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All Journal Jurnal Agrotek UMMat
Hasbi Mubarok Suud, Hasbi Mubarok
Program Studi Teknik Mesin Pertanian dan Pangan, Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry

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Pengaruh pemberian thidiazuron dan NAA pada media MS terhadap regenerasi eksplan nodal vanili (Vanilla Planifolia Andrews) secara in vitro Ardyansah, Muhammad Ikrom; Kusbianto, Dwi Erwin; Suud, Hasbi Mubarok; Rosyady, Muhammad Ghufron
Jurnal Agrotek Ummat Vol 11, No 4 (2024): Jurnal Agrotek Ummat
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jau.v11i4.26325

Abstract

Vanilla is a commodity with high economic value that belongs to the Orichidaceae family. One of the problems in vanilla cultivation is unhealthy seedlings and mother plants due to Fusarium oxysporum infection. In vitro plant propagation is an alternative because it uses a small part of the plant on sterile media so that it becomes a healthy plant. The objective of this study was to determine the effect of thidiazuron and NAA on the growth response of vanilla (Vanillaplanifolia Andrews) node explants in vitro. This research method uses RAL, which consists of two factors. Thidiazuron at concentrations of 0, 0.5, and 1 ppm combined with NAA at concentrations of 0, 1, 2 ppm. The data were analyzed by ANOVA at 5% level of significance, followed by BNT (Least Significant Difference) test at 5% level of significance. The results showed that the interaction between the administration of thidiazuron and NAA on the response of vanilla (Vanilla planifolia Andrews) nodule explants in vitro had a significant effect on the variables of shoot length and root length. In conclusion, the combined treatment of thidiazuron concentration 0.5 ppm and NAA concentration 1 ppm (T1N1) showed the best trend.
Co-Authors Ardyansah, Muhammad Ikrom Dwi Erwin Kusbianto Rosyady, Muhammad Ghufron