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All Journal Majalah Obat Tradisional Heart Science Journal Narra J Indonesian Journal of Biomedicine and Clinical Sciences
Firandi Saputra, Firandi
Faculty of Medicine, Universitas Gadjah Mada
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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SELECTIVITY OF PURIFIED EXTRACT FROM THE LEAVES OF Tithonia diversifolia (Hemsley) A.Gray AGAINST HELA CELLS Wahyuningsih, M. Sri Hartati; Syarif, Rul Afiyah; Suharmi, Sri; Murini, Tri; Saputra, Firandi; W, Adiguno Suryo
Majalah Obat Tradisional Vol 18, No 1 (2013)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (259.979 KB) | DOI: 10.14499/mot-TradMedJ18iss1pp%p

Abstract

Tithonia diversifolia (Hemsley) A.Gray is a kind of plant and traditionally used to cure various diseases. The previous research found that T.diversifolia had antiproliferation effect on colon cancer cell (Col2). Sample preparation using biossay guided extraction and partition method, could simplify the active compounds of the extract. Research on the selectivity of purified extract from the leaves of Tithonia diversifolia (Hemsley) A.Gray) against HeLa cells hasn’t been known yet. The aim of this research was to find out the selectivity of purified extract of T.diversifolia’s leaves on HeLa cell compared to vero cell and to find out the Fifty Percent Inhibition Concentration (IC50), and its selectivity index. Extraction of T. diversifolia‘s leaves were done by maceration method using chloroform and methanol. Both of extract were tested by MTT cytotoxic assay on HeLa cells in vitro with serial doses (0,24 - 500μg/mL). Each group was replicated 3 times. The absorbance was read using ELISA at λ 540nm. IC50 was analyzed by probit regression on SPSS 15 for Windows. Partition (purified extract) of the active extract was done using Petroleum eter (PE), and tested by MTT cytotoxic assay on HeLa cells in vitro.  The IC50 methanol extract to HeLa cell in vitro is 1006,99μg/mL, IC50 chloroform extract is 16,61μg/mL.  The IC50 of soluble PE is 325,33μg/mL.  and IC50 of insoluble PE is 3,078μg/mL. The IC50 value of insoluble PE to Vero cell is 80,30 μg/mL. Selectivity index of purified extract (PE insoluble extract) is 26.09. 
Impact of multivessel coronary artery disease on early and late clinical outcome in ST-Segment elevation myocardial infarction patients who underwent percutaneous coronary intervention: insight from Indonesia Damarkusuma, Arditya; Taufiq, Nahar; Bagaswoto, Hendry Purnasidha; Saputra, Firandi; Sukmadja, Daniel; Setianto, Budi Yuli
Indonesian Journal of Biomedicine and Clinical Sciences Vol 56 No 01 (2024)
Publisher : Published by Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/inajbcs.v56i01.12536

Abstract

It is estimated that 15 people for every 1000 Indonesian residents suffer from cardiovascular disease (CVD) including ST-segment elevation myocardial infarction (STEMI). Percutaneous coronary intervention (PCI) is often performed in patients with STEMI. Several factors affect clinical outcome after PCI procedure including multivessel coronary artery disease. This study aimed to measure the impact of multivessel coronary artery disease on the early and late outcomes of STEMI patients undergoing PCI procedures. This was a prospective cohort study on STEMI patients undergoing PCI procedures from the period of August to December 2021. Two expected cohorts were performed i.e. patients who suffered from single-vessel disease (SVD) and patients who suffered from multivessel disease (MVD). Forty six patients with STEMI were enrolled in this study consisting of 24 (52.17%) patients with MVD and 22 (47.83%) patients with SVD. No significant difference in baseline characteristics between MVD and SVD groups was observed (p > 0.05). The MVD group (91.67%) used a more radial percutaneous approach compared with the SVD group (54.55%; p = 0.04). In addition, no significant difference between the SVD group and the MVD group in major adverse cardiovascular events (MACE) and echocardiographic outcome after 90-d follow up was observed (p > 0.05). In conclusion, MVD has similar impacts on early and late clinical outcomes compared with SVD in STEMI patients undergoing PCI procedures.
The effect of invasive strategy versus conservative strategy on mortality in patients with non-ST-elevation myocardial infarction at Sardjito Hospital Yogyakarta Pradhana, Aditya; Bagaswoto, Hendry Purnasidha; Saputra, Firandi; Setianto, Budi Yuli
Heart Science Journal Vol. 6 No. 3 (2025): Advancements in Cardiac Imaging : Unlocking New Perspectives on the Heart Visua
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.hsj.2025.006.03.14

Abstract

BACKGROUND: Acute myocardial infarction (AMI) causes high hospital admission rates and mortality in Indonesia. Previous studies showed that invasive strategies on non-ST-elevation myocardial infarction (NSTEMI) patients can provide more benefit in clinical outcomes, particularly in high- and very high-risk groups. However, this strategy cannot be implemented due to transportation limitations or a lack of available catheterization facilities in some regions. Recent meta-analyses suggested that early invasive strategies (≤24 hours), delayed strategies (>24 hours), or conservative showed no differences significantly in mortality rates from any cause, stroke or cardiovascular death in NSTEMI patients. OBJECTIVES: This study aims to define the impact of invasive strategies on in-hospital mortality in high and very high-risk NSTEMI patients at Sardjito Hospital. METHODS: A retrospective cohort study utilized data from 326 NSTEMI patients who satisfied the inclusion and exclusion criteria, obtained from the Sardjito Cardiovascular Intensive Care (SCIENCE) registry between January 2023 and June 2024. RESULTS: The invasive and conservative method groups did not substantially differ in in-hospital mortality among NSTEMI patients in either unadjusted (p=0.09) or adjusted analysis (p=0.071, OR 2.251, 95% CI [0.932–5.434]). However, a significant difference was found in the high-risk group (p=0.042) but not in the very-high-risk group (p=0.525). Multivariate analysis confirmed Killip classification (p=0.000, OR 4.449, 95% CI [2.195–9.016]) and hs-Troponin T levels (p=0.006, OR 1.000, 95% CI [1.000–1.000]) as independent mortality predictors. CONCLUSION: Invasive management gave high-risk NSTEMI patients a survival advantage over conservative therapy at Sardjito Hospital, but not in the very-high-risk population.
Association of interleukin-6 and C-reactive protein with in-hospital mortality in patients with ST-elevation myocardial infarction undergoing primary percutaneous coronary intervention Rachman, Adelia U.; Saputra, Firandi; Bagaswoto, Hendry P.; Setianto, Budi Y.
Narra J Vol. 6 No. 2 (2026): August 2026
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v6i2.3085

Abstract

Inflammation contributes substantially to the pathogenesis of acute coronary syndromes (ACS), and interleukin-6 (IL-6) and C-reactive protein (CRP) have been proposed as biomarkers of adverse outcomes. The aim of this study was to evaluate the associations of IL-6 and CRP with in-hospital mortality among patients with ST-elevation myocardial infarction (STEMI) undergoing primary percutaneous coronary intervention (PCI). This prospective cohort study enrolled adult patients with STEMI who underwent primary PCI at Dr. Sardjito General Hospital, Yogyakarta, Indonesia, in 2023. A single blood sample for IL-6 and CRP measurement was collected within 24 hours after PCI. In-hospital mortality was recorded during hospitalization. Receiver operating characteristic analysis identified optimal cut-off values, and multivariable logistic regression was performed to adjust for potential confounders. In-hospital mortality occurred in 6 patients (12.8%). In univariate analysis, higher IL-6 and CRP levels were associated with in-hospital mortality. IL-6 ≥84.60 pg/mL showed an area under the curve (AUC) of 0.776, sensitivity of 66.7%, and specificity of 82.9% (p=0.007), whereas CRP ≥31.35 mg/L showed an AUC of 0.748, sensitivity of 83.3%, and specificity of 68.3% (p=0.015). However, after adjustment for confounding variables in separate multivariable models, neither IL-6 nor CRP remained independently associated with in-hospital mortality. These findings indicate that although elevated IL-6 and CRP levels were associated with in-hospital mortality in unadjusted analyses, their independent prognostic value was not retained after accounting for other clinical and laboratory factors. Further studies with larger sample sizes are needed to clarify the role of these inflammatory biomarkers in risk stratification among patients with STEMI.
Co-Authors Adiguno Suryo W, Adiguno Suryo Bagaswoto, Hendry P. Budi Yuli Setianto Damarkusuma, Arditya Hendry Purnasidha Bagaswoto Mae Sri Hartati Wahyuningsih Nahar Taufiq, Nahar Pradhana, Aditya Rachman, Adelia U. Rul Afiyah Syarif Setianto, Budi Y. Sri Suharmi, Sri Sukmadja, Daniel Tri Murini