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DIFFERENCES OF UNIVERSAL AND MULTIPLEX PRIMER FOR DETECTION OF DENGUE VIRUS FROM PATIENTS SUSPECTED DENGUE HEMORRHAGIC FEVER (DHF) IN SURABAYA Ansori, Arif; Sucipto, Teguh; Deka, Pemta; Ahwanah, Nur; Churrotin, Siti; Kotaki, Tomohiro; Soegijanto, Soegeng
Indonesian Journal of Tropical and Infectious Disease Vol. 5 No. 6 (2015)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (220.223 KB) | DOI: 10.20473/ijtid.v5i6.594

Abstract

Dengue Hemorrhagic Fever (DHF) is a global health problem in tropical and subtropical regions, as well as endemic in110 countries around the world. Indonesia is one of the largest countries in the region of endemic dengue. In Indonesia, dengue virus infection has become a contagious disease that was very important and was reported in 1968. Many molecular epidemiological approaches have been developed to look for factor that has been assumed as the cause of the increase of prevalence dengue virus infection in the world. The aim of this study is for the detection and determination of serotype of dengue virus in Surabaya. The method used was the technique of Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and Polymerase Chain Reaction (PCR) with specific primers for dengue virus. Samples suspected DHF patients were obtained from various health center and hospital in Surabaya. Results of this research detected negative result for dengue virus in all samples of patients suspected DHF. Negative results caused by dengue virus titers in serum samples of patients who had been dropped due to long storage time and taken after the third day of fever in early period.
Pendampingan Pengelolaan Sampah Berkelanjutan melalui Ecobrick, Ecoprint, dan Bank Sampah Ansori, Arif; Al Haq, Abdul Aziz; Suryadi, Muhammad Abrar; Alfayed, Erico; Lestari, Sri Ayu; Ningsih, Nurwidia; Intan, Melani Nur; Fadila, Sovie; Rambe, Multisari; Masyhur, Laila Sari
MENARA RIAU Vol 19, No 1 (2025): April 2025
Publisher : Lembaga penelitian dan pengabdian kepada masyrakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/menara.v19i1.32580

Abstract

This community service program aimed to address waste management issues through the implementation of ecobrick, ecoprint, and waste bank initiatives. The ecobrick program successfully reduced plastic waste by involving students in creating eco-friendly bricks used to build the village name sign. The ecoprint program, targeting local women's groups (PKK), enhanced their skills in transforming organic waste into valuable artistic products such as leaf-patterned tote bags. Meanwhile, the establishment of a waste bank encouraged residents to sort organic and inorganic waste and promoted collective participation in sustainable waste management. These three programs not only helped reduce environmental pollution but also created new economic opportunities for the community. The results showed increased awareness and engagement among residents in sustainable waste practices, aligning with environmental development goals and community empowerment efforts.