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All Journal BERITA BIOLOGI Jurnal Medik Veteriner Journal of Fisheries & Marine
Adam Robisalmi, Adam
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Veterinary

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ESTIMASI HERITABILITAS DAN RESPONS SELEKSI IKAN NILA HITAM (Oreochromis niloticus) DI TAMBAK Robisalmi, Adam
BERITA BIOLOGI Vol 18, No 1 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i1.3540

Abstract

Genetic improvement in the establishment of a rapid growth strain of Nile tilapia might be achieved through selective breeding program This research was aimed to estimate heritability and response selection of nile tilapia in brackishwater pond. Breeding  activity was carried out in fresh water in hapa 2x1x1 m. Males and females were breeding in pairs with a ratio of 1: 1. The number of established families was 19 families of 50 broodstock pairs. Harvesting of larvae was done on day 14. Larvae were collected and then kept in net 2 x 2 x1 m  with stocking density 125 fish/m2 for 60 days. The next stage was sexing  by grouping the male and female populations in each family. Grow out was carried out in net cage (3 x 5 x1.5 m ) that were placed in brackiswater pond at salinity 25–30 g/L with density 10 fish/m2.  Selection of broodstock in the male and female populations was done on weight of 250–300 g. Cut off selection was determined on weight from 316 to 382g (male) and 221–315 g (female). The results of this study showed that the estimation value for heritability of male and female were 0.42 ± 0.22 and 0.42 ± 0.23 and the estimation value for response selection were 10.66% (male) and 10.92% (female).   
Validation of Analytical Method for Aeromonas hydrophila Identification using Analytical Profile Index (API) 20E KIT Method Artati, Diah; Oman, Mohammad; Supriyanto, Supriyanto; Sukarta, Dede; Robisalmi, Adam
Jurnal Medik Veteriner Vol. 7 No. 1 (2024): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss1.2024.88-104

Abstract

The high demand for fish consumption has an impact on increasing aquaculture productivity and causes the vulnerability of increasing bacterial populations in aquaculture fields, so more rapid handling is needed. The use of phenotypic KIT methods (API 20E) has been applied as a targeted and efficient identification support in targeting better bacterial identification accuracy but often provides unequal results. Method validation is one of the general requirements for the competence of a laboratory evaluation: to provide coherent, interpretable, and accurate results with known uncertainties. The purpose of this study was to validate the API 20E KIT method for the identification of A. hydrophila. The conventional method used as a reference is SNI 7303.1:2015. The validation parameters consisted of determining the limit of detection, sensitivity, and specificity tests, as well as the positive predictive value and negative predictive value. The results showed that the limit of the detection value of the API 20E KIT was at a concentration of 100 CFU/mL with an ID of 99.00%. The sensitivity and specificity values in the positive and negative target samples were 100% with a positive predictive value and a negative predictive value of 0%, respectively. In conclusion, the API 20E KIT method as an alternative test method or rapid test was proven valid for identifying A. hydrophila by the test results using the reference method.
Effect of Crossbreeding on Fecundity, Growth Performance, and Heterosis of Black Tilapia, Red Tilapia, and Mozambique Tilapia Reared in Earthen Ponds in West Java, Indonesia Robisalmi, Adam; Listiyowati, Nunuk; Pamungkas, Wahyu; Artati, Diah; Mustofa, Bisri; Malini, Desak Made
Jurnal Ilmiah Perikanan dan Kelautan Vol. 17 No. 1 (2025): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.vi.55958

Abstract

Graphical Abstract   Highlight Research The crossbred performance of the four tilapia strains showed diverse heterosis values, with some being better, worse, or the same as their parents. Crossbred of black tilapia produced the best growth and fecundity performance. A hybrid of Mozambique tilapia and black tilapia showed the highest mid-parent heterosis value on growth traits but produced negative heterosis on fecundity, biomass, and survival traits. Crossbred of black tilapia and Mozambique tilapia have the potential to be used as candidates for cultivation and performance improvement through selection, although there are depressions and crossover advantages that are not prominent.     Abstract Increased tilapia production is challenged by genetic decline. Hybridization efforts for performance improvement through a selection of the best parent and strain pairs were a promising option. The objective of this study was to evaluate the crossing of black tilapia, red tilapia, and Mozambique tilapia against the performance of fecundity, growth, and survival and estimate the value of heterosis. The experimental design used a completely randomized design with 3 replications with the treatment of different populations of crosses. The rearing activities were carried out in earthen ponds for 150 days with a stocking density of 10 fish/m2. The parameters observed included egg fecundity, growth, survival, and the value of heterosis. The results showed that the fecundity and growth values of crossbred black tilapia were significantly higher than others (p<0.05). The highest survival rate was shown by crossing pure strains of red tilapia. The hybrid of Mozambique tilapia and black tilapia (♀ MJ x ♂ NW) showed the highest mid-parent heterosis value on growth characters but produced negative heterosis on characters, fecundity, biomass, and survival. Overall, the crossbred of black tilapia (♀BS x ♂ NW) performed better than the inbred strains, with positive mid-parent heterosis in all characters measured. These results indicate that crossbreeding has the potential to be used as a candidate for cultivation and performance improvement through selection, although there were depressions, and the superiority of the crosses was not prominent.
Co-Authors Artati, Diah Bisri Mustofa Desak Made Malini Listiyowati, Nunuk Oman, Mohammad Sukarta, Dede Supriyanto Supriyanto Wahyu Pamungkas