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All Journal HAYATI Journal of Biosciences METAMORFOSA Journal of Biological Sciences Jurnal Veteriner Jurnal Biologi Udayana Agrikultura Biotika: Jurnal Ilmiah Biologi Indonesian Journal of Applied Sciences Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biotropika Quagga Bioeksperimen: Jurnal Penelitian Biologi Pro-Life Jurnal Biodjati Bioma : Jurnal Biologi Indonesia Biosaintifika: Journal of Biology & Biology Education Jurnal Riset Akuakultur Jurnal Kreativitas PKM Indonesian Journal of Limnology Manilkara: Journal of Bioscience Journal of Fisheries & Marine
Desak Made Malini
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan, Universitas Padjadjaran
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary

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KONSUMSI OKSIGEN IKAN PELAGIS DI MUARA SEGARA ANAK, TAMAN NASIONAL ALAS PURWO Malini, Desak Made; Muliani, Reni
Bioeksperimen: Jurnal Penelitian Biologi Vol 2, No 2: September 2016
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v2i2.2489

Abstract

Taman Nasional Alas Purwo (TNAP) merupakan salah satu protected area yang ditetapkan sebagai kawasan untuk melestarikan keanekaragaman hayati dan ekosistem. Untuk mencapai tujuan tersebut maka perlu diadakan monitoring tentang kondisi dari kawasan tersebut, yaitu dengan cara mengamati biota perairan seperti ikan pelagis yang habitatnyadi sungai Segara Anak. Kelangsungan hidup ikan ditentukan oleh kondisi lingkungan dan kemampuan ikan untuk menggunakan oksigen dari lingkungannya. Tujuan dari penelitian ini adalah untuk mengetahui jumlah konsumsi oksigen ikan pelagis yang hidup di muara Segara Anak TNAP. Metode yang digunakan adalah observasi. Parameter yang diukur adalah konsumsi O2, kondisi fisik dan kimia dari habitat ikan. Hasil penelitian menunjukkan terdapat 4 jenis ikan pelagis dengan masing-masing konsumsi O2 sebagai berikut Scatophagus argus (0,15 mg/l /h), Toxotes jaculatrix (0,052 mg/l/h), Nectamia fusca (0,052mg/l/h) dan Ambassis macracanthus (0,095 mg/l/h). Tingkat konsumsi oksigen tertinggi ditunjukkan oleh S. argus. Hasil pengukuran parameter fisik dan kimia air adalah sebagai berikut: suhu 28oC, salinitas 33,3%; pH 8; dan oksigen terlarut 5 mg/l, sedangkan suhu udara 30,67oC. Tingkat konsumsi oksigen ikan tergantung pada jenis spesies, ukuran, dan kondisi fisik air.
Alteration of Gills and Liver Histological Structure of Cyprinus carpio Exposed to Leachate Pribadi, Tri Dewi Kusumaningrum; Syahidah, Dzikrina; Harjanti, Sairandri Dyah; Malini, Desak Made
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.8972

Abstract

One of the main problems in the waste management in Indonesia is the treatment of leachate, which mostly dumped to the river This research is aimed to obtain information of histological alteration in gills and liver of C. carpio L. exposed to leachate. Measurements on the water quality parameters comprised water temperature, pH, and dissolved oxygen (DO). This research was conducted by exposing leachate to C. carpio for 96 hours. The concentration of leachate were 0 ppm, 80 ppm, and 100 ppm. Histological preparation were made on the gills and liver using 10% fixative Neutral Buffered Formalin and Ehrlich Hematoxylin-Eosin staining with qualitative observation descriptive analyses for discussion. The result showed that increasing water temperature is directly proportional to the leachate concentration in the aquaria, while the value of pH and DO inversely proportional to the leachate concentration. Damages on the gills with 80 ppm leachate concentrasion were identified as follows: fusion of secondary gill filaments and hyperplasia of epithelial cell, along with karyorrhexis and hydropic degeneration on the liver. Damages on the gills of fishes exposed to leachate with 100 ppm concentrasion were identified as follows: fusion of secondary gill filaments, hyperplasia of epithelial cell, congestion, and edema along with karyorrhexis, hydropic degeneration and melanomacrophage centre (MMC) found on the liver. The results of this study can be used as an overview of the impact of an environmental pollution by leachate as indicated from histological damage to the gills and liver of C. carpio, thus contribute significan information to aquaculture sector and endorse better waste management
Ethnobotanical Study of Medicinal Plants in Karangwangi, District of Cianjur, West Java Malini, Desak Made; Madihah, Madihah; Kusmoro, Joko; Kamilawati, Fitri; Iskandar, Johan
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.5756

Abstract

The knowledge and usage of plant as medicinal remedy by current generation are not as extensive as previous; therefore, many rural communities with restricted modern medical access still rely on traditional medicine. This paper provides significant ethnobotanical information on medicinal plants in Karangwangi Village of Cianjur District, West Java Indonesia. This study aimed to identify plants collected for medical purposes by the local people as well as to document the local names, uses, preparation, and location of these plants. Ethno botanical data was recorded by opting people participation and key informant approach involving semi-structured interviews, group discussions and filling of questionnaires. The results showed a total of 114 medicinal plants belonging to 50 families were identified. Zingiberaceae was the most-frequently cited (nine species), followed by Asteraceae, Euphorbiaceae, and Fabaceae (seven species each). The most-used plant parts were leaves (51.8%), followed by stems (22.9%) and the most common preparations were decoction, poultice and squeezed. Most of the plants were obtained from the house-yard and total of 30 medicinal uses were recorded. The ethnobotanical result documented in this study showed that this area is rich in medicinal plants and these plants are still commonly used for medicinal purposes among the people in their daily lives. Ethnobotanical heritage should be preserved, however, there is a gradual loss of traditional knowledge about these plants in new generation. Further, the findings can be used as baseline information for further scientific investigation for analyzing phytochemical, pharmaceutical and other biological activities for future drug discovery.
Distribution of fish in river is controlled by physico-chemical properties of the water which is affected by land-use complexity and intensity of human intervention. A study on fish distribution was carried out in the upper Citarum River to map the effects of physio-chemical properties on habitat use. A survey was conducted to collect fish and to measure the water quality both on dry and rainy season. The result showed that distribution of the fish, in general, represented their adaptive respons . SUNARDI; KEUKEU KANIAWATI; TEGUH HUSODO; DESAK MADE MALINI; ANNISA JOVIANI ASTARI
HAYATI Journal of Biosciences Vol. 19 No. 4 (2012): December 2012
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.19.4.191

Abstract

Distribution of fish in river is controlled by physico-chemical properties of the water which is affected by land-use complexity and intensity of human intervention. A study on fish distribution was carried out in the upper Citarum River to map the effects of physio-chemical properties on habitat use. A survey was conducted to collect fish and to measure the water quality both on dry and rainy season. The result showed that distribution of the fish, in general, represented their adaptive response to physico-chemical properties. The river environment could be grouped into two categories: (i) clean and relatively unpolluted sites, which associated with high DO and water current, and (ii) polluted sites characterized by low DO, high COD, BOD, water temperature, NO3, PO4, H2S, NH3, and surfactant. Fish inhabiting the first sites were Xiphophorus helleri, Punctius binotatus, Xiphophorus maculatus, and Oreochromis mossambicus. Meanwhile, the latter sites were inhabited by Liposarcus pardalis, Trichogaster trichopterus, and Poecilia reticulata. Knowledge about fish distribution in association with the pysico-chemical properties of water is crucial especially for the river management.
Treatment of PLGA Nanoparticles Ointment-Ethanol Extract of Archidendron pauciflorum in the Wound Healing in Diabetic Mice Desak Made Malini; Yasmi Purnamasari Kuntana; Madihah Madihah; Wildanul Furqon; Wawan Hermawan
Jurnal Biodjati Vol 5, No 2 (2020): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v5i2.9256

Abstract

Diabetic wounds lead to severe tissue damage and are diffi-cult to cure. Jengkol (Archidendron pauciflorum) is a plant commonly used by local Indonesian communities to treat diabetic wounds. The efficiency of herbal medicine still has a deficiency of its ability to reach the target organs, therefore nanotechnology is applied in the hope that all drug concentrations can reach the target organs successfully. This study aimed to evaluate the effectiveness of PLGA nanoparticle ointment-ethanol extract jengkol fruit peel (EEJFP) to accelerate the wound healing process in the skin of streptozotocin-induced diabetic mice. The research method used was experimental with a completely randomized design using six treatments and four replications. Diabe-tes was induced by intraperitoneal injection of streptozotocin 180 mg/ kg BW. Mice with a blood glucose level of ≥150 mg/dL were used for diabetic mice models. The incision wound created at the dorsolateral region of shaven skin at ±1 cm2 using sterile scissors. The treatments given were vaseline for Control Negative (CN) and Control Positive (CP), Betadine ointment (PB), 10% EEJFP ointment (P1), 5% PLGA nanoparticle ointment-EEJFP (P2), and 2.5% PLGA nanoparticle ointment-EEJFP (P3). The results showed that the administration of PLGA nanoparticles ointment-EEJFP with a concentration of 5% PLGA nanoparticle ointment-EEJFP (P2) resulted in the shortest wound length on day 3, 7 and 14; narrower granulation tissue; a larger number of blood capillaries; and denser collagen fibers (α <0.05) compared to CP and PB treatments. The administration of PLGA nanoparticle ointment-EEJFP with a concentration of 5% was the most effective concentration in accelerating wound healing in the skin of diabetic mice. 
AKTIVITAS ANTIFIDAN EKSTRAK ETANOL DAUN TANAMAN Piper nigrum, Acorus calamus, DAN Dysoxylum alliaceum TERHADAP LARVA Spodoptera litura INSTAR III Hikmat Kasmara; Melanie Melanie; Vita Novianti; Madihah Madihah; Desak Made Malini; Rani Maharani; Tri Mayanti; Wawan Hermawan
BIOTIKA Jurnal Ilmiah Biologi Vol 16, No 1 (2018): BIOTIKA JUNI 2018
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/bjib.v16i1.17481

Abstract

TINGKAH LAKU SEKSUAL DAN UJI KAWIN MENCIT (MUS MUSCULUS) JANTAN YANG DIINDUKSI STREPTOZOTOCIN Desak Made Malini; Dea Rahman Khairunnisa; Nurullia Fitriani; Nining Ratningsih
BIOTIKA Jurnal Ilmiah Biologi Vol 19, No 1 (2021): BIOTIKA JUNI 2021
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/biotika.v19i1.32703

Abstract

Diabetes melitus (DM) merupakan salah satu penyakit degeneratif yang dapat menyebabkan terjadinya impotensi, gangguan ejakulasi, spermatogenesis dan fungsi kelenjar seks aksesori serta libido. Streptozotocin (STZ) merupakan salah satu diabetogenik yang banyak dimanfaatkan sebagai metode untuk merangsang DM pada hewan percobaan.Tujuan dari penelitian ini adalah untuk mengetahui pengaruh STZ terhadap tingkah laku seksual dan kemampuan kawin mencit (Mus musculus). Penelitian ini dilakukan secara rancangan eksperimental dengan 2 kelompok perlakuan dan 5 ulangan, yaitu perlakuan non diabetes (ND) yang tidak diinduksi STZ dan diabetes (D) yang diinduksi STZ. Hewan uji yang digunakan adalah mencit jantan dan betina. Parameter yang diamati adalah tingkah laku seksual mounting dan kissing vagina oleh mencit jantan, berat badan mencit betina dan jumlah fetus yang dihasilkan oleh mencit betina. Hasil pengamatan dianalisis dengan Uji Chi-Square dan uji lanjut Kruskall Wallis (uji lanjut Mann-Whitney). Hasil penelitian menunjukkan bahwa pada mencit jantan yang diinduksi STZ terjadi penurunan tingkah laku seksual, dan tidak ditemukan adanya implantasi pada uterus betina pasangan kawinnya. Dapat disimpulkan bahwa induksi STZ dapat menurunkan tingkah laku seksual mencit jantan dan tidak mampu mengawini mencit betina.
MORFOMETRIK KERANG HIJAU (Perna viridis) DAN REMIS (Donax compressus) DI PERAIRAN PANTAI TIMUR PANGANDARAN, JAWA BARAT Desak Made Malini; Emilie Nuraida Erawan
BIOTIKA Jurnal Ilmiah Biologi Vol 17, No 2 (2019): BIOTIKA DESEMBER 2019
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/biotika.v17i2.26006

Abstract

Pantai Timur Pangandaran merupakan salah satu lokasi pariwisata yang berada di dekat Cagar Alam Pananjung Pangandaran dan merupakan habitat bagi kerang hijau (Perna viridis) dan remis (Donax compressus). Organisme tersebut sangat rentan terhadap perubahan factor lingkungan seperti salinitas, pH, ketersediaan nutrisi dan tingkat pencemaran. Tujuan penelitian ini adalah untuk mengetahui korelasi antara panjang cangkang, lebar cangkang, berat daging dengan berat total dari P. viridis dan D. compressus serta hubungannya dengan kondisi lingkungan di perairan Pantai Timur Pangandaran. Metode penelitian yang digunakan adalah dengan teknik survey. Sampel P. viridis diperoleh di area keramba jaring apung sedangkan sampel D. compressus diperoleh di sepanjang pesisir pantai. Hasil penelitian menunjukkan bahwa nilai r tertinggi pada P. viridis terdapat pada korelasi berat daging dengan berat total yaitu r 0,9864 dan memiliki nilai R sebesar 0,9731, sedangkan pada D. compressus nilai r tertinggi sebesar 0,97613 dengan R2 0,952. Nilai terendah pada P. viridis berada pada nilai r 0,8330 dengan R2 0,6939, dan pada D. compressus nilai r 0,8747 terendah dengan R2 0,7652. Berdasarkan pada hasil pengukuran morfometrik P. viridis dan D. compressus dapat disimpulkan bahwa perairan Pantai Timur Pangandaran belum tercemar.
Pengaruh Ekstrak Etanol dan Spinasterol Daun Senggugu (Clerodendron Serratum L.) terhadap Kualitas Sperma Mencit (Mus Musculus L.) Desak Made Malini
Indonesian Journal of Applied Sciences Vol 3, No 3 (2013)
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8715.33 KB) | DOI: 10.24198/ijas.v3i3.15042

Abstract

AbstrakTumbuhan senggugu (C. serratum L.) termasuk famili verbenaceae, merupakan salah satu tumbuhan yang cukup dikenal dimasyarakat sebagai tanaman obat. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak daun senggugu (C. serratum L.) terhadap kualitas sperma mencit (Mus musculus). Penelitian ini merupakan penelitian eksperimental yang menggunakan rancangan percobaan dengan pola Rancangan Acak Lengkap (RAL). Penelitian ini terdiri atas dua kelompok kontrol dan lima kelompok perlakuan dengan lama perlakuan 9 dan 18 hari. Setiap kelompok menggunakan 6 ekor mencit, sehingga jumlah mencit keseluruhan adalah 42 ekor. Parameter yang diamati adalah jumlah, motilitas, abnormalitas dan viabilitas sperma mencit. Data yang diperoleh dianalisis dengan Anava dan dilanjutkan dengan uji Duncan. Hasil penelitian ini menunjukkan bahwa dilihat dari hasil daun senggugu (C. serratum L.) dapat menurunkan kualitas mencit.Kata kunci : senggugu, mencit dan kulaitas sperma.AbstractPlant senggugu (C.serratum L.) belongs to the family Verbenaceae, which has been recogniced by the public as a medicinal plant. One of them is that it can be used as a drug antifertility in men. The purpose of this study is to find out the effect of ethanol extract and spinasterol of Senggugu ©.Serratum L.) leaves to the sperm quality of mouse (M. musculus) The study is conducted using complete random with 7X2 factor repeated 6 times. The treatment given to the ethanol extract is 250 mg/kg body weight, 500 mg/kg body weight and 1000 mg/kg body weight, as with spinasterol the amount is 26 mg/kg body weight and 52 mg/kg body weight, and as its controlling agent distilled water and DMSO solution is used. The treatment is given orally once a day within 9and 18 days as much as 0,5 ml. Cauda epididymal sperm suspension was used to measure the quality of the sperm of mice. Data were analyzed by ANOVA followed by Duncan test. Based on observation and statistical analysis it has been found that theethanol and spinasterol extract treatment of senggugu leaves using a diffrent dosage has shown a significant effects to the parameters being studied namely increased abnormality of sperm and decreased in number of sperm, percentage of motolityand viability sperm.Keyword : Clerodendron serattum, senggugu, Mus musculus, Spermatozoa, Ethanol extract, Spinasterol
Perilaku Kawin Tikus (Rattus norvegicus) Jantan Diabetes Yang Diberi Ekstrak Etanol Kulit Buah Jengkol (Archidendron pauciflorum) Desak Made Malini; Emay Maulani; Sri Wulandari; Nining Ratningsih
Metamorfosa: Journal of Biological Sciences Vol 7 No 2 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i02.p17

Abstract

Diabetes mellitus (DM) may lead to disruption of normal sexual function in males including abnormalities of orgasmic/ejaculatory function and desire/libido. Simplisia of dried Jengkol fruit peel has been used by people to treat diabetes. The aim of this study was to examine the effectiveness of ethanol extract of jengkol fruit peel (A. pauciflorum) (EEJFP) in improving the sexual behavior of male rats (R. norvegicus) diabetic models. Induction of diabetes was carried out intravenously by using streptozotocin dose 60 mg/ kg BW in the test animals except for the negative control group.The treatments are negative control (Carboxy Methyl Cellulose (CMC) 0.05%), positive control (CMC 0.05%), P1 (EEJFP 385 mg/kg BW), P2 (EEJFP 770 mg/kg BW) and comparison (glibenclamide dose 10 mg/kg BW) for 54 days. The parameters observed are the number of introduction and climbing of male rats to female rats. Data obtained from each parameter were analyzed by analysis of variance (ANAVA) test with a 95% confidence level and Duncan's test. The results of this study indicate that the number of introductions and climbing in the P1 and P2 test animal groups are significantly different from the positive control treatment and close to the value in the negative control treatment group. It can be concluded that the ethanol extract of jengkol fruit peel (A. pauciflorum) affects the sexual behavior of male rats (R. norvegicus) diabetes models by increasing introduction and climbing. Keyword: diabetic, sexual behavior, Archidendron Pauciflorum, rats
Co-Authors . SUNARDI Adam Robisalmi, Adam Adnin Laila Amalia Shalihah Andi Perdana Anggraeni, Fajar ANNISA JOVIANI ASTARI Artati, Diah Asep Sopian Azalea Putri Bisri Mustofa Camellia Panatarani Damanhuri, Joni Haryadi Dea Rahman Khairunnisa Dita Aprila Khoirunnisa Dwi Rahmi Dzikrina Syahidah, Dzikrina Elsa Dwi Fauzany Emay Maulani Emilie Nuraida Erawan Fajar Anggraeni Fajar Anggraeni Firda Mohammad Firdous Hary Krettiawan Hikmat Kasmara I Made Joni Iin Supartinah Ikhsan Khasani Imron Imron Imron Imron, Imron Indriani Sasmita Johan Iskandar Joko Kusmoro Joko Kusmoro Kabul Fadilah Kamilawati, Fitri Kartiawati Alipin KEUKEU KANIAWATI Keukeu Kaniawati Rosada Khairul Syahputra, Khairul Kuntana, Yasmi Purnamasari Listiyowati, Nunuk Madihah Madihah Madihah Madihah Madihah Madihah Madihah Madihah Madihah Madihah Madihah Madihah, Madihah Melanie Melanie Melanie Melanie, Melanie Mia Miranti Rustama Miranti Ariyani Muliani, Reni Muliani, Reni Nadia Istiqomah Nana Media Nia Rossiana Nining Ratningsih Nining Ratningsih Nining Ratningsih Nining Ratningsih Nurullia Fitriani Octaviani, Regita Perdana, Andi Permana, Andre Dian Putri, Deviana Aulia Rani Maharani Reni Muliani Resty Agustin Ruly Budiono Sairandri Dyah Harjanti, Sairandri Dyah Sofia Dorothy SRI WULANDARI sunardi sunardi Supartinah, Iin Syifa Yolanda TEGUH HUSODO TEGUH HUSODO Tia Setiawati Tia Setiawati Tia Setiawati Tri Dewi K. P Tri Dewi Kusumaningrum Pribadi Tri Mayanti Virita Rossa Pratiwi Vita Novianti Wahyu Pamungkas Wawan Hermawan Wawan Hermawan Wawan Hermawan Wawan Hermawan Wawan Hermawan Wildanul Furqon Yasmi Purnamasari Kuntana