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All Journal Agroland : Jurnal Ilmu-Ilmu Pertanian AGROLAND: The Agricultural Sciences Journal
Hawalina Hawalina
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Agriculture, Biological Sciences & Forestry

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PERTUMBUHAN TANAMAN BUAH NAGA (Hylocereus undatus L.) YANG DIBERIKAN BERBAGAI KONSENTRASI NAA (Napthalen Acetic Acid) SECARA IN VITRO Ramal Yusuf; Syamsuddin Laude Laude; Hawalina Hawalina; Ni Made Setianingsih
Agroland: Jurnal Ilmu-ilmu Pertanian Vol 24, No 2 (2017)
Publisher : Universitas Tadulako

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Abstract

The research aimed to gain the appropriate NAA concentration in performing tissue culture of dragon fruit plants. The research was compiled by using completely randomized design consisting of 4 treatments and each treatment was repeated 6 times that 24 experimental units were done. The variables observed include the number of shoots, the number of roots, the number of segments, and the length of shoots observed at the age of 4 weeks and 8 weeks after planting. The research findings reveal that on the observation at 4 weeks after planting the NAA concentration has effect on the number of segments and the elongation of shoots, yet it does not have effect on the number of shoots and roots, while at 8 weeks after planting the NAA concentration has effect on the number of shoots, the number of roots, the number of segments, and the length of shoots. The research findings also show that the treatment of N3 (0.3 ppm NAA) gives better result compared with other treatments in which the average number of shoots formed is 5.50 per explant, the number of roots formed is 3.50 per explant, the number of segments formed is 8.83 per explant and the average length of shoots formed is 2.13 cm per explant. Key Words : Dragon Fruit, NAA Concentration, In Vitro.
INITIATION OF GRAPE (VITIS VINIFERA L.) ON DIFFERENT STERILIZATION TECHNIQUES Ratu Mentari Dewi; Ramal Yusuf; Sri Anjar Lasmini; Hawalina Hawalina
AGROLAND: The Agricultural Sciences Journal Vol 5, No 2 (2018)
Publisher : Tadulako University

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The most crucial step during the development of tissue culture is the method of explant sterilization. Especially, explants is sourced directly from fields that were more susceptible to microbial contaminations. This study aimed to obtain sterilization technique  from several sterilant for reduced contamination and support growth of shoots grape in vitro. This research was conducted at the Laboratory of Plant Biotechnology, Faculty of Agriculture, University of Tadulako, during January to April 2018. The study used a factorial completely randomized design, one factor was tested i.e S1 = Taft 8,3 g/L (0,1% carbendazim), S2 =  taft 25 g/L (0,3% carbendazim),  S3 = taft 41,67 g/L (0,5% carbendazim), S4 = Taft 8,3 g/L (0,1% carbendazim) + HgCl2 0,1%, S5  = Taft 25 gr/L (0,3% carbendazim) + HgCl2 0,1% dan S6 = 41,67 gr/L  (0,5% carbendazim) + HgCl2 0,1 %. There were six treatment and each treatment was replicated three times to obtain 18 experimental units. Each experimental unit using two explant. Data were analyzed using analysis of variance and followed by Honestly Significant Difference test at level of 5% if the treatment effects were significant. The results showed sterilization technique using taft 41,67 gram / L containing 0.5% carbendazim for 30 minutes, followed by 70% alcohol for 30 seconds and HgCl2 0,1% for 10 minutes gave free contamination for explant.whereas, Taft 8.3 g / L (Carb 0.1%) for 30 min, followed by 70% alcohol for 30 seconds is a sterilization technique that does not inhibit explant growth as indicated from the most callus formation.
THE GROWTH OF KIWI SHOOT (ACTINIDIA DELICIOSA) ON VARIOUS KINDS OF GELLING AGENTS Mardiana Mardiana; Zainuddin Zainuddin; Mahfudz Mahfudz; Hawalina Hawalina
AGROLAND: The Agricultural Sciences Journal Vol 5, No 2 (2018)
Publisher : Tadulako University

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Abstract

Kiwi fruit takes about 25 weeks from flower bloom until it reaches physiological maturity, so the time required to produce kiwi seeds from seeds in large quantities and uniform is very long. Tissue culture is one method that can be used to obtain a lot of kiwi seeds and uniforms with large quantities in a faster time. The purpose of this study was to examine various types of media compaction materials for the growth of kiwi shoots in vitro. This study was prepared based on Completely Randomized Design (RAL) with 5 treatments and repeated 4 times so that there were 20 experimental units, each experiment using 2 explants so that there are 40 eksplan. The treatments were: MA 1: Agar Swallow Globe 8 g / l, MA 2: Agar Swallow Globe 4 g / l + Agar Nutrijell 4 g / l, MA 3: Agar Swallow Globe 4 g / l + Agar Nutrijell 5 g / l, MA 4: Phytagel 2.2 g / l, MA 5: Agar Nutrijell 11 g / l. Observation variables are When shoots appear, Number of shoots, number of leaves, Number of Roots, number of root hair. The results showed Swallow Globe 4 g / l + Agar Nutrijell 4 g / l treatment gave the highest average number of shoots, the highest number of leaves and roots, this proved that the combination of Swallow Globe and Nutrijell agar gave a good condition for shoot growth kiwi plant.t.
CALLUS INDUCTION OF CACAO CLONE SULAWESI 1 ON VARIOUS CONCENTRATIONS OF 2,4 -D AND COCONUT WATER VIA IN VITRO CULTURE Asmila Asmila; Zainuddin Basri; Ramal Yusuf; Hawalina Hawalina
AGROLAND: The Agricultural Sciences Journal Vol 4, No 1 (2017)
Publisher : Tadulako University

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Abstract

Cacao is one of important plantation crops grouped in the Sterculiaceae family.  Sulawesi is the main area of cacao production and has a number of superior clones, such as Sulawesi 1 and Sulawesi 2.  Based on data in 2012/2014 cacao production to consumption reached 174,000 tons, while in 2013/2014 was projected a deficit of 115,000 tonnes. Nonetheless, cacao agribusiness in Indonesia is still facing complex problems, among others gardener productivity is still low due to borer attacks cacao, the quality of products and the number is still low and still not optimal development of cacao products and providing superior amount of cacao seedlings. The primary problem of cacao production recently is low productivity.  The main cause of low cacao productivity in Central Sulawesi is the use of inferior clones.  To enhance cacao productivity, it is crucial to use cacao clones having high genetic potential via tissue culture or micropropagation techniques.  The aim of this experiment was to assess the effect of different concentrations of 2,4-D and coconut water on the growth of cacao callus via in vitro culture.  This experiment used Completely Randomozed Block Design in factorial patteren with treatments tested namely 2,4-D and coconut water concentrations.  The concentrations of 2,4-D tested including 1 ppm, 2 ppm and 3 ppm, whilst coconut water concentrations tested consisting of 10%, 15% and 20%, and therefore there were 3 x 3 = 9 treatment combinations.  Each treatment utilized 4 replications; and each unit combination used 5 explants (staminodia).  Results of this experiment indicated that the addition of 3 ppm 2,4-D and 10% coconut water had a highly significant effect on the callus color 4 weeks after culture.  The addition of 3 ppm 2,4-D in culture media showed a significant effect on callus color 4 weeks after culture, but had an insignificant effect on the callus formation, callus color 8 weeks after culture an callus texture.  Supplementation of 20% coconut water had a significant effect on callus texture 8 weeks after culture, whilst the addition of 10% coconut water showed a significant effect on callus color 4 weeks after culture.
PERTUMBUHAN TUNAS ANGGUR HITAM (Vitis vinifera L.) PADA BERBAGAI KONSENTRASI BENZYLAMINO PURIN DAN INDOLEBUTYRIC ACID Mardiyah Mardiyah; Zainuddin Basri; Ramal Yusuf; Hawalina Hawalina
Agroland: Jurnal Ilmu-ilmu Pertanian Vol 24, No 3 (2017)
Publisher : Universitas Tadulako

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Abstract

This study aimed to determine media compositions supplied with BAP and IBA suitable for the growth of black grape shoots. This research was conducted at the Laboratory of Plant Biotechnology, Faculty of Agriculture, University of Tadulako, during April to September 2016. The study used a factorial completely randomized design. Two factors were tested, the concentrations of BAP i.e. 1.50 ppm, 2.00 ppm and 2.50 ppm; and the concentration of IBA i.e. 0.00 ppm, 0.25 ppm and 0.50 ppm. There were nine treatment combinations and each combination treatment was replicated three times to obtain 27 experimental units. Each experimental unit using an explant. Data were analyzed using analysis of variance and followed by Honestly Significant Difference test at level of 5% if the treatment effects were significant. The results showed that the composition of the culture media supplied with 2.00 ppm BAP without IBA is able to stimulate the formation of black grape leaves at the earliest, at an average of 18.67 days per explant, while the media added with 2.00 ppm 0.25 ppm BAP together with IBA can lead to longest shoot growth, at an average of 2.13 cm per bud. The culture media applied with 2.50 ppm BAP can trigger earliest shoot growth and highest number of leaf formation which were 11.11 days after culture and 2.44 leaves per explant. Earliest formation of black grape shoots of 11.89 days after culture is stimulated by the addition of 0.25 ppm IBA.
Co-Authors Asmila Asmila Mahfudz Mahfudz Mardiana Mardiana Mardiyah Mardiyah Ni Made Setianingsih Ramal Yusuf Ratu Mentari Dewi Sri Anjar Lasmini Syamsuddin Laude Zainuddin Basri Zainuddin Zainuddin