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All Journal ACTA VETERINARIA INDONESIANA Buletin Veteriner Udayana
Nyoman Sadra Dharmawan
Center For Study Of Animal Disease (CSAD), Fakultas Kedokteran Hewan, Universitas Udayana, Jl. Raya Sesetan Gg. Markisa No. 6, Denpasar Selatan
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Deteksi Infeksi Anaplasma sp., Borrelia burgdorferi dan Ehrlichia sp. pada Anjing yang Terinfestasi Caplak di Kota Denpasar Ni Made Devityasih Perayadhista; Nyoman Adi Suratma; Nyoman Sadra Dharmawan
Buletin Veteriner Udayana Vol. 14 No. 5 October 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2022.v14.i05.p16

Abstract

This research has been conducted as its purpose is to detect the infection of Anaplasma sp., Borrelia burgdorferi, and Ehrlichia sp. in dogs as well as it was manifested with ticks in Denpasar City. The detection of these parasites was conducted by serological examination, haematology test, and microscopic identification with blood smear. Blood samples were examined from 30 dogs which is belong to local society and it also invested with Rhipichepalus sanguineus. Serological examination was performed with SNAP® 4DX® Plus test kit. Haematology examination were using haematology analyzer I-CUBIO iCell-800 Vet®. The risk factor was learned by analyzing it with correlation to parasite infection case in dogs with a few factors which is considered being influenced. The result of serological examination shows that 22 of 30 dogs (73.3%) were positively reacted to Anaplasma sp. and Ehrlichia sp. but shown negative to Borrelia burgdorferi. In addition, haematology profile shown anemia, leucocytosis, leucopenia, lymphocitosis, lymphopenia, and thrombocytopenia, as well as abnormalities in blood cell morphology such as: poikilocytosis, and the presence of crenation. There is no correlation between blood pathogenic agents in this research to: age, breed, sex, origin, presence of other pets, or grooming management.
Profil Hematologi dan Biokimia Darah Babi Bali Asal Peternakan Tradisional dan Konvensional yang Diberi Pakan Komersial Putu Vindhy Chempaka Putri; I Gede Mahardika; Nyoman Sadra Dharmawan
Buletin Veteriner Udayana Vol. 14 No. 5 October 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2022.v14.i05.p14

Abstract

Experimental studies have been carried out to determine the weight gain, blood hematological and biochemical profile of weaning bali pig from traditional and conventional farms which are then given the same treatment as commercial feed. Weighing pigs is carried out every two weeks for two months. Examination of blood samples was carried out three times, at the beginning, middle and end of the study. The hematology profile was examined with a hematology analyzer. Cholesterol, triglycerides, and protein total were examined by photometer. The results showed that the weight gain of weaning pigs from traditional and conventional farms for 8 weeks was 13.81 kg and 14.19 kg (P>0.05). Overall range of erythrocytes; hematocrit; and hemoglobin in this study were 7.47-8.59 x 106/µL; 37.23-44.78 %; and 11.95-14.17 g/dL, respectively. The total leukocyte range is 19.12-28.18 x 103/µL; neutrophils: 0.49-7.19 %; eosinophils: 0.24-2.0 %; basophils: 0.73-5.18 %; lymphocytes: 16.22-83.02 %; and monocytes 1.21-6.82 %. The cholesterol range is 65.85-90.00 mg/dL; triglycerides: 30,75-78,81 mg/dL; and protein total: 5.0-6.6 g/dL. There was no statistically significant difference (P>0.05) between the average erythrocytes, hematocrit, hemoglobin, leukocytes total, leukocytes differential, cholesterol and protein total of piglets originating from traditional farm compared to those from conventional farm. However, there were significant differences (P<0.05) in triglyceride levels.
Aktivitas Aalanine Transaminase dan Aspartate Transaminase pada Babi yang Terdeteksi Sistiserkosis Secara Serologi Nyoman Sadra Dharmawan; I Gede Mahardika; Kadek Swastika; Kadek Karang Agustina
Acta VETERINARIA Indonesiana Vol. 10 No. 3 (2022): November 2022
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.10.3.239-244

Abstract

Sistiserkosis adalah penyakit infeksi yang disebabkan oleh larva (cysticercus) cacing pita. Sistiserkosis pada babi disebabkan oleh Cysticercus Taenia solium yang berparasit pada otot, hati, otak, dan mata babi. Bentuk dewasanya ada dalam usus manusia, disebut T. solium. Babi juga dapat terinfeksi Cysticercus T. hydatigena yang berparasit pada hati, peritoneum, omentum dan mesenterium. Bentuk dewasanya, T. hydatigena, berparasit pada usus anjing. Cysticercus yang berkembang pada hati babi menyebabkan kerusakan hati. Tujuan penelitian ini adalah membandingkan nilai biokimia darah pada babi yang secara serologis terdeteksi sistiserkosis dengan yang tidak terdeteksi. Paramater yang diamati meliputi aktivitas enzim alanine transaminase (ALT) dan aspartate transaminase (AST). Deteksi serologi sistiserkosis dilakukan dengan enzyme linked immunosorbent assay (ELISA), menggunakan antigen glikoprotein; aktivitas enzim ALT dan AST diperiksa dengan uji spektrofotometri UV. Hasil penelitian menunjukkan bahwa nilai ALT pada babi yang positif terdeteksi sistiserkosis adalah 57,7 + 4,96 µ/l dan untuk babi yang negatif adalah 53,6 + 3,71 µ/l. Nilai AST pada babi yang positif terdeteksi sistiserkosis adalah 53,8 + 13,38 µ/l dan untuk babi yang negatif adalah 52,7 + 12,01 µ/l. Pada penelitian ini, walaupun masih dalam rentang nilai normal, tampak rataan nilai ALT dan AST pada babi yang terdeteksi sistiserkosis lebih tinggi dibandingkan dengan yang tidak terdeteksi.
Co-Authors I Gede Mahardika I Kadek Swastika I NYOMAN ADI SURATMA Kadek Karang Agustina Ni Made Devityasih Perayadhista Putu Vindhy Chempaka Putri