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All Journal Jurnal Kedokteran Brawijaya Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Majalah Obat Tradisional Majalah Farmaseutik Media Karya Kesehatan Indonesian Journal of Pharmacology and Therapy
Rul Afiyah Syarif
1) Department Of Pharmacology And Therapy, Faculty Of Medicine Public Health And Nursing, Universitas Gadjah Mada 2) Herbal Medical Center, Faculty Of Medicine Public Health And Nursing, Universitas Gadjah Mada
Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

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SELECTIVITY OF PURIFIED EXTRACT FROM THE LEAVES OF Tithonia diversifolia (Hemsley) A.Gray AGAINST HELA CELLS Wahyuningsih, M. Sri Hartati; Syarif, Rul Afiyah; Suharmi, Sri; Murini, Tri; Saputra, Firandi; W, Adiguno Suryo
Majalah Obat Tradisional Vol 18, No 1 (2013)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (259.979 KB) | DOI: 10.14499/mot-TradMedJ18iss1pp%p

Abstract

Tithonia diversifolia (Hemsley) A.Gray is a kind of plant and traditionally used to cure various diseases. The previous research found that T.diversifolia had antiproliferation effect on colon cancer cell (Col2). Sample preparation using biossay guided extraction and partition method, could simplify the active compounds of the extract. Research on the selectivity of purified extract from the leaves of Tithonia diversifolia (Hemsley) A.Gray) against HeLa cells hasn’t been known yet. The aim of this research was to find out the selectivity of purified extract of T.diversifolia’s leaves on HeLa cell compared to vero cell and to find out the Fifty Percent Inhibition Concentration (IC50), and its selectivity index. Extraction of T. diversifolia‘s leaves were done by maceration method using chloroform and methanol. Both of extract were tested by MTT cytotoxic assay on HeLa cells in vitro with serial doses (0,24 - 500μg/mL). Each group was replicated 3 times. The absorbance was read using ELISA at λ 540nm. IC50 was analyzed by probit regression on SPSS 15 for Windows. Partition (purified extract) of the active extract was done using Petroleum eter (PE), and tested by MTT cytotoxic assay on HeLa cells in vitro.  The IC50 methanol extract to HeLa cell in vitro is 1006,99μg/mL, IC50 chloroform extract is 16,61μg/mL.  The IC50 of soluble PE is 325,33μg/mL.  and IC50 of insoluble PE is 3,078μg/mL. The IC50 value of insoluble PE to Vero cell is 80,30 μg/mL. Selectivity index of purified extract (PE insoluble extract) is 26.09. 
ytotoxic effect of n-hexane insoluble fraction obtained from kloroform extract of kembang bulan leaves [Tithonia diversifolia (Hemsley) A. Gray] on HeLa cells and mechanism detection by Hoechst 33342 Zany sari Vindawati, Rita Rakhmawati, Mae S.H Wahyuningsih, Rul Afiyah Syarif,
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 39, No 03 (2007)
Publisher : Universitas Gadjah Mada

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Abstract

Background: Tithonia diversifolia (Hemsley) A.Gray is a plant traditionally used to cure various diseases. The previous study indicated that n-hexane insoluble fraction of chloroform extract of T. diversifolias leaves showed cytotoxic effect on Hela cells. Separation of n-hexane insoluble fraction into several fractions was conducted in order to simplify the components. Therefore, compounds responsible for cytotoxiceffect and their mechanism of action can be determined. .Objectives: To determine fraction having the most cytotoxic effect and its mechanism of action obtained from n-hexane insoluble fractions of T.diversifolia leaves on Hela cells.Materials and methods: Fractionation of n-hexane insoluble fraction from chloroform extract of T. diversifolia leaves was done by using vacuum liquid chromatography. By MTT assay, the cytotoxic effect of fractions on Hela cells in vitro was determined. and doxorubicin was used as positive control. Each group was tested for 3 times In = 3) and the growth inhibition percentage of Hela cells was counted. The data was analyzed by probit regression, and mechanism of action of the active fraction is conducted by staining with hoechst 33342.Results: Fractionation gave five fractions IF1-5). The ICso levels obtained of those fractions were : F1 having ICso = 32.168 :!: 12.513; F2 = 11.416 :!: 0.535; F3 III = 6.223 :!: 1.303; F4 = 94.308 :!: 7.843; and F5 = 41.740 :!: 6.381Ig/ml; while the ICso value of doxorubicin was 0.241 :!: 0.170 Iglml. Conclusions: Fraction 3 of the n-hexane insoluble displayed the highest cytotoxic effect on Hela cells with ICso= 6.223:!:: 1.303 Iglml. Fraction 3 at the concentration of 3.93 uglml caused chromatin condensation and fragmentation of DNA on Hela cells.Key words: T. diversifolia, cytotoxic effect. Hela cells, insoluble n-hexane fractions
Efek Kurkumin terhadap Sekresi Estrogen dan Ekspresi Reseptor Estrogen β Kultur Sel Granulosa Babi Folikel Sedang Syarif, Rul Afiyah; Soejono, Sri Kadarsih; Meiyanto, Edy; Wahyuningsih, Mae Sri Hartati
Jurnal Kedokteran Brawijaya Vol 29, No 1 (2016)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2016.029.01.7

Abstract

Kurkumin merupakan senyawa yang diisolasi dari Curcuma longa L. Secara empirik C. longa L dikonsumsi masyarakat selama folikulogenesis untuk mencegah kehamilan. Pertumbuhan dan perkembangan sel granulosa tergantung pada FSH, LH, PGF2α, estrogen dan reseptor estrogen β (ERβ). Penelitian ini bertujuan untuk mengkaji efek kurkumin terhadap sekresi estrogen dan ekspresi ERβ pada sel granulosa babi folikel sedang yang dirangsang FSH, LH dan PGF2α. Penelitian ini merupakan penelitian eksperimental laboratorium dengan rancangan post test-only control group. Sel granulosa diisolasi dari folikel ukuran sedang ovarium babi dan disubkultur dalam medium kultur. Penelitian  dilakukan pada  16 kelompok yang terbagi dalam 4 kelompok perangsangan (sel granulosa tidak dirangsang apapun, dirangsang FSH atau LH atau PGF2). Empat kelompok perangsangan dibagi menjadi 4 kelompok perlakuan yaitu kelompok tidak diberi perlakuan dan kelompok diberi kurkumin 3 peringkat konsentrasi. Kadar estrogen dan ekspresi ERβ sel granulosa dianalisa secara enzyme immuneassay. Kadar estrogen dan ekspresi ERβ pada kelompok yang diberi kurkumin tidak berbeda bermakna dengan kelompok kontrol (p>0,05). Kadar estrogen dan ekspresi ERβ pada kelompok yang dirangsang FSH atau LH dan diberi kurkumin berkonsentrasi rendah lebih rendah bermakna daripada tanpa diberi kurkumin (p<0,05). Kadar estrogen sel granulosa yang dirangsang PGF2α dan diberi kurkumin lebih tinggi bermakna daripada tanpa kurkumin (p<0,05), dan tidak ada perbedaan yang bermakna ekspresi ERβ antara kelompok yang dirangsang PGF2α dan diberi kurkumin dengan kelompok tanpa kurkumin (p>0,05). Kurkumin mampu menurunkan estrogen dan ekspresi ERβ sel granulosa yang dirangsang FSH atau LH dari folikel babi ukuran sedang. Dengan demikian, kurkumin dapat mengganggu folikulogenesis dan berpotensi sebagai agen antifertilitas.
ytotoxic effect of n-hexane insoluble fraction obtained from kloroform extract of kembang bulan leaves [Tithonia diversifolia (Hemsley) A. Gray] on HeLa cells and mechanism detection by Hoechst 33342 Mae S.H Wahyuningsih, Rul Afiyah Syarif, Zany sari Vindawati, Rita Rakhmawati
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 39, No 03 (2007)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: Tithonia diversifolia (Hemsley) A.Gray is a plant traditionally used to cure various diseases. The previous study indicated that n-hexane insoluble fraction of chloroform extract of T. diversifolia's leaves showed cytotoxic effect on Hela cells. Separation of n-hexane insoluble fraction into several fractions was conducted in order to simplify the components. Therefore, compounds responsible for cytotoxiceffect and their mechanism of action can be determined. .Objectives: To determine fraction having the most cytotoxic effect and its mechanism of action obtained from n-hexane insoluble fractions of T.diversifolia leaves on Hela cells.Materials and methods: Fractionation of n-hexane insoluble fraction from chloroform extract of T. diversifolia leaves was done by using vacuum liquid chromatography. By MTT assay, the cytotoxic effect of fractions on Hela cells in vitro was determined. and doxorubicin was used as positive control. Each group was tested for 3 times In = 3) and the growth inhibition percentage of Hela cells was counted. The data was analyzed by probit regression, and mechanism of action of the active fraction is conducted by staining with hoechst 33342.Results: Fractionation gave five fractions IF1-5). The ICso levels obtained of those fractions were : F1 having ICso = 32.168 :!: 12.513; F2 = 11.416 :!: 0.535; F3 III = 6.223 :!: 1.303; F4 = 94.308 :!: 7.843; and F5 = 41.740 :!: 6.381Ig/ml; while the ICso value of doxorubicin was 0.241 :!: 0.170 Iglml. Conclusions: Fraction 3 of the n-hexane insoluble displayed the highest cytotoxic effect on Hela cells with ICso= 6.223:!:: 1.303 Iglml. Fraction 3 at the concentration of 3.93 uglml caused chromatin condensation and fragmentation of DNA on Hela cells.Key words: T. diversifolia, cytotoxic effect. Hela cells, insoluble n-hexane fractions
SELEKTIVITAS EKSTRAK TERPURIFIKASI DAUN Tithonia diversifolia (Hemsley) A.Gray TERHADAP SEL HELA M. Sri Hartati Wahyuningsih; Rul Afiyah Syarif; Sri Suharmi; Tri Murini; Firandi Saputra; Adiguno Suryo W
Majalah Obat Tradisional Vol 18, No 1 (2013)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (259.979 KB) | DOI: 10.22146/tradmedj.7757

Abstract

Tanaman kembang bulan [Tithonia diversifolia (Hemsley) A.Gray] merupakan salah satu tanaman yang digunakan dalam pengobatan tradisional untuk berbagai penyakit. Penelitian sebelumnya menyebutkan bahwa kembang bulan memiliki efek antiproliferasi pada sel kanker kolon (Col2). Ekstraksi dan partisi termonitor dengan uji aktivitas merupakan metode untuk mengambil senyawa aktif yang terkandung dalam ekstrak. Penelitian tentang selektivitas ekstrak terpurifikasi dari ekstrak aktif daun Kembang bulan terhadap sel HeLa belum pernah diteliti sebelumnya. Tujuan penelitian ini untuk mengetahui selektivitas ekstrak terpurifikasi daun Kembang Bulan pada sel HeLa dibandingkan dengan sel Vero dan menentukan nilai IC50 serta indeks selektivitasnya. Daun kembang bulan diekstraksi menggunakan pelarut Kloroform dan Metanol. Kedua ekstrak diuji efek sitotoksiknya pada sel Hela dengan beberapa konsentrasi (0,12 s/d 250μg/mL) menggunakan metode MTT (Mosmann, 1983). Pembacaan densitas optik dengan ELISA plate reader. Persentase kematian sel dihitung dan dianalisis dengan menggunakan regresi probit pada program SPSS 15 for Windows. Setelah diketahui nilai IC50 kedua ekstrak  tersebut, dipilih salah satu yang memiliki IC50 lebih kecil kemudian dipurifikasi dengan Petroleum Eter (PE) dan diuji kembali pada sel HeLa, sari yang aktif diuji juga selektivitasnya dengan sel Vero, kemudian dihitung nilai IC50 nya. Nilai IC50 ekstrak metanol sebesar 1006,99μg/mL, ekstrak Kloroform sebesar 16,61μg/mL. Nilai IC50 sari larut PE sebesar 325,331μg/mL dan IC50 sari tidak larut PE sebesar 3,078μg/mL dan nilai IC50 pada sel vero adalah 80,30μg/mL. Nilai indeks selektivitas ekstrak terpurifikasi (sari tidak larut PE) adalah 26.09. 
Heme Polymerization Inhibition by Tithonia diversifolia (Hemsley) A.Gray Leaves Fractions as Antiplasmodial Agent and Its Cytotoxicity on Vero Cells Rul Afiyah Syarif; Mustofa Mustofa; Ngatidjan Ngatidjan; Mae Sri Hartati Wahyuningsih
Majalah Obat Tradisional Vol 23, No 3 (2018)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (280.522 KB) | DOI: 10.22146/mot.36476

Abstract

Previous research revealed that the extracts and fractions of Tithonia diversifolia (Hemsley) A.Gray leaves had antiplasmodial activity in vitro. For further development as an antiplasmodial agent, the mechanisms of action and safety of compounds are important to disclose. Heme polymerization inhibition is one of the main targets of antiplasmodial action. The aim of the study was to investigate the activity of T. diversifolia fractions in inhibiting heme polymerization and its cytotoxic effect on Vero cells. Heme polymerization inhibition assay from Bassilico and cytotoxic test on Vero cell using MTT method were conducted for three fractions (F5, F6, and F7) of T. diversifolia leaves. The inhibitory activity of heme polymerization expressed as IC50 and cytotoxicity effect expressed as CC50 were determined by probit analysis. The best heme polymerization inhibition activity was F5 with IC50 = 162.20 ± 57.81 μg/mL followed by F6 and F7 with IC50 216.30 ± 26.56 and 231.54 ± 44.26 μg/mL respectively. All the fractions had a low cytotoxic effect with CC50 for F5, F6, and F7 were over than 100, 34.81 ± 9.94 and 56.26 ± 6.73 μg/mL, respectively and the toxicity index fraction is below 10 or categorized as low selectivity. Conclusion: The fraction of T. diversifolia inhibited heme polymerization in vitro and had low cytotoxic effect on Vero cells but no selective toxicity. Further research using pure compounds may improve its selectivity.
Ethanolic Extract of Pluchea indica Less leaf Increases Serum Growth Hormone in Lactating Rats Rul Afiyah Syarif; Nungki Anggorowati; Mia Munawaroh; Mae Sri Hartati Wahyuningsih
Majalah Obat Tradisional Vol 26, No 2 (2021)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.62060

Abstract

The objectives of the study were to evaluate the effect of ethanolic extract of P. indica Less leaf (EPI) on serum growth hormone (GH), milk yield, body weight gain of dams, and dam’s organ weight in lactating rats. A total of 25 lactating rats with six pups were randomized and distributed to one of five treatments (control (RO water), standard (domperidone 2,5 mg/kg BW/day), EPI 250 (250 mg/kg BW of EPI), EPI 500 (500 mg/kg BW of EPI), and EPI 750 (750 mg/kg BW of EPI)). The treatment was administered daily starting from the 2nd until the 15th day of lactation. On the 16th day serum growth hormone level, body and organ weight of rats were measured. Serum GH levels in the EPI 750 group (1963.25 ± 360.91 pg/µL) increased significantly compared to the domperidone (409.46 ± 28.80 pg/µL) and the control group (723.40 ± 95.78 pg/µL, p0.05). There was significant body weight gain in the EPI 750 group compared with the domperidone group. There was no significant difference in organ weight gain in each group. The study revealed that ethanolic extract of P. indica Less leaf can increase serum growth hormone in lactating rats.
Penurunan Laktat Dehidrogenase Plasmodium falciparum strain D10 pada pemberian Fraksi Tithonia diversifolia (Hemsley) Rul Afiyah Syarif; Mae Sri Hartati Wahyuningsih; Mustofa Mustofa; Ngatidjan Ngatidjan
Majalah Farmaseutik Vol 17, No 1 (2021)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/farmaseutik.v17i1.57817

Abstract

Penelitian terdahulu menunjukkan bahwa fraksi ke-6 (F6) merupakan fraksi aktif (F-akt) daun Tithonia diversifolia (Hemsley) A.Gray yang menghambat pertumbuhan Plasmodium. Pertumbuhan parasit ini memerlukan energi yang diperoleh dari aktivitas laktat dehidrogenase (LDH). Penelitian ini bertujuan mengkaji aktivitas antiplasmodium fraksi aktif T. diversifolia terhadap kadar LDH kultur P. falciparum. Kultur Plasmodium falciparum strain D10 stadium cincin dibagi menjadi kelompok eritrosit tidak terinfeksi (KTI), eritrosit terinfeksi (KI), dan eritrosit terinfeksi Plasmodium yang diberi F6 (F-akt) T. diversifolia (KI+F-akt) konsentrasi 9,38-150 µg/mL. Kultur diinkubasi 48 jam. Media kultur diukur kadar LDH-nya secara enzimatik. Adanya perbedaan LDH antar kelompok dianalisa dengan Anova. Penghambatan aktivitas LDH (IC50) ditetapkan dengan analisa probit. Kadar LDH kelompok KI (362,33 ± 133,18 U/L) lebih tinggi daripada KTI (270,33 ± 65,85 U/L) (p>0,05). Pemberian F-akt pada KI menyebabkan kadar LDH parasit lebih rendah daripada KI. Kadar LDH parasit yang diberi F-akt konsentrasi 9,38; 18,75; 37,50; dan 150 µg/mL secara berturut-turut adalah 365,5 ± 129,5; 210,5 ± 1,5; 195,5 ± 81,5; dan 111,5 ± 53,5 U/L. Tidak ada perbedaan kadar LDH antar kelompok penelitian (p>0,05). F-akt  T. diversifolia mampu menghambat LDH P. falciparum strain D10 dengan nilai IC50 = 39,22 µg/mL.Kata kunci: Tithonia diversifolia, laktat dehidrogenase, Plasmodium falciparum
Education on Legality, Quality Assurance and Sustainability of 'Jamu Jogorogo' Production in Empowering Women Farming Groups Wicaksono, Arko Jatmiko; Wahyuningsih, Mae Sri Hartati; Syarif, Rul Afiyah; Padmawati, Retna Siwi; Lestari, Beni; Ranti, Imaniar
Media Karya Kesehatan Vol 7, No 2 (2024): Media Karya Kesehatan
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/mkk.v7i2.58490

Abstract

Production of ‘Jamu Jogorogo’ herbal medicine product by the Group of Women Farmers (KWT Singosaren), in Kab. Bantul, Yogyakarta is stagnant after receiving a Dana Keistimewaan Provinsi (Yogyakarta Province’s Special Fund), and has passed a halal certificate. Based on the Ishikawa fishbone analysis, basic knowledge of legal aspects, quality, and marketing strategy. This study was designed to evaluate the effectiveness of an educational strategy applied to the members of KWT Singosaren. This strategy was implemented through a ‘one-day training’ that began with a Pretest, Post-test I, and Post-test II. A total of 80 members of KWT Singosaren were involved in this training, and 59 met the eligible criteria. A Pretest was conducted before the training to get the initial. After the training, participants worked on the Post-test I. Before leaving the training location, all participants were given a pocketbook containing training material for self-learning. Two weeks (14 days) later, they were asked to take the Post-test II, so the participant’s final knowledge could be investigated. As a result, it was found that there is a rising knowledge of 113.56 % and 71.96 % regarding the understanding of ‘quality’ and ‘legality’, respectively. Interestingly, the marketing strategy (product appeal) and sustainable production knowledge rose by 15.25%. Even so, after Post-test II an increase in scores still occurred. This indicates that participants successfully conducted the self-learning process. Post actualization period, we found that the SPP-IRT permits (legal permits for food production for the Home Industry) of 3 (three) herbal products were successfully issued. Keywords: Empowerment, women, effectiveness, knowledge, training.
Acute irritation of tetracyclic c-4-hydroxyphenylcalics [4] pyrogalolarene on skin of albino rabbits Aditya Nugraha; Dwi Aris Agung Nugrahaningsih; Rul Afiyah Syarif; Mia Munawaroh Yuniyanti; Ikhsan Nur Salim; Ratna Sari; Jumina; Eti Nurwening Sholikhah
Indonesian Journal of Pharmacology and Therapy Vol 1 No 1 (2020)
Publisher : Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada and Indonesian Pharmacologist Association or Ikatan Farmakologi Indonesia (IKAFARI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijpther.480

Abstract

C-4-hydroxyphenylcalix[4] pyrogalolarene (Pg4OH) is a synthetic chemical compound that shows strong antioxidant activity. It is potential to be developed as UV skin protector. For topical admission, an acute skin irritation test is being prerequisite to be fulfill. This study was conducted to investigate the irritation effect of Pg4OH on the skin. In vivo study by quasi experiment with posttest only design was carried out by employing 3 adult male albino rabbits. The back hair was shaved at 24 h before the treatment. The Pg4OH was smeared on the bare shaved-skin and left naturally within 4 h. The observation began at 24, 48 and 72 h after exposure. The erythema and edema scores were measured. Calculation by using primary irritation index (PII) was applied. Calculation was categorized with globally harmonized system (GHS) and international organization for standardization (ISO) parameters. As a result, among three rabbits, only one animal shown erythema and edema with very small size (score: 1), on 3 sites, at 72 h. Both the control site and the sample sites gained same score. Two other rabbits were obtained 0 value at each observation location. According to the PII, the observed data was obtained score 0.0093 (very light or negligible). It can be concluded that the tetracyclic compound of Pg4OH does not irritate the skin.
Co-Authors Adiguno Suryo W Adiguno Suryo W, Adiguno Suryo ADITYA NUGRAHA Aditya Nugraha Arko Jatmiko Wicaksono, Arko Jatmiko Beni Lestari Dwi Aris Agung Nugrahaningsih Edy Meiyanto Eti Nurwening Sholikhah Firandi Saputra Firandi Saputra, Firandi Ikhsan Nur Salim Ikhsan Nur Salim Jumina Jumina Jumina Lisma Evareny, Mohammad Hakimi, Retna Siwi Padmawati Mae Sri Hartati Wahyuningsih Mia Munawaroh Mia Munawaroh Yuniyanti Mustofa Mustofa Mustofa Mustofa Ngatidjan Ngatidjan Nungki Anggorowati Ranti, Imaniar RATNA SARI Ratna Sari Rita Rakhmawati, Rita Sri Kadarsih Soejono Sri Suharmi, Sri Tri Murini Tri Murini Yacobus Christian Prasetyo Yuniyanti, Mia Munawaroh