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All Journal HAYATI Journal of Biosciences Livestock and Animal Research
Mukh Fajar Nasrulloh
Research Center For Biotechnology, Indonesian Institute Of Sciences, Bogor, West Java, 16911
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Veterinary

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Effect of polymorphism of Insulin-induced gene 1 (INSIG1) (A4366G) on slaughter characteristics in unproductive Kebumen Ongole Grade cows Slamet Diah Volkandari; Saiful Anwar; Ari Sulistyo Wulandari; Pita Sudrajad; Indriawati Indriawati; Mukh Fajar Nasrulloh; Ika Rahmawati; Endang Tri Margawati; Muhammad Cahyadi
Livestock and Animal Research Vol 19, No 2 (2021): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (623.265 KB) | DOI: 10.20961/lar.v19i2.48557

Abstract

Objective: Kebumen Ongole Grade (Kebumen OG) are known as good performance cattle with body weight and body measurement higher than National Standard of Ongole Grade cattle. Productivity is influenced by genetic and environmental factors. Insulin-induced gene-1 (INSIG1) gene is one of many genes that are considered important in influencing carcass characteristics and playing an important role in lipid metabolism (adipogenesis and or lipogenesis). So, the aim of this study was to identify of SNP A4366G in INSIG1 gene and associated with slaughter characteristics of unproductive Kebumen OG cows.Methods: In this study used 44 unproductive Kebumen OG cows from slaughter house in Kebumen. Slaughter characteristics contain of slaughter weight, hot carcass weight and dressing percentage. Three milliliters of blood samples were collected from vena jugularis. DNA were extracted from blood samples using salting out method. Genotyping of INSIG1 gene (A4366G) used PCR-RFLP method with TaqI restriction enzyme. Genetic diversity data in this study were allele and genotype frequencies, heterozygosity, PIC and HWE. Association of genotypes of INSIG1 gene with slaughter characteristics were analyzed using ANOVA univariate model.Results: Polymorphic of INSIG1 (A4366G) gene was found in the unproductive Kebumen OG cows. Three variants of genotypes (AA, AG, GG) with two alleles (A and G) were found with allele frequencies 0.795 for G allele. The population was in equilibrium genetic. Association of slaughter characteristics with genotypes were not significant (p>0.05).Conclusions: Polymorphic of INSIG1 gene (A4366G) was found in unproductive Kebumen OG cows population with dominant of G allele. The population was in genetic equilibrium. The association of slaughter characteristics with genotype of INSIG1 gene (SNP A4366G) was not significant.
Detection of the CD18 gene mutation as a marker of BLAD genetic disorder of Holstein-Friesian cattle in West Java Mukh Fajar Nasrulloh; Ari Sulistyo Wulandari; Indriawati Indriawati; Endang Tri Margawati; Slamet Diah Volkandari
Livestock and Animal Research Vol 18, No 2 (2020): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (422.154 KB) | DOI: 10.20961/lar.v18i2.42933

Abstract

Objective: Bovine Leukocyte Adhesion Deficiency (BLAD) is a genetic disorder in Holstein-Friesian (HF) cattle that have a significant economic impact on dairy cattle breeding. Mutation c.383A>G in Cluster of Differentiation Molecule 18 (CD18) gene was known as related to BLAD. This study aimed to detect the CD18 gene mutation that causes BLAD of HF cattle in the West Java Province.Methods: The genomic DNA was extracted from 88 blood samples of HF cattle from Cibungbulang-Bogor (n=34), Ciampea-Bogor (n=31), and Sukabumi (n=23) by the GB300 DNA extraction kit, GeneaidTM. The CD18 gene mutation was detected by PCR-RFLP analysis using the TaqI restriction endonuclease.Results: All samples in this study produced two fragments DNA, i.e., 359 bp and 260 bp with the monomorphic homozygote genotype (BB). HF cattle with BB genotype indicated as normal cattle and did not carry BLAD.Conclusions: All samples in the three populations of West Java were free of BLAD genetic disorder. Identification of BLAD on HF cattle in other places, including bulls in the Center of Semen Production in Indonesia was needed to prevent this genetic disorder.
Molecular Detection of Eimeria bovis in Indonesian Beef Cattle Using Nested PCR Technique Nasrulloh, Mukh Fajar; Nurcahyo, Raden Wisnu; Priyowidodo, Dwi; Ekawasti, Fitrine; Firdausy, Lintang Winantya
HAYATI Journal of Biosciences Vol. 31 No. 5 (2024): September 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.5.980-987

Abstract

Eimeria bovis is a pathogenic protozoan that causes cattle digestive tract infections, which can cause economic losses to farmers. It is necessary to develop specific and accurate detection methods to conserve livestock and prevent coccidiosis in Indonesia. This study aims to detect E. bovis by nested PCR and determine the relationship with reference sequences. A total of 167 samples of beef cattle feces were taken randomly from community farms spread across 18 provinces in Indonesia. The feces were examined natively, and then the oocysts were purified by the sugar flotation method, extracted by KIT extraction, and amplified by the nPCR technique. Positive samples were followed by sequencing and phylogenetic analysis using MEGA 11 software. This study used two pairs of primers (outer and inner) taken from ITS-1 molecular markers. As many as 96 out of 167 samples (57.5%) were positive for Eimeria spp., and 48 of the 96 samples were positive for Eimeria spp. (50%) were detected to be positive for E. bovis based on the presence of a 238 bp DNA fragment. The results of the phylogenetic analysis showed that the study sample formed a separate cluster from the E. bovis cluster from abroad. In conclusion, E. bovis was detected in 16 out of 18 provinces in this study, and the nPCR technique proved to have better sensitivity and specificity.
Co-Authors Ari Sulistyo Wulandari Ari Sulistyo Wulandari Dwi Priyowidodo Endang Tri Margawati Endang Tri Margawati Firdausy, Lintang Winantya Fitrine Ekawasti, Fitrine Ika Rahmawati Indriawati Indriawati Indriawati Indriawati Muhammad Cahyadi Nurcahyo, Raden Wisnu Pita Sudrajad Saiful Anwar Slamet Diah Volkandari Slamet Diah Volkandari