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All Journal Majalah Kedokteran Bandung Nexus Kedokteran Klinik Nexus Biomedika Nexus Kedokteran Translasional Indonesian Journal of Biotechnology Indonesian Journal of Medicine
Jarot Subandono
Biochemistry Laboratory, Medical Faculty of Sebelas Maret University, Surakarta.
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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Hubungan Faktor Sosial Ekonomi dengan Kejadian Prolapsus Uteri di RSUD Dr. Moewardi Putra, Ivan Aristo Suprapto; Darto, .; Subandono, Jarot
Nexus Kedokteran Klinik Vol 2, No 3 (2013): Nexus Kedokteran Klinik
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Background: Socioeconomic factors in Indonesian citizen was different on every person. Especially on work, many variants of works would cause on variants of workload. On the other side, work varied effect on different income. Earnings would affect on the food consumed. Low collagen contained food intake could affect the low level of collagen. Risk factors for uterine prolapse was higher workload, higher parity and lower collagen level. Methods: This research was an analytic obervational with case control design. Sampling technique was fixed disease sampling for the case group and random sampling for the control group. The research was conducted by interviewing a sample directly in the  obstetric clinic  Dr. Moewardi hospital The sample was 36 person, divided into 18 samples of group cases and 18 control group samples. Dependent variable was the incidence of uterine prolapse and independent variables were socioeconomic factor. Data were analyzed by chi-square test then followed by multivariate analyse  logistic regression. Results: Distribution characteristics of uterine prolapse by age most results were in the age of 40-49 (16.67%). Based on the results, the highest number of children was 4 children (16.7%). The test results with regression analysis showed significance between socioeconomic factors to the incidence of uterine prolapse. Workload had p = 0.017 and OR = 0.186, p = 0.039 whereas income., and OR = 7.208, 95% confidence interval. Nagelke R square of 38.2%. Conclusions: Socio economic factor include workload and income significantly affected incidence of uterine prolapse. Keywords: Socio-economic factors, workload, income, uterine prolapse 
Efek Perlindungan Susu Kedelai (Glycine max) Ultra High Temperature (UHT) Terhadap Lambung Mencit Wijnaputri, Ginong Pratidina; Listyaningsih, Endang; Subandono, Jarot
Nexus Biomedika Vol 1, No 2 (2012): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: White Soybean (Glycine max) Milk Ultra High Temperature (UHT) is potential to protect gaster because it contains flavonoid. This study aimed to investigate the protective effect of white soybean milk UHT on mice’s gaster due to aspirin. Higher dose of the milk reduces the damaged on gastric mucous induced by aspirin Methods: This was a labroratory experimental research with posttest only controlled group design conducted in in Histology Laboratory of Sebelas Maret University. The samples were thirty five mice divided into 5 groups. Negative control group (KN) was given normal food and aquadest, positive control group (KP) was given aspirin dose 2,275 mg/20g Body weight (W), dan first treatment group (P1) was given cimetidine dose 0,78 mg/20g W second treatment group (P2) and third treatment group (P3) was given soybean milk UHT dose 0,7ml/20 g W and 1,4 ml/20 g W. All treatments for KN, KP, P1 , P2, P3 was given in 10 days. Aspirin was given to KP, P1 , P2, P3 with dose 2,275 mg/20g weight of mice on day 8, 9 and 10. to evaluate the  level of damage based on histologic appearance, gastric staining was performed using Haem eos. Results: Kruskal-Wallis test result showed significant result among four groups. Mann-Whitney showed significant result for KN compared to KP,P1,P2 , P3 and KP compared to P1, P2 and P3 ; while P1 compared to P2, P3 and P2 compared to P3 had no significant result. Conclusion: White Soybean (Glycine max) Milk Ultra High Temperature(UHT) protect mice’s gastric from gastritis. However, higher dose of White Soybean (Glycine max) Milk Ultra High Temperature(UHT) does not increase the protective effect of white soybean milk UHT on mice’s gaster due to aspirin. Keywords: White Soybean (Glycine max) Milk Ultra High Temperature(UHT), gastric protection, gastritis, aspirin. 
Hubungan Hiperglikemia Dengan Kejadian Stroke Hemoragik Pada Pemeriksaan Multislice CT-Scan Kepala Tanpa Kontras Di RSUD DR. Moewardi Surakarta Christyan, Fernando Feliz; Widiastuti, .; Subandono, Jarot
Nexus Kedokteran Klinik Vol 3, No 1 (2014): Nexus Kedokteran Klinik
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Background: Stroke is the third most common cause of death in the world, with a high prevalence of death in low-and middle-income countries. Hyperglycemia has been associated with atherosclerotic lession resulting in cerebral aneurysm which can lead to hemorrhagic stroke. CT-Scan is considered sufficiently sensitive for detecting acute hemorrhage, as well as detecting mass lessions. The aim of this study was to determine the correlation between hyperglycemia and hemorrhagic stroke incident on noncontrast multislice CT-Scan of the head examination. Methods: This study was an observational study with the analytical approach to the subject of cross-sectional study. 70 subjects were selected by fixed- disease sampling methods. These subjects have been performed a noncontrast multislice CT-Scan of the head examination. Data was collected by reading the result of blood glucose level and CT-Scan of the head examination on patient’s medical record. Data were processed with Statistical Product and Service Solution (SPSS) 17.00 for Windows Results: Chi-Square models showed that X2 = 7,19 with 5% significancy. OR (Odds Ratio) in hemorrhagic stroke was 4,26 (CI 95% = 1,43-12,74). Patient with hyperglycemia increases the risk of getting hemorrhagic stroke  4,26 (4) times than patient without hyperglycemia. Conclusion: There is a correlation between hyperglycemia and hemorrhagic stroke incident. Keywords: hyperglycemia, hemorrhagic stroke, noncontrast head CT-Scan  
Hubungan Antara Hiperglikemi dengan Kejadian Infark Serebri pada Pemeriksaan Multi Slice CT-scan Kepala Tanpa Kontras di RSUD Dr. Moewardi Agustina, Siska Dewi; Widiastuti, .; Subandono, Jarot
Nexus Kedokteran Klinik Vol 3, No 1 (2014): Nexus Kedokteran Klinik
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Background: In recent year, numerous studies reported that stress hyperglycemia has been shown to be a risk factor for neurocardiovascular disease, with high  risk of dying from coronary disease such as stroke.  Stroke is the third leading cause of death in the world, with a high prevalence of death in low-and middle-income countries. Hyperglycemia has been associated with increased cerebral lactate resulting in local brain tissue acidosis. It worsens mitochondrial function in the penumbra, the moderately ischemic tissue of the brain. CT-scans are considered sufficiently sensitive for detecting mass lesions, such as a brain mass or abscess, as well as detecting acute hemorrhage.  The aim of this study is to determine the relationship between hyperglycemia with cerebral infarction in non contrast  multi slice head CT-scan. Methods: This was an observational cross sectional study. A total of 70 subjects were selected by fixed disease sampling, they were patients who have been perfomed non contrast multi slice head CT-scan in Dr. Moewardi Hospital. Data were collected from the report result of non contrast multi slice head CT-scan and the result of glucose blood level on patiens’medical record.Data analysis used chi square models and were processed with Statistical Product and Service Solutions (SPSS) 17.00 for Windows. Results: This study showed a positive association and statistically significant between hyperglycemia with cerebral infarction (χ2 = 8,123; p<0.05) with moderate correlation strength. The result of OR (Odds Ratio) for cerebral infarction is 4,667 (CI 95% = 1,557 – 13.983). Confounding factor, e.g blood pressure and age  have been controlled. Conclusions: There is a significance association between hyperglycemia with cerebral infarction in multi slice head CT-scan noncontrast at Dr Moewardi Hospital . Keywords: Hyperglycemia, cerebral infarction, head CT-scan without contrast 
Efek Hepatoprotektor Ekstrak Daun Dandang Gendis (Clinacanthus nutans) terhadap Kadar SGPT Tikus Putih (Rattus novergicus) yang Diinduksi Parasetamol Sasongko, Devi Purnamasari; Subandono, Jarot; ., Martini
Nexus Kedokteran Translasional Vol 1, No 2 (2012): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Background: Paracetamol was a safe drug, but would cause oxidative stress if  taken too much.. Dandang gendis leaves contained a flavonoid antioxidant. This research intended to prove the antioxidant effect of dandang gendis leaves that could prevent liver cell damage of white rat induced by paracetamol. Methods: This research was an experimental laboratoric with post test only control group design. This research had taken place at Parasitology and Micology Faculty of Medicine Sebelas Maret University Surakarta. The sample was 32  Wistar white rats. The dependent variable was the SGPT level of white rats and the independent variable was the dandang gendis leaves extract. The white rats were divided into 4 groups: negative control group (KK0), positive control group (KK1), first threated group (KP1), and second threated group (KP2). KP1 had been given 30 mg/200 gr BB dose and KP2 had been given 60 mg/200 gr BB dose for 14 days. At 11th –  13th days, the white rats from KK1, KP1, and KP2 had been given 291.6 mg/200 gr BB dose of paracetamol. At 14th day, rat’s blood had been taken from orbitalis sinus. The damage of the liver cell had been measured with SGPT laboratory test. The data had been analyzed with one way ANOVA test then with post hoc test (α = 0.05). Results: The highest rate of SGPT levels was KK1, following KP1, KP2, and the lowest was KK0. Oneway ANOVA test resuls showed a significant difference among the four groups with p = 0.000. Post hoc test results showed a significant difference between KK0 – KK1 (p = 0.003) and KK0 – KP2 (p = 0.019) whereas between KK0 – KP1 (p = 0.204), KK1 – KP1 (p = 0.885), KK1 – KP2 (p = 0.077), and KP1 – KP2 (p = 0.932) had no significant difference. Conclusion: Giving dandang gendis leaves extract was not  significant to raise the SGPT level of white rat induced by paracetamol. Raising dandang gendis leaves extract doses was not significant to raise its hepatoprotector effect. Keywords: dandang gendis leaf, SGPT, white rat, hepatoprotector, paracetamol
Pengaruh Pemberian Ekstrak Daun Sambiloto Terhadap Kadar Kolesterol LDL Darah Tikus Putih (Rattus norvegicus) Anggraeni, Yohana Trissya; Subandono, Jarot; Kustiwinarni, .
Nexus Biomedika Vol 2, No 3 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: Andrographis paniculata,Nes leaves contain flavonoids, beta-sitosterol, and andrographolide which can lower LDL cholesterol level of blood. The objective of this research was to determine the effect of Andrographis paniculata,Ness leaves extract against blood LDL cholesterol level of white rats (Rattus norvegicus). Methods: This study was a laboratory experimental research design, using posttest only controlled group design. Subjects of this research were white rats (Rattus norvegicus), strain Wistar, male, 3 months old, 200 grams weight. By incidental sampling technique, data were collected from 25 rats. Those rats were arranged into 5 groups, each group consisted of 5 rats. The normal control group (KKn) was administered distilled water, while the negative control group (KKn(-)) and the 1st (KP1), 2nd (KP2) and 3rd treatment group (KP3) were administered high cholesterol diet (6.25 g/kg BW/day of egg yolk). Besides that, the KP1, KP2 and KP3 were treated with 160 mg, 320 mg, and 640 mg per 200 g BW of Andrographis paniculata,Ness leaves extract, successively. This research was held for 28 days. Data of blood LDL cholesterol levels were collected 10-12 hours after the last treatment. Later, the data was analyzed by One-Way ANOVA test (α = 0.05) and Posthoc Multiple Comparisons (LSD) test (α = 0.05). Results: The one-way ANOVA test showed a significant difference among five groups p = 0.000 (p <0.05). Whereas, the LSD test showed significant differences between KKn-KKn(-), KKn-KP1, KKn-KP2, KKn-KP3, KKn(-)-KP1, KKn(-)-KP2, KKn(-)-KP3, (p < 0.05) and non-significant differences between KP1-KP2, KP1-KP3, and KP2-KP3 (p > 0.05). Conclusions Andrographis paniculata,Ness leaves extract significantly lowered blood LDL cholesterol levels of Rattus norvegicus but the increase of the dose did not enhance  its function in lowering blood LDL cholesterol. Keywords: Andrographis paniculata Ness leaves extract, LDL cholesterol 
Cloning of cDNA Encoding GRA1 Protein of Tachyzoite Toxoplasma Gondii Local Isolate Sulistyaningsih, Erma; Moeljopawiro, Sukarti; Subandono, Jarot; Artama, Wayan T.
Indonesian Journal of Biotechnology Vol 10, No 1 (2005)
Publisher : Universitas Gadjah Mada

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Gene encoding GRA1 protein is potent DNA-vaccine candidate against toxoplasmosis. The aim of the researchwas to clone the gene encoding GRA1 protein of tachyzoite Toxoplasma gondii local isolate by DNA recombinanttechnology. Tachyzoite was grown in Balb/c mice in vivo. Messenger RNA was isolated from total RNA and itwas used to synthesis cDNA. Complementary DNA encoding GRA1 protein of tachyzoite Toxoplasma gondii localisolate was amplified and cloned in a prokaryote cloning vector. The recombinant GRA1-encoding gene was thendigesting using EcoRI restriction endonuclease and sequencing. The result showed that the recombinant GRA1-encoding gene consisted of DNA sequences encoding all signal peptide and mature peptide of GRA1 protein.Alignment of recombinant GRA1 sequence to gene encoding GRA1 protein of Toxoplasma gondii RH isolate showed100% homologous.Keywords: GRA1 protein, Toxoplasma gondii, tachyzoite, cloning, cDNA
Cloning and Sequencing cDNA Encoding for Rhoptry-2 Toxoplasma Gondii Tachyzoite Local Isolate Artama, Wayan T.; Sari, Yulia; Subekti, Didik Tulus; Poerwanto, Soenarwan Hery; Subandono, Jarot
Indonesian Journal of Biotechnology Vol 10, No 2 (2005)
Publisher : Universitas Gadjah Mada

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Rhoptry protein belongs to an excretory and secretory antigens (ESAs) that play an important role during activepenetration of parasite into the cell target. This protein an able Toxoplasma gondii to actively penetrate targetedcell, meanwhile ESAs protein stimulates intracellular vacuole modification. It is, therefore, after the parasitesuccessfully enter the cell target then Granule (GRA) proteins are responsible for the formation of parasitophorusvacuole, which is protect the fusion with other intracellular compartments such as lysosomal vacuole. Consequently,this parasite is being able to survive and multiply at the cell target. The current study was aimed to clone andsequens cDNA encoding for ROP-2 of local isolated T. gondii tachizoite through DNA recombinant technique.Total ribonucleic acid (RNA) was isolated from tachyzoites of local isolated T. gondii that were grown up in Balb/c mice. Messenger RNA was isolated from total RNA using PolyAtract mRNA Isolation System. Messenger RNA wasused as a template for synthesis cDNA using Riboclone cDNA Synthesis System AMV-RT. EcoRI adaptor fromRiboclone EcoRI Adaptor Ligation System was added to Complementary DNA and than ligated to pUC19. Recombinantplasmid was transformed into E. coli (XL1-Blue). The transformed E. coli XL-1 Blue were plated on LB agarcontaining X-Gal, IPTG and ampicillin. Recombinant clones (white colony) were picked up and grown up in theLB medium at 37oC overnight. Expression of recombinant protein was analysed by immunoblotting in order toidentify cDNA recombinant wich is express ESA of T. gondii local isolate. Recombinant plasmid were isolatedusing alkalilysis method and were elektroforated in 1% agarose gel. The isolated DNA recombinant plasmid wascut using Eco RI and then sequenced through Big Dye Terminator Mix AB1 377A Sequencer using M13 Forward andM13 Reverse primers. The conclusion of this results showed that the recombinant clone was coding for excretoryand secretory protein which has molecular weight of 54 kDa. The DNA alignments of sequence from the clonedgene showed 97% homology with gene encoding for ROP-2 of T. gondii RH isolate.Keywords: Toxoplasma gondii, tachizoite, ESA, complementary DNA, ROP2
Efek Kortikosteroid Dosis Rendah terhadap Kadar Soluble Receptor for Advanced Glycation End Products Mencit Balb/C Model Sepsis Prasetyo, Diding Heri; Subandono, Jarot; Aisyah, Siti
Majalah Kedokteran Bandung Vol 47, No 1 (2015)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Penggunaan kortikosteroid dosis rendah pada penatalaksanaan sepsis tahap awal masih diperdebatkan. Soluble receptor for advanced glycation end products (sRAGE) merupakan biomarker keparahan dan keluaran yang buruk pada sepsis. Penelitian ini bertujuan untuk menganalisis efek penggunaan kortikosteroid dosis rendah terhadap kadar sRAGE serum pada mencit Balb/C model sepsis tahap awal. Penelitian ini merupakan penelitian eksperimental laboratoris dengan sampel 30 ekor mencit Balb/C jantan dibagi menjadi kelompok kontrol, sepsis, dan sepsis+kortikosteroid dosis rendah. Penelitian dilakukan di laboratorium Histologi dan Biomedik Fakultas Kedokteran Universitas Sebelas Maret Surakarta periode Juni-Desember 2013. Untuk membuat model sepsis, hewan coba diinokulasi lipopolisakarisa/LPS (E. coli) dosis 0,1 mg/mencit secara intraperitoneal (i.p.). Kelompok mencit kontrol tidak diinokulasi selama penelitian. Kortikosteroid dosis rendah yang digunakan adalah metil prednisolon dosis 0,05 mg/mencit/hari secara i.p. Mencit kontrol tidak diinokulasi selama penelitian. Kadar sRAGE kelompok kontrol 54,29±16,28 pg/mL, sepsis 78,12±13,38 pg/mL, dan kortikosteroid dosis rendah 63,39±11,07 pg/mL. Kortikosteroid dosis rendah secara bermakna menurunkan kadar sRAGE (p=0,044) dibanding dengan kelompok sepsis. Simpulan, penggunaan kortikosteroid dosis rendah menurunkan kadar sRAGE pada hewan coba model sepsis tahap awal. [MKB. 2015;47(1):10–5]Kata kunci: Kortikosteroid dosis rendah, sepsis, sRAGEEffects of Low-Dose Corticosteroid on Soluble Receptor for Advanced Glycation End Products  in Balb/C Mice Sepsis Model The use of low-dose corticosteroids in the management of early sepsis is still under debate. Soluble receptor for advanced glycation end products (sRAGE) is a biomarker of severity and poor outcome of sepsis. This study aimed to analyze the effects of the use of low-dose corticosteroids on sRAGE serum levels in Balb/C mice model of early sepsis. This study was an experimental research laboratory study with 30 male Balb/C mice which divided into control, sepsis and sepsis+low-dose corticosteroids groups. The study was conducted at Histology and Biomedical Laboratory, Faculty of Medicine, Sebelas Maret University, Surakarta, from June to December 2013. Sepsis was induced in the male Balb/C mice by inoculation with an intraperitoneally (i.p.) injection of lipopoly-saccharide/LPS (E. coli) with a dose of 0.1 mg/mice/i.p.for sepsis mice model. Control mice were not inoculated during the study. Low-dose corticosteroids used was methyl prednisolone at a dose of 0.05 mg/mice/day/i.p. Levels of sRAGE  54.29±16.28 pg/mL in control group, 78.12±13.38 pg/mL in sepsis group, and 63.39±11.07 pg/mL in low-dose corticosteroids group. Low-dose corticosteroids significantly decreased sRAGE level (p=0.044) compared to the sepsis group. In conclusion, the use of low-dose corticosteroids reduces levels of sRAGE in early sepsis.   [MKB. 2015;47(1):10–5]Key words: Low-dose corticosteroids, sepsis, sRAGE DOI: 10.15395/mkb.v47n1.392   
Effect of Ethanolic Extract in Moringa oleifera, Lam. Leaf on Uric Acid Levels and Body Surface Area of Kidney Glomerulus in Wistar Rats (Rattus novergicus) Induced Metabolic Syndrome Model Darmawan, Muhammad Dzaki; Subandono, Jarot; Setyawan, Novan Adi; Budiani, Dyah Ratna
Indonesian Journal of Medicine Vol. 6 No. 4 (2021)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

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Background: There has been an increasing prevalence of metabolic syndrome (MS) caused by life style such as sedentary behavior and western diet. Metabolic syndrome causes degeneration in ren’s structure and processes the elimination of by product in metabolism, which is uric acid. Moringa oleifera Lam. leaves contain antioxidant that can repair damages caused by MS. Studies about improvement of ren’s structure and uric acid level related to Moringa oleifera Lam. leaves’ consumption has not been found yet. Therefore, this study was intended to examine the effects of ethanolic extract in Moringa oleifera Lam. leaves to uric acid and glomerular surface area in male wistar rat (Rattus norvegicus) with induced metabolic syndrome model.Method: This study is an experimental laboratory study. The subjects of this study consisted of 30 rats which were divided into 5 groups with 6 in each group. K1 is control group, K2 is MS group, and K3, K4, and K5 are MS groups given variety of ethanolic extract doses. The induction of MS was done by giving high-fat diet in 28 days and injection of streptozotocin-nicotinamide (STZ-NA) in the 25th day. Rats in group K3, K4, and K5 were given doses of 150, 250, and 350 mg/kgBW in 28 days.Results: The administration of high-fat diet for 28 days and injection of STZ-NA caused MS condition in rats.  Repeated ANOVA and One-Way Anova test showed that the administration of ethanolic extract in Moringa oleifera Lam. leaves with doses of 150, 250, and 350 mg/kgBW in 28 days decreased uric acid significantly (p= 0.001; p=0.001; p= 0.001). Another result also found that ethanolic extract from Moringa oleifera Lam. leaves with doses of 250 and 350 mg/kgBW increased area of glomerular surface area in rats significantly.Conclusion: The administration of ethanolic extract from Moringa oleifera Lam. leaves with doses of 150, 250, and 350 mg/kgBW for 28 days decreased uric acid level in rats. Ethanolic extract of Moringa oleifera Lam. leaves 250 and 350 mg/kgBw doses increase glomerular surface area.Keywords: Moringa Oleifera, uric acid, glomerular cross-sectional area, metabolic syndrome, kidneyCorrespondence: Muhammad Dzaki Darmawan. Study Program of Medical Doctor Education, Faculty of Medicine, Universitas Sebelas Maret. Email: mdddzaki@student.uns.ac.id.Indonesian Journal of Medicine (2021), 06(04): 412-422https://doi.org/10.26911/theijmed.2021.06.04.07
Co-Authors . Darto . Kustiwinarni . Widiastuti Darmawan, Muhammad Dzaki Devi Purnamasari Sasongko Didik Tulus Subekti Diding H P Diding Heri Prasetyo Dyah Ratna Budiani Endang Listyaningsih Erma Sulistyaningsih Fernando Feliz Christyan Ginong Pratidina Wijnaputri Ida Nurwati Ivan Aristo Suprapto Putra Martini . Novan Adi Setyawan Siska Dewi Agustina Siti Aisyah Soenarwan Hery Poerwanto, Soenarwan Hery Sri Hartati Hadinoto Sukarti Moeljopawiro Tamaulina Br Sembiring Wayan T. Artama Yohana Trissya Anggraeni