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All Journal Indonesian Journal of Biotechnology JURNAL PENDIDIKAN JASMANI DAN OLAHRAGA
Ngadiman N, Ngadiman
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Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Materials Science & Nanotechnology Public Health

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Succession of Actinomycetes During Composting Proccess of Dairy-Farm Waste Investigated by Culture-Dependent and Independent Approaches Faatih1, Mukhlissul; Widada, Jaka; N, Ngadiman
Indonesian Journal of Biotechnology Vol 13, No 2 (2008)
Publisher : Universitas Gadjah Mada

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Abstract

Mesophilic, thermophilic, and maturation phases were recognized in composting proccess. Temperaturechanges influence the microbial communities in compost within composting proccess. Actinomycetes account for alarger part of compost microbial population. The aim of this research was to study succession of actinomycetescommunity during composting of dairy-farm waste investigated by culture-dependent and independentapproaches.In culture-independent method, the succession of actinomycetes community was analyzed by nestedpolymerasechain reaction of ribosomal intergenic spacer (nested-PCR RISA) using spesific primer F243 and primerR23S followed by a second PCR using primers F968 and R23S. In culture-dependent method actinomycetes fromcompost were isolated on selective media, starch-nitrate medium and humic-acid + vitamins medium. DNA ofactinomycetes was extracted and amplified by repetitive sequence-based PCR (rep-PCR) using primer BOXA1R. Thebanding patterns were used to generate dendrograms by UPGMA clustering with NTSYS program. Microcosmcontaining sterile rice-straw and water which is inoculated with each actinomycetes isolates was used for examiningthe ability of each isolate in rice-straw degradation.The experiment results showed that succession of both bacteria and actinomycetes was occured withincomposting proccess of dairy-farm waste. Analysed by culture-independent method revealed that the highestcommunity of compost’s bacteria was on mesophilic, thermophilic, and maturation phases, respectively. WhereasPCR-nested RISA resulted the highest population of actinomycetes was on thermophilic, maturation, and mesophilicphases, respectively. By culture-dependent method was obtained 29 actinomycetes isolates from mesophilic phase,23 isolates from thermophilic phase, and 19 isolates from maturation phase. Genetic diversity analysis of the obtainedisolates showed the presence of phylogenetic grouping on each phase of composting proccess. This result illustratedthe occurance of succession of actinomycetes community in compost. The ability of each isolates in rice-strawdegradation was different, and SnT9 isolate was found to be a promising rice-straw degrader.Keywords: succession, actinomycetes, composting, nested-PCR RISA, rep-PCR
Distribution of Camphor Monooxygenase Genes in Soil Bacteria N, Ngadiman; Suenaga, Hikaru; Goto, Masatoshi; Furukawa, Kensuke
Indonesian Journal of Biotechnology Vol 10, No 2 (2005)
Publisher : Universitas Gadjah Mada

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In microbial degradation of camphor, the first step is oxidation by multiunit enzyme, camphormonooxygenase, encoded by cam genes (camA,B,C). Seven camphor-utilizing bacterial strains have been isolatedfrom soil at various locations. CamA,B,C genes of Pseudomonas putida strain PpG1 and strain GF2001 were used asprobes to explore their abundance in the camphor-utilizing bacteria. Southern analysis revealed that all of thecam genes of GF2001 could hybridize well to the SpeI-digested genomic DNA of strains tested, whereas PpG1 camgenes were not. This result suggested that the GF2001 type cam genes are widely distributed among the camphorutilizingstrains in the environment. Thus strain GF2001 and seven newly isolated strains share a commonevolutionary origin.Key words: Camphor monooxygenase genes, gene distribution, sail bacteria.
Diversity of Actinomycetes at Several Forest Types in Wanagama I Yogyakarta and Their Potency as a Producer of Antifungal Compound Nurjasmi, Reni; Widada, Jaka; N, Ngadiman
Indonesian Journal of Biotechnology Vol 14, No 2 (2009)
Publisher : Universitas Gadjah Mada

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Actinomycetes are bacterial groups that produce many secondary metabolites, which different biological activities, such as antifungi, antibacteria, antivirus, antitumor, etc. Actinomycetes are widely distributed in soil and their diversity is influenced by type of forest. The aim of this study is to investigate diversity of actinomycetes in several forest types of Wanagama I forest in Yogyakarta and their potency as a producer of antifungal compound. Soil samples under the forest of Tectona grandis, Swietenia macrophylla King, Bamboosa vulgaris, Melaleuca leucadendron, and Gliricidia maculata were used as sources of soil bacteria. Bacteria and actinomycetes communities were analyzed through culture-independent approach by RISA and nested-PCR RISA using actinomycetes spesific primer (F243), respectively. Through culture-dependent approach, isolated actinomycetes diversity were analyzed by identification of morphology (colony and cell), genetic (BOX element by rep-PCR), and secondary metabolites (thin layer chromatography). In addition, isolates were assayed for their antifungal activity against Saccharomyces cerevisae, Candida albicans, Fusarium oxysporum and Aspergillus flavus. The presence of Polyketide Synthase-I (PKS-I) and NonRibosomal Peptide Synthetase (NRPS) genes were amplified by PCR to study their correlation with antifungal activity of the actinomycete isolates. The results showed that types of forest influence diversity of rhizobacteria especially actinomycetes. According to culture-independent approach, relatively, com-</div><div>munity of rhizobacteria from the highest were soil under the forest of B. vulgaris, G. maculata, T. grandis, S.macrophylla King, and M. leucadendron, respectively. Meanwhile, community of actinomycetes from the highest were soil under the forest of G. maculata, B. vulgaris, M. leucadendron, S. macrophylla King, and T. grandis, respec- tively. Fourty-three morphologically different isolates were found by using culture-dependent approach consisting of 17 isolates were found in soil under the forest of M. leucadedron, each of 9 isolates in G. maculata and T. grandis, 6 isolates in S. macrophylla King. and 2 isolates in B. vulgaris. More diversity of secondary metabolites were observed in soil actinomycetes under the forest of M. leucadendron. Of the 43 isolates, 100% were active against S.cerevisae, 37.20% against C. albicans, 95.30% against F. oxysporum, and 83.70% against A. flavus. Antifungal activity of actinomycete isolates did not always have correlation with the presence of PKS-I and NRPS.
Sport Development Index of Banyumas Regency N, Ngadiman; Kusuma, Indra Jati; Festiawan, Rifqi
JURNAL PENDIDIKAN JASMANI DAN OLAHRAGA Vol 4, No 2 (2019): Promote a More Active and Healthier lifestyle Through Physical Education
Publisher : Universitas Pendidikan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (748.377 KB) | DOI: 10.17509/jpjo.v4i2.19181

Abstract

The purpose of this study was to evaluate the local government’s success in its development, especially in the field of sports from the aspect of open space availability, community participation, sport resource, and community fitness level (Sport Development Index). Therefore, an evaluative research approach was employed in this study. The research employed both stratified random sampling and cluster sampling techniques. The population basic characteristics included the differences of regional progress level, gender between male and female, age ranging from children aged 7-12 year old, adolescents aged 13-17 year old, and adults aged 18-40 year old, while the basic cluster characteristics used were district and sub-district or village. The research results showed that the open space index was classified in low category (0.180), human resource index in medium category (0.551), public participation index in medium category (0.572), and physical fitness index in low category (0.488). Simultaneously, the Sport Development Index of Banyumas Regency in 2010 is classified in low category (0.487).  
Co-Authors Festiawan, Rifqi Hikaru Suenaga, Hikaru Indra Jati Kusuma JAKA WIDADA Kensuke Furukawa, Kensuke Masatoshi Goto, Masatoshi Mukhlissul Faatih1, Mukhlissul Reni Nurjasmi, Reni