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All Journal HAYATI Journal of Biosciences
Mukh Fajar Nasrulloh
Research Center For Biotechnology, Indonesian Institute Of Sciences, Bogor, West Java, 16911
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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Molecular Detection of Eimeria bovis in Indonesian Beef Cattle Using Nested PCR Technique Nasrulloh, Mukh Fajar; Nurcahyo, Raden Wisnu; Priyowidodo, Dwi; Ekawasti, Fitrine; Firdausy, Lintang Winantya
HAYATI Journal of Biosciences Vol. 31 No. 5 (2024): September 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.5.980-987

Abstract

Eimeria bovis is a pathogenic protozoan that causes cattle digestive tract infections, which can cause economic losses to farmers. It is necessary to develop specific and accurate detection methods to conserve livestock and prevent coccidiosis in Indonesia. This study aims to detect E. bovis by nested PCR and determine the relationship with reference sequences. A total of 167 samples of beef cattle feces were taken randomly from community farms spread across 18 provinces in Indonesia. The feces were examined natively, and then the oocysts were purified by the sugar flotation method, extracted by KIT extraction, and amplified by the nPCR technique. Positive samples were followed by sequencing and phylogenetic analysis using MEGA 11 software. This study used two pairs of primers (outer and inner) taken from ITS-1 molecular markers. As many as 96 out of 167 samples (57.5%) were positive for Eimeria spp., and 48 of the 96 samples were positive for Eimeria spp. (50%) were detected to be positive for E. bovis based on the presence of a 238 bp DNA fragment. The results of the phylogenetic analysis showed that the study sample formed a separate cluster from the E. bovis cluster from abroad. In conclusion, E. bovis was detected in 16 out of 18 provinces in this study, and the nPCR technique proved to have better sensitivity and specificity.
Co-Authors Ari Sulistyo Wulandari Ari Sulistyo Wulandari Dwi Priyowidodo Endang Tri Margawati Endang Tri Margawati Firdausy, Lintang Winantya Fitrine Ekawasti, Fitrine Ika Rahmawati Indriawati Indriawati Indriawati Indriawati Muhammad Cahyadi Nurcahyo, Raden Wisnu Pita Sudrajad Slamet Diah Volkandari Slamet Diah Volkandari