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All Journal Jurnal Agrotek UMMat BIO-EDU: Jurnal Pendidikan Biologi
Hasbi Mubarok Suud, Hasbi Mubarok
Program Studi Teknik Mesin Pertanian dan Pangan, Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Education Social Sciences

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Pengaruh pemberian thidiazuron dan NAA pada media MS terhadap regenerasi eksplan nodal vanili (Vanilla Planifolia Andrews) secara in vitro Ardyansah, Muhammad Ikrom; Kusbianto, Dwi Erwin; Suud, Hasbi Mubarok; Rosyady, Muhammad Ghufron
Jurnal Agrotek Ummat Vol 11, No 4 (2024): Jurnal Agrotek Ummat
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jau.v11i4.26325

Abstract

Vanilla is a commodity with high economic value that belongs to the Orichidaceae family. One of the problems in vanilla cultivation is unhealthy seedlings and mother plants due to Fusarium oxysporum infection. In vitro plant propagation is an alternative because it uses a small part of the plant on sterile media so that it becomes a healthy plant. The objective of this study was to determine the effect of thidiazuron and NAA on the growth response of vanilla (Vanillaplanifolia Andrews) node explants in vitro. This research method uses RAL, which consists of two factors. Thidiazuron at concentrations of 0, 0.5, and 1 ppm combined with NAA at concentrations of 0, 1, 2 ppm. The data were analyzed by ANOVA at 5% level of significance, followed by BNT (Least Significant Difference) test at 5% level of significance. The results showed that the interaction between the administration of thidiazuron and NAA on the response of vanilla (Vanilla planifolia Andrews) nodule explants in vitro had a significant effect on the variables of shoot length and root length. In conclusion, the combined treatment of thidiazuron concentration 0.5 ppm and NAA concentration 1 ppm (T1N1) showed the best trend.
The Effect of WPM (Woody Potent Medium) Dosage on the In Vitro Development of Milo Pace Variety Robusta Coffee Leaf Explants: Pengaruh Dosis Media WPM (Woody Pant Medium) Terhadap Perkembangan Eksplan Daun Kopi Robusta Varietas Milo Pace Secara In Vitro Az Zahra, Rana; Irsyadi, Muhammad Burhanuddin; Kusbianto, Dwi Erwin; Suud, Hasbi Mubarok; Rosyadi, Muhammad Ghufron; Wulanjari, Distian; Farisi, Oria Alit
BIO-EDU: Jurnal Pendidikan Biologi Vol. 10 No. 3 (2025): BIO-EDU: Jurnal Pendidikan Biologi - December 2025
Publisher : Jurusan Pendidikan Biologi, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/jbe.v10i3.10823

Abstract

Milo pace robusta coffee is a local coffee variety in Jember Regency that is threatened with extinction. One way to overcome this problem is by propagating it through tissue culture. The success of tissue culture is determined by the use of optimal culture media doses. Research related to the development of Milo pace coffee is still very limited. This study aims to obtain the best dose in woody plant medium media for the development of robusta coffee leaf explants of the Milo pace variety. This study used a completely randomized design (CRD) with a single factor, namely the WPM media dose (1 time, ¾ times, ½ times and MS 1 time as a control) which was repeated 3 times with 3 samples. Data were analyzed descriptively and statistically using analysis of variance, and continued with the DMRT test at a 95% confidence level. The results showed that the use of WPM media with 1 dose was the best dose with a percentage of imbibed explants of 77.77% with an average emergence at 2.53 ± 0.68 DAI and green live explants. Furthermore, the percentage of browning explants was still high at 88.89±19.24%, which appeared at 9.96±1.73 DAI. Various doses of WPM media were unable to induce callus in Milo Pace robusta coffee leaf explants.
Co-Authors Ardyansah, Muhammad Ikrom Az Zahra, Rana Dwi Erwin Kusbianto Farisi, Oria Alit Irsyadi, Muhammad Burhanuddin Rosyadi, Muhammad Ghufron Rosyady, Muhammad Ghufron Wulanjari, Distian