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All Journal Program Kreativitas Mahasiswa - Gagasan Tertulis Indonesian Journal of Biotechnology Journal of Biotechnology and Natural Science ASPIRATOR - Journal of Vector-Borne Diseases Studies
Soenarwan Hery Poerwanto, Soenarwan Hery
Jurusan Biologi, Fakultas Biologi, Institut Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Other

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Cloning and Sequencing cDNA Encoding for Rhoptry-2 Toxoplasma Gondii Tachyzoite Local Isolate Artama, Wayan T.; Sari, Yulia; Subekti, Didik Tulus; Poerwanto, Soenarwan Hery; Subandono, Jarot
Indonesian Journal of Biotechnology Vol 10, No 2 (2005)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (338.765 KB)

Abstract

Rhoptry protein belongs to an excretory and secretory antigens (ESAs) that play an important role during activepenetration of parasite into the cell target. This protein an able Toxoplasma gondii to actively penetrate targetedcell, meanwhile ESAs protein stimulates intracellular vacuole modification. It is, therefore, after the parasitesuccessfully enter the cell target then Granule (GRA) proteins are responsible for the formation of parasitophorusvacuole, which is protect the fusion with other intracellular compartments such as lysosomal vacuole. Consequently,this parasite is being able to survive and multiply at the cell target. The current study was aimed to clone andsequens cDNA encoding for ROP-2 of local isolated T. gondii tachizoite through DNA recombinant technique.Total ribonucleic acid (RNA) was isolated from tachyzoites of local isolated T. gondii that were grown up in Balb/c mice. Messenger RNA was isolated from total RNA using PolyAtract mRNA Isolation System. Messenger RNA wasused as a template for synthesis cDNA using Riboclone cDNA Synthesis System AMV-RT. EcoRI adaptor fromRiboclone EcoRI Adaptor Ligation System was added to Complementary DNA and than ligated to pUC19. Recombinantplasmid was transformed into E. coli (XL1-Blue). The transformed E. coli XL-1 Blue were plated on LB agarcontaining X-Gal, IPTG and ampicillin. Recombinant clones (white colony) were picked up and grown up in theLB medium at 37oC overnight. Expression of recombinant protein was analysed by immunoblotting in order toidentify cDNA recombinant wich is express ESA of T. gondii local isolate. Recombinant plasmid were isolatedusing alkalilysis method and were elektroforated in 1% agarose gel. The isolated DNA recombinant plasmid wascut using Eco RI and then sequenced through Big Dye Terminator Mix AB1 377A Sequencer using M13 Forward andM13 Reverse primers. The conclusion of this results showed that the recombinant clone was coding for excretoryand secretory protein which has molecular weight of 54 kDa. The DNA alignments of sequence from the clonedgene showed 97% homology with gene encoding for ROP-2 of T. gondii RH isolate.Keywords: Toxoplasma gondii, tachizoite, ESA, complementary DNA, ROP2
NYAMUK SEBAGAI VACCINE: SEBUAH PEMIKIRAN FUTURISTIK Kurniawati, Milla Fajar; Agustia, Fernando Dwi; Khairiskam, Muhammad; Poerwanto, Soenarwan Hery
Program Kreativitas Mahasiswa - Gagasan Tertulis PKM-GT-2014
Publisher : Ditlitabmas, Ditjen DIKTI, Kemdikbud RI

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Abstract

The development of recombinant DNA biotechnology especially lately has opened new expectations. This technology allows to produce a vaccine that has yet to be made. In addition, this technology can also be used to improve existing vaccines, so we get more vaccine is safe and effective. This technological innovation allows the manufacture of vaccines and subunit vaccines idiotipe (therapeutic vaccines). Therefore, we initiated the concept of mosquitoes as Vaccine Delivery Host: A Futuristic Thought as the protection of effective prevention and control as a solution to the crisis facing outbreaks of infectious and parasitic diseases that occur in Indonesia. This concept utilizes a mosquito vector for the production of antigens by recombinant DNA techniques. Where specific antigens of microbial parasites were cloned in bacterial plasmids engineered to increase expression of the inserted gene in the cells of the mosquito vector, so that when the mosquito bites do not transmit disease, but actually increase the immune response to a disease. The aim of this concept is in addition to the protection and control of outbreaks of infectious diseases and parasitic diseases in endemic areas, it is also said in response to the health crisis in contemporary conditions.Keywords: mosquito vector, recombinant DNA, vaccine
Ethnopharmacological Study of Medicinal Plants in Kedungpoh Village, Nglipar Sub-district, Gunungkidul District, Special Region of Yogyakarta Untari, Ludmilla Fitri; Vidhantyka, K.H. Mahadhevy Ryrathna; Ananmaimuna, Jovika Syifa; Nilamsari, Regina; Malika, Pradhika Cikal; Poerwanto, Soenarwan Hery; Siswanti, Dwi Umi; Purwanto, Hari
Journal of Biotechnology and Natural Science Vol. 5 No. 1 (2025): June
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jbns.v5i1.12972

Abstract

The scientific study of ethnopharmacology investigates the medicinal uses of plants by different ethnic groups and plays a crucial role in the early-stage drug discovery process by identifying potential drug candidates from traditional remedies. The transmission of knowledge concerning the diversity of plant species and their utilization is typically an intergenerational process, facilitating the preservation of cultural heritage and the transfer of experiential knowledge. The present study was conducted in the village of Kedungpoh, Nglipar, Gunungkidul. The objective of this study is to investigate the utilisation, management and comprehension of medicinal plants within the Kedungpoh village in Gunungkidul.The research methodology comprised of observational studies and interviews with local residents. The results revealed the utilization of 24 plant species, which classified into 17 distinct families by the local community. The utilization of these plants encompassed various plant organs, including leaves, stems, roots, fruits, and rhizomes, which were employed in different processing methods, such as crushing, boiling, or rubed. The most prevalent and utilized plant families were Asteraceae, which are notable for their abundance of bioactive compounds, including flavonoids, alkaloids, and essential oils, which have been demonstrated to be efficacious in addressing a wide range of health concerns. This study provides substantial support for scientific laboratory research into the safety, quality and efficacy of traditional medicine practices, with a view to further utilization of traditional medicine, whilst also protecting the intellectual property rights of traditional knowledge holders.
Karakteristik Habitat Larva Nyamuk dan Kepadatan Nyamuk Dewasa (Diptera: Culicidae) di Kabupaten Jembrana, Provinsi Bali (Analisis Data Sekunder Rikhus Vektora 2017): Habitat Characteristics of Mosquito Larvae and Density of Adult Mosquitoes (Diptera: Culicidae) in Jembrana Regency, Bali Province (Rikhus Vektora 2017 Secondary Data Analysis) Wahono, Tri; Widjayanto, Dionisius; Poerwanto, Soenarwan Hery
Aspirator Vol 14 No 1 (2022): Jurnal Aspirator Volume 14 Nomor 1 2022
Publisher : Perkumpulan Entomologi Kesehatan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/asp.v14i1.5038

Abstract

Abstract. Mosquitoes play a direct role in the spread of various vector-borne diseases in tropical countries. The three main mosquito genera that spread disease in Indonesia are Aedes, Culex, and Anopheles. Jembrana Regency, Bali Province is an endemic area for dengue, malaria, and filariasis. Jembrana Regency is still reporting cases of DHF, malaria and is in the stage of administering filariasis mass prevention drugs in 2020. Control of mosquito vectors is influenced by various things, such as the specific habitat of the larvae and the density of adult mosquitoes. The study used data from Rikhus Vektora 2017 in Jembrana Regency, Bali Province by characterizing specific habitats, measuring water parameters, calculating mosquito density with the Man Hour Density formula, and measuring environmental parameters in all types of ecosystems. Specific habitat of Aedes sp. in the form of a puddle of freshwater that is not in contact with the ground, Culex sp. in the form of a puddle of fresh water in contact with the ground, and Anopheles sp. a pool of fresh-brackish water in contact with the ground with different water parameters but still in a close range. The mosquito density was dominated by Culex sp. in all ecosystems except for coastal ecosystems close to settlements dominated by Aedes sp. All adult mosquitoes (Aedes sp., Culex sp., and Anopheles sp.) in Jembrana Regency are more zoophilic. The behavior of all adult mosquitoes is different in each ecosystem, which can be endophagic or exophagic. Environmental parameters in Jembrana Regency have the potential to support mosquito breeding. Abstrak. Nyamuk berperan langsung dalam penyebaran berbagai penyakit tular vektor di negara tropis. Tiga genus nyamuk utama penyebaran penyakit di Indonesia adalah Aedes, Culex, dan Anopheles. Kabupaten Jembrana, Provinsi Bali merupakan daerah endemis untuk DBD, malaria maupun filariasis. Kabupaten Jembrana masih melaporkan kasus DBD dan malaria serta dalam tahap pemberian obat pencegahan massal filariasis pada tahun 2020. Pengendalian vektor nyamuk dipengaruhi berbagai hal seperti karakteristik habitat larvanya dan kepadatan nyamuk dewasa. Penelitian menggunakan data Rikhus Vektora 2017 di Kabupaten Jembrana, Provinsi Bali dengan melihat karakteristik habitat larva, pengukuran parameter air, dan kepadatan nyamuk dengan perhitungan Man Hour Density, serta pengukuran parameter lingkungan pada semua tipe ekosistem. Habitat larva Aedes sp. berupa genangan air tawar yang tidak bersentuhan dengan tanah; Culex sp. berupa genangan air tawar bersentuhan dengan tanah; dan Anopheles sp. genangan air tawar-payau yang bersentuhan dengan tanah dengan parameter air yang berbeda, namun masih dalamrentang yang tidak jauh. Kepadatan nyamuk didominasi oleh Culex sp. di semua ekosistem kecuali pada ekosistem pantai dekat dengan pemukiman didominasi oleh Aedes sp. Semua nyamuk dewasa (Aedes sp., Culex sp., dan Anopheles sp.) di Kabupaten Jembrana lebih bersifat bersifat zoofilik. Perilaku semua nyamuk dewasa berbeda di setiap ekosistem, dapat bersifat endofagik maupun eksofagik. Parameter lingkungan di Kabupaten Jembrana memiliki potensi untuk mendukung perkembangbiakan nyamuk.
Co-Authors Ananmaimuna, Jovika Syifa Didik Tulus Subekti Dwi Umi Siswanti Fernando Dwi Agustia, Fernando Dwi Hari Purwanto Jarot Subandono Malika, Pradhika Cikal Milla Fajar Kurniawati, Milla Fajar Muhammad Khairiskam, Muhammad Nilamsari, Regina Tamaulina Br Sembiring Tri Wahono Untari, Ludmilla Fitri Vidhantyka, K.H. Mahadhevy Ryrathna Wayan T. Artama Widjayanto, Dionisius