Sri Samsundari, Sri
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EFFECTIVENESS OF EXTRACT SONGGA WOOD (STRYCHNOS LUCIDA) TOWARDS SURVIVAL AND ANTIBODY TITERS ON TILAPIA (OREOCROMIS NILOTICUS) THAT INFECTED STREPTOCOCCUS AGALACTIAE Zubaidah, Anis; Samsundari, Sri; Fitriani, Vivi
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Publisher : University of Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/ijota.v2i2.11136

Abstract

Nile Tilapia or Tilapia Fish (Oreochromis niloticus) is one of commodity with a high number of consumers. But, Streptococcus agalactiae attack caused a decline in production. One way to reduce these diseases by using natural materials of songga wood (Strychnos lucida R.Br) where the songga wood contains many compounds like alkaloid, phenols, flavonoids, triterpenoids, which can inhibit bacterial growth by damaged cell membrane. The results showed that the inhibitory songga wood extract against Streptococcus agalactiae was highly significant, and shows with the highest survival rate of 90% and proved by the increasing antibody titers after adding songga wood extract.Keywords: Bacterial disease, Fish immune, Phytopharmacy, Tilapia
EFFECTIVENESS OF STRYCHNOS LIGUSTRINA BL. EXTRACT AS FEED SUPPLEMENTATION TO INCREASE IMMUNE SYSTEM OF NILE TILAPIA (OREOCHROMIS NILOTICUS) WICH AGAINTS STREPTOCOCCUS AGALACTIAE Zubaidah, Anis; Faidah, Kuny Riski; Samsundari, Sri
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Publisher : University of Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/ijota.v1i1.6082

Abstract

One of the problems of intensive cultivation of tilapia is the attack of Streptococcosis disease caused by Streptococcus agalactiae bacteria. To prevent it is necessary to prevent the use of natural antibiotics that is Strychnine bush extract. The method used in this research is the experimental method using Completely Randomized Design (RAL), then the data is analyzed by using ANAVA. In this research, there were five treatments with various dosages, namely treatment (K: 0%), (A: 5%), (B: 10%), (C: 15%) and (D: 20%), each treatment was repeated four times. The main parameters used are hematocrit level, leukocrit level, leukocyte count and phagocytosis activity. As a supporting parameter is water quality and MIC (Minimum Inhibitation Concentration) test. The results showed that dosage of Strychnine bush extract did not affect the level of hematocrit and leukocrit level, but significant effect on leukocyte count with the highest value at treatment B with 10% dose is 11,9 × 105 cell / mm3. Giving extract also significant effect on phagocytosis activity with the highest phagocytosis value at treatment B with dose of 10% that is 32%.Keywords: Imune system, Streptococcosis, antibiotics, Minimum Inhibitation Concentration
EFFECTIVENESS OF STRYCHNINE BUSH EXTRACT (STRYCHNOS LIGUSTRINA BLUME) AGAINST TO PREVALENCE AND SURVIVAL RATE OF DUMBO CATFISH (CLARIAS GARIEPINUS) INFECTED WITH AEROMONAS HYDROPHILA Zubaidah, Anis; Samsundari, Sri; Hidayaturrahmi, Hidayaturrahmi
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Publisher : University of Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/ijota.v2i1.5601

Abstract

One of the problemsofintensive catfish culture is the attack of Motile Aeromonas Septicemia disease caused by Aeromonas hydrophila bacteria. To prevent it is necessary to prevent the use of natural antibiotics that is strychnine bush extract. The purpose of this research is to know the ability of strychnine bush extract (Strychnos ligustrina Blume) using methanol solvent in inhibiting the growth of Aeromonas hydrophila bacteria and its effect on prevalence and survival rate in Dumbo catfish (Clarias gariepinus). The method used in this research is the exsperimen method by using complete randomized design (RAL). In this study there were five treatments with different doses of treatment (P1: 6%), (P2: 12%), (P3: 18%), (P4: 24%) and (P5: 30%), each treatment was repeated three replications. Furthermore, the data were analyzed using ANAVA and continued with LSD test. The results showed that the giving of strychnine bush extract with different concentrations had a very significant effect on the width of the Aeromonas hydrophila bacterial resistance zone. The lowest inhibition of the treatment (P1: 6%) with a resistance of 1.79 mm. While the highest inhibition of treatment (P5: 30%) with 8.29 mm inhibition power. In prevalenceofdumbo catfish with givingofsongga extract very real effect, treatment with lowest prevalence is treatment (P4: 24%) with prevalence value 23,3% andhighestpervalence that is treatment (P5: 30%) with prevalence value equal to 40 %. In the synthesisofdumbo catfish with the giving of strychnine bush extract have a very real effect, the treatment with lowest stability is treatment (P4: 30%) with 77% syntax value and the highest synthesis of treatment (P5: 24%) with 90% syntax value. This study resulted in the conclusion that the best treatment for prevalence and survival rate is treatment (P4: 24%). As for the higher inhibition power dose strychnine bushextractis used then the higher the power inhibition.