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Lilis Siti Aisyah

Department of Chemistry, Faculty of Science and Informatics, Jenderal Achmad Yani University Cimahi

Author-ID : 5301097

Chemical Engineering, Chemistry & Bioengineering Chemistry Engineering

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Articles

The Effectiveness of Adding Red Fruit Oil (Pandanus conoideus Lamk.) into Ethanol Extract of Temulawak rhizome (Curcuma xanthorrhiza Roxb.) as Antioxidant Sari Purbaya; Lilis Siti Aisyah; Dona Nopitasari
Jurnal Kimia Sains dan Aplikasi Vol 23, No 11 (2020): Volume 23 Issue 11 Year 2020
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Curcuma xanthorrhiza Roxb (temulawak) is a native Indonesian plant; ethnobotanically, it can facilitate breast milk, body fresheners, stomach lozenges, and seizure medications. Temulawak contains essential oils, curcuminoids, and xanthorrhizol. Curcuminoids and xanthorrhizol have potential as antioxidants. Antioxidants in the body are useful for preventing oxidation reactions caused by free radicals, both from body metabolism and external factors. Curcuminoids are difficult to dissolve in water and have a very low bioavailability. Combining curcuminoids with oil can increase the absorption of curcuminoids into the systemic system. The low solubility and bioavailability can be overcome by combining temulawak rhizome extract and red fruit oil. Pandanus conoideus Lamk (red fruit), ethnobotanically, effectively prevents blindness, intestinal worms, skin diseases, and increasing stamina. Red fruit contains vitamin C, vitamin E, flavonoids, and β-carotene, potentially as antioxidants. Each sample of Curcuma and red fruit, as well as a mixture of Curcuma and red fruit samples, were tested for their antioxidant activity using the DPPH method (1,1-diphenyl-2-picrylhydrazyl). The ethanol extract of temulawak rhizome has intense antioxidant activity because it has an IC50 of 55.21 ppm. Red fruit oil has feeble antioxidant activity because it has an IC50 of 2604.77 ppm. The 1: 1 ratio between red fruit oil and the ethanol extract of temulawak rhizome has weak antioxidant activity because it has an IC50 of only 1568.24 ppm. Meanwhile, the 1:10 ratio of red fruit oil and ethanol extract of temulawak rhizome has robust antioxidant activity because it has an IC50 of 19.85 ppm. It is concluded that the addition of oil to the ethanol extract of Curcuma can increase antioxidant activity.

α-Amylase Inhibition Activities by Flavonoid Compounds from Panda Plants (Kalanchoe tomentosa) Lilis Siti Aisyah; Delia Ilfani; Faridia Puspita Lestari; Yenny Febriani Yun
Jurnal Kimia Sains dan Aplikasi Vol 23, No 3 (2020): Volume 23 Issue 3 Year 2020
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Diabetes mellitus (DM) is one of the major health problems. One way to deal with diabetes mellitus is by inhibiting the work of enzymes that hydrolyze carbohydrates, thereby reducing glucose absorption. The enzyme that plays a role in the breakdown of starch into simple sugars is the α-amylase. The genus Kalanchoe or Panda plant has been widely reported to contain a variety of secondary metabolites that have several activities such as antimalarial, antibacterial, antidiabetic, and anticancer potential. This study aims to isolate, determine the chemical structure, and test the inhibitory activity of α-amylase from the ethyl acetate fraction of K. tomentosa leaves. The isolation stage began with the maceration process with n-hexane and fractionated with ethyl acetate. Ethyl acetate extract was refracted using a gradient liquid vacuum chromatography (KCV) method (n-hexane: ethyl acetate: methanol). Repeated gravity columns separated the fraction of KCV results and preparative thin-layer chromatography (TLC-P) to obtain isolate pure. Isolate pure was characterized using several spectroscopic methods, including UV-Vis, IR, NMR. Determination of the structure of the compound against isolate pure shows that this isolate is a 3,7,4’-trihydroxyflavonol or kaempferol compound. The results of the test activity of 3,7,4’-trihydroxyflavonol compounds in inhibiting the enzyme α-amylase in vitro showed an IC50 value of 346 µU/mL. The results obtained indicate that the IC50 value is higher than that of the Acarbose, which was 39.3 µU/mL.

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