Article Per Year (5 Year)
p-Index From 2020 - 2025
0.562
P-Index
This Author published in this journals
All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences JURNAL SAINS DAN TEKNOLOGI INDONESIA Journal of Aquaculture Management and Technology Jurnal Bioteknologi & Biosains Indonesia (JBBI) Journal of Language, Literature, Social and Cultural Studies
Siti Zulaeha, Siti
Program Studi Budidaya Perairan, Jurusan Perikanan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro
Agriculture, Biological Sciences & Forestry Arts Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Social Sciences Other

Published : 7 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 7 Documents
Search

 1 
PERBANDINGAN TIGA KIT EKSTRAKSI RNA UNTUK ANALISIS TRANSKRIPTOMIKA PADA KELAPA SAWIT (Elaeis guineensis Jacq.) Zulaeha, Siti; Purwoko, Devit; Cartealy, Imam; Tajuddin, Teuku; Karyanti, .; Khairiyah, Hayat
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 6, No 1 (2019): June 2019
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (143.283 KB) | DOI: 10.29122/jbbi.v6i1.3372

Abstract

Comparison of Three RNA Extraction Kits for Transcriptome Analysis of Oil Palm (Elaeis guineensis Jacq.) ABSTRACTObtaining high-quality RNA is very important at an early stage of molecular biology research. To isolate RNA, high skill and caution are required in following laboratory procedures because RNA is easily degraded, especially samples from plant tissue culture. One of the parameters used to check the total RNA quality is RIN (RNA Integrity Number). The aim of this study was to obtain RNA extraction methods on oil palm leaves, callus and somatic embryos that were of good quality and high concentrations for transcriptomic analysis. RNA extraction was carried out using Plant RNA PureLink (Ambion), Genezol RNA Extraction (Geneaid) and RibospinTM Plant (Geneall) kit methods. The results showed that oil palm leaf, callus and somatic embryo RNA were successfully extracted using the RibospinTM (Geneall) kit. Based on the total RNA number of more than 4 μg and the RIN value of more than 7, the extracted RNA could be used in RNA sequencing for transcriptomic analysis. Keywords: callus, oil palm, RNA analysis, RNA quality, somatic embryo ABSTRAKMenghasilkan RNA berkualitas tinggi sangatlah penting pada tahap awal penelitian biologi molekuler. Untuk mengisolasi RNA diperlukan keterampilan dan kehati-hatian tinggi dalam mengikuti prosedur di laboratorium karena RNA lebih mudah terdegradasi, khususnya sampel hasil kultur jaringan tanaman. Salah satu parameter yang digunakan pada pengecekan kualitas RNA total adalah RIN (RNA Integrity Number). Penelitian bertujuan mendapatkan metode ekstraksi RNA pada daun, kalus dan embrio somatik kelapa sawit yang berkualitas baik dan memiliki konsentrasi tinggi untuk analisa transkriptomika.  Ekstraksi RNA dilakukan menggunakan metode kit Plant RNA PureLink (Ambion), Genezol RNA Extraction (Geneaid) dan RibospinTM Plant (Geneall). Hasil menunjukkan bahwa RNA daun, kalus dan embrio somatik kelapa sawit telah berhasil diekstraksi dengan menggunakan kit RibospinTM (Geneall). RNA hasil ekstraksi tersebut dapat digunakan untuk sekuensing RNA dengan tujuan analisis transkriptomika, dilihat dari jumlah total RNA yang lebih dari 4 μg dan nilai RIN lebih dari 7. Kata Kunci: analisis RNA, embrio somatic, kalus, kelapa sawit, kualitas RNA 
PENGARUH PENAMBAHAN ENZIM FITASE PADA PAKAN BUATAN TERHADAP EFISIENSI PEMANFAATAN PAKAN, DAN PERTUMBUHAN IKAN KERAPU BEBEK (Cromileptes altivelis) Zulaeha, Siti; Rachmawati, Diana; Samidjan, Istiyanto
Journal of Aquaculture Management and Technology Volume 4, Nomor 4, Tahun 2015
Publisher : Journal of Aquaculture Management and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (428.697 KB)

Abstract

Ikan kerapu bebek merupakan jenis ikan yang bernilai ekonomis tinggi, prospek pemasaran cukup baik karena diminati konsumen sebagai ikan konsumsi dan ikan hias. Salah satu aspek yang perlu diperhatikan dalam usaha budidaya ikan kerapu khususnya pakan. Pakan merupakan salah satu komponen penting budidaya ikan yang berperan terhadap pertumbuhan dan asupan nutrisi ikan. Penggunaan bahan baku pembuat pakan seperti bungkil kedelai sebagai sumber protein dari bahan nabati. Namun, bungkil kedelai ternyata mengandung asam fitat. Asam fitat yang terkandung dalam pakan dapat menghambat pertumbuhan. Asam fitat tidak dapat terhdirolisis dalam saluran pencernaan ikan oleh karena itu perlu ditambahkan enzim fitase guna memecah asam fitat menjadi inositol dan asam fosfat. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan enzim fitase dan dosis optimal enzim fitase terhadap efisiensi pemanfaatan pakan, dan pertumbuhan ikan kerapu (Cromileptes altivelis). Ikan uji yang digunakan selama penelitian adalah ikan kerapu bebek dengan bobot rata-rata 3±0,55 g/ekor-1 dan padat tebar 1 ekor/l-1. Metode yang digunakan yaitu metode ekperimen dengan menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 3 kali ulangan. Perlakuan dalam penelitian ini adalah penambahan enzim fitase dengan dosis yang berbeda yaitu perlakuan A (tanpa enzim fitase), B (500 mg/kg pakan), C (1000 mg/kg pakan), dan D (1500 mg/kg pakan). Data yang diamati selama penelitian yaitu efisiensi pemanfaatan pakan (EPP), rasio efisiensi protein (PER), laju pertumbuhan relatif (RGR), dan kualitas air. Berdasarkan hasil penelitian menunjukkan bahwa penambahan enzim fitase dalam pakan buatan berpengaruh sangat nyata (P<0,01) terhadap EPP, PER, dan RGR, namun tidak berpengaruh nyata (P>0,05) terharap SR. Dosis optimum enzim fitase 986 mg/kg pakan dapat meningkatkan laju pertumbuhan relatif ikan kerapu bebek maksimal sebesar 2,24%, dan dosis optimum enzim fitase 950 mg/kg pakan mampu menghasilkan efisiensi pemanfaatan pakan maksimal 28,5%. Kualitas air pada media pemeliharaan masih berada pada kisaran yang layak untuk budidaya ikan kerapu bebek.       Cromileptes altivelis is one of high economic value fish, which is good in marketing prospects for demand by consumers as food and ornamental fish. One of aspects that need attention in the business of cultivating Cromileptes altivelis is the feed. Feed is an of important componend in aquaculture, it has a role in growth and nutrient intake of fish. Use of raw material feed such as soybean meal as a protein source of vegetable material . However, soybean meal turned out to contain phytase acid. Phytase acid contained in feed could inhibit growth. Phytase acid can’t be hydrolized in fish digestive therefore need to be added phytase enzyme to break down phytase acid into inositol and phospor acid. The study aim to determine influence of Phytase enzyme additon for artificial feed; and to know the the optimum level of artificial feed on the growth efficiency of Cromileptis altivelis fish. The material used in this reaserch was Cromileptus altivelis fish with average weight 3±0,55g/fish and the method used is complete randomized design test with 4 treatments and 3 replicates. The treatments were feeding dosage: A (without phytase enzyme), B (500 mg/feed), C (1000 mg/feed), and D (1500 mg/feed). Data that observed in research are Efficiency of Feed Utilization (EPP), Protein  Efficiency Ratio (PER), Relative Growth Rate (RGR), and Waters quality. The research showed that phytase enzyme addition in artificial feed very significantly (P <0,01) on the EPP, PER, and RGR, but not significant (P> 0,05) against to SR. The optimum dosage 986 mg/kg of feed can improve Cromileptes altivelis to producing maximum relative growth rate of 2,24%, and the optimum dose of the enzyme phytase 950 mg / kg feed capable to producing maximum feed utilization efficiency of 28,5%. The range of water quality was still on the decent range for culturing. 
Analisis Keragaman Genetik Temulawak (Curcuma xanthorrhiza Roxb.) Menggunakan Penanda Amplified Fragment Length Polymorphism (AFLP) damayanti, dini; tajuddin, teuku; purwoko, devit; zulaeha, siti; suharsono, suharsono
Jurnal Sains dan Teknologi Indonesia Vol. 14 No. 3 (2012)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (197.369 KB) | DOI: 10.29122/jsti.v14i3.923

Abstract

Curcuma xanthorrhiza Roxb, which is well-known as Java turmeric, has been extensively used in pharmaceutical industries in Indonesia. In spite of this commercial value, the identity of this species is commonly mistaken from other similar orange rhizomes Curcuma. Correct identity of these species is vital in pharmaceutical industries. The objective of the study was to determine genetic diversity of 32 accession Curcuma xanthorrhiza Roxb. Genomic DNA was extracted from leaf using Sodium Dodesyl Sulphate (SDS) modification. Amplified fragment length polymorphism (AFLP) was carried out according to the protocol ofAFLPTM plant mapping kit and the final polymerase chain reaction (PCR) products were separated using The Agilent 2100 Bioanalyzer. The number of fragment produced by 12 pairs primer combination of AFLP ranged from 42 to 60 with an average of 52. Data obtained was analyzed by the NTSys program. From the AFLP amplification on 32 DNA samples, it was proven that the accession of Curcuma xanthorrhiza Roxb. had a high degree of diversity. Based on analysis of AFLP and unweighted pair group with arithme average (UPGMA) it was shown that the accession of Curcuma xanthorrhiza Roxb. could be grouped into two cluster at relative ecludian distance of 0.10 (10%). Cluster I for accession from Palembang, Pacitan and Ciamis 2. Cluster II for accession from Makale, Pontianak, Kulonprogo, Mataram, Boyolali, Salatiga, Sumberejo, Bali, P. Seram, Sentolo, Purworejo, Samas Bantul, Ciamis1, Blora, Semarang, Poso, Kalsesl, Tagari, Merapi Farm, Salakaria, NTB, Menoreh, Karang Anyar, Mangunan, Medan, Toraja, dan Solok.
PERBANDINGAN TIGA KIT EKSTRAKSI RNA UNTUK ANALISIS TRANSKRIPTOMIKA PADA KELAPA SAWIT (Elaeis guineensis Jacq.) Zulaeha, Siti; Purwoko, Devit; Cartealy, Imam; Tajuddin, Teuku; Karyanti, .; Khairiyah, Hayat
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 1 (2019): June 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (143.283 KB) | DOI: 10.29122/jbbi.v6i1.3372

Abstract

Comparison of Three RNA Extraction Kits for Transcriptome Analysis of Oil Palm (Elaeis guineensis Jacq.) ABSTRACTObtaining high-quality RNA is very important at an early stage of molecular biology research. To isolate RNA, high skill and caution are required in following laboratory procedures because RNA is easily degraded, especially samples from plant tissue culture. One of the parameters used to check the total RNA quality is RIN (RNA Integrity Number). The aim of this study was to obtain RNA extraction methods on oil palm leaves, callus and somatic embryos that were of good quality and high concentrations for transcriptomic analysis. RNA extraction was carried out using Plant RNA PureLink (Ambion), Genezol RNA Extraction (Geneaid) and RibospinTM Plant (Geneall) kit methods. The results showed that oil palm leaf, callus and somatic embryo RNA were successfully extracted using the RibospinTM (Geneall) kit. Based on the total RNA number of more than 4 μg and the RIN value of more than 7, the extracted RNA could be used in RNA sequencing for transcriptomic analysis. Keywords: callus, oil palm, RNA analysis, RNA quality, somatic embryo ABSTRAKMenghasilkan RNA berkualitas tinggi sangatlah penting pada tahap awal penelitian biologi molekuler. Untuk mengisolasi RNA diperlukan keterampilan dan kehati-hatian tinggi dalam mengikuti prosedur di laboratorium karena RNA lebih mudah terdegradasi, khususnya sampel hasil kultur jaringan tanaman. Salah satu parameter yang digunakan pada pengecekan kualitas RNA total adalah RIN (RNA Integrity Number). Penelitian bertujuan mendapatkan metode ekstraksi RNA pada daun, kalus dan embrio somatik kelapa sawit yang berkualitas baik dan memiliki konsentrasi tinggi untuk analisa transkriptomika.  Ekstraksi RNA dilakukan menggunakan metode kit Plant RNA PureLink (Ambion), Genezol RNA Extraction (Geneaid) dan RibospinTM Plant (Geneall). Hasil menunjukkan bahwa RNA daun, kalus dan embrio somatik kelapa sawit telah berhasil diekstraksi dengan menggunakan kit RibospinTM (Geneall). RNA hasil ekstraksi tersebut dapat digunakan untuk sekuensing RNA dengan tujuan analisis transkriptomika, dilihat dari jumlah total RNA yang lebih dari 4 μg dan nilai RIN lebih dari 7. Kata Kunci: analisis RNA, embrio somatic, kalus, kelapa sawit, kualitas RNA 
An investigation of the teacher’s challenges and strategies in teaching English in a rural area Zulaeha, Siti; Riyanti, Dwi
Journal of Language, Literature, Social and Cultural Studies Vol. 2 No. 2 (2024): July 2024
Publisher : Yayasan Mitra Persada Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58881/jllscs.v2i2.174

Abstract

The purpose of this study was to investigate the challenges faced by an English teacher and the strategies to solve the challenges in teaching English in a rural area, namely Sebangki. This narrative study used interview with an English teacher at SMA Negeri 1 Sebangki. The results of this study indicated that there are two main themes of challenges faced by teacher including the challenges from the teacher consists of distance to school, teaching other subjects, lack of teacher training, and students problems consists of lack of interest, lack of confidence, lack of vocabulary mastery and students’ attendance. To overcome those challenges, the English teacher employed some strategies including using media and strategy in teaching, giving motivation and even giving punishment to the students.
Identification of Gene Candidates in Diterpenoid Biosynthesis of Curcuma longa: An mRNA Sequencing Approach: Identification of Gene Candidates in Diterpenoid Fadhullah, Hafizh; Purwoko, Devit; Zulaeha, Siti; Hanifah, Nurul Fitri; Hartuti, Endah Dwi; Rahmadara, Gemilang; Safarrida, Anna; Reninta, Rikania; Evawati, Evawati; Roza, Irwan; Tajuddin, Teuku
Journal of Tropical Life Science Vol. 14 No. 3 (2024): In Press
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.03.08

Abstract

Curcuma longa is a medicinal plant renowned for its therapeutic properties and potential treatment of cancer. This study focused on the biosynthesis of diterpenoids in the rhizome and leaves of C. longa. The genes responsible for producing these medicinal compounds were analyzed using BLASTx, Gene Ontology (GO) annotation, differential expression, and homology. The substantial dataset was obtained from the National Center for Biotechnology Information (NCBI), comprising 151,730,334 clean reads and 167,264 transcripts for the analysis. The results of the BLASTx analysis were as follows: NR yielded 65.93%, Swiss-Prot yielded 44.52%, and COG yielded 17.35%. Subsequently, GO annotation was performed using Blast2GO, resulting in an annotation rate of 56.79%. Differential expression analysis revealed a total of 636 genes that were significantly differentiated between the rhizome and leaves. The homology analysis resulted in 11 proteins associated with diterpenoid biosynthesis and nine proteins related to CYP450. Approximately three class I proteins were highly expressed in the rhizome. Additionally, seven CYP450 enzymes from the CYP71D and CYP726 subfamilies were identified; three were highly expressed in the rhizome. The expression patterns of these enzymes were similar to the aforementioned three class I diTPSs, indicating their potential involvement in macroditerpenoid biosynthesis in C. longa. These findings provide valuable genomic resources for future functional genomics research on C. longa, facilitating targeted efforts to enhance the production of bioactive compounds.
Identification of Gene Candidates in Diterpenoid Biosynthesis of Curcuma longa: An mRNA Sequencing Approach: Identification of Gene Candidates in Diterpenoid Fadhullah, Hafizh; Purwoko, Devit; Zulaeha, Siti; Hanifah, Nurul Fitri; Hartuti, Endah Dwi; Rahmadara, Gemilang; Safarrida, Anna; Reninta, Rikania; Evawati, Evawati; Roza, Irwan; Tajuddin, Teuku
Journal of Tropical Life Science Vol. 14 No. 3 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.03.08

Abstract

Curcuma longa is a medicinal plant renowned for its therapeutic properties and potential treatment of cancer. This study focused on the biosynthesis of diterpenoids in the rhizome and leaves of C. longa. The genes responsible for producing these medicinal compounds were analyzed using BLASTx, Gene Ontology (GO) annotation, differential expression, and homology. The substantial dataset was obtained from the National Center for Biotechnology Information (NCBI), comprising 151,730,334 clean reads and 167,264 transcripts for the analysis. The results of the BLASTx analysis were as follows: NR yielded 65.93%, Swiss-Prot yielded 44.52%, and COG yielded 17.35%. Subsequently, GO annotation was performed using Blast2GO, resulting in an annotation rate of 56.79%. Differential expression analysis revealed a total of 636 genes that were significantly differentiated between the rhizome and leaves. The homology analysis resulted in 11 proteins associated with diterpenoid biosynthesis and nine proteins related to CYP450. Approximately three class I proteins were highly expressed in the rhizome. Additionally, seven CYP450 enzymes from the CYP71D and CYP726 subfamilies were identified; three were highly expressed in the rhizome. The expression patterns of these enzymes were similar to the aforementioned three class I diTPSs, indicating their potential involvement in macroditerpenoid biosynthesis in C. longa. These findings provide valuable genomic resources for future functional genomics research on C. longa, facilitating targeted efforts to enhance the production of bioactive compounds.
Co-Authors Anna Safarrida, Anna Cartealy, Imam Cartealy, Imam damayanti, dini Diana Rachmawati Dwi Riyanti Evawati Evawati Fadhullah, Hafizh Hanifah, Nurul Fitri Hartuti, Endah Dwi Irwan Roza Istiyanto Samidjan Karyanti, . Karyanti, . Khairiyah, Hayat Khairiyah, Hayat purwoko, devit Purwoko, Devit Rahmadara, Gemilang Reninta, Rikania Suharsono Suharsono Teuku Tajuddin