompleks memerlukan penanganan yang melibatkan ahli gizi kesehatan. Status gizi anak menjadi salah satu hal penting untuk mempersiapkan generasi berkualitas. Pendidikan kesehatan dalam bentuk pelatihan diharapkan mampu mengubah kompetensi kolaborasi lintas profesi petugas kesehatan. Penelitian ini bertujuan untuk mengetahui pengaruh pelatihan terhadap pengetahuan tentang kolaborasi lintas profesi kesehatan pada penanganan kasus gizi buruk. Penelitian ini menerapkan mix method (kualitatif dan kuantitatif). Subyek penelitian adalah 24 petugas kesehatan di Puskesmas Banjarejo, Madiun, yang dipilih dengan teknik stratified random sampling, sekaligus dilibatkan sebagai Informan. Pengumpulan data dilakukan melalui pengisian kuesioner, wawancara mendalam dengan informan dan observasi. Data yang telah dikumpulkan kemudian diuji normalitas data dan disajikan dalam bentuk tabel frekuensi. Selanjutnya dilakukan uji Wilcoxon dan paired sample t-test. Untuk studi kualitatif dilakukan analisis konten dan disusun matriks pernyataan informan untuk perbandingan dengan data kuantitatif.Kata kunci: pelatihan; pengetahuan; gizi buruk; inter-professional collaboration
Co-Authors Budisaputro, Crismantoro Eny Pemilu Kusparlina Nasronudin Nasronudin SOETJIPTO . Yulia Ismail