Garuda - Garba Rujukan Digital

Effects of Light Quality on Vegetative Growth and Flower Initiation in Phalaenopsis Dewi, Kumala; Purwestri, Yekti Asih; Astuti, Yohana Theresia Maria; Natasaputra, Lila; P, Parmi
Indonesian Journal of Biotechnology Vol 19, No 1 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (500.288 KB)

The effects of LEDs (Light-Emitting Diodes) emitting different colours namely red, blue, red andblue, and white lights on vegetative growth and fl ower initiation of Phalaenopsis have been evaluated.Phalaenopsisâotohine/taisuco fi re birdâ seedlings in vitro were subjected to different light qualities for either2 or 4 weeks, and then each seedling was planted in a plastic pot containing sphagnum and grown in thegrowth chamber under similar light quality for 3 months. For fl ower induction, mature Phalaenopsis plantshaving 4 â 6 leaves were grown for 3 months in the growth chamber under different light qualities. The leafspan, chlorophyll, gibberellin and cytokinin content were determined. In addition, the expressions of FT-likegene in the leaf, axillary bud, fl ower bud and stalk were examined.Vegetative growth was enhanced under blue, red-blue or white LEDs compared to that of the control.Gibberellin and cytokinin content increased in the seedlings subjected to white LEDs. Based on the averageof leaf span increment it was suggested that the growth of Phalaenopsis seedlings can be promoted by givingeither blue, red-blue or white LEDs. From the second experiment, it was found that fl ower induction inPhalaenopsis can be obtained in plants that had just fi nished fl owering without the application of LEDs. Theexpression of FT-like gene in the leaf as well as fl ower bud and stalk suggests that this gene is involved infl ower regulation of Phalaenopsis.

Pengaruh Pemberian BAP dan NAA terhadap Pertumbuhan Krisan (Chrysanthemum morifolium, Ram.) dalam Kultur Jaringan Astuti, Yohana Theresia Maria; Andayani, Neny
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 1 (2005): February 2005
Publisher : Universitas Atma Jaya Yogyakarta

The objective of this study was to evaluate the effect of combination benzyl amino purine(BAP) and naphtalene acetid acid (NAA) on tissue culture of Chrysanthemum. Theexperiment was conducted at The Tissue Culture Laboratory, Agriculture Faculty,Stiper Agriculture Institute. The Completely Randomized Design was applied in thisexperiment, consisting of two factors; those were BAP and NAA application. Each factorconsisted of four treatments. Each combination of treatment was carried out with ninereplications. The conclusion from this study were: Application of higher BAP and NAAconcentration increased budding of explant, whereas application of higher NAA thanBAP concentration increased the growth of bud and leaf number, also increased rootingof explant.

Upaya Mempercepat Kematangan Buah Kopi Arabika dengan Aplikasi Hormon Silalahi, Edo Hasiholan; Astuti, Yohana Theresia Maria; Kautsar, Valensi
AGROISTA : Jurnal Agroteknologi Vol. 8 No. 1 (2024): MEI
Publisher : Program Studi Agroteknologi INSTIPER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55180/agi.v8i1.982

Coffee is a plantation crop with significant economic value, commonly processed into flavorful beverages. Arabica coffee farmers generally face challenges during harvest and post-harvest, particularly with the necessity to pick ripe, red berries. However, during harvest, farmers often do not selectively pick the cherries, resulting in the collection of not only red but also yellowish or green berries. Plant growth regulators (PGRs) are synthetic chemicals that mimic the role of endogenous hormones. This study aims to investigate the interaction between different hormone types and varieties on the ripening of Arabica coffee berries, assess the impact of various hormones on berry maturity, and evaluate the effect of different varieties on the ripening process. The research utilized a completely randomized design (CRD) with two factors: plant growth regulators (PGRs) and coffee varieties. The observed parameters included the number of fruiting nodes per tree, the number of fruits per node, the number of red fruits in the first harvest, the number of red fruits in each subsequent harvest (across three stages), and the weight of green beans per 100 seeds. The analysis revealed no significant interaction between the application of different types of growth regulators (PGRs) and coffee varieties in accelerating the maturation of Arabica coffee berries. Notably, the Yellow Catura variety treated with the growth regulator Ethrel showed a better effect in accelerating berry maturity.

In silico study of RKD4 gene function in Coffea arabica L. and various cultivated plants related to embryo formation initiation Arimarsetiowati, Rina; Semiarti, Endang; Daryono, Budi Setiadi; Astuti, Yohana Theresia Maria; Prastowo, Erwin
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol. 40 No. 2 (2024)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v40i2.600

Arabica coffee supplies 60% of world coffee production because has a unique taste as superior quality beverage. Arabica coffee micropropagation can be conducted by somatic embryogenesis technique which produce clonal, fast dan uniform plant. The somatic embryogenesis (SE) process describes the integration of endogenous signals and gene reprogramming, which releases signals to initiate embryogenic processes. The use of endogenous auxin, either alone or in combination with other PGRs or stress, induces differential gene expression, which modifies the genetic program of somatic cells and regulates the transition to each stage during SE development. The RKD4 gene (RWP-RK DOMAIN-4) is a gene that plays a role in early initiation embryo formation and development. The characterization of RKD4 genes in C. arabica is still limited and under explored. The objective of this research is to explore the characteristics of RKD4 gene by comparing the difference and similarity of RKD4 gene in C. arabica and other cultivated plants. The method was initiate by identifying nucleotide sequences from the National Center for Biotechnology Information (NCBI) database. Furthermore, consists of analysis of nucletide alignment, alignment of amino acid sequences, protein analysis, protein motif functions discovery, analysis of phylogenetic tree, protein 2D and 3D-modelling and physiochemical properties. According to the analysis, there were 100 polymorphism points with a total number of mutations of 211 points. The phylogenetic tree show C. arabica L. has a very close relationship with grapes (Vitis vinivera) based on the RKD4 protein, gene structures and protein motifs. There are nine highly conserved motifs found in the protein alignment. C. arabica L. had more methyl jasmonate element responses than A. thaliana. The findings are useful to understand the intitiation of embryo formation mechanisms of C. arabica L and other cultivate plants during propagation through somatic embryogenesis in the long run.

The chemical content of Arabica coffee beans with the application of ethrell, NAA, and gibberellin to accelerate ripening in several varieties Astuti, Yohana Theresia Maria; Dharmawati, Nuraeni Dwi; Santosa, Tri Nugraha Budi; Silalahi, Edo Hasiholan; Alfandi, Iqbal; Arifin, Zaenal

Publisher : Fakultas Pertanian Universitas Yudharta Pasuruan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35891/agx.v15i2.4503

Introduction: Caffeine, antioxidants, and vitamin C are important compounds found in coffee beans. These chemical compounds affect the taste and aroma of coffee. The research aims to examine the effect of hormone application on the chemical content of coffee beans. Methods: The research was carried out at the people's coffee plantation in Kwadungan Gunung, Kledung, Temanggung, Central Java. The research uses an experimental method with two factors. Factor I variety consists of two varieties, namely Sigararutang and Yellow Catura. Factor II of the Plant Growth Regulators (PGR) application consists of four levels, namely: Control, ethrel, NAA, and gibberellin. Each treatment combination with 5 replications. The parameters observed were the number of fruit/ tree, the number of ripe fruit/ tree at the first harvest, caffeine content, vitamin C, antioxidant activity, sugar, and protein. Data were analyzed using ANOVA with the DMRT further test. Results: The number of coffee cherries of the Yellow Catura was better than the Sigarar Utang. The caffeine is better in Sigararutang in all PGR applications compared to other combinations. The antioxidant activity of Yellow Catura with the application of NAA and GA3 is better than other combinations. Vitamin C in Yellow Catura with GA3 application and control is better than other combinations. The protein in Yellow Catura with NAA application is better than other combinations. The sugar in Yellow Catura with all applications of PGR is better than the other combinations. Conclusion: PGR application and differences in varieties affect the chemical content namely caffeine, antioxidant activity, vitamin C, protein, and total sugar.

Analisis Beberapa Faktor yang Mempengaruhi Produksi Crude Palm Oil di Pabrik Kelapa Sawit PT SH Kalimantan Tengah Sebayang, Denny Swadaya Putra; Ruswanto, Adi; Astuti, Yohana Theresia Maria
Jurnal Agro Industri Perkebunan Vol. 13 No. 1 (2025)
Publisher : Politeknik Negeri Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jaip.v13i1.3896

The palm oil mill of PT SH located in Lamandau district, Central Kalimantan province, with an actual capacity of 50 tons of oil palm fruit bunches per hour, experiences problems such as the availability of fresh fruit bunch supply that is not optimal according to the factory capacity, the processing process is not smooth. There is often downtime due to damage to the machine unit during factory operation. This study aims to examine (1) the effect of fresh fruit bunches (amount or tonnage processed), (2) the effect of processing capacity (mill throughput), (3) the effect of downtime, and (4) the effect of processing costs on crude palm oil (CPO) production at the palm oil mill of PT SH by focusing on crude palm oil production as the dependent variable, then the number of fresh fruit bunches (FFB) processed, processing capacity, downtime, and processing costs as independent variables. The method used is multiple linear regression analysis modified from the Cobb-Douglas equation. The results of this study show that the factors of the number of FFB processed and processing capacity have a significant effect on CPO production. In contrast, downtime and processing costs do not significantly affect CPO production.

Establishment of an efficient primary callus induction for embryogenic potential of Coffea arabica Arimarsetiowati, Rina; Daryono, Budi Setiadi; Astuti, Yohana Theresia Maria; Semiarti, Endang
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol. 38 No. 2 (2022)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v38i2.510

Coffea arabica L. is a species of coffee that contribute for more than seventy percent of world coffee production. Various attempts have been made to obtain large quantities of planting material through propagation in vitro somatic embryogenesis technology. The objective of this experiment was to evaluate the effect of different plant growth regulators (PGRs) on callus induction (indirect somatic embryogenesis) in AS2K clone of Arabica coffee. Mother plants of Arabica coffee were established in coffee experimental field of Indonesian Coffee and Cocoa Research Institute at Andung Sari, Bondowoso, East Java, Indonesia (-7Ëš55'' ' S, 113Ëš41'' ' E) at an altitude of 1380,1 m dpl. Leaf explants were cultured on a half-strength Murashige and Skoog (MS) medium supplemented with various concentration (1.0, 2.0, 3.0 mg/L) of 2,4-D and (1.0, 2.0, 3.0 mg/L) thidiazuron in combination with 1.0 mg/L BAP. All the experiments were organized in completely random design (CDR) and repeated three times, each using minimum seven replicates (a total of 21 explants per treatment). The morphologycal and histological analysis of the different types of callus were observed. The percentage of callus formation was recorded every two weeks until eight weeks. The highest percentage of callus formation (almost 60%) was in medium containing 1 mg/L 2,4-D dan 1 mg/L BAP. Morphological and histological studies prove that the callus has a friable and embryogenic texture and begins to develop various stages of somatic embryo formation, starting with the globular, heart, torpedo and cotyledonary phases.

Pengaruh aplikasi plant growth promoting rhizobacteria (PGPR) terhadap cherelle wilt dan kualitas hasil kakao: Effect of plant growth promoting rhizobacteria (PGPR) application on Cherelle wilt and cocoa yield quality Astuti, Yohana Theresia Maria
AGROMIX Vol 13 No 2 (2022)
Publisher : Fakultas Pertanian Universitas Yudharta Pasuruan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35891/agx.v13i2.2998

This study aims to reduce cherelle wilt with plant growth promoting rhizobacteria (PGPR) application. The study was conducted in July â€“ December 2021. The study used an experimental method with a completely randomized design. Factor I PGPR applications consist of 3 kinds namely control, EM4 and PGPR. Factor II varietas consist of 2 kinds namely Sul 01 and MCC 01. The results showed that there was no significant interaction between the application of biofertilizer and cocoa varieties. The number of cherelle wilt in the application of PGPR and EM4 was lower that that the control, on the other hand the number of new pods/plant calculated the beginning until 4 months after application of PGPR and EM4 was higher than the control. The weight of 100 cocoa fermented beans in PGPR application was better than EM4 and control. The content of N and P available in the soil in the application of biofertilizer was higher than the control. The number of cherelle wilt and new pods were the same in the different varieties. The conclusion was the application of PGPR decreased the number of cherelle wilt of cocoa, increased the number of new pod, and increased the weight of 100 cocoa fermented bean. The application of PGPR and EM4 increased the available N dan P content in the soil. The difference of variety did not affect on cherelle wilt, but the quality of cocoa beans in MCC 01 was greater than Sul 01.

The chemical content of Arabica coffee beans with the application of ethrell, NAA, and gibberellin to accelerate ripening in several varieties Astuti, Yohana Theresia Maria; Dharmawati, Nuraeni Dwi; Santosa, Tri Nugraha Budi; Silalahi, Edo Hasiholan; Alfandi, Iqbal; Arifin, Zaenal
AGROMIX Vol 15 No 2 (2024)
Publisher : Fakultas Pertanian Universitas Yudharta Pasuruan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35891/agx.v15i2.4503

Introduction: Caffeine, antioxidants, and vitamin C are important compounds found in coffee beans. These chemical compounds affect the taste and aroma of coffee. The research aims to examine the effect of hormone application on the chemical content of coffee beans. Methods: The research was carried out at the people's coffee plantation in Kwadungan Gunung, Kledung, Temanggung, Central Java. The research uses an experimental method with two factors. Factor I variety consists of two varieties, namely Sigararutang and Yellow Catura. Factor II of the Plant Growth Regulators (PGR) application consists of four levels, namely: Control, ethrel, NAA, and gibberellin. Each treatment combination with 5 replications. The parameters observed were the number of fruit/ tree, the number of ripe fruit/ tree at the first harvest, caffeine content, vitamin C, antioxidant activity, sugar, and protein. Data were analyzed using ANOVA with the DMRT further test. Results: The number of coffee cherries of the Yellow Catura was better than the Sigarar Utang. The caffeine is better in Sigararutang in all PGR applications compared to other combinations. The antioxidant activity of Yellow Catura with the application of NAA and GA3 is better than other combinations. Vitamin C in Yellow Catura with GA3 application and control is better than other combinations. The protein in Yellow Catura with NAA application is better than other combinations. The sugar in Yellow Catura with all applications of PGR is better than the other combinations. Conclusion: PGR application and differences in varieties affect the chemical content namely caffeine, antioxidant activity, vitamin C, protein, and total sugar.

Title

Found 9 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 9 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 9 Documents
Search