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All Journal Makara Journal of Health Research
Paul, Mark
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Detectability of circulating microRNAs in microRNA extracts with low purity and yield using quantitative real-time polymerase chain reaction: Supporting evidence Ahmad, Azmir; Kaderi, Mohd. Arifin; Tumian, Afidalina; Sivanesan, Vijaya Mohan; Abdullah, Kahairi; Leman, Wan Ishlah; Mohamad, Irfan; Wan Zainon, Wan Mohd. Nazri; Mohd. Shiyuti, Muhammad Izani; Mohamed Awang, Kamariah; Rosla, Luqman; Paul, Mark; Syed Yussof, Sharifah Nor Ezura; Ramli, Rosdi
Makara Journal of Health Research Vol. 24, No. 3
Publisher : UI Scholars Hub

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Background: Circulating microRNAs (miRNAs) are a group of noncoding RNAs with promising potential as minimal invasive biomarkers for noncommunicable diseases. However, challenges exist in the preparation of these miRNAs from peripheral blood samples for quantification purposes. The low quality of miRNA extracts presents an obstacle. Acknowledging the superior performance of quantitative real-time polymerase chain reaction (qPCR) as gold standard for gene expression analysis, we conducted this study to observe the capabilities of qPCR using the Taqman® protocol in amplifying circulating miRNAs from miRNA extracts with low purity and yield. Methods: miRNAs were extracted from thirty-six plasma samples that were obtained from public subjects. Four selected miRNAs were quantified using the Taqman® protocol in an integrated fluidic circuit chip that was optimized from a previous study. The amplification graph and Cq values were obtained to observe any abnormal amplification signs and expression levels, respectively. Results: The qualitative observation of the amplification of the four miRNAs showed no sign of abnormality, thereby indicating the successful amplification of the miRNAs without enzymatic inhibition. Furthermore, the miRNAs were quantified in high expression levels. Conclusion: The circulating miRNA extracts with low purity and yield were practical for the study of circulating miRNA expression based on the Taqman® protocol as the method of detection.
Identification of Risk Factors Associated with Nasopharyngeal Carcinoma (NPC) in the Pahang State of Malaysia Hospitals Ahmad, Azmir; Basha, Muzaitul Akma Mustapa Kamal; Yassin, Wardah Mohd.; Rahman, Nor Azlina A.; Leman, Wan Ishlah; Rosla, Luqman; Yussof, Sharifah Nor Ezura Syed; Paul, Mark; Awang, Kamariah Mohamed; Abdullah, Kahari; Kaderi, Mohd. Arifin
Makara Journal of Health Research Vol. 26, No. 1
Publisher : UI Scholars Hub

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Background: Nasopharyngeal carcinoma (NPC) is the fifth most common cancer reported in Malaysia. Although several studies on NPC have been carried out, the risk factors associated with NPC in Malaysia are unknown. Therefore, this study was designed to investigate the risk factors associated with NPC cases in Pahang state. Methods: NPC cases that were diagnosed between 2012 and 2017 were recruited from two referral hospitals in Pahang. An interview was conducted using adapted questionnaires, which included demographic data, family history of cancer, and lifestyle. The data were analyzed statistically to identify the associations between the selected variables and NPC. Results: A total of 71 NPC cases and 81 control subjects were recruited from the hospitals. Multivariate analysis showed that a family history of NPC and current smoking were significantly associated with the risk of NPC (p < 0.05). Further analysis revealed a significant association between the risk of NPC in smokers with no family history of NPC (p < 0.05). Conclusions: This preliminary study suggests that family history and smoking are factors associated with the development of NPC in Pahang, which was consistent with previous studies.
Co-Authors Abdullah, Kahairi Abdullah, Kahari Ahmad, Azmir Awang, Kamariah Mohamed Basha, Muzaitul Akma Mustapa Kamal Kaderi, Mohd. Arifin Leman, Wan Ishlah Mohamad, Irfan Mohamed Awang, Kamariah Mohd. Shiyuti, Muhammad Izani Rahman, Nor Azlina A. Ramli, Rosdi Rosla, Luqman Sivanesan, Vijaya Mohan Syed Yussof, Sharifah Nor Ezura Tumian, Afidalina Wan Zainon, Wan Mohd. Nazri Yassin, Wardah Mohd. Yussof, Sharifah Nor Ezura Syed