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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Makara Journal of Science
Nandang Suharna, Nandang
Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences (LIPI), Cibinong, Jakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

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Journal : JURNAL BIOLOGI INDONESIA

 1 
Variasi Genetik Monascus purpureus yang Diisolasi dari Beras Merah Cina Suharna, Nandang
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i3.3470

Abstract

ABSTRACTGenetic Variation of Monascus purpureus Isolated from Chinese Red Rice. An attempt was carried out to detect genetic variation among six Monascus purpureus isolates by RAPD finger printing. The isolates studied were isolated from Chinese red rice (ang-kak) collected from Indonesian markets. Seven types of oligonucleotide 12-mer primers were used in this study. These primers were different from G+C ratio (42%, 50%, 68% and 75%) so as to know the best ratio for DNA amplication. Products of DNA amplication by seven primers were observed such as 158 banding patterns, comprise of 23 common fragments and 135 polimorfic fragments. The result showed that the six isolates were divided into two groups based on DNA banding patterns. The same grouping was also shown by phylogenetic analysis. This analysis indicated two cluster which were different from its genetic distance (49%) and two pairs of isolates were identical to each other. So far, this RAPD analysis proved that there was high genetic variation within the six isolat of M. purpureus. It was assumed that there was a tendency of species separation within those isolates. It is suggested that sequencing analysis on 18S rDNA should be carried out to ensure status of M.purpureus species used in ang-kak production.Key words: Monascus purpureus, variation, genetic, Chinese red-rice, RAPD
Superoxide Dismutase Activity and Ethanol Respiration in a Fungi Resistance to Ethanol Monascus sp. MM Julistiono, Heddy; Suharna, Nandang; Desnora, Beni
JURNAL BIOLOGI INDONESIA Vol 3, No 4 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (456.717 KB) | DOI: 10.14203/jbi.v3i4.3319

Abstract

ABSTRACTMonascus sp. MM was a contaminant fungus isolated from museum specimenpreserved with ethanol 70 %. In order to verify role of superoxide dismutase (SOD) inprotecting cell from ethanol toxicity during ethanol metabolism, SOD activities of Monascussp.MM and a Monmcus sp. NGK, which was isolated fiom fermented red rice (angkak), werecompared. When fungus was grown with glucose, CuIZn-SOD activity of Monascus sp., MMwas 7.1 times of thht of Monascus sp. NGK. Whereas in ethanol medium, CuIZn-SOD activityof Monarcus sp. MM was 24.6 times of that in Monascus sp. NGK. Induction of CuIZn-SODMonmcus sp. MM by ethanol was not observed. Compared with Mn-SOD, activity of CuIZn-SOD was markedly important (I 0 times of Mn-SOD when fungi grown with ethanol; 12 timeswhen the fungi grown with glucose). The data indicated that Cu/Zn-SOD might play animportant role in protecting cell fiom ethanol toxicity during ethanol metabolism. Ethanolrespiration rate of Monascus sp. MM was also important since O2 consumption and ethanoldegradation rates were clearly higher than that of Monascus sp. NGK.Keywords: Monarcw sp., superoxide dismutase, respiration, ethanol resistance.
SUPEROXIDE DISMUTASE ACTIVITY AND ETHANOL RESPIRATION IN A FUNGI RESISTANCE TO ETHANOL MONASCUS SP. MM Julistiono, Heddy; Suharna, Nandang; Desnora, Beni
JURNAL BIOLOGI INDONESIA Vol 3, No 4 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i4.3319

Abstract

ABSTRACTMonascus sp. MM was a contaminant fungus isolated from museum specimenpreserved with ethanol 70 %. In order to verify role of superoxide dismutase (SOD) inprotecting cell from ethanol toxicity during ethanol metabolism, SOD activities of Monascussp.MM and a Monmcus sp. NGK, which was isolated fiom fermented red rice (angkak), werecompared. When fungus was grown with glucose, CuIZn-SOD activity of Monascus sp., MMwas 7.1 times of thht of Monascus sp. NGK. Whereas in ethanol medium, CuIZn-SOD activityof Monarcus sp. MM was 24.6 times of that in Monascus sp. NGK. Induction of CuIZn-SODMonmcus sp. MM by ethanol was not observed. Compared with Mn-SOD, activity of CuIZn-SOD was markedly important (I 0 times of Mn-SOD when fungi grown with ethanol; 12 timeswhen the fungi grown with glucose). The data indicated that Cu/Zn-SOD might play animportant role in protecting cell fiom ethanol toxicity during ethanol metabolism. Ethanolrespiration rate of Monascus sp. MM was also important since O2 consumption and ethanoldegradation rates were clearly higher than that of Monascus sp. NGK.Keywords: Monarcw sp., superoxide dismutase, respiration, ethanol resistance.
VARIASI GENETIK MONASCUS PURPUREUS YANG DIISOLASI DARI BERAS MERAH CINA Suharna, Nandang
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i3.3470

Abstract

ABSTRACTGenetic Variation of Monascus purpureus Isolated from Chinese Red Rice. An attempt was carried out to detect genetic variation among six Monascus purpureus isolates by RAPD finger printing. The isolates studied were isolated from Chinese red rice (ang-kak) collected from Indonesian markets. Seven types of oligonucleotide 12-mer primers were used in this study. These primers were different from G+C ratio (42%, 50%, 68% and 75%) so as to know the best ratio for DNA amplication. Products of DNA amplication by seven primers were observed such as 158 banding patterns, comprise of 23 common fragments and 135 polimorfic fragments. The result showed that the six isolates were divided into two groups based on DNA banding patterns. The same grouping was also shown by phylogenetic analysis. This analysis indicated two cluster which were different from its genetic distance (49%) and two pairs of isolates were identical to each other. So far, this RAPD analysis proved that there was high genetic variation within the six isolat of M. purpureus. It was assumed that there was a tendency of species separation within those isolates. It is suggested that sequencing analysis on 18S rDNA should be carried out to ensure status of M.purpureus species used in ang-kak production.Key words: Monascus purpureus, variation, genetic, Chinese red-rice, RAPD
Co-Authors Desnora, Beni Desnora, Beni Evi Triana HEDDY JULISTIONO Rita Dwi Rahayu Soeka, Yati Yulinery, Titin